Cyclodextrin Production by Bacillus lehensis Isolated from Cassava Starch: Characterisation of a Novel Enzyme

The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics (optimum pH and temperature; pH and temperature stability), the influence of substances on the enzyme activity, enzyme kinetics, and cyclodextrin production were analysed. Cyclodextrin glycosyltransferase was purified up to 320.74-fold by affinity chromatography using beta-cyclodextrin as the binder and it exhibited 8.71% activity recovery. This enzyme is a monomer with a molecular weight of 81.27 kDa, as estimated by SDS-PAGE. Optimum temperature and pH for cydodextrin glycosyltransferase were 55 degrees C and 8.0, respectively. The Michaelis-Menten constant was 8.62 g/l during maximum velocity of 0.858 g/l.h.

Questionário de qualidade de vida de crianças com paralisia cerebral (cp qol-child): tradução e adaptação para língua portuguesa

The aim of this study was to verify the reliability of the Brazilian Portuguese version of the instrument Cerebral Palsy: quality of life questionnaire for children: primary caregiver questionnaire (CP QOLChild: primary caregiver) translated and culturally adapted. Thus, the translation and cultural adaptation of the instrument was made, and then the procedures to verify its reliability were performed. The translated and culturally adapted questionnaire was answered by 30 caregivers of children with cerebral palsy and inter and intra-observer analysis followed. The data allowed the authors to identify an internal consistency rangering from 0.649 to 0.858, intra-observer reliability from 0.625 to 0.809 and inter-observer reliability from 0.498 to 0.903. The analysis suggests that the instrument has acceptable psychometry.

Desempenho cognitivo-linguístico e leitura em escolares com transtorno do déficit de atençào e hiperatividade

OBJECTIVE: the aim of this study was to compare the cognitive-linguistic skills performance and reading of students with Attention Deficit and Hyperactivity Disorder and students without behavioral and/or learning disorders. METHOD: the study included 20 students from 5th to 8th grade of elementary school. The students were divided into: Group I (GI): composed by 10 students with an interdisciplinary diagnosis of Attention Deficit and Hyperactivity Disorder, and Group II (GII): composed by 10 students without complaints of behavioral and/or learning disorders. The tests of metalinguistic skills and reading (PROHMELE) were used as procedure, composed of syllabic and phonemic identification, syllabic and phonemic manipulation, repetition of nonwords and reading tests. RESULTS: the results showed statistically significant differences between GI and GII, demonstrating that students from GI presented superior performances when compared to the students from GII. CONCLUSION: according to the findings of this study we can conclude that the difficulties presented by students with Attention Deficit and Hyperactivity Disorder can be attributed to inattention, hyperactivity and disorganization, characteristic of this diagnosis, and not to a disorder of language of phonological basis.

Study of thermal decomposition of ignition temperature of bagasse, coal and their blends

In Brazil, due to its availability, sugar cane bagasse has a high potential for power generation. The knowledge of ignition behavior, as well as the knowledge of the chemical kinetics, in of fuels combustion process is important features in boilers projects and in the stability of the combustion process control. The aim of this study is to investigate the thermal behavior of sugar cane bagasse, coal and their blends. The methodology proposed by Tognotti et al. (1985) was applied to determine the ignition temperature for all samples. Ignition temperatures were 256oC for neat bagasse and 427oC for neat coal, and 275oC for both blends (50-50% and 25-75%). The ModelFree Kinetics was applied to determine the apparent activation energy (Eα) of the thermal decomposition of sugar cane bagasse. For the two major events of mass loss of bagasse which correspond to the thermal decomposition of organic matter (mainly hemicellulose, cellulose and lignin), average values of Eα were obtained for both combustion and pyrolysis processes. In synthetic air atmosphere, the Eα were 170.8±26.3 kJ⋅mol-1 and 277.8±58.6 kJ⋅mol-1, while in nitrogen atmosphere, the Eα were 185.0 ± 11.4 kJ⋅mol-1 and 82.1±44.4 kJ⋅mol-1. The results obtained can be explained by synergistic effects when both bagasse and coal were blended, changing the fuel reactivity.

Habilidades sociais e características pessoais em escolares de Belém

Este estudo relacionou a frequência da emissão de reação socialmente habilidosa, não-habilidosa passiva e não-habilidosa ativa às variáveis da criança (sexo, idade, condições clínicas). Participaram 57 meninos e 52 meninas, entre seis e 12 anos. O Questionário de Caracterização da Criança (QCC) levantou informações sobre sexo, idade e condições clínicas e o Inventário Multimídia de Habilidades Sociais de Crianças (IMHSC-Del Prette) avaliou as habilidades sociais, respondidos pelos cuidadores e escolares, respectivamente. Utilizou-se os testes U de Mann-Whitney, o coeficiente de correlação de Pearson e o teste t para análises dos dados. Os resultados indicaram: (a) diferenças significativas na adequação das reações habilidosas e não-habilidosas (p < 0,001); (b) aumento do repertório de habilidades sociais conforme o avançar dos anos (p < 0,001); e (c) meninas apresentaram mais frequência de habilidades sociais que meninos (p = 0,040). Não houve associações significativas entre habilidades sociais e condições clínicas (p = 0,539). Verificou-se que sexo e idade podem interferir na presença e desempenho de repertório socialmente habilidoso. Sugerem-se outras técnicas de avaliação que complementem os dados investigados e possibilitem intervenções futuras para amostras semelhantes.

