Contemporary genetic, historical genetic and morphological data sets for the Yellow-tufted Honeyeater Lichenostomus melanops

Understanding the evolutionary history of threatened populations can improve their conservation management. Re-establishment of past but recent gene flow could re-invigorate threatened populations and replenish genetic diversity, necessary for population persistence. One of the four nominal subspecies of the common yellow-tufted honeyeater, Lichenostomus melanops cassidix, is critically endangered despite substantial conservation efforts over 55 years. Using a combination of morphometric, genetic and modelling approaches we tested for its evolutionary distinctiveness and conservation merit. We confirmed that cassidix has at least one morphometric distinction. It also differs genetically from the other subspecies in allele frequencies but not phylogenetically, implying that its evolution was recent. Modelling historical distribution supported the lack of vicariance and suggested a possibility of gene flow among subspecies at least since the late Pleistocene. Multi-locus coalescent analyses indicated that cassidix diverged from its common ancestor with neighbouring subspecies gippslandicus sometime from the mid-Pleistocene to the Holocene, and that it has the smallest historical effective population size of all subspecies. It appears that cassidix diverged from its ancestor with gippslandicus through a combination of drift and local selection. From patterns of genetic subdivision on two spatial scales and morphological variation we concluded that cassidix, gippslandicus and (melanops + meltoni) are diagnosable as subspecies. Low genetic diversity and effective population size of cassidix may translate to low genetic fitness and evolutionary potential, thus managed gene flow from gippslandicus is recommended for its recovery.

Seawater carbonate chemistry and early development of the sea urchin Lytechinus variegatus in a laboratory experiment using artificial seawater

Land-based aquaculture facilities often utilize additional bicarbonate sources such as commercial sea salts that are designed to boost alkalinity in order to buffer seawater against reductions in pH. Despite these preventative measures, many facilities are likely to face occasional reductions in pH and corresponding reductions in carbonate saturation states due to the accumulation of metabolic waste products. We investigated the impact of reduced carbonate saturation states (Omega Ca, Omega Ar) on embryonic developmental rates, larval developmental rates, and echinoplutei skeletal morphometrics in the common edible sea urchin Lytechinus variegatus under high alkalinity conditions. Commercial artificial seawater was bubbled with a mixture of air and CO2 gas to reduce the carbonate saturation state. Rates of embryonic and larval development were significantly delayed in both the low and extreme low carbonate saturation state groups relative to the control at a given time. Although symmetry of overall skeletal body lengths was not affected, allometric relationships were significantly different between treatment groups. Larvae reared under ambient conditions had significantly greater postoral arm and overall body lengths relative to body lengths than larvae grown under extreme low carbonate saturation state conditions, indicating that extreme changes in the carbonate system affected not only developmental rates but also larval skeletal shape. Reduced rates of embryonic development and delayed and altered larval skeletal growth are likely to negatively impact larval culturing of L. variegatus in land-based, intensive culture situations where calcite and aragonite saturation states are lowered by the accumulation of metabolic waste products.

Seawater carbonate chemistry, mortality and hatching rate, size and mass of Clupea harengus during experiments, 2011

Due to atmospheric accumulation of anthropogenic CO2 the partial pressure of carbon dioxide (pCO2) in surface seawater increases and the pH decreases. This process known as ocean acidification might have severe effects on marine organisms and ecosystems. The present study addresses the effect of ocean acidification on early developmental stages, the most sensitive stages in life history, of the Atlantic herring (Clupea harengus L.). Eggs of the Atlantic herring were fertilized and incubated in artificially acidified seawater (pCO2 1260, 1859, 2626, 2903, 4635 µatm) and a control treatment (pCO2 480 µatm) until the main hatch of herring larvae occurred. The development of the embryos was monitored daily and newly hatched larvae were sampled to analyze their morphometrics, and their condition by measuring the RNA/DNA ratios. Elevated pCO2 neither affected the embryogenesis nor the hatch rate. Furthermore the results showed no linear relationship betweenpCO2 and total length, dry weight, yolk sac area and otolith area of the newly hatched larvae. For pCO2 and RNA/DNA ratio, however, a significant negative linear relationship was found. The RNA concentration at hatching was reduced at higher pCO2 levels, which could lead to a decreased protein biosynthesis. The results indicate that an increased pCO2 can affect the metabolism of herring embryos negatively. Accordingly, further somatic growth of the larvae could be reduced. This can have consequences for the larval fish, since smaller and slow growing individuals have a lower survival potential due to lower feeding success and increased predation mortality. The regulatory mechanisms necessary to compensate for effects of hypercapnia could therefore lead to lower larval survival. Since the recruitment of fish seems to be determined during the early life stages, future research on the factors influencing these stages are of great importance in fisheries science.

