Identificação de Enterobacter spp. através de técnicas convencionais e do sistema semi-automatizado Microscan (Autoscan-4)

A hundred and ninety-five (195) strains of Enterobacter spp., isolated from diverse clinical specimens - urine, feces, cateter, blood, wound, tracheal aspirate, vaginal fluid - were submitted to the conventional identification by biochemical tests, and were also submitted to the identification by panels NegCombo 20 of the system automated MicroScan - AutoScan- 4 (Dade Behring Inc., West Sacramento, CA, USA). The samples were from patients of the Clinical Laboratory from the School of Pharmacy and Biochemistry of UNOESTE, Presidente Prudente, SP, and from patients hospitalized at the University Hospital Domingos Leonardo Cerávolo, UNOESTE. Of the total of strains tested, 191 (97.9%) presented agreement between the two approaches utilized and 4 strains (2.1%) presented identification disagreement, that is, the genus identified was different in each approach. By this study, the conclusion is that both the approaches utilized for the identification presented advantages and disadvantages related to the cost, facility of execution, quickness, reliability and some other characteristics. Even so, our results showed that conventional methods represent a reliable tool for Enterobacter identification.

Multiplex PCR use for Staphylococcus aureus identification and oxacillin and mupirocin resistance evaluation

Oxacillin-resistant Staphylococcus aureus represents a serious problem in hospitals worldwide, increasing infected patients' mortality and morbidity and raising treatment costs and internment time. In this study, the results of using the Multiplex PCR technique to amplify fragments of the genes femA (specific-species), mecA (oxacillin resistance) and ileS-2 (mupirocin resistance) were compared with those of tests conventionally used to identify S. aureus isolates and ascertain their resistance to drugs. Fifty S. aureus strains were isolated from patients receiving treatment at UNOESTE University Hospital in Presidente Prudente, SP, Brazil. The 686 bp fragment corresponding to the gene femA was amplified and detected in all the isolates. On the other hand, the 310 bp fragment corresponding to the mecA gene was amplified in 29 (58%) of the isolates. All of the isolates showed sensitivity to mupirocin in the agar diffusion test, which was corroborated by the lack of any amplicon of the 456 bp fragment corresponding to the ileS-2 gene, in the PCR bands. The conventional tests to identify S. aureus and detect resistance to oxacillin and mupirocin showed 100% agreement with the PCR Multiplex results. The use of techniques for rapid and accurate identification of bacteria and assessment of their resistance may be valuable in the control of infection by resistant strains, allowing the rapid isolation and treatment of an infected patient. However, the results demonstrate that traditional phenotypic tests are also reliable, though they take more time.

Proposição de rotina morfológica para detecção de malha viária em imagens orbitais

The outdating of cartographic products affects planning. It is important to propose methods to help detect changes in surface. Thus, the combined use of remote sensing image and techniques of digital image processing has contributed significantly to minimize such outdating. Mathematical morphology is an image processing technique which describes quantitatively geometric structures presented in the image and provides tools such as edge detectors and morphological filters. Previous studies have shown that the technique has potential on the detection of significant features. Thus, this paper proposes a routine of morphological operators to detect a road network. The test area corresponds to an excerpt Quickbird image and has as a feature of interest an avenue of the city of Presidente Prudente, SP. In the processing, the main morphological operators used were threshad, areaopen, binary and erosion. To estimate the accuracy with which the linear features were detected, it was done the analysis of linear correlation between vectors of the features detected and the corresponding topographical map of the region. The results showed that the mathematical morphology can be used in cartography, aiming to use them in conventional cartographic updating processes.

Associação entre composição corporal, equilíbrio e mobilidade de idosos com 80 anos ou mais

Study design: cross-sectional study. Objective: The objective of this study was to analyze the association between body composition, mobility and balance of elderly aged 80 years or older. Methods: The sample consisted of 123 elderly aged 80 and 95 years (83.2 ± 2.7 years) with 78 women (83.2 ± 2.9 years) and 45 men (83.2 ± 2, 4 years) residing in the city of Presidente Prudente - SP. Assessment of body composition was made by absorpiometria dual energy X-ray (DXA). The mobility and balance were assessed by means of tests of speed walking, and static balance lower limb strength. For statistical analysis we carried out the chi-square test, the software used was SPSS (13.0) and the significance level was set at 5%. Results: In males, those with higher performance in the functional tests showed higher percentages of muscle mass (MM) (35.6%) compared to lower performance (15.6%), p = 0.026. In the female group, the elderly with higher performance on tests showed higher bone mineral density (BMD) values (30.8%) compared with those with lower performance (20.5%) p = 0.041. Conclusion: MM for elderly males and BMD for females were the components of body composition that is associated with functional capacity.

