Characterization of S3Pvac Anti-Cysticercosis Vaccine Components: Implications for the Development of an Anti-Cestodiasis Vaccine

Background: Cysticercosis and hydatidosis seriously affect human health and are responsible for considerable economic loss in animal husbandry in non-developed and developed countries. S3Pvac and EG95 are the only field trial-tested vaccine candidates against cysticercosis and hydatidosis, respectively. S3Pvac is composed of three peptides (KETc1, GK1 and KETc12), originally identified in a Taenia crassiceps cDNA library. S3Pvac synthetically and recombinantly expressed is effective against experimentally and naturally acquired cysticercosis.Methodology/ Principal Findings: In this study, the homologous sequences of two of the S3Pvac peptides, GK1 and KETc1, were identified and further characterized in Taenia crassiceps WFU, Taenia solium, Taenia saginata, Echinococcus granulosus and Echinococcus multilocularis. Comparisons of the nucleotide and amino acid sequences coding for KETc1 and GK1 revealed significant homologies in these species. The predicted secondary structure of GK1 is almost identical between the species, while some differences were observed in the C terminal region of KETc1 according to 3D modeling. A KETc1 variant with a deletion of three C-terminal amino acids protected to the same extent against experimental murine cysticercosis as the entire peptide. on the contrary, immunization with the truncated GK1 failed to induce protection. Immunolocalization studies revealed the non stage-specificity of the two S3Pvac epitopes and their persistence in the larval tegument of all species and in Taenia adult tapeworms.Conclusions/ Significance: These results indicate that GK1 and KETc1 may be considered candidates to be included in the formulation of a multivalent and multistage vaccine against these cestodiases because of their enhancing effects on other available vaccine candidates.

Sympathetic mediation of salivation induced by intracerebroventricular pilocarpine in rats

Central cholinergic activation by pilocarpine induces salivation dependent on the integrity of forebrain areas. The present work investigates the autonomic mediation of this salivation. Pilocarpine (500 nmol/rat) was injected into the lateral ventricle (LV) of tribromoethanol-anesthetized adult male rats. Preweighed cotton balls were inserted into the oral cavity and weighed again 7 min later. ol-adrenoceptor antagonists (3-50 mu mol/kg) prazosin (alpha(1)), yohimbine (alpha(2)) or propranolol (beta) injected intraperitoneally (i.p.) produced, 80%, 20% and 0% inhibition respectively of the LV pilocarpine-induced salivation. Intracerebroventricular injections (160 nmol) of the antagonists did not alter the effects of pilocarpine injected into the LV. Bilateral section of chorda tympani nerve or bilateral sympathetic cervical ganglionectomy produced 0% and 40% inhibition of pilocarpine-induced salivation, respectively. Ganglionectomy did not alter salivation induced by i.p, injection of pilocarpine (4 mu mol/kg). The results indicate that there is a large sympathetic contribution to the salivation induced by central cholinergic activation. (C) 1999 Elsevier B.V. B.V. All rights reserved.

La banca de desarrollo en México

Incluye Bibliografía

Bolivia: equidad y grupos de interés en la reforma educativa

Incluye Bibliografía

La ciudad de Cochabamba

Los documentos del Seminario fueron publicados por UNESCO en 1961 con el título: La urbanización en América Latina/Urbanization in Latin America

Judeoconversos e Inquisición en las islas Canarias(1402-1605)

Doctorado en Historia. Programa de España y Canarias en la Historia Moderna y Contemporánea

Piédrola: avance de las intervenciones (2013-2014)

[ES]El paraje de Piédrola se localiza a seis kilómetros de distancia al noroeste de la localidad de Alcázar de San Juan (Ciudad Real), en dirección al vecino municipio toledano de Quero. En este lugar se localiza uno de los yacimientos arqueológicos más importantes de la comarca, al menos en extensión y cronología. Éste toma su nombre del topónimo de la zona: Piédrola. Los trabajos arqueológicos acometidos desde 2013 en el lugar nos permiten trazar una primera aproximación a la historia de este singular paraje.

