Fibre analysis and fibre digestibility in pet foods - a comparison of total dietary fibre, neutral and acid detergent fibre and crude fibre

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of Phytase Supplementation in Diets on Nutrient Digestibility and Performance in Broiler Chicks

A performance trial was conducted with broiler chicks to Study the effect of phytase (PHY) supplementation in diets formulated With reduced AME, Ca, and P. The nutrient digestibility was determined during the 14- to 21-d and 28- to 35-d periods. The treatments consisted of 3 diets (NC1, NC2. NC3) differing ill nutrient content and each diet with Or without supplemental PHY (NC1, 0 oi 500; NC2, 0 or 750; NC3, 0 or 1,000 U of PHY/kg feed) and I positive control diet (PC). Compared with the PC diet. negative control diets (NC) resulted in lower AME and apparent ileal amino acid digestibility for some amino acids. Phytase Supplementation of the NC diets increased AME. apparent ileal amino acid digestibility, and apparent ileal crude protein digestibility. Phytase addition also increased mineral absorption in 2 1 - and 35-d-old broilers fed NC diets. Reduced nutrient digestibility appears to be I factor in the weight gain and feed intake results. Reducing Ca and P content reduced feed intake in a stepwise fashion in the NC diets. Phytase increased feed intake and generally improved nutrient digestibility, which resulted in an increase in digestible nutrient intake. Averaged across NC diets. PHY improved body weight. Bone-breaking strength was the most consistent predictor of Ca and P reduction. All NC diets had significantly lower bone-breaking strength than the PC. Phytase supplementation of the NC diets gave bone-breaking strengths that were comparable to the PC. Diets with PHY had the highest bioeconomic index.

Degradabilidade ruminal do amido de silagem de milho, farelo de soja e sorgo grão, em bovinos da raça Nelore

Four Bos indicus (Nellore) ruminally-fistulated animals, averaging 36 months of age and 520 kg, were used in a randomized block design to study the ruminal degradability of starch in corn silage, soybean meal, and sorghum grain. The experimental diets consisted of two levels of concentrate (18 and 39%) plus corn silage. The concentrate ingredients used were soybean meal, cottonseed meal, corn grain, and sorghum grain. The degradation rate of sorghum grain increased 40.2% in diet 2 . The potential degradability of starch (PDS) was similar in the two diets. There was no difference between the two diets in the effective degradability of starch (EDS) in corn silage. There was a significant increase of 26.5% in the EDS of starch in sorghum grain with the increase of level of concentrate in the diet, and considering the lag time in the calculation. The starch level estimated in this study was lower than the values cited in the literature, probably due to different laboratory methods for determining the starch content in feedstuffs. Previous studies suggested using a minimum of 6 incubation intervals between 2 and 24 hours.

Degradabilidade ruminal da matéria seca e da fração protéica da silagem de milho, do farelo de soja e do sorgo grão, em bovinos da raça nelore. Comparação com os dados obtidos pelo CNCPS

The nylon bag in situ degradation thecnique was employed to compare the data of the CNCPS to the ruminal degradability of the dry matter and crude protein in corn silage, soybean meal and sorghum grain, in four rumen fistulated Nellore steers, averaging 36 months of age and 520 kg of liveweight. A randomized complete block experimental design was used, where animals constituted the blocks. Two levels of concentrate, 18 and 39 %, were used in the diets. The forage used in the diets was corn silage and the concentrate ingredients were: soybean meal, cottonseed meal, corn grain and sorghum grain. There was a reduction in the potentially by degradable fraction (B) of the dry matter(DM) of the corn silage and sorghum grain with an increase in the concentrate level of the diet; however, the degradation rate(c) of the silage was similar for the two diets and the sorghum grain showed an increase of 28.4 %. The B fraction of the DM from the soybean meal was not affected by the diet, but its rate (c) was reduced by 18.1 %. The same effect was observed for the rate(c) of crude protein(CP) of the soybean meal, with a reduction of 38.1 %. The values for the effective degradability of the two fractions were not affected by the diet when the lag time was not considered. When lag time was considered, the degradability values of the studied feeds were superior in both fractions.

