Avaliação física, epidemiológica e molecular da infecção por Cryptosporidium spp. em passeriformes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ocorrência e caracterização molecular de Cryptosporidium spp. em aves selvagens brasileiras

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Characterization of 23S rRNA domain V mutations in gastric biopsy patients from the eastern Amazon

Resistance of Helicobacter pylori to clarithromycin is characterised by simple point mutations in the 23S ribosomal RNA (rRNA) gene and is responsible for the majority of cases of failure to eradicate this bacterium. In this paper, we characterised the variability of the 23S rRNA gene in biopsies of patients with gastric pathologies in the eastern Amazon (Northern Region of Brazil) using PCR and sequencing. A total of 49 sequences of H. pylori strains were analysed and of those, 75.6% presented nucleotide substitutions: A2142G (3.3%), T2182C (12.9%), G2224A (6.45%), T2215C (61.3%), A2192G (3.3%), G2204C (6.4%) and T2221C (6.4%). Of the mutations identified, four are known mutations related to cases of resistance and 16.1% are not yet described, revealing a high prevalence of mutations in the H. pylori 23S rRNA gene among the strains circulating in the in the eastern Amazon. The high prevalence in individuals with gastric pathologies in the Northern Region of Brazil demonstrates the need for characterising the profile of these strains to provide correct therapy for patients, considering that mutations in this gene are normally associated with resistance to the primary medication used in controlling H. pylori infection.

A comparison of molecular markers to detect Lutzomyia longipalpis naturally infected with Leishmania (Leishmania) infantum

The aim of the present study was to detect natural infection by Leishmania (Leishmania) infantum in Lutzomyia longipalpis captured in Barcarena, state of Pará, Brazil, through the use of three primer sets. With this approach, it is unnecessary to previously dissect the sandfly specimens. DNA of 280 Lu. longipalpis female specimens were extracted from the whole insects. PCR primers for kinetoplast minicircle DNA (kDNA), the mini-exon gene and the small subunit ribosomal RNA (SSU-rRNA) gene of Leishmania were used, generating fragments of 400 bp, 780 bp and 603 bp, respectively. Infection by the parasite was found with the kDNA primer in 8.6% of the cases, with the mini-exon gene primer in 7.1% of the cases and with the SSU-rRNA gene primer in 5.3% of the cases. These data show the importance of polymerase chain reaction as a tool for investigating the molecular epidemiology of visceral leishmaniasis by estimating the risk of disease transmission in endemic areas, with the kDNA primer representing the most reliable marker for the parasite.

The evolution of behavioural systems: a study of grooming in rodents

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Ocorrência da infecção por Cryptosporidium spp. em cabritos (Capra hircus)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The detection of Cryptosporidium serpentis in snake fecal samples by real-time PCR

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparative Chromosome Mapping of U2 snRNA and 5S rRNA Genes in Gymnotus Species (Gymnotiformes, Gymnotidae): Evolutionary Dynamics and Sex Chromosome Linkage in G. pantanal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Modelagem matemática de tumores e estequiometria biológica

Cancer biology is a complex and expanding field of science study. Due its complexity, there is a strong motivation to integrate many fields of knowledge to study cancer biology, and biological stoichiometry can make this. Biological stoichiometry is the study of the balance of multiple chemical elements in biological systems. A key idea in biological stoichiometry is the growth rate hypothesis, which states that variation in the carbon:nitrogen:phosphorus stoichiometry of living things is associated with growth rate because of the elevated demands for phosphorusrich ribosomal RNA and other elements necessary to protein synthesis. As tumor cells has high rate proliferation, the growth rate hypothesis can be used in cancer study. In this work the dynamic of two tumors (primary and secondary) and the chemical elements carbon and nitrogen are simulate and analyzed through mathematical models that utilize as central idea biological stoichiometry. Differential equations from mathematical model are solved by numerical method Runge-Kutta fourth order

Ocorrência e caracterização molecular de Cryptosporidium spp. em caprinos jovens do município de Quixadá-Ceará

Pós-graduação em Medicina Veterinária - FCAV

ITScan: a web-based analysis tool for Internal Transcribed Spacer (ITS) sequences

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

First detection of Leishmania spp. DNA in Brazilian bats captured strictly in urban areas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

First genetic identification of Cryptosporidium parvum subtype IIaA14G2R1in beef cattle in Brazil

The presence of Cryptosporidium spp. in a cattle herd registered with an outbreak of diarrhea was investigated and the the molecular subtyping of Cryptosporidium parvum was characterized. Fecal samples from 85 Nellore beef cattle (Bos indicus) were collected and examined with Ziehl-Neelsen modified staining method. Fifty-four cattle (63.52%) had Cryptosporidium spp. oocysts in their feces. Fragments of genes encoding the 18S ribosomal RNA subunit and a 60-kDa glycoprotein (gp60) were amplified by nested PCR accomplished in the 11 most heavily parasitized samples, and the amplicons were sequenced. Eight of the 11 analyzed samples were positive for 18S rRNA sequences and identified monospecific infections with C. parvum. Seven samples were positive for gp60 and identified subtypes IIaA15G2R1 (6/11) and IIaA14G2R1 (1/11). This report is the first for C. parvum subtype IIaA14G2R1 in beef cattle in Brazil.

Francisella noatunensis orientalis em tilápias (Oreochromis niloticus) cultivadas em tanques-rede na bacia hidrográfica do rio Araguari-Minas Gerais

Pós-graduação em Medicina Veterinária - FMVZ 33004064022P3

Francisella noatunensis orientalis em tilápias (Oreochromis niloticus) cultivadas em tanques-rede na bacia hidrográfica do rio Araguari-Minas Gerais

Pós-graduação em Medicina Veterinária - FMVZ