Comportamento de híbridos de milho transgênicos e respectivos híbridos isogênicos convencionais em relação à infestação de Spodoptera Frugiperda (j. E. Smith,1727) (Lepidoptera: Noctuidae) em duas localidades e épocas de semeadura

The fall armyworm Spodoptera frugiperda is one of the most important pests of maize. Various studies are conducted for their management, integrating chemical and biological control tactics as well as resistant plants. In order to offer alternatives for an efficient management of this pest with minimal use of pesticides, the technology of genetically modified plants resistant to insects has been widely studied. The aim of the present study was to evaluate the natural infestation of larvae of S. frugiperda and their injuries under field conditions in transgenic maize hybrids compared to their conventional isogenic counterparts at two sowing dates and two regions. The hybrids were planted in the off season of 2010 in Jaboticabal, SP, Brazil, and the summer of 2010/2011 in Jaboticabal, and Pindorama, SP, in a randomized block with seven treatments (hybrids) and four replications. Different levels of infestation of larvae occurred throughout the phenological development of plants in conventional and genetically modified hybrids with significant differences between the two groups in most evaluations. The hybrid 2B710HX was the least infested with caterpillars and had the least damaged leaf area. It follows that the Cry1F toxin was the most effective in protecting the plant in relation to other toxic proteins expressed by the other Bt hybrids against infestation and damage promoted by this pest, regardless of time of sowing.

Resistência de clones de seringueira à infestação por ácaros

Calacarus heveaeFeres (Eriophyidae) and Tenuipalpus heveae Baker (Tenuipalpidae) can cause heavy defoliation of rubber tree plants. From the perspective of integrated pest management, resistant clones can be an important strategy. The objective of this work was to evaluate the resistance of rubber tree clones to the attack of mites, in an experiment carried out in Votuporanga, SP. The experimental design was a randomized blocks with eighteen treatments and three replications. The treatments were the clones PB 230, PB 243, PB 252, PB 294, PB 306, PB 311, PB 312, PB 314, PB 324, PB 346, PB 350, PB 355, IAC 56, IAC 302, IAC 328, IAC 334, Fx 3899 and RRIM 600. The evaluation of C. heveae and T. heveae populations was performed by counting mites and exuviae presence in six leaflets per plot. The defoliation levels were evaluated with a score scale, varying from 0 (no defoliation) to 4 (above 75% of defoliation). Based on the results, Fx 3899 clone is resistant through non-preference and/or antibiosis to C. heveae. The IAC 56 clone is less susceptible to defoliation caused by C. heveae and T. heveae infestation.

Metabolismo oxidativo e cardíaco de equinos submetidos a exercício de baixa intensidade antes e após suplementação com antioxidante

The present study aimed to evaluate cardiac and lipoperoxidation markers in horses subjected to low intensity and long duration (TLD) exercise test, before and after vitamin E supplementation. For this purpose, 10 horses were used, subjecting them to the first TLD with a workload based on individual maximal oxygen uptake (VO2max). Then, horses received vitamin E (dl-alpha-tocopherol) during 59 days at a daily oral dose of 1,000IU, and thereafter they performed a second TLD with the same protocol as the first. Blood samples were collected to determine plasma malondialdehyde (MDA) as an index of lipoperoxidation, serum cardiac troponin I (cTnI) and creatine kinase MB isoenzyme (CK-MB) as cardiac markers. As a result of the exercise, there was no significant increase in MDA or cTnI, but serum CK-MB increased suggesting myocardial stress. The supplementation was able to minimize reactive oxygen species production, as evidenced by lower concentrations of MDA at all times evaluated, but it didn't cause protective effect on the myocardium. It was concluded that the low intensity and long duration exercise promoted light myocardial stress in horses and vitamin E supplementation reduced lipoperoxidation.

