Use of comet assay to assess DNA damage in patients infected by Helicobacter pylori - Comparisons between visual and image analyses

Studies of DNA damage in gastric epithelial cells of Helicobacter pylori (H. pylori)-infected patients are conflicting, possibly due to different methods used for scoring DNA damage by Comet assay. Therefore, we compared the sensitivity of visual microscopic analysis (arbitrary units-scores and comets%) and image analysis system (tail moment), in the gastric epithelial cells from the antrum and corpus of 122 H. pylori-infected and 32 non-infected patients. The feasibility of cryopreserved peripheral blood lymphocytes and whole-blood cells for DNA damage biomonitoring was also investigated. In the antrum, the levels of DNA damage were significantly higher in H. pylori-infected patients with gastritis than in non-infected patients with normal mucosa, when evaluated by image analysis system, arbitrary units and comets%. In the corpus, the comets% was not sufficiently sensitive to detect the difference between H. pylori-infected patients with gastritis and non-infected patients with normal mucosa. The image analysis system was sensitive enough to detect differences between non-infected patients and H. pylori-infected patients with mild gastritis and between infected patients with moderate and severe gastritis, in both antrum, and corpus, while arbitrary units and comets% were unable to detect these differences. In cryopreserved peripheral blood lymphocytes, the levels of DNA damage (tail moment) were significantly higher in H. pylori-infected patients with moderate and severe gastritis than in non-infected patients. Overall, our results indicate that the image analysis system is more sensitive and adequate to measure the levels of DNA damage in gastric epithelial cells than the other methods assayed. (c) 2005 Elsevier B.V. All rights reserved.

Crítica periodística: a mediação das artes visuais na Folha de São Paulo e no Estado de São Paulo durante a 29a. Bienal de São Paulo (25/09 a 12/12 de 2010

Pós-graduação em Artes - IA

Search for a non-standard-model Higgs boson decaying to a pair of new light bosons in four-muon final states

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Interferência psicológica em equipes que disputam campeonatos com mando de jogo: o caso do futsal/UNESP 09

Como a busca por vitórias se torna cada vez mais difícil no esporte coletivo, qualquer vantagem é vista como um diferencial em cada partida. Além da vantagem física e tática, que seriam os mais tradicionais, os treinadores buscam outros meios como vantagem psicológica ou jogar com o mando de campo, que para muitos não passa de ter a torcida a favor, mas para os jogadores e treinadores a torcida tem papel importante de incentivar e para os adversários os deixar nervosos, também não se esquecendo da vantagem de conhecer e treinar no local do jogo alem de poupar os atletas do desgaste de viagens. Este trabalho visa verificar como o mando de campo interfere nos atletas de futsal, alem de como as variadas torcidas influenciam os jogadores durante a partida e como os atletas vêem seus companheiros de time. Foi realizado uma pesquisa bibliográfica e de campo, pela técnica de questionário fechado, que possibilitou a compreensão do fato em estudo, ampliando nossa visão. No decorrer de nosso estudo constatamos que de maneira geral jogar em casa é fator determinante para o resultado final da partida, o que menos motiva os atletas é a possibilidade do juiz ser influenciado pela torcida local e que o grupo masculino é mais fácil de ser motivado do que o grupo feminino. Com os resultados obtidos o treinador passa a conhecer melhor seus jogadores, podendo assim, talvez, escalar o time melhor em momentos difíceis.

Draft genome sequence of shiga toxin-producing Escherichia coli Strain D92/09

Escherichia coli is suspected to be involved with Crohn's disease. Adherence and invasion to epithelial cells are properties commonly observed in these bacteria. Here, we present a draft genome sequence of E. coli D92/09, a multidrug-resistant strain, which besides showing these properties produces Shiga cytotoxin-1 and possibly other toxins.

Inhibition of thimet oligopeptidase by siRNA alters specific intracellular peptides and potentiates isoproterenol signal transduction

Mammalian cells have a large number of intracellular peptides that are generated by extralysosomal proteases. In this study, the enzymatic activity of thimet oligopeptidase (EP24.15) was inhibited in human embryonic kidney (HEK) 293 cells using a specific siRNA sequence. The semi-quantitative intracellular peptidome analyses of siRNA-transfected HEK293 cells shows that the levels of specific intracellular peptides are either increased or decreased upon EP24.15 inhibition. Decreased expression of EP24.15 was sufficient to potentiate luciferase gene reporter activation by isoproterenol (1-10 mu M). The protein kinase A inhibitor KT5720 (1 mu M) reduced the positive effect of the EP24.15 siRNA on isoproterenol signaling. Thus, EP24.15 inhibition by siRNA modulates the levels of specific intracellular peptides and isoproterenol signal transduction. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.