Comparative studies of Vulva development in C. briggsae

As evolution progresses, developmental changes occur. Genes lose and gain molecular partners, regulatory sequences, and new functions. As a consequence, tissues evolve alternative methods to develop similar structures, more or less robust. How this occurs is a major question in biology. One method of addressing this question is by examining the developmental and genetic differences between similar species. Several studies of nematodes Pristionchus pacificus and Oscheius CEW1 have revealed various differences in vulval development from the well-studied C. elegans (e.g. gonad induction, competence group specification, and gene function.)

I approached the question of developmental change in a similar manner by using Caenorhabditis briggsae, a close relative of C. elegans. C. briggsae allows the use of transgenic approaches to determine developmental changes between species. We determined subtle changes in the competence group, in 1° cell specification, and vulval lineage.

We also analyzed the let-60 gene in four nematode species. We found conservation in the codon identity and exon-intron boundaries, but lack of an extended 3' untranslated region in Caenorhabditis briggsae.

Studies on hymenopteran parasitism of Drosophila

Studies on Hymenopteran Parasitism of Drosophila

Flies of the genus Drosophila are subject to attack by a number of parasitic forms. Sturtevant (1921) has listed records of parasitism by protozoa (Leptomonas), fungi (Muiaria and Stigmatomyces), nematodes, mites and v~rious hymenoptera. According to Sturtevant, Perkins (1913) has bred at least five species of hymenoptera, belonging to the proctotrupoid, cynipoid and chalcidoid groups, upon Drosophiline flies. H.S. Smith has bred an unidentified proctotrupoid and a chalcidoid, Pachy crepoideus dubius Ashmead* from both Drosophila melanogaster ani D. hydei. Kieffer ( 1913) has described three species of hymenoptera from Africa collected by Silvestri and stated by him to be parasitic on Drosophila, species not given. They are Trichopria (Planopria) rhopalica (Diapriidae), Ashmeadopria drosophilae (Diapriidae), and the insect which forms the subject matter of the present investigation, Eucoila drosophilae (Figitidae).

There are in addition a number of predacious enemies among wasps, spiders, flies and beetles.

The present account is concerned with parasitism of various species of Drosophila by Eucoila drosophilae Kieff. The wasps were found b y Dr. w. P. Spencer who exposed traps in an effort to collect Drosophila at Long Lake, Ohio, in Sept. 1934 . Drosophila larvae from the trap gave a large number of pupae from which wasps emerged in considerable proportions. Since that time stock s have been maintained in culture on Drosophila melanogaster.

Prevalence, intensity, and effect of a nematode (Philometra saltatrix) in the ovaries of bluefish (Pomatomus saltatrix)

Examination of 203 adult bluefish (Pomatomus saltatrix) from Long Island, New York, in 2002 and 2003 and 66 from the Outer Banks, North Carolina, in 2003 revealed the presence of dracunculoid nematodes (Philometra saltatrix) in the ovaries of female fish. Percent prevalence reached 88% in July and then decreased after the peak of the spawning season. Bluefish contained up to 100 parasites per fish. Infection was associated with a range of disorders, including hemorrhage, inf lammation, edema, prenecrotic and necrotic changes, and follicular atresia, that may prevent proper development of oocytes and probably affect bluefish fecundity. Historical occurrences, life cycle, and geographical distribution of this nematode remain largely unknown, but may play important roles in recruitment processes of bluefish.

