Regulation of self-renewal and detection of karyotypic changes of pluripotent human embryonic stem cells

Human embryonic stem cells are pluripotent cells capable of renewing themselves and differentiating to specialized cell types. Because of their unique regenerative potential, pluripotent cells offer new opportunities for disease modeling, development of regenerative therapies, and treating diseases. Before pluripotent cells can be used in any therapeutic applications, there are numerous challenges to overcome. For instance, the key regulators of pluripotency need to be clarified. In addition, long term culture of pluripotent cells is associated with the accumulation of karyotypic abnormalities, which is a concern regarding the safe use of the cells for therapeutic purposes. The goal of the work presented in this thesis was to identify new factors involved in the maintenance of pluripotency, and to further characterize molecular mechanisms of selected candidate genes. Furthermore, we aimed to set up a new method for analyzing genomic integrity of pluripotent cells. The experimental design applied in this study involved a wide range of molecular biology, genome-wide, and computational techniques to study the pluripotency of stem cells and the functions of the target genes. In collaboration with instrument and reagent company Perkin Elmer, KaryoliteTM BoBsTM was implemented for detecting karyotypic changes of pluripotent cells. Novel genes were identified that are highly and specifically expressed in hES cells. Of these genes, L1TD1 and POLR3G were chosen for further investigation. The results revealed that both of these factors are vital for the maintenance of pluripotency and self-renewal of the hESCs. KaryoliteTM BoBsTM was validated as a novel method to detect karyotypic abnormalities in pluripotent stem cells. The results presented in this thesis offer significant new information on the regulatory networks associated with pluripotency. The results will facilitate in understanding developmental and cancer biology, as well as creating stem cell based applications. KaryoliteTM BoBsTM provides rapid, high-throughput, and cost-efficient tool for screening of human pluripotent cell cultures.

Conditions and practices in the commercialisation of innovations in wood industry

The report 'Conditions and practices in the commercialisation of innovation in wood industry' has been written as a part of the Wood Academy project. The report analyses the commercialisation conditions and practices of wood industry by utilising product categorisation based on a conceptual schema which combines the aspects of the transfer of the procession of utility and the degree of form/service utility (or value-added) created or provided by the company. Open innovation approaches help to perceive the possible new product and service innovations as well as the new business models and earning logics in the industry. The report also contains brief company cases to demonstrate theory-to-practice and showcase company examples from successful Finnish companies.