DNA hybrid materials consisting of oligonucleotides and organic molecules: synthesis, characterization and applications

The goal of this thesis was to increase the functionality of pristine DNA scaffolds by functionalizing them with fluorescent dyes and hydrophobic moieties. Two important steps were necessary to realize this aim successfully. First, nucleic acids needed to be synthesized making use of multidisciplinary toolbox for the generation and manipulation of polynucleic acids. The most important techniques were the solid phase synthesis involving the incorporation of standard and modified phosphoramidite building blocks as well as molecular biology procedures like the polymerase chain reaction, the bacterial amplification of plasmids and the enzymatic digestion of circular vectors. Second, and evenly important, was the characterization of the novel bioorganic hybrid structures by a multitude of techniques, especially optical measurements. For studying DNA-dye conjugates methods like UV/Vis and photoluminescence spectroscopy as well as time resolved luminescence spectroscopy were utilized. While these measurements characterized the bulk behavior of an ensemble of DNA-dye hybrids it was necessary for a complete understanding of the systems to look at single structures. This was done by single-molecule fluorescence spectroscopy and fluorescence correlation spectroscopy. For complete analysis the optical experiments were complemented by direct visualization techniques, i.e. high resolution transmission electron microscopy and scanning force microscopy.

El país de Juan di María Teresa Andruetto: un “crinale inesplorato” tra sfide e conquiste

Il presente elaborato si propone di analizzare il racconto dalla scrittrice argentina María Teresa Andruetto (Premio Hans Christian Andersen 2012) El país de Juan, nell’edizione pubblicata dalla casa editrice spagnola Anaya nel 2003. Nel 2014 ne è stata proposta la traduzione in italiano con il titolo Il paese di Juan, ad opera di Ilide Carmignani. La storia, che narra di due bambini costretti a migrare dalla campagna alle favelas argentine (Villas), si presenta come una vera e propria sfida traduttiva per il contrasto tra la poeticità del linguaggio e il realismo del mondo raccontato ed il forte legame con il contesto locale in cui è inserita. Lo studio consta di tre capitoli principali: nel primo si approfondiscono la vita, le opere, lo stile e la concezione della letteratura della Andruetto. Dopo aver impostato tale cornice stilistica e contestuale, si prosegue, nel secondo capitolo, con l’analisi del testo originale, dove vengono messi in luce gli aspetti formali e stilistici della scrittura, le tematiche, gli elementi grafici, le illustrazioni e il contesto storico-sociale. Si passa poi, nel terzo ed ultimo capitolo, ad un’analisi della traduzione italiana del libro rispetto alla capacità di ricreare un tipo di linguaggio altamente innovativo, la salvaguardia delle figure retoriche, degli aspetti grafici e dei riferimenti culturali, elementi di fondamentale importanza per creare una traduzione in grado di aprire gli orizzonti del giovane lettore. Inoltre, si confrontano i glossari della versioni spagnola e italiana evidenziandone differenze e affinità. Si pone infine l’accento sull’indiscutibile abilità della Carmignani nel saper mantenere la dicotomia testuale tra realismo e mistero, riferendosi anche all’importanza della collaborazione tra lo scrittore e il traduttore, legame che senza dubbio è possibile cogliere nella traduzione Il paese di Juan.

The Effect of College Student Demographic Variables on Teacher Enmeshment

Interactions between students and faculty outside of class appear to be linked to greater achievement during and after college (Anaya & Cole, 2001; Hathaway, Nagda, & Gregerman, 2002). However, sometimes there can be blurred personal boundaries and a lack of autonomy in relationships or what has been labeled enmeshment. The purpose of the current pilot study was to investigate the effect of race/ethnicity, gender, year in college, and college major on faculty-student relationships and teacher enmeshment. Teacher enmeshment was measured with the Teacher Enmeshment subscale of the Separation-Individuation Test of Adolescence (SITA; Levine & Saintonge, 1993). A sample of 165 undergraduate and graduate students from education and psychology classes at a small, private liberal arts institution in the Northeast participated. No significant differences among the different demographic groups were found on the total teacher enmeshment score. However, significant differences were found among students with different majors, by gender, and by race on individual items. Implications of these findings and suggestions for future research are provided.

Aedes aegypti, Aedes albopictus, and Dengue virus in Harris county: An estimate of risk