The use of parasites in discriminating stocks of Pacific halibut (Hippoglossus stenolepis) in the northeast Pacific

The use of parasites as indicators of the stock structure of Pacific halibut (Hippoglossus stenolepis) in the northeast Pacific was investigated by using 328 adult (>55 cm fork length) halibut from 15 composite localities ranging from northern California to the northern Bering Sea and 96 juvenile (10–55 cm) halibut from five localities ranging from the northern Queen Charlotte Islands to the Bering Sea. Counts of eight selected parasite species (the juvenile acanthocephalans Corynosoma strumosum and C. villosum, the metacestode Nybelinia surmenicola, the digenean metacercaria Otodistomum sp., and the larval nematodes Anisakis simplex, Pseudoterranova decipiens, Contracaecum sp., and Spirurid gen. sp.) that produce infections of long duration, do not multiply in the host, and that have a relatively high abundance in at least one geographic locality were subjected to discriminant function analysis. Juvenile Pacific halibut showed no separation and, even though they were not heavily infected with parasites, the analysis suggested that juveniles could be a mixed stock. Three groups of adults were identified: fish from California to the southern Queen Charlotte Islands, those from the northern Queen Charlotte Islands to the central Bering Sea, and those from the central and north-ern Bering Sea. These groups suggest that the single stock concept be more thoroughly evaluated.

大功率微波与臭氧等复合因子对大棚蔬菜根结线虫及土壤的作

虫（nematodes）是世界农业生产的一大障碍，每年给世界农业生产造成约1000亿美元的巨额损失，是世界各国一直关注的重点病害之一。线虫可以危害各种大田作物和各种温室植物，几乎所有的栽培植物都有线虫危害发生。随着人们环保意识的增强以及健康意识的觉醒，传统防治根结线虫的药物如溴甲烷等因对环境的破坏作用，或者由于高毒、使用不便、抗药性等原因而迅速退出历史舞台，研究环保高效防治线虫成为世界各国竞相发展的技术之一。本研究尝试通过物理方式防治蔬菜根结线虫，并调查了对土壤质量的影响，主要内容包括： 1. 设计制造了功率20 Kw，频率为915 MHz的大功率土壤微波处理机，并通过了田间试验; 2. 设计制造了每小时120 g臭氧产量的便携式臭氧土壤处理机，并通过了田间试验; 3. 采用4因素3水平正交法L9(34)在山东寿光蔬菜大棚进行了蔬菜根结线虫的防治试验，综合考察微波、臭氧、微波吸收剂、EM菌等不同处理水平对根结线虫和对土壤质量的影响，结果表明： 1) 大棚土壤经微波60 s照射能够显著降低甜瓜根结线虫数和根结指数（p < 0.05），盆栽试验只需处理10 s即可显著防治甜瓜根结线虫（p < 0.01）;以120 g/h臭氧浓度处理5 s也能显著降低甜瓜根结线虫的数量（p < 0.05）;田间施入5 g/m2的EM菌，能够显著降低甜瓜根结线虫的数量（p < 0.05）;盆栽试验中微波吸收剂具有降低根结线虫数量的趋势，但是不同处理水平之间差异不显著，田间试验则具有增加根结线虫的趋势，不同处理水平之间也没有显著差异。 2) 微波不同处理水平之间对甜瓜单瓜重的影响没有显著差异;以120 g/h臭氧浓度处理甜瓜能够显著增加甜瓜单瓜重（p < 0.05）;200 g/m2微波吸收剂能够显著提高甜瓜单瓜重（p < 0.05）;以5 g/m2EM菌处理土壤则显著降低甜瓜单瓜重（p < 0.05）。 3) 微波、臭氧、微波吸收剂不同处理水平对甜瓜糖度的影响没有显著差异;以3 g/m2的EM菌处理甜瓜能够显著降低甜瓜的糖度。 4) 不同因素对甜瓜硬度的影响没有显著差异。 5) 微波处理60 s能够显著提高甜瓜的果型指数（p < 0.1），臭氧、微波吸收剂、EM菌等因素不同处理水平之间对果型指数影响差异不显著。 6) EM菌5 g/m2处理浓度能够显著降低甜瓜株高（p < 0.1），微波、臭氧、微波吸收剂不同处理水平之间对株高的影响没有显著差异。 7) EM菌5 g/m2处理浓度能够显著降低甜瓜根径（p < 0.1），微波、臭氧、微波吸收剂不同处理水平之间对根径的影响没有显著差异。 8) 微波处理60 s能够显著降低表层土壤（0-5 cm）的有机质含量（p < 0.01）;以120 g/h的臭氧浓度处理，则可以显著提高10-15 cm土壤的有机质含量（p < 0.05），其它处理因素的不同水平对有机质的影响差异不显著。 9) 微波不同处理水平对土壤全氮的影响没有显著差异（p < 0.05）;120 g/h臭氧处理浓度能够显著降低表层土壤（0-5 cm）的土壤全氮量（p < 0.1）;100 g/m2微波吸收剂处理水平能够显著提高10-15 cm土壤全氮量（p < 0.05）;EM菌3 g/m2的处理水平则显著降低5-10 cm土壤全氮量（p < 0.05）。 10) 微波、微波吸收剂、EM菌不同处理水平之间对土壤全磷没有显著影响;240 g/h臭氧浓度处理5 s能够显著降低10-15 cm土层的土壤全磷含量(p < 0.05)。 11) 微波、微波吸收剂对土壤全磷没有显著影响;臭氧处理具有降低土壤有效磷的趋势，EM菌处理则有提高土壤有效磷的趋势，但是 不同处理水平之间差异不显著（p > 0.05）。 12) 微波处理对土壤EC值没有显著影响;臭氧、微波吸收剂、EM菌处理具有降低土壤EC值的趋势，但是不同处理水平之间差异不显著（p > 0.05）。 13) 微波处理30 s能够显著降低表层土壤的pH值（p < 0.05），臭氧、微波吸收剂、EM菌处理具有提高土壤pH值的趋势，但是不同处理水平之间差异不显著。

