904 resultados para Maria Isabel de Austria, 1680-1741


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The mapping of the land use, vegetation and environmental impacts using remote sensing and geoprocessing allows detection, spatial representation and quantifying all alterations due to the human action in the nature, contributing to the monitoring and planning of those activities that cause damages to the environment. The aim of this research is analyze the transformation ocurred with the land use and vegetation in order to detect environmental impacts during the period from 1962 to 1995, considering a test area in the district of Assistência and surroundings, in the Rio Claro (SP) region. In order to archieve such aim the authors used boolean operations available in the Geographical Information System (GIS) - Idrisi. The maps were obtained through the ordinary (conventional) interpretation of aerial photos, later digitized in the software CAD Overlay and georeferenced in AutoCAD Map. It's observed that operations such as crossing digitized maps of one specific area in two differents dates, using GIS, produce overall results that might point out expansion or retraction's trends of the mapped classes, as well as quantify the intensity of the phenomena.

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The mapping of the land use, vegetation and environmental impacts using remote sensing ana geoprocessmg allow detection, spatial representation and quantification of the alterations caused by the human action on the nature, contributing to the monitoring and planning of those activities that may cause damages to the environment. This study apply methodologies based on digital processing of orbital images for the mapping of the land use, vegetation and anthropic activities that cause impacts in the environment. It was considered a test area in the district of Assistência and surroundings, in Rio Claro (SP) region. The methodology proposed was checked through the crossing of maps in the software GIS - Idrisi. These maps either obtained with conventional interpretation of aerial photos of 1995, digitized in the software CAD Overlay and geo-referenced in the AutoCAD Map, or with the application of digital classification systems on SPOT-XS and PAN orbital images of 1995, followed by field observations. The crossing of conventional and digital maps of a same area with the CIS allows to verify the overall results obtained through the computational handling of orbital images. With the use of digital processing techniques, specially multiespectral classification, it is possible to detect automatically and visually the impacts related to the mineral extraction, as well as to survey the land use, vegetation and environmental impacts.

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The possibilities of using the sperm collected from the epididymis have been widely used because the fertilizing capacity sperm preservation and the possibility of using it for wild cats. But in the process of cryopreservation, some studies show a decrease in the quality of the sperm when left under cooling before frozen for some time. This study aimed to assess the quality of the epididymal sperm obtained from domestic cats after cryopreservation using a diluent based on egg yolk and glycerol (Botu-crio®), comparing the morphofunctional characteristics after cooling for 24 hours in a container of semen transport (Botu-tainer®). We use eight cats submitted to elective orchiectomy, aging from eight months, without racial determination, and good nutritional status. These sperm characteristics were: motility, vigor, concentration, membrane integrity and morphology. It has been found, after statistical analysis, that the container of semen was able to maintain sperm viability, even for 24h. We also observed a significant decrease on all parameters after frozen, consequential, probably to thermal stress that occurs in processing. However, the percentage of membrane integrity after thawing shows good employability of the Botu-crio®, which viability is possible to perform in vitro fertilization, requiring higher ratings.

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This research aims at performing a comparative study between the Brazilian scientific production in Dentistry, from 2000 to 2009 and countries that contribute with at least 2 % of the world's scientific production indexed in the Scopus database. More specifically, we intend to assess the annual Brazilian scientific production by comparing it to the other countries', analyze the Brazilian and other countries' publications in journals with higher impact factors, as well as to highlight the scientific production from these countries and its international visibility, measured by its total and by its average of citations and normalized citation index per year, by comparing the countries, and to compare the index h of such countries. As work procedure, the SCImago Journal and Country Rank was used as source, identifying the group of producing countries in the Dentistry area from 1996 to 2009. From a total of 136 countries, 13 were highlighted as the most productive, each one of them accounting for at least 2 % the worldwide scientific production in the area. The following indicators were raised for each country: number of produced documents, total of citations, self-citations, average of citations per document and index h. We verified that Brazil is the only country in Latin America that is pictured among the most productive ones in the Dentistry area. We observed that Brazil presents a growing visibility and impact in the international scenery, what suggests that its production is constantly consolidating, with Brazilian scientific recognition in the main vehicles of dissemination in the area. © 2012 Akadémiai Kiadó, Budapest, Hungary.

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This study evaluated the influence of intensive farming of tilapia on physical and chemical parameters and on the occurrence of Streptococcus spp. in the water of the lake and of cages. Throughout a year, monthly samplings were taken in the rainy and dry seasons for a year, at two sampling sites, lake and net cages. For the determination of water quality, physical and chemical water parameters were evaluated and compared to the standards established by Conama Resolution no. 357/2005. The presence of Streptococcus spp. in the water was determined by plating on blood Agar and biochemical screening. Mean values of water parameters were tested using the Kruskal-Wallis test comparing sampling sites and seasons. Ammoniacal nitrogen (ammoniacal-N), total phosphorus (total-P) levels and occurrence of Streptococcus spp. have increased in the water of the net cages. The mean values of several parameters have decreased during the rainy period, except for pH, temperature and ammoniacal-N. Total-P and dissolved oxygen levels, during dry and rainy periods, respectively, exceeded the standard established for freshwater class 2, recommended for aquaculture, which can be harmful to the fish. Therefore, constant monitoring of the physical,chemical and microbiological water parameters is recommended since the Juara lake is also used for recreational purposes.

