Differential distribution of two ATP-gated channels (P2X receptors) determined by immunocytochemistry.

Several P2X receptor subunits were recently cloned; of these, one was cloned from the rat vas deferens (P2X1) and another from pheochromocytoma (PC12) cells differentiated with nerve growth factor (P2X2). Peptides corresponding to the C-terminal portions of the predicted receptor proteins (P2X1 391-399 and P2X2 460-472) were used to generate antisera in rabbits. The specificities of antisera were determined by staining human embryonic kidney cells stably transfected with either P2X1 or P2X2 receptors and by absorption controls with the cognate peptides. In the vas deferens and the ileal submucosa, P2X1 immunoreactivity (ir) was restricted to smooth muscle, whereas P2X2-ir was restricted to neurons and their processes. Chromaffin cells of the adrenal medulla and PC12 cells contained both P2X1- and P2X2-ir. P2X1-ir was also found in smooth muscle cells of the bladder, cardiac myocytes, and nerve fibers and terminals in the superficial dorsal horn of the spinal cord. In contrast, P2X2-ir was observed in scattered cells of the anterior pituitary, neurons in the hypothalamic arcuate and paraventricular nuclei, and catecholaminergic neurons in the olfactory bulb, the substantia nigra, ventral tegmental area, and locus coeruleus. A plexus of nerve fibers and terminals in the nucleus of the solitary tract contained P2X2-ir. This staining disappeared after nodose ganglionectomy, consistent with a presynaptic function. The location of the P2X1 subunit in smooth muscle is consistent with its role as a postjunctional receptor in autonomic transmission, while in neurons, these receptors appear in both postsynaptic and presynaptic locations.

The kappa-opioid receptor is primarily postsynaptic: combined immunohistochemical localization of the receptor and endogenous opioids.

Antisera were raised against a synthetic peptide corresponding to the carboxyl terminus of the kappa-opioid receptor (KOR1). Specificity of the antisera was verified by staining of COS-7 cells transfected with KOR1 and epitope-tagged KOR1 cDNAs, by recognition by the antisera of proteins on Western blots of both transfected cells and brain tissue, by the absence of staining of both brain tissue and transfected cells after preabsorption of the antisera with the cognate peptide, and on the strong correlation between the distribution of KOR1 immunoreactivity and that of earlier ligand binding and in situ hybridization studies. Results indicate that KOR1 in neurons is targeted into both the axonal and somatodendritic compartments, but the majority of immunostaining was seen in the somatodendritic compartment. In sections from rat and guinea pig brain, prominent KOR1 staining was seen in the ventral forebrain, hypothalamus, thalamus, posterior pituitary, and midbrain. While the staining pattern was similar in both species, distinct differences were also observed. The distribution of preprodynorphin and KOR1 immunoreactivity was complementary in many brain regions, suggesting that KOR1 is poised to mediate the physiological actions of dynorphin. However, the distribution of KOR1 and enkephalin immunoreactivity was complementary in some regions as well. These results suggest that the KOR1 protein is primarily, but not exclusively, deployed to postsynaptic membranes where it mediates the effects of products of preprodynorphin and possibly preproenkephalin.

Com fer lectors de poesia en secundària

En aquest article es presenten una sèrie d’activitats pràctiques i de reflexions amb el propòsit de fer lectors de poesia a Secundària, tot un repte per al professorat. S’hi insisteix en la necessitat de treballar a l’aula textos poètics per descobrir una altra forma d'utilitzar el llenguatge, vinculada al joc del ritme i la rima de les paraules, i perquè la poesia és indispensable per al desenvolupament intel·lectual i afectiu. Es pretén així facilitar experiències gratificants per ajudar el nostre alumnat a descobrir el gust per la lectura, l'escriptura i la dramatització de textos poètics.

1983 University of Michigan Baseball Team

Top Row: trainer Rex Thompson, C.J. Beshke, Christopher Gust, Eric Sanders, Barry Larkin, John Codere, Derek Kerr, Dale Sklar, Jeff Minick, Mark Dadabbo, asst. trainer Bill Quinn

Middle Row: coach Bud Middaugh, grad. asst. Gary Murphy, groundskeeper Willis Parrott, Dan Disher, Casey Close, Jamie Piper, Kenneth Hayward, Michael Watters, Scott Kamienicki, Kurt Zimmerman, grad asst. John Young, asst. coach Danny Hall, equip. mngr. Tom Sears

Front Row: mngr. Chris Jaksa, Richard Bair, Gary Wayne, David Knopf, Daniel Sygar, Frederick Erdman, Jeffrey Jacobson, Timothy Karazim, Richard Stoll, Charles Froning, William Shuta, Chris Sabo

1984 University of Michigan Baseball Team

Top Row: Dave Karasinski, Paul Wenson, Mike Betz, Jon Wood, Jerry Wolf, Paul Kasper, Kevin Gilles, Hal Morris, John Grettenberger, Buddy Dodge, Rob Huffman, Matt Siuda

Middle Row: equip. mngr. Jim Neidert, asst. coach Gary Murphy, Chris Gust, Barry Larkin, Eric Sanders, Casey Close, Mike Watters, Dan Disher, Kurt Zimmerman, Scott Kamieniecki, Derek Kerr, grad. Asst. John Young, trainer Rex Thompson

Front Row: asst. coach Danny Hall, Randy Wolfe, Jeff Minick, Bill Shuta, Rick Bair, Chuck Froning, Gary Wayne, Ken Hayward, C.J. Beshke, coach Bud Middaugh

1986 University of Michigan Baseball Team

Top Row: equip mngr. Dave Marich, student trainer Brian Goike, Darrin Campbell, Steve Finken, Kourtney Thompson, Tom Brook, Russ Rein, Doug Kaiser, Mike Ignasiak, Jim Durham, asst. coach Gregg Pelka

Middle Row: coach Bud Middaugh, Eddie Woodwine, Mike Gillette, John Grettenberger, Chris Lutz, Kevin Gilles, Jim Abbott, Jerry Wolf, Jeff Kiel, Bill St. Peter, asst. coach Danny Hall, asst. coach John Young

Front Row: Paul Wenson, Dave Karasinski, Matt Siuda, Derek Kerr, Kurt Zimmerman, Casey Close, Chris Gust, Scott Kamieniecki, Dan Disher, Hal Morris, Greg Everson