A Preliminary survey of 21 Ceylon lakes. 3. Parasites and predators, food of fish, and marginal fauna

Three nematodes, two acanthocephalans and three cestodes were collected by dissecting 15 species of fish. Of these parasites only Hedruris sp. a larval nematode is responsible directly for the death of fish, since it is found in predatory species a reduction of these might prove of considerable consequence to fish, production. Bothriocephalus gowleonensis, a cestode probably introduced from China with Chinese carp, was recorded in an indigenous carp Puntius sarana. This parasite causes heavy mortality in grass carp in China and is a potential danger to local species. A single crustacean parasite was recorded. It is likely that they are widespread. No protozoa were recorded but there can be little doubt that they occur in local freshwater species and may be of considerable importance in fisheries. Several predators (insects, fish and other vertebrates) were recorded and are doubtless of considerable importance. However, the role of parasites and of predators (especially fishes) in relation to the productivity of fisheries can hardly be assessed at present and needs further study. A study of the fauna found at the shallow edges of 21 irrigation reservoirs showed an abundance of fauna in the low-country reservoirs whilst the up-country reservoirs had a poor fauna. The fauna showed insects, crustacea and mollusca in this order of abundance. The presence of insect and molluscs feeding fish in our fauna shows that these rich sources of food are being utilised.

Benthic diversity of River Gomti in relation to the prevailing environmental conditions in Lucknow

To evaluate the benthic diversity of River Gomti in relation to the prevailing environmental conditions, four stations, viz., Maa Chandrika Devi, Daliganj, Ambedkar Park and Aquaduct, were identified from upstream to downstream along the course of the river in Lucknow. Dissolved oxygen was low on many occasions at all the stations except Maa Chandrika Devi and chemical oxygen demand values were high. There was a gradual increase in mean nitrite and phosphate values from up to downstream. Benthic fauna was dominated by oligochaetes and chironomus larvae. Other groups reported were leeches, nematodes, sponges, crustaceans, pelycypodes, gastropods and fish fry. Population density was exceptionally high at Daliganj (20,135 m sub(-1) ) followed by Ambedkar Park (5,199 m sub(-1)) and Aquaduct (3,287 m sub(-1)), and low at Maa Chandrika Devi (264 m sub(-1)). Oligochaete genera common at all the four stations were Lumbricillus, Limnodrillus, Branchiura, Chaetogaster, Nais and Tubifex. Odonates were reported only from Maa Chandrika Devi while sponges were encountered at Daliganj and Aquaduct. On some occasions, fish fry were also found at Ambedkar Park and Aquaduct. Seasonally, maxima for population density were observed during pre-monsoon and minima during monsoon. The organic pollution indicator benthic species reported were tubificids, chironomids, culicoid larvae, Lamellidens sp., Corbicula sp., Lymnaea sp. and leech. Branchiurans, Tubifex sp. and Chironomus larvae were reported at all the stations. Filthy condition with foul smell throughout the length of the river coupled with poor water quality and appearance of indicator organisms at all the stations indicate that the river is under severe pollution stress due to anthropogenic discharges and it has reached an alarming stage.

