Ciclo de muda e metabolismo durante o desenvolvimento larval do camarão-da-amazônia Macrobrachium amazonicum (Heller, 1862)

Pós-graduação em Aquicultura - FCAV

Níveis de cálcio, fósforo, lipídeo e proteína na dieta inerte do camarão-da-malásia Macrobrachium rosenbergii, na fase larval

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Tecnologia pós-despesca dos camarões de água doce Macrobrachium rosenbergii e Macrobrachium amazonicum

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fecundidade e fertilidade do camarão-da-Amazônia, Macrobrachium amazonicum (Heller, 1862) (Decapoda: Palaemonidae) em dois ambientes estuarinos do estado do Pará

O Macrobrachium amazonicum é o camarão de água doce com ampla exploração pesqueira na região amazônica, em virtude da abundância e boa aceitação no mercado consumidor. O objetivo deste estudo foi caracterizar a fecundidade e fertilidade de M. amazonicum em dois ambientes estuarinos no Estado do Pará. De forma que foram realizadas coletas mensais no período de setembro de 2008 a agosto de 2009, nos município de Vigia e Belém na Ilha de Mosqueiro, as fêmeas foram até o Laboratório de Ecologia Aquática e Aqüicultura Tropical - LECAT no campus da Universidade Federal Rural da Amazônia – UFRA, em Belém. Para a determinação da fecundidade foram colecionadas mensalmente em média 30 fêmeas ovígeras de cada ponto de coleta, os ovos aderidos aos pleópodes foram retirados, utilizando hipoclorito de sódio como 2% de cloro ativo e estocado em álcool 70%. A fecundidade individual foi determinada a partir da contagem total dos ovos. Para a determinação do volume dos ovos foram selecionados de cada local de coleta, aleatoriamente, 50% do número total de fêmeas ovígeras destinadas a fecundidade, seguindo dois grupos: ovos pigmentados e não-pigmentados. Para determinar a fertilidade, as fêmeas ovígeras de M. amazonicum foram estocadas individualmente em aquários de vidro de 2L. Após a eclosão, as larvas foram sifonadas e contadas. Para todas as fêmeas foi aferido o peso de cada exemplar e avaliada sua biometria com o auxílio de um paquímetro de precisão. As fêmeas de M. amazonicum oriundas do Município de Vigia tiveram comprimento absoluto entre 4,8 e 9,3 cm e peso entre 2,21 e 11,81g, com fecundidade absoluta entre 38 e 5.749 ovos (2.296 ± 1.288 ovos). Para as fêmeas de Belém, provenientes da ilha de Mosqueiro, o comprimento variou entre 3,71 e 8,14 cm, o peso entre 1,27 e 11,2g, com fecundidade absoluta variando de 123 e 7.571, com média de 1.448 ± 990 ovos. O volume de ovos para as fêmeas obtidas no Município de Vigia, referente aos ovos não-pigmentados, apresentou volume médio de 141,37mm³, enquanto que para ovos pigmentados o volume foi de 116,13mm³. As fêmeas provenientes da Ilha de Mosqueiro, com ovos não-pigmentados apresentaram volume médio de 118,97mm³ e ovos pigmentados o volume médio calculado foi de 144,61mm³. A análise da fertilidade para as fêmeas do município de Vigia apresentou comprimento entre 5,41 e 9,72 cm e peso entre 2,51 e 9,60g, a fertilidade absoluta foi 14 e 4.430 larvas, com média de 1.152 ± 822 larvas por fêmea. As da ilha de Mosqueiro apresentaram comprimento entre 3,53 e 7,67 cm e peso entre 1,12 e 8,77 g com fertilidade absoluta entre 7 e 4.121 e média de 755 ± 871 larvas por fêmea. Os valores referentes a fertilidade e fecundidade para os município de Vigia e Belém - Ilha de Mosqueiro indicam que há proporcionalidade entre as variáveis de comprimento e peso. Apesar de apresentar fecundidade e fertilidade menor que os valores reprodutivos apresentados para outras espécies de interesse comercial, os valores aqui mostrados evidenciam que nestes dois locais a espécie podem fornecer matrizes potenciais para aquicultura.

