Se nas pedras faz feguras parecer... Consideraciones sobre iconografía mariana y figuras poéticas en las Cantigas de Santa María de Alfonso X

La iconografía mariana ha quedado reflejada en la poesía hispánica medieval de muchas maneras: desde versos que describen a la Virgen y sus atributos hasta conceptos teológicos y mariológicos expresados plásticamente mediante formas retóricas. Por ejemplo, el jardín y las flores como ornamentos y símbolos personales de Santa María, o bien la imagen de la luz a través de un cristal o una ventana de vidrio en escenas de la Anunciación -con abundantes testimonios pictóricos en el arte medieval-, se encuentran en la poesía en forma de descripciones simbólicas de diversa extensión y género y, por otro lado, de advocaciones marianas o tópicos mariológicos (Ave/Eva, Flos, Hortus, Radix-Virga, Regina, Stella). Este artículo propone, pues, un estudio de conjunto y comparativo de algunos de estos procedimientos, concentrándose en las Cantigas de Santa María del rey Alfonso X. En este sentido, las figuras retóricas, como herramientas que tienden un puente entre lo pictórico y lo poético, dirigen necesariamente la lectura hacia una interpretación simbólica proporcionada por la figuración o typologia, tal como la ha postulado Erich Auerbach para la textualidad medieval. En última instancia, el valor sagrado de la imagen mariana (una herencia del arte icónico bizantino) se representa también en los milagros de las Cantigas de Santa María, obra maestra que evidencia una fuerte influencia de la doctrina iconodúlica.

Corteza y gravedad: el plano del suelo como estrategia en la arquitectura contemporánea