Geochemistry and water quality of Lake Qarun, Egypt

Water geochemistry is a very important tool for studying the water quality in a given area. Geology and climate are the major natural factors controlling the chemistry of most natural waters. Anthropogenic impacts are the secondary sources of contamination in natural waters. This study presents the first integrative approach to the geochemistry and water quality of surface waters and Lake Qarun in the Fayoum catchment, Egypt. Moreover, geochemical modeling of Lake Qarun was firstly presented. The Nile River is the main source of water to the Fayoum watershed. To investigate the quality and geochemistry of this water, water samples from irrigation canals, drains and Lake Qarun were collected during the period 2010‒2013 from the whole Fayoum drainage basin to address the major processes and factors governing the evolution of water chemistry in the investigation area. About 34 physicochemical quality parameters, including major ions, oxygen isotopes, trace elements, nutrients and microbiological parameters were investigated in the water samples. Multivariable statistical analysis was used to interpret the interrelationship between the different studied parameters. Geochemical modeling of Lake Qarun was carried out using Hardie and Eugster’s evolutionary model and a model simulated by PHREEQC software. The crystallization sequence during evaporation of Lake Qarun brine was also studied using a Jänecke phase diagram involving the system Na‒K‒Mg‒ Cl‒SO4‒H2O. The results show that the chemistry of surface water in the Fayoum catchment evolves from Ca- Mg-HCO3 at the head waters to Ca‒Mg‒Cl‒SO4 and eventually to Na‒Cl downstream and at Lake Qarun. The main processes behind the high levels of Na, SO4 and Cl in downstream waters and in Lake Qarun are dissolution of evaporites from Fayoum soils followed by evapoconcentration. This was confirmed by binary plots between the different ions, Piper plot, Gibb’s plot and δ18O results. The modeled data proved that Lake Qarun brine evolves from drainage waters via an evaporation‒crystallization process. Through the precipitation of calcite and gypsum, the solution should reach the final composition "Na–Mg–SO4–Cl". As simulated by PHREEQC, further evaporation of lake brine can drive halite to precipitate in the final stages of evaporation. Significantly, the crystallization sequence during evaporation of the lake brine at the concentration ponds of the Egyptian Salts and Minerals Company (EMISAL) reflected the findings from both Hardie and Eugster’s evolutionary model and the PHREEQC simulated model. After crystallization of halite at the EMISAL ponds, the crystallization sequence during evaporation of the residual brine (bittern) was investigated using a Jänecke phase diagram at 35 °C. This diagram was more useful than PHREEQC for predicting the evaporation path especially in the case of this highly concentrated brine (bittern). The predicted crystallization path using a Jänecke phase diagram at 35 °C showed that halite, hexahydrite, kainite and kieserite should appear during bittern evaporation. Yet the actual crystallized mineral salts were only halite and hexahydrite. The absence of kainite was due to its metastability while the absence of kieserite was due to opposed relative humidity. The presence of a specific MgSO4.nH2O phase in ancient evaporite deposits can be used as a paleoclimatic indicator. Evaluation of surface water quality for agricultural purposes shows that some irrigation waters and all drainage waters have high salinities and therefore cannot be used for irrigation. Waters from irrigation canals used as a drinking water supply show higher concentrations of Al and suffer from high levels of total coliform (TC), fecal coliform (FC) and fecal streptococcus (FS). These waters cannot be used for drinking or agricultural purposes without treatment, because of their high health risk. Therefore it is crucial that environmental protection agencies and the media increase public awareness of this issue, especially in rural areas.

Logistics Cost Optimization of Road Freight Shipments in a Consumer Goods Company

In the industry of the case company, transportation and warehousing costs account for more than 10% of the total cost which is more than on average. A Finnish company has an understanding that by sending larger shipments in parcels, they could save tens of thousands of euros annually in freight costs in Finland’s domestic shipments. To achieve these savings and optimize total logistics cost, company���s interest is to find out which is the cost efficient way of shipping road shipments of certain volumes; in parcel boxes or on pallets, and what should be the split volume determining the shipment type. Distribution center (DC) costs affect this decision and therefore they need to be also evaluated to determine the total logistics cost savings. Main results were achieved by executing activity-based costing-calculations including DC and road freight costs to determine the ideal split volume with which the total logistics cost is optimal. Calculations were done for Finland’s DC, separately for two main road freight destinations, Finland and Sweden, which cover 50% of road shipment spend. Data for calculations was collected both manually and automatically from various internal and external sources, such as the company ERP system and logistics service providers’ (LSP) reporting. DC processes were studied in practice and compared to model processes. Currently used freight rates were compared to existing pricing models and freight service tendering process was evaluated by participating in the process and comparing it to the models based on literature. The results show that the potential savings are not as significant as the company hoped for, mainly because of packing work increasing DC labor cost. Annual savings by setting ideal split volume per country would account for 0,4 % of the warehousing and transportation costs of shipments in scope of this thesis. Split volume should be set separately for each route, mainly because the pricing model for road freight is different in each country. For some routes bigger parcels should be sent but for some routes pallets should be used more. Next step is to do these calculations for remaining routes to determine total savings potential. Other findings show that the processes in the DC are designed well and the company could achieve savings by executing tenders more efficiently. Company should also pay more attention to parcel pricing and packing the shipments accordingly.