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Purpose: The article aims to introduce nurses to how genetics-genomics is currently integrated into cancer care from prevention to treatment and influencing oncology nursing practice. Organizing Construct: An overview of genetics-genomics is described as it relates to cancer etiology, hereditary cancer syndromes, epigenetics factors, and management of care considerations. Methods: Peer-reviewed literature and expert professional guidelines were reviewed to address concepts of genetics-genomics in cancer care. Findings: Cancer is now known to be heterogeneous at the molecular level, with genetic and genomic factors underlying the etiology of all cancers. Understanding how these factors contribute to the development and treatment of both sporadic and hereditary cancers is important in cancer risk assessment, prevention, diagnosis, treatment, and long-term management and surveillance. Conclusions: Rapidly developing advances in genetics-genomics are changing all aspects of cancer care, with implications for nursing practice. Clinical Relevance: Nurses can educate cancer patients and their families about genetic-genomic advances and advocate for use of evidence-based genetic-genomic practice guidelines to reduce cancer risk and improve outcomes in cancer management. © 2013 Sigma Theta Tau International.

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We have previously shown that the subunit 1 of Leishmania amazonensis RPA (LaRPA-1) alone binds the G-rich telomeric strand and is structurally different from other RPA-1. It is analogous to telomere end-binding proteins described in model eukaryotes whose homologues were not identified in the protozoan's genome. Here we show that LaRPA-1 is involved with damage response and telomere protection although it lacks the RPA1N domain involved with the binding with multiple checkpoint proteins. We induced DNA double-strand breaks (DSBs) in Leishmania using phleomycin. Damage was confirmed by TUNEL-positive nuclei and triggered a G1/S cell cycle arrest that was accompanied by nuclear accumulation of LaRPA-1 and RAD51 in the S phase of hydroxyurea-synchronized parasites. DSBs also increased the levels of RAD51 in non-synchronized parasites and of LaRPA-1 and RAD51 in the S phase of synchronized cells. More LaRPA-1 appeared immunoprecipitating telomeres in vivo and associated in a complex containing RAD51, although this interaction needs more investigation. RAD51 apparently co-localized with few telomeric clusters but it did not immunoprecipitate telomeric DNA. These findings suggest that LaRPA-1 and RAD51 work together in response to DNA DSBs and at telomeres, upon damage, LaRPA-1 works probably to prevent loss of single-stranded DNA and to assume a capping function.

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Trypanosoma cruzi comprises a pool of populations which are genetically diverse in terms of DNA content, growth and infectivity. Inter- and intra-strain karyotype heterogeneities have been reported, suggesting that chromosomal rearrangements occurred during the evolution of this parasite. Clone D11 is a single-cell-derived clone of the T. cruzi G strain selected by the minimal dilution method and by infecting Vero cells with metacyclic trypomastigotes. Here we report that the karyotype of clone D11 differs from that of the G strain in both number and size of chromosomal bands. Large chromosomal rearrangement was observed in the chromosomes carrying the tubulin loci. However, most of the chromosome length polymorphisms were of small amplitude, and the absence of one band in clone D11 in relation to its reference position in the G strain could be correlated to the presence of a novel band migrating above or below this position. Despite the presence of chromosomal polymorphism, large syntenic groups were conserved between the isolates. The appearance of new chromosomal bands in clone D11 could be explained by chromosome fusion followed by a chromosome break or interchromosomal exchange of large DNA segments. Our results also suggest that telomeric regions are involved in this process. The variant represented by clone D11 could have been induced by the stress of the cloning procedure or could, as has been suggested for Leishmania infantum, have emerged from a multiclonal, mosaic parasite population submitted to frequent DNA amplification/deletion events, leading to a 'mosaic' structure with different individuals having differently sized versions of the same chromosomes. If this is the case, the variant represented by clone D11 would be better adapted to survive the stress induced by cloning, which includes intracellular development in the mammalian cell. Karyotype polymorphism could be part of the T. cruzi arsenal for responding to environmental pressure. © 2013 Lima et al.

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The physiological control to support the absence of O2 for long periods of diving, and oxidative damage impact caused by the whole process of hypoxia/reperfusion in freshwater turtles is well known. However, effects of contaminants may act as co-varying stressors and cause biological damage, disrupting the hypoxia/reperfusion oxidative damage control. In order to investigate the action of environmental stressors present in domestic or industrial wastewater effluent, we performed a biochemical analysis of biotransformation enzymes, oxidative stress, as well as neuromuscular, physiological and morphological parameters in Phrynops geoffroanus, an hypoxic-tolerant freshwater turtle endemic of South America, using animals sampled in urban area, contaminated by sewage and industrial effluents and animals sampled in control area. Here we demonstrate the physiological and biochemical impact caused by pollution, and the effect that these changes cause in antioxidant activity. Animals from the urban area exhibited higher EROD (ethoxyresorufin-O-deethylase, CYP1A1), GST (glutathione S-transferase), G6PDH (glucose-6-phosphate deshydrogenase), AChE (acetilcholinesterase) activities and also TEAC (trolox-equivalent antioxidant capacity) and TBARS (thiobarbituric acid reactive substances) values. We examined whether two morphometric indices (K - condition factor and HIS - hepatosomatic index) which help in assessing the general condition and possible liver disease, respectively, were modified. The K of the urban animals was significantly decreased compared to the control animals, but the HIS value was increased in animals from the urban area, supporting the idea of an impact in physiology and life quality in the urban freshwater turtles. We propose that this freshwater turtle specie have the ability to enhance its antioxidants defenses in order to protect from tissue damage caused by hypoxia and reperfusion, but also that caused by environmental contamination and that the oxidative damage control in hypoxic conditions has resulted in an adaptive condition in hypoxic-tolerant freshwater turtle species, in order to better tolerate the release of contaminated effluents resulting from human activity. © 2013 Elsevier Inc.

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Pós-graduação em Educação Escolar - FCLAR

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Pós-graduação em Educação Matemática - IGCE

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)