The environmental dimension in development planning

Includes bibliography

Institutional organization of the State for incorporating the environmental dimension in development planning

Includes bibliography

The legal aspects of the incorporation of the environmental dimension in development planning

Includes bibliography

Environmental protection measures, their evaluation (cost-benefit analysis) and their incorporation in development planning

Includes bibliography

The Mexican film industry and its participation in the global value chain

Includes bibliography

Private participation in the provision of water services: alternative means for private participation in the provision of water services

Includes bibliography

Participation in the context of the world economy

Includes bibliography

Worker participation in company profits or operating results in Latin America

Includes bibliography

Descentralization and participation in Latin America: an economic perspective

Includes bibliography

The limits of the possible in regional planning

Includes bibliography

The natural absence of RPA1N domain did not impair Leishmania amazonensis RPA-1 participation in DNA damage response and telomere protection.

We have previously shown that the subunit 1 of Leishmania amazonensis RPA (LaRPA-1) alone binds the G-rich telomeric strand and is structurally different from other RPA-1. It is analogous to telomere end-binding proteins described in model eukaryotes whose homologues were not identified in the protozoan's genome. Here we show that LaRPA-1 is involved with damage response and telomere protection although it lacks the RPA1N domain involved with the binding with multiple checkpoint proteins. We induced DNA double-strand breaks (DSBs) in Leishmania using phleomycin. Damage was confirmed by TUNEL-positive nuclei and triggered a G1/S cell cycle arrest that was accompanied by nuclear accumulation of LaRPA-1 and RAD51 in the S phase of hydroxyurea-synchronized parasites. DSBs also increased the levels of RAD51 in non-synchronized parasites and of LaRPA-1 and RAD51 in the S phase of synchronized cells. More LaRPA-1 appeared immunoprecipitating telomeres in vivo and associated in a complex containing RAD51, although this interaction needs more investigation. RAD51 apparently co-localized with few telomeric clusters but it did not immunoprecipitate telomeric DNA. These findings suggest that LaRPA-1 and RAD51 work together in response to DNA DSBs and at telomeres, upon damage, LaRPA-1 works probably to prevent loss of single-stranded DNA and to assume a capping function.

Acceso al poder y participación en la toma de decisiones en América Latina y el Caribe: políticas de equidad de género hacia el año 2000 = Access to power and participation in decision-making in Latin America and the Caribbean: policies for gender equity looking to the year 2000

Incluye Bibliografía

Report of the First Meeting of the Ad Hoc Working Group in Transportation Planning

Informe de la reunion del Grupo de Trabajo sobre Planificacion del Transporte cuyo debate se centro en las prioridades para el futuro programa de trabajo del Grupo. Incluye lista de participantes y de documentos.

Report of the First Meeting of the Ad Hoc Working Group in Agricultural Planning

Informa sobre la reunion que tuvo por objetivo la formulacion de estrategias de desarrollo para el sector agricola en la region.