Es, en el encuentro de los edificios con el terreno, donde el suelo como realidad se transforma en cualidad arquitectónica. La presente tesis aborda el estudio del plano del suelo, haciendo una revisión crítica de la arquitectura como mecanismo de pensamiento proyectual. Este análisis se enmarca a partir de los años sesenta, por considerar que es cuando comienza a evidenciarse la ruptura respecto a la herencia del Movimiento Moderno. Es entonces cuando la arquitectura marca un punto de inflexión, y empiezan a surgir diferentes actitudes metodológicas respecto al suelo, totalmente nuevas. Las clásicas acciones de encuentro como posar, elevar o enterrar fueron poco a poco sustituidas por otras más complejas como plegar, inclinar o esponjar. Utilizando como marco de restricción los encuentros o desencuentros del objeto arquitectónico con el terreno, se analiza el suelo como estrategia arquitectónica tratando de demostrar como su manipulación puede ser una eficaz herramienta con la que establecer relaciones específicas con el lugar. La capacidad que presenta el suelo, como elemento arquitectónico, de explorar y modificar las características de cada entorno, hacen de esta superficie una eficiente forma de contextualización. Por tanto, la manipulación del suelo que aquí se plantea, opera transcodificando los elementos específicos de cada lugar y actúa como estrategia arquitectónica que pone en relación al edificio con el contexto, modificando las particularidades formales de dicho plano. Frente a la tendencia que reduce la expresión arquitectónica a una simple apariencia formal autónoma, se plantea la manipulación del plano del suelo como mecanismo de enraizamiento con el lugar, enfatizando para ello la condición terrestre de la arquitectura. El plano del suelo es el que ata al edificio mediante la gravedad a la corteza terrestre. En realidad se trata de realzar el carácter mediador del suelo en la arquitectura, definiendo para ello el establecimiento de elementos comunes entre realidades distintas, potenciando el valor del suelo como herramienta que puede transcodificar el entorno, trasformando su datos en elementos arquitectónicos concretos. En este proceso de traducción de información, el suelo pasa de ser un objeto pasivo a ser uno operativo, convirtiéndose en parte activa de las acciones que sobre él se ejercen. La tesis tiene también como propósito demostrar cómo, la clave de la rápida evolución que el suelo como estrategia arquitectónica ha sufrido en los últimos años, mucho debe a la expansión del suelo en otras artes como en la escultura, y mas concretamente en el landart. Surgen entonces nuevas disciplinas, en las que se propone la comprensión del lugar en los proyectos desarrollando una visión integral del mundo natural, convirtiéndolo en un tejido viviente y conector que pone en relación las actividades que sustenta. También encontramos en Utzon, y sus plataformas geológicas, al precursor de la importancia que más tarde se le daría al plano del suelo en la arquitectura, ya que inicia cierta actitud crítica, que hizo avanzar hacia una arquitectura más expresiva que requería nuevos mecanismos que la relacionasen con el suelo que los soportaba, proponiendo con sus plataformas una transformación infraestructural del suelo. Con su interpretación transcultural de las estructuras espaciales arquetípicas mayas, chinas y japonesas, irá enriqueciendo el panorama arquitectónico, adquiriendo de este modo más valor el contexto que acabará por ser entendido de forma más compleja. Los proyectos de arquitectura en muchas ocasiones se han convertido en territorios propicios de especulación donde construir teoría arquitectónica. Desde este contexto se analizan cuatro estrategias de suelo a través del estudio de cuatro posiciones arquitectónicas muy significativas desde el punto de vista de la manipulación del plano del suelo, que construyen una interesante metodología proyectual con la que operar. Los casos de estudio, propuestos son; la Terminal Pasajeros (1996-2002) en Yokohama del estudio FOA, la Casa de la Música (1999-2005) de OMA en Oporto, el Memorial Judío (1998-2005) de Berlín de Peter Eisenman, y por último el Museo MAXXI (1998-2009) de Zaha Hadid en Roma. Descubrir las reglas, referencias y metodologías que cada uno nos propone, nos permite descubrir cuáles son los principales posicionamientos en relación al proyecto y su relación con el lugar. Las propuestas aquí expuestas abordan una nueva forma de entender el suelo, que hizo avanzar a la arquitectura hacia nuevos modos de encuentro con el terreno. Nos permite también establecer cuáles son las principales aportaciones arquitectónicas del suelo, como estrategia arquitectónica, que han derivado en su reformulación. Dichas contribuciones abren nuevas formas de abordar la arquitectura basadas en el movimiento y en la flexibilidad funcional, o en la superposición de flujos de información y circulación. También plantean nuevas vías desdibujando la figura contra el fondo, y refuerzan la idea del suelo como plataforma infraestructural que ya había sido enunciada por Utzon. Se trata en definitiva de proponer la exploración de la superficie del suelo como el elemento más revelador de las formas emergentes del espacio. ABSTRACT Where the building hits the ground, it is where the latter as reality becomes architectural quality. This thesis presents the study of the ground plane, making a critical review of architecture as a mechanism of projectual thought. This analysis starts from the sixties, because it is when the break begins to be demonstrated with regard to the inheritance of the Modern Movement. It is then when architecture marks a point of inflexion, and different, completely new methodological attitudes to the ground start to emerge. The classic meeting action like place, raise or bury are gradually replaced by more complex operations such as fold, bend or fluff up. Framing it within the meetings or disagreements between architectural object and ground, we analyzed it as architectural strategy trying to show how handling can be an effective tool to establish a specific relationship with the place. The capacity ground has, as an architectural element, to explore and modify the characteristics of each environment, makes this area an efficient tool for contextualization. Therefore, the manipulation of ground that is analyzed here, operates transcoding the specifics of each place and acts as architectural strategy that relates to the building with the context, modifying the structural peculiarities of such plane. Opposite to the reductive tendency of the architectural expression to a simple formal autonomous appearance, the manipulation of the ground plane is considered as a rooting mechanism place that emphasises the earthly condition of architecture. The ground plane is the one that binds the building by gravity to the earth’s crust. In fact, it tries to study the mediating character of the ground in architecture, defining for it to establish commonalities among different realities, enhancing the value of the ground as a tool that can transcode the environment, transforming its data into specific architectural elements. In this process of translating information, the ground goes from being a liability, to become active part of the actions exerted on the object. The thesis also tries to demonstrate how the key of the rapid evolution that the ground likes architectural strategy has gone through recently, much due to its use expansion in other arts such as sculpture. New disciplines arise then, in which one proposes the local understanding in the projects, developing an integral vision of the natural world and turning it into an element linking the activities it supports. We also find in Utzon, and his geological platforms, the precursor of the importance that later would be given to the ground plane in architecture, since it initiates a certain critical attitude, which advances towards a more expressive architecture, with new mechanisms that relate to the ground that it sits in, proposing with its platforms an infrastructural transformation of the ground. With his transcultural interpretation of the spatial archetypal structures, he will enrich the architectural discourse, making the context become understood in more complex ways. Architectural projects in many cases have become territories prone to architectural theory speculation. Within this context, four strategies are analyzed through the study of four very significant architectural positions, from the point of view of handling the ground plane, and the project methodology within which to operate. The case studies analyzed are; Passenger Terminal (1996-2002) in Yokohama from FOA, The House of the music (1999-2005) the OMA in Oporto, The Jew monument (1998-2005) in Berlin the Peter Eisenman, and finally the MAXXI Museum (1998-2009) the Zaha Hadid in Rome. Discovering the rules, references and methodologies that each of those offer, it allows to discover what the main positions are regarding the building and its relationship with the place where it is located. The proposals exposed here try to shed a different light on the ground, which architecture advancing in new ways on how meet it. The crossing of the different studied positions, allows us to establish what the main contributions of ground as architectural strategy are. Such contributions open up new approaches to architecture based on movement and functional flexibility, overlapping information flow and circulation, consider new ways that blur the figure against the background, and reinforce the idea of ground as infrastructural platform, already raised by Utzon. Summarizing, it tries to propose the exploration of the ground plane as the most revealing form of spatial exploration.