Aptidão aeróbia e amplitude dos domínios de intensidade de exercício no ciclismo

INTRODUÇÃO: A determinação dos domínios de intensidade de exercício tem importantes implicações na prescrição do treino aeróbio e na elaboração de delineamentos experimentais. OBJETIVO: Analisar os efeitos do nível de aptidão aeróbia sobre a amplitude dos domínios de intensidade de exercício durante o ciclismo. MÉTODOS: Doze ciclistas (CIC), 11 corredores (COR) e oito indivíduos não treinados (NT) foram submetidos aos seguintes protocolos em diferentes dias: 1) teste progressivo para determinação do limiar de lactato (LL), consumo máximo de oxigênio (VO2máx) e sua respectiva intensidade (IVO2máx); 2) três testes de carga constante até a exaustão a 95, 100 e 110% IVO2máx para a determinação da potência crítica (PC); 3) testes até a exaustão para determinar a intensidade superior do domínio severo (Isup). As amplitudes dos domínios (moderado < LL; LL > pesado < PC; PC > severo < Isup) foram expressas como percentual da Isup (VO2). RESULTADOS: A amplitude do domínio moderado foi similar entre CIC (52 ± 8%) e COR (47 ± 4%) e significantemente maior no CIC em relação ao NT (41 ± 7%). O domínio pesado foi significantemente menor no CIC (17 ± 6%) em relação ao COR (27 ± 6%) e NT (27 ± 9%). Em relação ao domínio severo não foram encontradas diferenças significantes entre os CIC (31 ± 7%), COR (26 ± 5%) e NT (31 ± 7%). CONCLUSÃO: O domínio pesado de exercício é mais sensível a mudanças determinadas pelo nível de aptidão aeróbia, existindo a necessidade de que se atenda ao princípio da especificidade do movimento, quando se pretende obter um elevado grau de adaptação fisiológica.

Ocorrência de pragas na cultura do algodão transgênico (bt) e convencional cultivado no sistema adensado e não-adensado

Pós-graduação em Agronomia - FEIS

Divergência genética de genótipos de feijoeiro a infestação de Zabrotes subfasciatus (Bohemann) (Coleoptera: Bruchidae)

The aim of this work was to evaluate the genetics divergence of bean genotypes in relation to the oviposition, feeding and development of Zabrotes subfasciatus, determining the degrees of resistance to the weevil. The genotypes used were: IAC Carioca-Tybata, IAC Fortaleza, IAPAR 81, IAC Carioca-Ete, IAC Galante, IAC Harmonia, IAC Una, IAC Diplomata, BRS Supremo and RAZ 49. Tests were performed in laboratory under controlled humidity, temperature and photophase conditions. In free choice test, 10 g of bean genotypes seeds were distributed in circular openings placed equidistant from each other in aluminum trays, where 70 couples were released. The attractiveness was evaluated 24 hours and seven days after the experiment started, and then the number of eggs was evaluated. In non choice test, 10 g of seeds were used where seven couples of Z. subfasciatus, 24 hours-old, were released, remaining seven days, and after the adults retreat, the total number of eggs, viable and unviable eggs, the number and percentage of emerged adults, weight, longevity and period from egg to adult of males and females, sex ratio, dry mass and dry mass consumed by insect were evaluated. In the genotype IAC Harmonia was observed the lower oviposition; RAZ 49 was the most non preference-type resistant for feeding and/or antibiosis-type resistant; BRS Supremo, IAC Carioca-Ete and IAPAR 81 are no preference for feeding and/or antibiosis-type moderate resistant; IAC Galante is susceptible and the other genotypes are highly susceptible to Z. subfasciatus.

Pigmentos fotossintéticos e índice spad como descritores de intensidade do estresse por deficiência hídrica em cana-de-açúcar

The objective of this study was to evaluate the responses of sugar cane subject to water stress by photosynthetic pigments (chlorophylls a, b, total a + b, ratio chlorophylls a/b and carotenoids) and verify the use of SPAD index as a cultivar differentiation tool. The experiment was carried out in a greenhouse, where four cultivars (IACSP95-5000, RB835054, RB928064 and SP80-3280) were grown in pots. After 65 days of planting, two treatments were implemented, i.e., with no stress (-D) and with water stress (D +). Cultivars of sugar cane respond differently in relation to photosynthetic pigments when subjected to water deficit. Cultivars IACSP95-5000 and RB928064 have less effect of drought, that is attributed to the ability of maintaining the chlorophyll and carotenoid content, as well as higher SPAD index values under this condition. Water stress affects with more intensity the cultivars RB835054 and SP80-3280 due to higher reductions in photosynthetic pigments and SPAD index. SPAD index is correlated with chlorophyll and carotenoid content in sugar cane and can be used as a technique for selecting tolerant cultivars to drought in breeding programs.

Correlações entre parâmetros aeróbios e desempenho em esforços intermitentes de alta intensidade

The purpose of the study was to examine the relationships between intermittent high-intensity efforts (RAST) parameters and variables related to aerobic metabolism (anaerobic threshold; LAN, maximal oxygen uptake; VO2MAX and velocity correspondent to VO2MAX; iVO(2MAX)). Eight under-17 (U17) soccer players (16 +/- 1 years) participated in the study. The participants were submitted to a graded exercise test and six maximal sprints of 35m with 10 seconds of passive recovery between each effort (RAST). The RAST parameters were not significant correlated with VO2MAX and LAN. However absolute and relative mean power were significantly correlated with iVO(2MAX) (r=0.79 e r=0.85, respectively). Furthermore, the fatigue index and the relative peak power were significantly correlated with the iVO(2MAX) (r=-0,57 e r=0,73, respectively). In conclusion, the only aerobic variable correlated with performance in consecutive efforts with brief recovery periods, such as RAST, is iVO(2MAX).