Seismic Data from MSM21/4 to Knipovich Ridge on the western Svalbard margin

The seismic data were acquired north of the Knipovich Ridge on the western Svalbard margin during cruise MSM21/4. They were recorded using a Geometrics GeoEel streamer of either 120 channels (profiles p100-p208) or 88 channels (profiles p300-p805) with a group spacing of 1.56 m and a sampling rate of 2 kHz. A GI-Gun (2×1.7 l) with a main frequency of ~150 Hz was used as a source and operated at a shot interval of 6-8 s. Processing of profiles p100-p208 and p600-p805: Positions for each channel were calculated by backtracking along the profiles from the GI-Gun GPS positions. The shot gathers were analyzed for abnormal amplitudes below the seafloor reflection by comparing neighboring traces in different frequency bands within sliding time windows. To suppress surface-generated water noise, a tau-p filter was applied in the shot gather domain. Common mid-point (CMP) profiles were then generated through crooked-line binning with a CMP spacing of 1.5625 m. A zero-phase band-pass filter with corner frequencies of 60 Hz and 360 Hz was applied to the data. Based on regional velocity information from MCS data [Sarkar, 2012], an interpolated and extrapolated 3D interval velocity model was created below the digitized seafloor reflection of the high-resolution streamer data. This velocity model was used to apply a CMP stack and an amplitude-preserving Kirchhoff post-stack time migration. Processing of profiles p400-p500: Data were sampled at 0.5 ms and sorted into common midpoint (CMP) domain with a bin spacing of 5 m. Normal move out correction was carried out with a velocity of 1500 m s-1 and an Ormsby bandpass filter with corner frequencies at 40, 80, 600 and 1000 Hz was applied. The data were time migrated using the water velocity.

El Problema de Lambert en el Contexto de Optimización de Trayectorias Espaciales

Esta tesis se basa en el estudio de la trayectoria que pasa por dos puntos en el problema de los dos cuerpos, inicialmente desarrollado por Lambert, del que toma su nombre. En el pasado, el Problema de Lambert se ha utilizado para la determinación de órbitas a partir de observaciones astronómicas de los cuerpos celestes. Actualmente, se utiliza continuamente en determinación de órbitas, misiones planetaria e interplanetarias, encuentro espacial e interceptación, o incluso en corrección de orbitas. Dada su gran importancia, se decide investigar especialmente sobre su solución y las aplicaciones en las misiones espaciales actuales. El campo de investigación abierto, es muy amplio, así que, es necesario determinar unos objetivos específicos realistas, en el contexto de ejecución de una Tesis, pero que sirvan para mostrar con suficiente claridad el potencial de los resultados aportados en este trabajo, e incluso poder extenderlos a otros campos de aplicación. Como resultado de este análisis, el objetivo principal de la Tesis se enfoca en el desarrollo de algoritmos para resolver el Problema de Lambert, que puedan ser aplicados de forma muy eficiente en las misiones reales donde aparece. En todos los desarrollos, se ha considerado especialmente la eficiencia del cálculo computacional necesario en comparación con los métodos existentes en la actualidad, destacando la forma de evitar la pérdida de precisión inherente a este tipo de algoritmos y la posibilidad de aplicar cualquier método iterativo que implique el uso de derivadas de cualquier orden. En busca de estos objetivos, se desarrollan varias soluciones para resolver el Problema de Lambert, todas ellas basadas en la resolución de ecuaciones transcendentes, con las cuales, se alcanzan las siguientes aportaciones principales de este trabajo: • Una forma genérica completamente diferente de obtener las diversas ecuaciones para resolver el Problema de Lambert, mediante desarrollo analítico, desde cero, a partir de las ecuaciones elementales conocidas de las cónicas (geométricas y temporal), proporcionando en todas ellas fórmulas para el cálculo de derivadas de cualquier orden. • Proporcionar una visión unificada de las ecuaciones más relevantes existentes, mostrando la equivalencia con variantes de las ecuaciones aquí desarrolladas. • Deducción de una nueva variante de ecuación, el mayor logro de esta Tesis, que destaca en eficiencia sobre todas las demás (tanto en coste como en precisión). • Estudio de la sensibilidad de la solución ante variación de los datos iniciales, y como aplicar los resultados a casos reales de optimización de trayectorias. • También, a partir de los resultados, es posible deducir muchas propiedades utilizadas en la literatura para simplificar el problema, en particular la propiedad de invariancia, que conduce al Problema Transformado Simplificado. ABSTRACT This thesis is based on the study of the two-body, two-point boundary-value problem, initially developed by Lambert, from who it takes its name. Since the past, Lambert's Problem has been used for orbit determination from astronomical observations of celestial bodies. Currently, it is continuously used in orbit determinations, for planetary and interplanetary missions, space rendezvous, and interception, or even in orbit corrections. Given its great importance, it is decided to investigate their solution and applications in the current space missions. The open research field is very wide, it is necessary to determine specific and realistic objectives in the execution context of a Thesis, but that these serve to show clearly enough the potential of the results provided in this work, and even to extended them to other areas of application. As a result of this analysis, the main aim of the thesis focuses on the development of algorithms to solve the Lambert’s Problem which can be applied very efficiently in real missions where it appears. In all these developments, it has been specially considered the efficiency of the required computational calculation compared to currently existing methods, highlighting how to avoid the loss of precision inherent in such algorithms and the possibility to apply any iterative method involving the use of derivatives of any order. Looking to meet these objectives, a number of solutions to solve the Lambert’s Problem are developed, all based on the resolution of transcendental equations, with which the following main contributions of this work are reached: • A completely different generic way to get the various equations to solve the Lambert’s Problem by analytical development, from scratch, from the known elementary conic equations (geometrics and temporal), by providing, in all cases, the calculation of derivatives of any order. • Provide a unified view of most existing relevant equations, showing the equivalence with variants of the equations developed here. • Deduction of a new variant of equation, the goal of this Thesis, which emphasizes efficiency (both computational cost and accuracy) over all other. • Estudio de la sensibilidad de la solución ante la variación de las condiciones iniciales, mostrando cómo aprovechar los resultados a casos reales de optimización de trayectorias. • Study of the sensitivity of the solution to the variation of the initial data, and how to use the results to real cases of trajectories’ optimization. • Additionally, from results, it is possible to deduce many properties used in literature to simplify the problem, in particular the invariance property, which leads to a simplified transformed problem.