A EXPECTATIVA DOS PAIS DIANTE DA EDUCAÇÃO INFANTIL: UM ESTUDO DE CASO

A partir do estudo de caso de uma unidade privada de educação infantil, a presente pesquisa pretendeu investigar, através das expectativas dos pais diante da educação infantil, a influência da herança cultural familiar na trajetória escolar da criança. O interesse teórico de uma pesquisa empírica sobre esse universo é atestado pelo fato de que, conforme Pierre Bourdieu, a valorização e a compreensão da escola, já nos primeiros anos de vida da criança são comuns entre as famílias que possuem um maior nível de escolarização e que conseqüentemente começam a traçar desde cedo a trajetória escolar de seus filhos. Assim, ao estudarmos os pais de alunos de uma unidade privada de educação infantil, estaremos abordando, sobretudo, as expectativas educacionais de famílias da classe média. Apoiados em algumas idéias básicas sobre a conexão entre capital cultural e estratégias educacionais apresentados por Bourdieu, trabalhamos com a hipótese de que as diferentes categorias sociais são desigualmente predispostas a compreender e a valorizar a escolarização em geral, e que este fato está diretamente relacionado ao capital cultural familiar. De acordo com o autor é o volume e o tipo de capital (econômico, social e cultural) que o indivíduo possui que irá definir sua posição na hierarquia social, bem como suas expectativas diante da escola. Nota-se, então, que a cultura de um modo geral opera como um patrimônio de diferenciação de classe.(AU)

A EXPECTATIVA DOS PAIS DIANTE DA EDUCAÇÃO INFANTIL: UM ESTUDO DE CASO

A partir do estudo de caso de uma unidade privada de educação infantil, a presente pesquisa pretendeu investigar, através das expectativas dos pais diante da educação infantil, a influência da herança cultural familiar na trajetória escolar da criança. O interesse teórico de uma pesquisa empírica sobre esse universo é atestado pelo fato de que, conforme Pierre Bourdieu, a valorização e a compreensão da escola, já nos primeiros anos de vida da criança são comuns entre as famílias que possuem um maior nível de escolarização e que conseqüentemente começam a traçar desde cedo a trajetória escolar de seus filhos. Assim, ao estudarmos os pais de alunos de uma unidade privada de educação infantil, estaremos abordando, sobretudo, as expectativas educacionais de famílias da classe média. Apoiados em algumas idéias básicas sobre a conexão entre capital cultural e estratégias educacionais apresentados por Bourdieu, trabalhamos com a hipótese de que as diferentes categorias sociais são desigualmente predispostas a compreender e a valorizar a escolarização em geral, e que este fato está diretamente relacionado ao capital cultural familiar. De acordo com o autor é o volume e o tipo de capital (econômico, social e cultural) que o indivíduo possui que irá definir sua posição na hierarquia social, bem como suas expectativas diante da escola. Nota-se, então, que a cultura de um modo geral opera como um patrimônio de diferenciação de classe.(AU)