Review of The Elemental Prairie: Sixty Tallgrass Plants (Watercolors and introduction) by George Olson

The Elemental Prairie provides a general discussion of the Great Plains and the tallgrass prairie for the general reader. Its botanically accurate plant drawings render a beautiful and artistic view into prairie plants. George Olson writes a compelling introduction about "Prairie Elements," painting a graphic verbal description about his trip into the prairie with noted prairie author John Madson. The introduction draws readers into the book and prepares them for John Madson's essay "The Running Country," an eloquent portrayal of the history of the tallgrass prairie. We are led into the hearts and minds of the pioneers who crossed the immense expanse of the Great Plains. Madson's descriptions of prairie plants help us visualize how the Great Plains looked prior to settlement, stirring us to see not only the allure of the prairies, but also the solitude and sometimes the loneliness. Madson mixes his personal experiences with current scientific theory of the formation of the prairies across the region, offering a way of seeing how the present fits into the past.

Selective Foraging For Anthropogenic Resources By Black Bears: Minivans In Yosemite National Park

Black bears (Ursus americanus) forage selectively in natural environments. To determine if bears also forage selectively for anthropogenic resources we analyzed data on vehicles broken into by bears from Yosemite National Park, California. We classified vehicles into 9 categories based on their make and model and collected data on use (2001–2007) and availability (2004–2005). From 2001 to 2007 bears broke into 908 vehicles at the following rates: minivan (26.0%), sport–utility vehicle (22.5%), small car (17.1%), sedan (13.7%), truck (11.9%), van (4.2%), sports car (1.7%), coupe (1.7%), and station wagon (1.4%). Only use of minivans (29%) during 2004–2005 was significantly higher than expected (7%). We discuss several competing hypotheses about why bears selected minivans.

Computational tools for comparing asymmetric GARCH models via Bayes factors

In this paper we use Markov chain Monte Carlo (MCMC) methods in order to estimate and compare GARCH models from a Bayesian perspective. We allow for possibly heavy tailed and asymmetric distributions in the error term. We use a general method proposed in the literature to introduce skewness into a continuous unimodal and symmetric distribution. For each model we compute an approximation to the marginal likelihood, based on the MCMC output. From these approximations we compute Bayes factors and posterior model probabilities. (C) 2012 IMACS. Published by Elsevier B.V. All rights reserved.

Negative and positive predictive values of nerve monitoring in thyroidectomy

Background Recurrent nerve injury is 1 of the most important complications of thyroidectomy. During the last decade, nerve monitoring has gained increasing acceptance in several centers as a method to predict and to document nerve function at the end of the operation. We evaluated the efficacy of a nerve monitoring system in a series of patients who underwent thyroidectomy and critically analyzed the negative predictive value (NPV) and positive predictive value (PPV) of the method. Methods. NIM System efficacy was prospectively analyzed in 447 patients who underwent thyroidectomy between 2001 and 2008 (366 female/81 male; 420 white/47 nonwhite; 11 to 82 years of age; median, 43 years old). There were 421 total thyroidectomies and 26 partial thyroidectomies, leading to 868 nerves at risk. The gold standard to evaluate inferior laryngeal nerve function was early postoperative videolaryngoscopy, which was repeated after 4 to 6 months in all patients with abnormal endoscopic findings. Results. At the early evaluation, 858 nerves (98.8%) presented normal videolaryngoscopic features after surgery. Ten paretic/paralyzed nerves (1.2%) were detected (2 unexpected unilateral paresis, 2 unexpected bilateral paresis, 1 unexpected unilateral paralysis, 1 unexpected bilateral paralyses, and 1 expected unilateral paralysis). At the late videolaryngoscopy, only 2 permanent nerve paralyses were noted (0.2%), with an ultimate result of 99.8% functioning nerves. Nerve monitoring showed absent or markedly reduced electrical activity at the end of the operations in 25/868 nerves (2.9%), including all 10 endoscopically compromised nerves, with 15 false-positive results. There were no false-negative results. Therefore, the PPV was 40.0%, and the NPV was 100%. Conclusions. In the present series, nerve monitoring had a very high PPV but a low NPV for the detection of recurrent nerve injury. (C) 2011 Wiley Periodicals, Inc. Head Neck 34: 175-179, 2012

Impact of Aqueous Plant Extracts on Trigona spinipes (Hymenoptera: Apidae)