Estádio de desenvolvimento do milho. 3. Avaliação de silagens por intermédio de parâmetros de fermentação ruminal

The objective of this experiment was to analyze the rumen fermentation of silages made from corn harvested at milk stage (MS), milk early dough stage (MEDS), medium dough stage (MDS) and semi-hard dough stage (SHDS). Rumen fluid was collected from sheep by esophageal tube at 0, 1, 3 and 6 hours after feeding. There were no differences among silages for ammonia nitrogen (NH3-N) and methylene blue reduction time (MBRT). Only the MS and SHDS silages differed in rumen pH (6.82 and 6.53, respectively). Differences in total rumen VFA and acetic acid concentrations (mmoles/L) were observed among stages, but not between MS (36.40 and 22.13) and MEDS (42.49 and 25.73), nor between MDS (64.52 and 40.34 respectively) and SHDS (64.09 and 43.61, respectively). The periods of 1 and 3 hours after feeding showed the smallest pH values (6.47 and 6.63), the highest NH3-N concentrations (9.75 and 10.56 mg/dL) and the highest concentrations of total VFA, and acetic and propionic acids (60.33, 37.05 and 16.73; 59.40, 35.28 and 16.84 mmoles/L, respectively). On the whole, the MDS and SHDS silages showed the best rumen fermentation patterns based on pH and total and individual VFA values.

Aditivos na ensilagem do capim-elefante. IV. Fermentação ruminal em ovinos

The ruminai fermentation patterns of sheep fed elephant grass (Pennisetum purpureum Schum.) silage enriched with ground ear corn with husks, wheat bran and saccharin in the levels 0, 8, 16 and 24% dry weight of additive/wet weight of green chop was evaluated. A split-plot randomized block design was used. The plots were the additives and their levels and the sub-plots the time of rumen fluid collection (0, 1, 3, 6, 9, 12 and 24 h after feeding). During the collection period, the sheep were fed 80% of the observed voluntary feed intake of the previous phase. For all additive types and levels used in preparing the silages, high levels of total volatile fat acids were observed, with predominance of the acetic acid. The silages having ground ear corn with husks as additive showed, in the ruminai fluid, ammonia production levels below the recommended for maximum microbial protein synthesis. However, silages with saccharin or wheat bran presented a good ammoniacal-N availability. In the ruminal fluid of the sheep fed ground ear corn with husks or wheat bran the molar proportion of butyric acid was increased and that of acetic acid and pH were decreased, as the levels of the additives in the silage increased.

Desempenho e Digestibilidade in vivo de Cordeiros Alimentados com Dietas Contendo Canola em Grão Integral em Diferentes Formas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Acid and fermented silage characterization and determination of apparent digestibility coefficient of crude protein for pacu Piaractus mesopotamicus

Three types of raw materials including commercial waste from saltwater (SW), freshwater fish (FW) and tilapia fillet residue (FR) were used to produce fish silage by either acid digestion (2% formic acid and 2% sulfuric acid) or anaerobic fermentation (5% of Lactobacillus plantarum and 15% sugar cane molasses). Six test diets were used in digestibility trials prepared with 70% reference diet and 30% of each experimental silage. These diets were fed to juvenile pacu Piaractus mesopotamicus (146 g average weight) in triplicate. Fish were kept in 500-L tanks and feces collected by manual extrusion. It was observed for both processes that SW waste always had the highest moisture content and lowest fat and ash. Highest crude protein levels were found in silages from commercial fish waste (SW and FW) made from whole fish unfit for human consumption. However, apparent digestibility coefficients did not vary among diets (P > 0.05). Although values did not differ statistically, fermented silage consistently displayed higher digestibility coefficients compared to acid silage. The silages exhibited relatively high protein digestibility (72.5-80.0%), thus suggesting the feasibility of using fish industry by-products in aquaculture feeds.

Apparent digestible energy and nutrient digestibility coefficients of diet ingredients for pacu Piaractus brachypomus

The digestible energy and apparent nutrient digestibility coefficients of common diet ingredients were determined for pacu Piaractus brachypomus (370.21 ± 17.56 g). Fish were fed with pelleted practical diets to apparent satiation and the feces were collected by siphoning. The digestibility value for each ingredient was determined by comparison of the digestibility of a test diet with a reference diet (24.5% crude protein and 1% chromic oxide). The digestible energy values of soybean meal (SBM), fish meal (FM), corn (CN), and wheat bran (WB) were 2382, 3826, 3353, and 1784 kcal/kg, respectively. The apparent dry matter digestibility coefficients were 83.72, 90.14, 89.13, and 82.05% for SBM, FM, CN, and WB, respectively. The apparent crude protein digestibility coefficients were 75.88, 90.49, 85.06, and 61.62% for SBM, FM, CN, and WB, respectively. The apparent lipid digestibility coefficients were 63.03, 77.00, 83.01, and 82.45% for SBM, FM, CN, and WB, respectively. The digestibility of protein, lipid, and energy from SBM were somewhat low compared to values for other warmwater omnivorous fishes, but similar to values reported for pacu-caranha P. mesopotamicus. Otherwise, the nutrient and energy availability of the ingredients to P. brachypomus was similar to that of other fish. This information will be useful in formulating nutritious, economical diets for pacus. © by the World Aquaculture Society 2004.