Defective placental vasculogenesis causes embryonic lethality in VHL-deficient mice

Inheritance of an inactivated form of the VHL tumor suppressor gene predisposes patients to develop von Hippel–Lindau disease, and somatic VHL inactivation is an early genetic event leading to the development of sporadic renal cell carcinoma. The VHL gene was disrupted by targeted homologous recombination in murine embryonic stem cells, and a mouse line containing an inactivated VHL allele was generated. While heterozygous VHL (+/−) mice appeared phenotypically normal, VHL −/− mice died in utero at 10.5 to 12.5 days of gestation (E10.5 to E12.5). Homozygous VHL −/− embryos appeared to develop normally until E9.5 to E10.5, when placental dysgenesis developed. Embryonic vasculogenesis of the placenta failed to occur in VHL −/− mice, and hemorrhagic lesions developed in the placenta. Subsequent hemorrhage in VHL −/− embryos caused necrosis and death. These results indicate that VHL expression is critical for normal extraembryonic vascular development.

Chemical phenotype matching between a plant and its insect herbivore

Two potential outcomes of a coevolutionary interaction are an escalating arms race and stable cycling. The general expectation has been that arms races predominate in cases of polygenic inheritance of resistance traits and permanent cycling predominates in cases in which resistance is controlled by major genes. In the interaction between Depressaria pastinacella, the parsnip webworm, and Pastinaca sativa, the wild parsnip, traits for plant resistance to insect herbivory (production of defensive furanocoumarins) as well as traits for herbivore “virulence” (ability to metabolize furanocoumarins) are characterized by continuous heritable variation. Furanocoumarin production in plants and rates of metabolism in insects were compared among four midwestern populations; these traits then were classified into four clusters describing multitrait phenotypes occurring in all or most of the populations. When the frequency of plant phenotypes belonging to each of the clusters is compared with the frequency of the insect phenotypes in each of the clusters across populations, a remarkable degree of frequency matching is revealed in three of the populations. That frequencies of phenotypes vary among populations is consistent with the fact that spatial variation occurs in the temporal cycling of phenotypes; such processes contribute in generating a geographic mosaic in this coevolutionary interaction on the landscape scale. Comparisons of contemporary plant phenotype distributions with phenotypes of herbarium specimens collected 9–125 years ago from across a similar latitudinal gradient, however, suggest that for at least one resistance trait—sphondin concentration—interactions with webworms have led to escalatory change.

Somatic and germ cell parasitism in a colonial ascidian: Possible role for a highly polymorphic allorecognition system

A colonial protochordate, Botryllus schlosseri, undergoes a natural transplantation reaction in the wild that results alternatively in colony fusion (chimera formation) or inflammatory rejection. A single, highly polymorphic histocompatibility locus (called Fu/HC) is responsible for rejection versus fusion. Gonads are seeded and gametogenesis can occur in colonies well after fusion, and involves circulating germ-line progenitors. Buss proposed that colonial organisms might develop self/non-self histocompatibility systems to limit the possibility of interindividual germ cell “parasitism” (GCP) to histocompatible kin [Buss, L. W. (1982) Proc. Natl. Acad. Sci. USA 79, 5337–5341 and Buss, L. W. (1987) The Evolution of Individuality (Princeton Univ. Press, Princeton]. Here we demonstrate in laboratory and field experiments that both somatic cell and (more importantly) germ-line parasitism are a common occurrence in fused chimeras. These experiments support the tenet in Buss’s hypothesis that germ cell and somatic cell parasitism can occur in fused chimeras and that a somatic appearance may mask the winner of a gametic war. They also provide an interesting challenge to develop formulas that describe the inheritance of competing germ lines rather than competing individuals. The fact that fused B. schlosseri have higher rates of GCP than unfused colonies additionally provides a rational explanation for the generation and maintenance of a high degree of Fu/HC polymorphism, largely limiting GCP to sibling offspring.