Treinamento em corrida de baixa intensidade: propriedades estruturais e mecânicas da epífise proximal do fêmur de ratas osteopênicas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Métodos para avaliar a liberação e o parasitismo de Cotesia flavipes (Cameron, 1891) (Hymenoptera: Braconidae) em cana-de-açúcar

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Teatralidade em frequência e intensidade: análise da matriz vocal do espetáculo Passos, do grupo Obragem de Teatro e Cia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Radioterapia de Intensidade Modulada (IMRT): princípios e técnicas

Neste trabalho, o tema central será a Radioterapia de Intensidade Modulada (IMRT). Quanto a metodologia, trata-se fundamentalmente de uma pesquisa bibliográfica somada ao acompanhamento da rotina da IMRT no Hospital Sírio Libanês e do acompanhamento do controle de qualidade do planejamento com o equipamento MapCHECK no Hospital A. C. Camargo. Os principais objetivos deste trabalho são: descrever a técnica, os seus princípios de funcionamento, os testes de aceite, o comissionamento, o controle de qualidade e a rotina clínica. Para desta maneira, avaliar a IMRT, a sua viabilidade e a importância do físico e da equipe multiprofissional no processo de implementação

Respostas da frequência de braçada e do comprimento de braçada na e acima da intensidade correspondente à máxima fase estável de lactato sanguíneo em indivíduos com diferentes níveis de performance

The main objective of this study was to analyze the behavior of variables related to swim ability at and above maximal lactate steady state (MLSS), performed at continuous and intermittent conditions in individuals with different aerobic performance levels. Participated of this study male swimmers with ages between 20 to 25 years, specialists in events of 400 m, 800 m and 1500 m and open water swims, with at least 3 years of experience in the modality. The individuals performed a maximal 400-m swim test. After this test, they were divided into two groups, in accordance with the speed attained during 400-m swim test: G1 (higher performance) and G2 (lower performance). For the determination of continuous MLSS (MLSSc), 2 to 4 trials of 30-min were performed. For the determination of the intermittent MLSS (MLSSi) 2 to 4 trials of 30-min (12 repetitions of 2 min 30 s, with 30 s of rest) were performed, in constant speed, with the first trial performed at 102.5% MLSSc. Th technical indexes, stroke rate (FB) and stroke length (CB) were determined in all tests. The SR was calculated trough recordings using the time needed to perform five stroke cycles. The SL was calculated dividing the speed by the SR. There was no significant difference on the antropometric characteristics between groups. The speed at and above MLSSc were significantly higher at G1 (1,23±0,05 e 1,27±0,06, respectively) than G2 (1,10±0,06 e 1,13±0,06, respectively). There was significant change in SL and SR in G2. In the same way, there was significant change in SL and SR only in G2, above MLSSc. Similar to continuous condition, the speeds at and above MLSSi were significantly higher in G1 (1,27±0,05 e 1,30±0,05, respectively) do que no G2 (1,14±0,07 e 1,16±0,07, respectively). There was significant change in SL and SR only in G2. There was significant change in SR and SL in both groups above MLSSi. Thus,...(Complete abstract click electronic access below)

Estudo da variação da porcentagem e da estimativa de geração de gás metano para o aterro sanitário do município de Rio Claro - SP

Increased demand for energy and the search for alternative energy sources are remarkable and current facts. The management of domestic solid waste is also part of the issue in view of the possibility of power generation in existing landfills, which could provide financial autonomy to the system of waste management and improve the environmental balance of landfills in Brazil, currently only used for final solids disposal. Landfill gas is a viable alternative energy source has been tested in landfills in the World and in Brazil. Thus, the research aimed to evaluate the percentage of methane (CH4) present in three sinks of gases from a landfill in the municipality of Rio Claro – SP (Brazil), and to estimate the potential methane generation over its life through the application of a mathematical model. As a result it was found that in general the percentage of methane present in the gases is significant, between 50 and 62%. It was also noted that waste with disposal time between 8 and 12 months already are in the methanogenic stage. Finally, there was a tendency to increase the generation of methane in the transition periods of rain and drought