THE ADAPTATION PROCESS AND CHALLENGES OF EDUCATION EXPORT

The purpose of this study is to investigate the challenges of the adaptation process of education export. The research is conducted as a single case study that concentrates on three education export projects. The case company in the research is Team Academy. The study goes through the different forms of education export, the adaptation of education export and the challenges of the education export –process by means of theory and empirical data. The research is carried out as a qualitative research and the method used is a qualitative content analysis. More specifically the research is an abductive content analysis. The research data is collected in four in-depth interviews from Team academy representatives who have been strongly involved in certain education export –project of Team Academy. The research confirms the theory in the challenge of hierarchy, funding and registration issues, and refutes it in the challenge of competition, legislation, different governmental attitudes and knowledge in productization. The main challenges of the adaptation process are related to funding, differences in values, sudden changes, the complex nature of the learning model, concept of time, teamwork as method and accreditation. It is highlighted that in the future operations, anticipating problems that arise from for example cultural differences and differences in values, communication, managing the money flows and the company form is recommended. Future research could continue with investigating the suitable company form for education exports of this kind, and how to stand out and communicate when operating under another institution. It is considered a potential risk that a brand encloses the brand that operates under it.

Development of a New Welding Product Quality Control and Management System Model for China

The Chinese welding industry is growing every year due to rapid development of the Chinese economy. Increasingly, companies around the world are looking to use Chinese enterprises as their cooperation partners. However, the Chinese welding industry also has its weaknesses, such as relatively low quality and weak management. A modern, advanced welding management system appropriate for local socio-economic conditions is required to enable Chinese enterprises to enhance further their business development. The thesis researches the design and implementation of a new welding quality management system for China. This new system is called ‗welding production quality control management model in China‘ (WQMC). Constructed on the basis of analysis of a survey and in-company interviews, the welding management system comprises the following different elements and perspectives: a ‗Localized congenital existing problem resolution strategies‘ (LCEPRS) database, a ‗human factor designed training system‘ (HFDT) training strategy, the theory of modular design, ISO 3834 requirements, total welding management (TWM), and lean manufacturing (LEAN) theory. The methods used in the research are literature review, questionnaires, interviews, and the author‘s model design experiences and observations, i.e. the approach is primarily qualitative and phenomenological. The thesis describes the design and implementation of a HFDT strategy in Chinese welding companies. Such training is an effective way to increase employees‘ awareness of quality and issues associated with quality assurance. The study identified widely existing problems in the Chinese welding industry and constructed a LCEPRS database that can be used in efforts to mitigate and avoid common problems. The work uses the theory of modular design, TWM and LEAN as tools for the implementation of the WQMC system.

Water-power at Niagara Falls to be successfully utilized : its objects, facilities and resources, inducements for manufacturers, etc. /

The lithograph, "General view of lands, tunnel and docks of Niagara River Hydraulic Tunnel, Power and Sewer Company," called for p. [4] in the Index, has been removed and encapsulated, and is shelved separately.

St. Catharines documents including mortgages, wills and declarations, 1871-1914 (not inclusive)

Most of these documents refer to a tract of land located on the intersection of King and Court Streets (part of the Lawrie plan) and Carter properties in St. Catharines Ontario. The Security Loan and Savings Company of St. Catharines existed between 1870 and 1906. Thomas Rodman Merritt was the president.

Indenture of Release to Woodstock and Lake Erie Railway from Executors of Samuel Zimmerman

Indenture of release regarding a loan of 12, 500 pounds which Samuel Zimmerman (deceased) is said to have lent and advanced to the Woodstock and Lake Erie Railway and Harbour Company. This is put forward by the executors who include: Joseph A. Woodruff, Richard Woodruff, John L. Ranney and Richard Miller. This document releases and exonerates the Railway Company from any debts to the executors. [The outside of the document says 1857, but the actual date is Feb. 10, 1858].