Apparent digestibility of different ingredients in diets for bullfrog Rana catesbeiana tadpoles

The objective of this study was to determine apparent digestibility of protein and energy of ingredients commonly present in rations fed to bullfrog tadpoles Rana catesbeiana such as corn, soybean meal, wheat meal, rice bran, poultry by-product meal, blood meal, fish meal, corn starch and soybean oil. A completely randomized design was used to test ten experimental diets with four replicates. About 30% of the reference diet was replaced by the test ingredients, except for soybean oil that was used at 10% ratio and 0.5% of chromium oxide as inert marker. The values of apparent digestibility coefficient of crude protein (ADCCP) and gross energy (ADCGE) of the protein ingredients were, respectively: fish meal, 81 and 95%; poultry byproduct meal, 82 and 87%; blood meal, 64 and 53%; and soybean meal, 93 and 90%. While for the energetic ingredients, ADCCP and ADCGE, were, respectively: wheat meal, 100 and 72%; rice bran, 57 and 24%; and corn, 84 and 92%. Soybean oil and cornstarch ADCGE values were 70 and 96%, respectively; cornstarch digestibility is highly dependent on feed processing technique. The results showed that the ing redients of plant origin, except for rice bran, had good digestibility, thus indicating that tadpoles can effectively use plant protein. In this trial, the least digestible ingredients were blood meal and rice bran, thus indicating that the use of such ingredients in bullfrog tadpole diets should be limited. © Copyright by the World Aquaculture Society 2005.

Protein levels for heat-exposed broilers: Performance, nutrients digestibility, and energy and protein metabolism

Heat stress causes significant economic losses on broilers production due to poorer performance and carcass quality. Considering that protein has the highest heat increment among nutrients, it has been suggested that protein levels should be reduced in diets for heat-exposed broilers. Nevertheless, there are no conclusive results on the benefits of such practice, and further studies should be performed to elucidate some reported discrepancies. Thus, a trial was carried out to evaluate the effects of dietary protein levels (17, 20 and 23%) and environmental temperature (22 and 32°C) on the performance, nutrients digestibility, and energy and protein metabolism of broiler chickens from 21 to 42 days of age. Nutrients digestibility was determined by total excreta collection, and energy and protein metabolism was evaluated by comparative slaughter method. It was concluded that (1) heat exposure impairs broilers performance and increases nitrogen excretion, but do not change nutrients digestibility; (2) high-protein diets are technically feasible and promotes lower heat production for broilers reared under thermoneutral or hot environments, however, high-protein diets increases nitrogen excretion. © Asian Network for Scientific Information, 2007.

Consumo de capim-elefante com diferentes idades fornecido para vacas leiteiras

The 30, 45 and 60 day-old elephant grasses were evaluated by monitoring their nutritional profile and DM (DMI) and NDF (NDFI) intake by crossbred lactating cows in a 3 × 3 Latin square trial, where the split-plots were the rumen evacuation times: 0, 2, 4 and 6 h after meal. Chemical composition, in vitro dry matter digestibility and ruminal content ranged with grass age. Means (in kg/cow/day) of DMI and NDFI of 30 day-old grass (8.0 and 5.3) were lower than those of grasses with 45 days (10.0 and 6.6) and 60 days (11.0 and 7.3). The maximum of rumen fill was observed at 4:22, 3:55 and 3:49 hours after feeding for treatments with 30, 45 and 60 day-old elephant grass, respectively. Rumen fill did not limit the DMI and NDFI of elephant grass, but the dry matter intake of 30 day-old grass may be affected by the wet content.

Degradação ruminal e síntese de proteína microbiana em bovines alimentados com silagem de sorgo contendo tanino suplementado com concentrado ou uréia

This work aimed to determine ruminal parameters of dry mater (DM), neutral detergent fiber (NDF) and protein synthesis in the rumen of animals fed diets based on sorghum with different tannin levels. In situ degradation was evaluated by rumen incubation of level 1 (L1) and level 2 (L2) sorghum silages during 0, 6, 24, 48 and 96 hours. A duplicated 4 × 4 Latin Square was used. Potential degradability (PD) of DM was similar among silages, whereas the effective degradability (ED) decreased when the diets were supplemented with concentrate. Microbial nitrogen flux and microbial synthesis efficiency were not affected by roughage source. The synthesis efficiency, expressed in organic matter and crude protein digested in rumen, was higher in L1 tannin diets supplemented with concentrate. There was not relationship between the presence of tannins and the parameters of ruminal degradation.