The stingless bees are an important component of the insect biomass in many tropical areas, due to their collection of nectar and pollen. Trigona spinipes is a widely distributed species in South America, and described as a pollinator of many crops that can be used in a commercial pollinating system. The effects of plant extracts on insects are studied because of the demand for organic food and their selectivity to natural enemies. Plant insecticides are reported as a potential agent for the control of insect pests, however little is known about their impact on beneficial insects. This study investigated the survival of Trigona spinipes (Hymenoptera: Apidae, Meliponini) Fabricius, after exposure to the leaf extracts of Azadiracha indica (Meliaceae), Lippia sidoides (Verbenaceae), Sapindus saponaria (Sapindaceae), Anonna squamosa (Anonnaceae) Cymbopogon winterianum (Poaceae), Corimbia citriodora (Myrtaceae), Jatropha curcas (Euphorbiaceae) and Ricinus communis (Euphorbiaceae) and of seeds of Azadiracha indica, Ricinus communis Nordestina and AL Guarany varieties and Jatropha curcas. The extracts that had the greatest influence on the survival of the bees were A. indica at 3% and 7% of concentration, A. squamosa at a concentration of 10% with 68.89% survival and green leaf of R. communis at a concentration of 7%. The results show that although the extracts were effective in controlling pests, they may also affect the pollinator Trigona spinipes.

A Homonuclear Rotational Echo Double-Resonance Method for Measuring Site-Resolved Distance Distributions in I=1/2 Spin Pairs, Clusters, and Multispin Systems

Deutsche Forschungsgemeinschaft [SFB 858]

Gauge and integrable theories in loop spaces

We propose an integral formulation of the equations of motion of a large class of field theories which leads in a quite natural and direct way to the construction of conservation laws. The approach is based on generalized non-abelian Stokes theorems for p-form connections, and its appropriate mathematical language is that of loop spaces. The equations of motion are written as the equality of a hyper-volume ordered integral to a hyper-surface ordered integral on the border of that hyper-volume. The approach applies to integrable field theories in (1 + 1) dimensions, Chern-Simons theories in (2 + 1) dimensions, and non-abelian gauge theories in (2 + 1) and (3 + 1) dimensions. The results presented in this paper are relevant for the understanding of global properties of those theories. As a special byproduct we solve a long standing problem in (3 + 1)-dimensional Yang-Mills theory, namely the construction of conserved charges, valid for any solution, which are invariant under arbitrary gauge transformations. (C) 2012 Elsevier B.V. All rights reserved.

Natural fiber-reinforced thermoplastic starch composites obtained by melt processing

Thermoplastic starch (TPS) from industrial non-modified corn starch was obtained and reinforced with natural strands. The influence of the reinforcement on physical-chemical properties of the composites obtained by melt processing has been analyzed. For this purpose, composites reinforced with different amounts of either sisal or hemp strands have been prepared and evaluated in terms of crystallinity, water sorption, thermal and mechanical properties. The results showed that the incorporation of sisal or hemp strands caused an increase in the glass transition temperature (T-g) of the TPS as determined by DMTA. The reinforcement also increased the stiffness of the material, as reflected in both the storage modulus and the Young's modulus. Intrinsic mechanical properties of the reinforcing fibers showed a lower effect on the final mechanical properties of the materials than their homogeneity and distribution within the matrix. Additionally, the addition of a natural latex plasticizer to the composite decreased the water absorption kinetics without affecting significantly the thermal and mechanical properties of the material. (c) 2012 Elsevier Ltd. All rights reserved.

Classifying A-field and B-field configurations in the presence of D-branes. Part II: Stacks of D-branes

In the paper of Bonora et al. (2008) [3] we have shown, in the context of type II superstring theory, the classification of the allowed B-field and A-field configurations in the presence of anomaly-free D-branes, the mathematical framework being provided by the geometry of gerbes. Here we complete the discussion considering in detail the case of a stack of D-branes, carrying a non-abelian gauge theory, which was just sketched in Bonora et al. (2008) [3]. In this case we have to mix the geometry of abelian gerbes, describing the B-field, with the one of higher-rank bundles, ordinary or twisted. We describe in detail the various cases that arise according to such a classification, as we did for a single D-brane, showing under which hypotheses the A-field turns out to be a connection on a canonical gauge bundle. We also generalize to the non-abelian setting the discussion about "gauge bundles with non-integral Chern classes", relating them to twisted bundles with connection. Finally, we analyze the geometrical nature of the Wilson loop for each kind of gauge theory on a D-brane or stack of D-branes.

What is Missing for Traditional Design-Centric Engineering Education to Better Prepare Newly Graduated Engineers for the Global Era?

This paper presents an analysis of the capacity of design centric methodologies to prepare engineering students to succeed in the market. Gaps are brainstormed and analyzed with reference to their importance. Reasons that may lead the newly graduated engineers not to succeed right from the beginning of their professional lives have also been evaluated. A comparison among the two subjects above was prepared, reviewed and analyzed. The influence of multidisciplinary, multicultural and complex environmental influences created in the current global business era is taken into account. The industry requirements in terms of what they expect to 'receive' from their engineers are evaluated and compared to the remaining of the study above. An innovative approach to current engineering education that utilizes traditional design-centric methodologies is then proposed, aggregating new disciplines to supplement the traditional engineering education. The solution encompasses the inclusion of disciplines from Human Sciences and Emotional Intelligence fields willing to better prepare the engineer of tomorrow to work in a multidisciplinary, globalized, complex and team working environment. A pilot implementation of such an approach is reviewed and conclusions are drawn from this educational project.