Efeito da diversidade de habitats sobre o desenvolvimento populacional do camarão-da-amazônia

Pós-graduação em Aquicultura - FCAV

Chemical composition and fatty acid contents in farmed freshwater prawns

The objective of this work was to evaluate the chemical composition and fatty acid contents of Amazonian and giant river prawns. After four-month farming, with the same diet for both species, palmitic and stearic acids were the main saturated fatty acids. Oleic acid was the main monounsatured fatty acid, and the eicosapentaenoic and docosahexaenoic acids were the most abundant polyunsaturated acids. Amazonian prawn has higher levels of protein and polyunsaturated fatty acids than those of the giant river prawn, which shows its potential for aquaculture.

Predação de ovócitos de Piaractus mesopotamicus (pacu-caranha) por Macrobrachium amazonicum em condições laboratoriais

The preference of predation on oocytes of Piaractus mesopotamicus, commonly known as 'Pacucaranha', by the prawn Macrobrachium amazonicum, under laboratory conditions, was evaluated regarding to other food items. The prawns were collected with sieves, placed below the underwater vegetation, and maintained in aquarium (12 L each) at a density of 10 individuals/aquarium for acclimatization. Oocytes (O) of P. mesopotamicus were obtained from the disposal of artisanal fishery. Artificial feed (R), fish muscle (M), organic matter accumulated in roots of Salvinia sp. (S) and plant organic matter in decomposition (MV) items were used to compare the preference by M. amazonicum. In all experiments the average of the prawns preying oocytes were higher; significant differences were detected only when comparing O/P and O/M. The preference for oocytes may be due to the presence of yolk, which has a high nutritional value and easy assimilation, in contrast to others items, which resent certain rigidity. This prawn species is exotic on site and the results of this study shows a possible environmental impact on P. mesopotamicus in the region.

A test of large-scale reproductive migration in females of the amphidromous shrimp Macrobrachium acanthurus (Caridea : Palaemonidae) from south-eastern Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fecundity and fertility in a freshwater population of the neotropical amphidromous shrimp Macrobrachium acanthurus from the southeastern Atlantic

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análises isoenzimáticas, moleculares e ultraestruturais em camarões dulcícolas do gênero Macrobrachium

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ocurrence of male morphotypes of Macrobrachium amazonicum (Caridea, Palaemonidae) in a population with an entirely freshwater life cycle

This study records, for the first time, the occurrence of all four male morphotypes in a population of Macrobrachium amazonicum from a continental environment, with an entirely freshwater life cycle. The specimens studied came from the Tietê River, state of São Paulo, Brazil, and were collected in a lotic environment downstream from Ibitinga Dam. This population was compared with other continental populations, including a population from the dam itself, collected in a previous study. Four samples of 30 minutes were taken monthly, using a trap, from January to April 2011. Each male specimen was measured with respect to seven body dimensions as follows: carapace length (CL), right cheliped length (RCL), dactyl length (DCL), propodus length (PPL), carpus length (CRL), merus length (ML) and ischium length (IL). The relative growth was analyzed based on the change in growth patterns of certain body parts in relation to the independent variable CL. The four male morphotypes proposed for the species were found using morphological and morphometric analyses. Different biological characteristics were found between the populations studied. The male population of the lake of Ibitinga and from Pantanal presented mean sizes and number of morphotypes lower than the population studied here. These differences seem to be closely related to ecological characteristics of the environments inhabited by these populations. Our results supported the hypothesis that coastal and continental populations of M. amazonicum belong to the same species.

Morphology of the first larval stage of Macrobrachium brasiliense (Heller, 1868) (Caridea: Palaemonidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Kinetic Analysis of Gill (Na+,K+)-ATPase Activity in Selected Ontogenetic Stages of the Amazon River Shrimp, (Decapoda, Palaemonidae): Interactions at ATP- and Cation-Binding Sites