Biología reproductiva de Polioptila lembeyei (Aves: Polioptilidae) en la Reserva Ecológica Siboney-Juticí, Santiago de Cuba, Cuba

Se estudió la biología reproductiva de Polioptila lembeyei en la Reserva Ecológica Siboney-Juticí, Santiago de Cuba, Cuba durante los años 2006 y 2007 en seis formaciones vegetales. Se describieron aspectos de su reproducción en términos de cronología reproductiva, tamaño de puesta, duración del período de incubación, permanencia de los polluelos en el nido, morfometría de nidos y huevos, y caracterización del sitio de nidificación en cuanto a la altura de los nidos y especie y altura de las plantas utilizadas como sitio de nidificación. Se localizaron 89 nidos durante las dos temporadas reproductivas, 43 en el año 2006 y 46 en el 2007. El período reproductivo se extendió desde finales de marzo hasta julio, abarcando 129 días en el año 2006 y 122 días durante el año 2007. Las nidadas tuvieron una duración de 36–56 días (construcción 8,6 ± 2,8 días, puesta 8,6 ± 2,7 días, incubación de 14,0 ± 1,2 días y permanencia de los pichones en el nido 14,5 ± 1,0 días). De los 45 nidos examinados el tamaño de puesta modal fue de tres huevos (88,9 %), encontrándose también nidadas de dos (6,7 %) y cuatro huevos (4,4 %). De 38 nidos con huevos para ambas temporadas, 15 nidos produjeron pichones (32 pichones en el 2006 y 20 pichones en el 2007) y solo tres nidos durante el 2006 (37,5 %) lograron producir volantones, los cuales salieron con éxito del nido. La planta más utilizada como sitio de nidificación fue Acacia macracantha (80 % de los nidos detectados), aunque también se hallaron nidos en otras ocho especies de plantas. Las alturas de ubicación del nido fue de 2,7 ± 1,5 m (n = 82; rango 0,3–6,4 m) y la distancia del nido al dosel de la planta utilizada como sustrato fue de 1,1 ± 0,9 m (n = 80; rango 0,1–5,4 m).

Statistical models of accidents on interchange ramps and speed-change lanes.

Federal Highway Administration, Office of Safety and Traffic Operations, Washington, D.C.

Rolling resistance of tires measured under transient and equilibrium conditions on Calspan's tire research. Final report.

Transportation Department, Office of the Assistant Secretary for Systems Development and Technology, Washington, D.C.

No-passing zone treatments for special geometric and traffic operational situations. Final report.

Federal Highway Administration, Traffic Systems Division, Washington, D.C.

Highway geometric design consistency related to driver expectancy. Volume I: executive summary. Final report.

Federal Highway Administration, Environmental Division, Washington, D.C.

Highway geometric design consistency related to driver expectancy. Volume II: research report. Final report.

Federal Highway Administration, Environmental Division, Washington, D.C.