Receipt from N. W. Harris and Co., Bankers, New York

Receipt from N. W. Harris and Co., Bankers, New York for installment on Dominion Power and Transmission Co., Dec. 19, 1907.

Blenkhorn and Sawle Fonds, 1946-1961, n.d. (non-inclusive)

Correspondence, flyers and clippings regarding the Blenkhorn and Sawle Company.

Le statut juridique du courriel au Canada et aux États-Unis

Cet article aborde le degré de protection en matière de vie privée accordé au courrier électronique par les tribunaux nord-américains. L’auteur dresse un portrait général de la jurisprudence canadienne et américaine actuellement disponible en la matière, en concentrant ses propos sur l’expectative de vie privée des employés ainsi que sur les droits de l’employeur d’accéder à leurs courriels dans un contexte de relations de travail. Alors que les tribunaux canadiens abordent la problématique en procédant par analogie, c’est-à-dire par comparaison du courriel avec des modes de communication plus traditionnels (i.e. le téléphone et le courrier), les tribunaux américains font une analyse interprétative du 4ème amendement de la constitution des États-Unis et des droits statutaires accordés par l’Electronic Communications Privacy Act de 1986 et le Privacy Protection Act de 1986. L’auteur précise que les dispositions législatives actuelles présentent d’importantes lacunes rendant difficile leur application aux courriels.

Oportunidades y amenazas para Colombia de pertenecer a la Alianza del Pacífico

Por medio de este proyecto se analizaron las asimetrías presentes en las economías de los cuatro países miembros de la Alianza del Pacífico y cómo Colombia puede enfrentar estos y retos y convertirlos en oportunidades de acuerdo a la experiencia en diferentes áreas de sus países aliados. De acuerdo a las bases de datos de los países firmantes se encontró que la balanza comercial entre Colombia y México presenta un déficit y está en constante crecimiento, pudiendo ser contrarrestada con futuras negociaciones y especialización de la industria agropecuaria tomando mejores medidas sanitarias y fitosanitarias requeridas por dichos mercados. Igualmente se abren las puertas para ingresar a acuerdos con gran índole mundial como el TPP, al cual la República de Chile, los Estados Unidos Mexicanos y la República del Perú ya hacen parte lo cual representa para Colombia una oportunidad para penetrar al mercado de Asia – Pacífico y buscar la perdurabilidad de las empresas, industrias y sectores dentro de un contexto global.

Serie de documentos. Facultad de Jurisprudencia N° 72. Aspectos prácticos del proceso ante el Centro Internacional de Arreglo de Diferencias relativas a Inversiones (CIADI)

Los TBI (Tratado Bilateral de Inversión) o también denominado los APPI (Acuerdo de Protección y Promoción de las Inversiones) son acuerdos que buscan la Protección y la Promoción de las inversiones que hagan los nacionales de un Estado en otro Estado conocido como el “Estado Receptor”. Los TBI y APPI fueron concebidos dentro del proceso de descolonización que le siguió a la Segunda Guerra Mundial. Esto se debe a que los Estados que habían colonizado otros territorios, ahora Estados soberanos y sus nacionales, seguían teniendo fuertes lazos e intereses económicos. Con el ánimo de protegerse de posibles expropiaciones, los Estados europeos liderados por Alemania, firmaron un número importante de TBI y APPI, no solamente con sus antiguas colonias, sino con otras. De esta manera, Alemania, Suiza, Holanda, Italia, Bélgica y Suecia suscribieron al menos cuarenta tratados de este tipo con varios Estados asiáticos, africanos y del medio oriente entre 1959 y 1965. Estados Unidos se demoró unos veinte o treinta años más, porque los estadounidenses no solamente aspiraban a obtener protección para sus inversionistas, sino que además solicitaban que se abriera y liberara la economía de los Estados firmantes. Finalmente, fue en la administración de Ronald Reagan que los Estados Unidos comenzó a suscribirlos.