We investigated modulation by ATP, Mg2+, Na+, K+ and NH4 (+) and inhibition by ouabain of (Na+,K+)-ATPase activity in microsomal homogenates of whole zoeae I and decapodid III (formerly zoea IX) and whole-body and gill homogenates of juvenile and adult Amazon River shrimps, . (Na+,K+)-ATPase-specific activity was increased twofold in decapodid III compared to zoea I, juveniles and adults, suggesting an important role in this ontogenetic stage. The apparent affinity for ATP ( (M) = 0.09 +/- A 0.01 mmol L-1) of the decapodid III (Na+,K+)-ATPase, about twofold greater than the other stages, further highlights this relevance. Modulation of (Na+,K+)-ATPase activity by K+ also revealed a threefold greater affinity for K+ ( (0.5) = 0.91 +/- A 0.04 mmol L-1) in decapodid III than in other stages; NH4 (+) had no modulatory effect. The affinity for Na+ ( (0.5) = 13.2 +/- A 0.6 mmol L-1) of zoea I (Na+,K+)-ATPase was fourfold less than other stages. Modulation by Na+, Mg2+ and NH4 (+) obeyed cooperative kinetics, while K+ modulation exhibited Michaelis-Menten behavior. Rates of maximal Mg2+ stimulation of ouabain-insensitive ATPase activity differed in each ontogenetic stage, suggesting that Mg2+-stimulated ATPases other than (Na+,K+)-ATPase are present. Ouabain inhibition suggests that, among the various ATPase activities present in the different stages, Na+-ATPase may be involved in the ontogeny of osmoregulation in larval The NH4 (+)-stimulated, ouabain-insensitive ATPase activity seen in zoea I and decapodid III may reflect a stage-specific means of ammonia excretion since functional gills are absent in the early larval stages.

Content extraction: Identifying the main content in HTML documents

Except the article forming the main content most HTML documents on the WWW contain additional contents such as navigation menus, design elements or commercial banners. In the context of several applications it is necessary to draw the distinction between main and additional content automatically. Content extraction and template detection are the two approaches to solve this task. This thesis gives an extensive overview of existing algorithms from both areas. It contributes an objective way to measure and evaluate the performance of content extraction algorithms under different aspects. These evaluation measures allow to draw the first objective comparison of existing extraction solutions. The newly introduced content code blurring algorithm overcomes several drawbacks of previous approaches and proves to be the best content extraction algorithm at the moment. An analysis of methods to cluster web documents according to their underlying templates is the third major contribution of this thesis. In combination with a localised crawling process this clustering analysis can be used to automatically create sets of training documents for template detection algorithms. As the whole process can be automated it allows to perform template detection on a single document, thereby combining the advantages of single and multi document algorithms.

Demersal communities in the Mediterranean Sea: a case study of Triglidae (Osteichthyes, Scorpaeniformes) on the conservation and sustainable use of marine resources

An appropriate management of fisheries resources can only be achieved with the continuous supply of information on the structure and biology of populations, in order to predict the temporal fluctuations. This study supports the importance of investigating the bio-ecology of increasingly exploited and poorly known species, such as gurnards (Osteichthyes, Triglidae) from Adriatic Sea (Mediterranean), to quantify their ecological role into marine community. It also focuses on investigate inter and intra-specific structuring factor of Adriatic population. These objectives were achieved by: 1) investigating aspects of the population dynamics; 2) studying the feeding biology through the examination of stomach contents; 3) using sagittal otoliths as potential marker of species life cycle; 4) getting preliminary data on mDNA phylogeny. Gurnards showed a specie-specific “critical size” coinciding with the start of sexual maturity, the tendency to migrate to greater depths, a change of diet from crustaceans to fish and an increase of variety of food items eaten. Distribution of prey items, predator size range and depth distribution were the main dimensions that influence the breadth of trophic niche and the relative difference amongst Adriatic gurnards. Several feeding preferences were individuated and a possible impact among bigger-size gurnards and other commercial fishes (anchovy, gadoids) and Crustacea (such as mantis prawn and shrimps) were to be necessary considered. Otolith studies showed that gurnard species have a very fast growth despite other results in other areas; intra-specific differences and the increase in the variability of otolith shape, sulcus acusticus shape, S:O ratios, sulcus acusticus external crystals arrangement were shown between juveniles and adults and were linked to growth (individual genetic factors) and to environmental conditions (e.g. depth and trophic niche distribution). In order to facilitate correct biological interpretation of data, molecular data were obtained for comparing morphological distance to genetic ones.