Fish-makhana (Euryale salisb) integration: a case study of sustainable aquafarming system in north Bihar

The paper deals with a technique to synchronize two crops, fish and makhana (Euryale ferox Salisb) in a pond. In such eco-friendly integration both crops are mutually benefited. Decomposed plant parts of makhana crop form organic matter that releases nutrients in the water to enhance plankton population. Organic detritus not only acts as food for bottom dwelling fishes (mrigal and common carp) but also provides a suitable substratum for the growth of zooplankton, insect larvae, nematodes and gastropods. Fishes contribute to the control of makhana pests. Their faecal matter acts as organic manure for makhana crop. Plankton population fluctuated between 1260 u/l to 4030 u/l in the control pond and 1630 u/l to 4722 u/l in the experimental pond. During the grand growth period of makhana crop (April to July) the dissolved oxygen content fluctuated between 5.02 mg/l to 6.68 mg/l in the covered areas and 6.04 mg/l to 6.92 mg/l in uncovered areas. Makhana leaves acting as blanket barrier over the water surface brought down the D.O. content in the covered areas of the pond. Free CO sub(2) content showed wider fluctuation in the experimental pond (25.2 mg/l to 30.9 mg/l) than in the control pond (25.1 mg/l to 28.6 mg/l). This could be due to decomposition of plant parts of the presiding crop lying as debris at the pond bottom. Autochthonous supply of nutrients enhanced the content of nitrogen, phosphorous and organic carbon in the soil of experimental pond. The experimental pond covering an area of 0.40 ha yielded 852 kg fish and 200 kg pops whereas the control pond covering the same area produced 777 kg fish only. The net profit per ha came out to be Rs.1,04,700 and Rs. 66,200 in integrated and non-integrated system respectively. Owing to crop diversification, the present integrated system was found to be more viable than the non-integrated system in terms of production and net profit.

Abundance and composition of benthic fauna in Penaeus monodon Fabricius culture pond on the west coast of Malaysia peninsular

This paper reports a study on the benthic faunal abundance and diversity of tiger shrimp P. monodon culture ponds in Perak, west coast of Malaysia Peninsular. Sampling was carried out at three weeks interval throughout the 116 days culture period. In addition, water temperature, dissolved oxygen, salinity, transparency, pH and organic matter of soil were also measured. Results showed that the major groups of macro-benthos comprised of gastropod, foraminifera, polychaetes, bivalve and insects; whereas the meio-benthos comprised of harpacticoid copepods, ostracods, nematodes, gastropods, foraminifera, bivalve, insects, crustacean nauplii and polychaetes. In macro-benthos, the abundance of different sizes of Gastropods increased throughout the culture duration. This consisted of 37-98.20% for <1cm length, 1.80-61.50% for 1-2cm length and 1.18—1.30% for >2cm length. Other macro and meio-benthic organisms decreased linearly with the culture period. The depletion symptom indicates that the culture species may have intensively preyed upon the consumable (<0.5cm in size) benthic fauna together with detritus and artificial diet; or could have been caused by pond bottom deterioration via uneaten feed, faces and toxic gases.