Recent outbreaks of dengue fever (DF) along the United States/Mexico border, coupled with the high number of reported cases in Mexico suggest that there is the possibility for DF emergence in Houston, Texas1,2. To determine the presence of DF, populations of Aedes aegypti and Aedes albopictus were identified and tested for dengue virus. Maps were created to identify "hot spots" (Figure 1) based on historical data on Ae. aegypti and Ae. albopictus, demographic information, and locations of human cases of dengue fever. BG Sentinel Traps®, in conjunction with BG Lure® attractant, octanol and dry ice, were used to collect mosquitoes, which were then tested for presence of dengue virus using ELISA techniques. All samples tested were negative for dengue virus (DV). Survival of DV ultimately comes down to whether or not it will be vectored by a mosquito to a susceptible human host. The presence of infected humans and contact with the mosquito vectors are two critical factors necessary in the establishment of DF. Historical records indicate the presence of Ae. aegypti and Ae. albopictus in Harris County, which would support localized dengue transmission if infected individuals are present.^ (1) Brunkard JM, Robles-Lopez JL, Ramirez J, Cifuentes E, Rothenberg SJ, Hunsperger EA, Moore CG, Brussolo RM, Villarreal NA, Haddad BM, 2007. Dengue fever seroprevalence and risk factors, Texas-Mexico border, 2004. Emerg Infect Dis 13: 1477-1483. (2) Ramos MM, Mohammed H, Zielinski-Gutierrez E, Hayden MH, Lopez JL, Fournier M, Trujillo AR, Burton R, Brunkard JM, Anaya-Lopez L, Banicki AA, Morales PK, Smith B, Munoz JL, Waterman SH, 2008. Epidemic dengue and dengue hemorrhagic fever at the Texas-Mexico Border: results of a household-based seroepidemiologic survey, December 2005. Am J Trop Med Hyg 78: 364-369.^

Formulación de un plan de desarrollo agropecuario y forestal para una comunidad ejidal del municipio de San Fernando, Chiapas, México

El perfil socioeconómico de Chiapas es primordialmente rural, debiendo reconocerse la diversidad de sus actividades agropecuarias y forestales para generar condiciones específicas de desarrollo. Considerando lo anterior, se realizó esta investigación entre mayo de 1998 y diciembre de 1999, como un estudio de caso sobre los aspectos agropecuarios y forestales más relevantes en el ejido Villa Allende, que expresa problemas ejidales característicos de la región. El ejido cuenta con 3.300 hectáreas, 554 ejidatarios y se ubica en el municipio de San Fernando en la Región Central de Chiapas. Se estudiaron las circunstancias de los ejidatarios, conocimiento local y procesos de trabajo agrícola desde una perspectiva de planeación. A partir de ello se generó el plan de desarrollo, el cual es el primero expuesto en el municipio de San Fernando que se dirige al sector ejidal. Su propósito es plasmar una propuesta general en materia agropecuaria y forestal que pueda ser base para la concertación y que permita vislumbrar opciones dirigidas a mejorar la calidad de vida de los habitantes del ejido Villa Allende. Se postulan las siguientes políticas de desarrollo: 1. Fortalecimiento de la producción y la autosuficiencia alimentaria. 2. Impulso al crecimiento económico. 3. Dotación de infraestructura básica. 4. Reordenamiento agropecuario y forestal con criterios sociales, ecológicos y económicos. 5. Promoción de la salud. Con base en las políticas, se enuncian objetivos programas y subprogramas o proyectos.

Formulación de un plan de desarrollo agropecuario y forestal para una comunidad ejidal del municipio de San Fernando, Chiapas, México

El perfil socioeconómico de Chiapas es primordialmente rural, debiendo reconocerse la diversidad de sus actividades agropecuarias y forestales para generar condiciones específicas de desarrollo. Considerando lo anterior, se realizó esta investigación entre mayo de 1998 y diciembre de 1999, como un estudio de caso sobre los aspectos agropecuarios y forestales más relevantes en el ejido Villa Allende, que expresa problemas ejidales característicos de la región. El ejido cuenta con 3.300 hectáreas, 554 ejidatarios y se ubica en el municipio de San Fernando en la Región Central de Chiapas. Se estudiaron las circunstancias de los ejidatarios, conocimiento local y procesos de trabajo agrícola desde una perspectiva de planeación. A partir de ello se generó el plan de desarrollo, el cual es el primero expuesto en el municipio de San Fernando que se dirige al sector ejidal. Su propósito es plasmar una propuesta general en materia agropecuaria y forestal que pueda ser base para la concertación y que permita vislumbrar opciones dirigidas a mejorar la calidad de vida de los habitantes del ejido Villa Allende. Se postulan las siguientes políticas de desarrollo: 1. Fortalecimiento de la producción y la autosuficiencia alimentaria. 2. Impulso al crecimiento económico. 3. Dotación de infraestructura básica. 4. Reordenamiento agropecuario y forestal con criterios sociales, ecológicos y económicos. 5. Promoción de la salud. Con base en las políticas, se enuncian objetivos programas y subprogramas o proyectos.