Associação entre fatores de risco cardiovascular e capacidade funcional de idosos longevos

Study design: cross-sectional study. Objective: To assess the association between the presence of cardiovascular risk factor (CRP) and functional capacity of the oldest old. Methods: The sample 9onsisted of 91 elderly aged 80 and 90 years (83.0 ± 2.5 years) with 60 women (82.2 ± 2.1 years) and 31 men (83.2 ± 2,6 years) residing in the city of Presidente Prudente - SP. The FRC were analyzed: arterial hypertension (AH) and excess body fat (total and trunk). The presence of hypertension was verified by means of self-reported questionnaire based on the Standard Health Questionnaire (SHQ). Assessment of body was made by absorpiometria dual energy X-ray absorptiometry (DXA) and functional capacity was assessed by the functional tests (static balance, normal walking speed and force of the lower limbs). For statistical analysis we carried out the chi-square test, the software used was SPSS (13.0) and the significance level was set at 5%. Results. In males, with hypertension and the presence of excess %BF had lower performance in the lower limbs (83.3% lower and 16.7% higher), p = 0.011 compared to those with only a VCF. The elderly women with hypertension and the presence of excess GTron% also had lower performance on the same test (80.6% lower and 19.4% higher), p = 0.018 and the test of walking speed (80.6% lower and 19.4% higher), p = 0.034. Conclusion: Arterial hypertension and excess body fat (total and trunk) aggregated are FRC, which are associated with reduced functional capacity of the oldest old.

Seasonal effects on semen and testosterone in zebu and taurine bulls

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Que tipo de estado? que tipo de igualdade? XI Conferencia Regional sobre a Mulher da América Latina e do Caribe: Brasília, 13 a 16 de julho de 2010. Síntese

No documento Que tipo de Estado? Que tipo de igualdade? analisa-se o progresso da igualdade de gênero na Região depois de 15 anos da aprovação da Plataforma de Ação de Beijing, 10 anos da formulação dos Objetivos de Desenvolvimento do Milênio e 3 anos da adoção do Consenso de Quito, na décima Conferência Regional sobre a Mulher da América Latina e do Caribe realizada em 2007. Examinam-se, além disso, as conquistas e desafios que enfrentam os governos à luz da interação entre o Estado, o mercado e as famílias como instituições sociais construídas a partir de políticas, leis, usos e costumes que, em conjunto, estabelecem as condições para renovar ou perpetuar as hierarquias sociais e de gênero.

Que tipo de Estado? que tipo de igualdade?: Conferência Regional sobre a Mulher da América Latina e do Caribe: Brasilia, 13 al 16 de julio de 2010

No documento Que tipo de Estado? Que tipo de igualdade? analisa-se o progresso da igualdade de gênero na Região depois de 15 anos da aprovação da Plataforma de Ação de Beijing, 10 anos da formulação dos Objetivos de Desenvolvimento do Milênio e 3 anos da adoção do Consenso de Quito, na décima Conferência Regional sobre a Mulher da América Latina e do Caribe realizada em 2007. Examinam-se, além disso, as conquistas e desafios que enfrentam os governos à luz da interação entre o Estado, o mercado e as famílias como instituições sociais construídas a partir de políticas, leis, usos e costumes que, em conjunto, estabelecem as condições para renovar ou perpetuar as hierarquias sociais e de gênero.

Análise da incerteza na representação de classes temáticas resultantes da aplicação de uma rede neural artificial

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Detecção de rodovias em imagens digitais de alta resolução com o uso da teoria de morfologia matemática

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Acurácia na determinação das coordenadas dos vértices de imóveis urbanos

Pós-graduação em Ciências Cartográficas - FCT

Integração de imagens vetoriais e matriciais na visualização dos levantamentos realizados com a unidade móvel de mapeamento digital

Pós-graduação em Ciências Cartográficas - FCT

Detecção de áreas de oclusão para geração de ortoimagem verdadeira utilizando dados LASER

Pós-graduação em Ciências Cartográficas - FCT

O vestuário do negro na fotografia e na pintura: Brasil, 1850-1890

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise de fatores biológicos de indivíduos com 80 anos ou mais e que apresentam relação com suas longevidades

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)