A note on some parasites of Merluccius capensis (Pisces) and their zoogeographical significance

During the Southeastern Atlantic Expedition of the German fishery research vessel "Walther Herwig" in 1967 the main emphasis lay on selective fishing of the South African hake Merluccius capensis (von BRANDT 1967). Some of the fish were found to be infested by ecto-and endoparasites both of which were collected whenever possible. Large plerocercoids of Dibothriorhynchus grossum whose adult stage lives in the South Atlantic Ocean in Lamna cornubica (L.SZIDAT, personal communication) were quite common as were cysticercoids of a Tetrarhynchus sp., which had also been reported in Cynoscion striatus off the Argentinian coast (MACDONAGH 1927, cited in Szidat, personal communication). Brownish nematodes were infesting the ovaries of several fish, but could not be identified. The most common ectoparasite to be observed was the parasitic isopod Livoneca raynaudii (fam. Cymothoidae) whose early larval stages were also found.

Molecular characteristics of Camallanus spp. (Spirurida : Camallanidae) in fishes from China based on its rDNA sequences

In the,paper, we explored the intra- and interspecific evolutionary variation among species of Camallanus collected from different fish species in various regions of China. We determined the internal transcribed spacers of ribosomal DNA (ITS rDNA) sequences of these nematodes. The divergence (uncorrected p-distance) of ITS 1, ITS2, and ITS rDNA data sets confirmed 2 valid species of Camallanus in China, i.e., C. cotti and C. hypophthalmichthys. The 2 species were distinguished not only by their different morphologies and host ranges but also by a letranucleotide microsatellite (TTGC)n present in the ITS I region of C cotti. Phylogenetic analyses of the nematodes disclosed 2 main clades, corresponding to different individuals of C cotti and C. hypophthalmichthys from different fish species in various geographical locations, although the interior nodes of each clade received poor support.

Population dynamics and maturation cycle of Camallanus cotti (Nematoda : Camallanidae) in the Chinese hooksnout carp Opsariichthys bidens (Osteichthyes : Cyprinidae) from a reservoir in China

The seasonal population dynamics and maturation cycle of the nematode Camallanus cotti in the posterior intestine of Chinese hooksnout carp Opsariichthys bidens have been studied in the Danjiangkou Reservoir of the Hubei Province in central China from September 2004 to November, 2005. The overall prevalence, mean abundance and intensity of C cotti among fish sampled (n = 700 fish) were 47%, 2.29 +/- 12.38 ( +/- S.D.) and 1-307 (average 4.89 +/- 17.74), respectively. The overall sexual ratio of female to male nematodes (excluding L3 and L4 juveniles) was 1.17:1. Statistical results showed weakly positive correlations betweerl fish length and the number of nematodes per host. The dynamics of infection of the nematode exhibited significant seasonal pattern in changes in mean abundance. A similar pattern was found for changes in nematode prevalence, although this was not statistically significant. Higher levels of infection were observed among fish sampled in summer months and the lower in the winter. Neither the prevalence nor the abundance of the parasite was significantly different between male and female hosts. The pattern of frequency distribution of the parasite in the host was found to be over-dispersed throughout the sampling period. In addition, studies on the development and maturation of the parasite in O. bidens revealed that development (maturation), recruitment of the next generation, and reproduction may be continuous year-round, although reproduction may peak during the winter. (c) 2007 Elsevier B.V. All rights reserved.

New data on the morphology and systematic status of Spinitectus petrowi and Spinitectus gigi (Nematoda : Cystidicolidae) parasitic in catfishes in central China

Two little-known nematode species of the genus Spinitectus Fourment, 1883, S. petrowi Belous, 1965 (prevalence 25%, intensity 1-8) and S. gigi Fujita, 1927 (prevalence 10%, intensity 2-3), were collected from the gastrointestinal tract of the yellow catfish, Pelteobagrus fulvidraco (Richardson), from Liangzihu Lake, Hubei Province, central China, in September of 2002. The light and scanning electron microscopical examination of this material, supplemented by a few museum specimens of S. gigi collected from the catfish Clarias fuscus (Lacepede) in southern China, made it possible to study in detail the morphology of these parasite species and to redescribe them. The first species, whose correct name is S. petrowi Belous, 1965, exhibits some morphological features (e.g., unusually short vestibule, shape of pseudolabia and of the left spicule) not found in most other congeners; a unique feature is the presence of peculiar pairs of transversely oriented peg-like cuticular spines with rounded ends on the ventral surface of the female tail. Spinitectus gigi was found to have 28-31 cuticular spines in the first ring, relatively long distances between the 2nd-7th rings of spines, and anterior rings divided into 2 sectors; the excretory pore is located at the level of the 4th ring of cuticular spines; males posses 4 pairs of preanal- and 6 pairs of postanal caudal papillae and a pair of small phasmids. Spinitectus bagri Wang, Wu et Yu, 1993 and S. wulingensis Yu et Wang, 1997 are considered junior synonyms of S. petrowi, whereas S. clariasi Ky, 1971, S. ophicephali Ky, 1971 and S. yuanjiangensis Wang, Wit et Yu, 1997 are regarded to be junior synonyms of S. gigi. Spinitectus petrowi was not previously reported from China.

Distribution of the meiofaunal community in a eutrophic shallow lake of China

In a Chinese eutrophic shallow lake, the spatial, temporal and vertical distributions of meiofauna in different lake zones along a eutrophic gradient were analyzed. The spatial distribution of meiofauna among sampling stations changed with nutrient levels. Nematoda were most abundant at the majority of sampling stations comprising 70.6 - 93.2 % meiofaunal abundance except for a hypereutrophic station. The seasonal patterns in abundance of nematodes, oligochaetes, rotifers, chironomids and different nematode feeding groups differed among stations, which revealed that the temporal variations of these meiofaunal groups and the nematode feeding groups may vary with different nutrient loadings. The vertical distributions of meiofaunal groups, nematode species, and nematode trophic groups in the upper and lower sediment layers were similar, suggesting a consistent vertical distribution pattern across different trophic conditions. Nematode species richness, Shannon-Wiener species diversity index, trophic diversity and Maturity Index were significantly correlated with nutrient levels (total phosphorus and nitrogen in lake water and total phosphorus in sediment). Our results suggest the importance of nematode community analyses in the assessment of freshwater eutrophication.

CO2浓度升高和氮肥管理对土壤线虫群落的影响

大气CO2浓度升高能够对农田生态系统产生一系列的影响。土壤线虫在农田生态系统腐屑食物网中占有重要的地位，能够对外界环境变化作出较迅速的响应。本文利用江苏省江都市小记镇的稻-麦轮作FACE系统研究平台，在2007-2008年小麦生长季，研究了大气CO2浓度升高和不同氮肥处理（高N和低N）对农田土壤线虫群落的影响。 研究结果表明：高氮肥施用情况下, CO2浓度升高显著降低了麦田土壤铵态氮和硝态氮含量。不同氮肥处理中CO2浓度升高条件下土壤可溶性碳的含量显著低于对照，而土壤总有机碳和微生物量碳含量高于对照。 大气CO2浓度升高条件下，麦田土壤线虫群落组成和多样性与对照相比表现出显著差异。CO2浓度升高显著增加了麦田土壤线虫总数、食细菌线虫、食真菌线虫和植物寄生线虫数量。在小麦拔节期和成熟期，低N和高N施用条件下，FACE处理中土壤线虫多样性指数（H’）、成熟度指数（MI和PPI）均低于对照处理，而结构指数（SI）高于对照处理。线虫生态指数的结果表明，大气CO2浓度升高条件下，土壤线虫群落多样性降低，土壤环境受到一定的干扰，食物网趋于结构化。