Delayed symptom onset and increased life expectancy in Sandhoff disease mice treated with N-butyldeoxynojirimycin

Sandhoff disease is a neurodegenerative disorder resulting from the autosomal recessive inheritance of mutations in the HEXB gene, which encodes the β-subunit of β-hexosaminidase. GM2 ganglioside fails to be degraded and accumulates within lysosomes in cells of the periphery and the central nervous system (CNS). There are currently no therapies for the glycosphingolipid lysosomal storage diseases that involve CNS pathology, including the GM2 gangliosidoses. One strategy for treating this and related diseases is substrate deprivation. This would utilize an inhibitor of glycosphingolipid biosynthesis to balance synthesis with the impaired rate of catabolism, thus preventing storage. One such inhibitor is N-butyldeoxynojirimycin, which currently is in clinical trials for the potential treatment of type 1 Gaucher disease, a related disease that involves glycosphingolipid storage in peripheral tissues, but not in the CNS. In this study, we have evaluated whether this drug also could be applied to the treatment of diseases with CNS storage and pathology. We therefore have treated a mouse model of Sandhoff disease with the inhibitor N-butyldeoxynojirimycin. The treated mice have delayed symptom onset, reduced storage in the brain and peripheral tissues, and increased life expectancy. Substrate deprivation therefore offers a potentially general therapy for this family of lysosomal storage diseases, including those with CNS disease.

Roles for genomic imprinting and the zygotic genome in placental development

The placenta contains several types of feto-maternal interfaces where zygote-derived cells interact with maternal cells or maternal blood for the promotion of fetal growth and viability. The genetic factors regulating the interactions between different cell types within feto-maternal interfaces and the relative contributions of the maternal and zygotic genomes are poorly understood. Genomic imprinting, the epigenetic process responsible for parental origin-dependent functional differences between homologous chromosomes, has been proposed to contribute to these events. Previous studies showed that mouse conceptuses with an absence of imprinted differences between the two copies of chromosome 12 (upon paternal inheritance of both copies) die late in gestation and have a variety of defects, including placentomegaly. Here we examined the role of chromosome 12 imprinting in these placentae in more detail. We show that the spatial interactions between different cell types within feto-maternal interfaces are defective and identify abnormal behaviors in both zygote-derived and maternal cells that are attributed to the genome of the zygote but not the mother. These include compromised invasion of the maternal decidualized endometrium and the central maternal artery situated within it by zygote-derived trophoblast, abnormalities in the wall of the central maternal artery, and defects within the zygote-derived cellular layer of the labyrinth, which is in direct contact with maternal blood. These findings demonstrate multiple roles for chromosome 12 imprinting in the placenta that have not previously been associated with imprinting effects. They provide insights into the function of imprinting in placental development and have evolutionary and clinical implications.

Symmetries throughout organic evolution

The biological realm has inherited symmetries from the physicochemical realm, but with the increasing complexity at higher phenomenological levels of life, some inherited symmetries are broken while novel symmetries appear. These symmetries are of two types, structural and operational. Biological novelties result from breaking operational symmetries. They are followed by acquisition of regularity and stability, in a recurrent process throughout complexity levels.

A mutation that allows endosperm development without fertilization.

The mechanisms that initiate reproductive development after fertilization are not understood. Reproduction in higher plants is unique because it is initiated by two fertilization events in the haploid female gametophyte. One sperm nucleus fertilizes the egg to form the embryo. A second sperm nucleus fertilizes the central cell to form the endosperm, a unique tissue that supports the growth of the embryo. Fertilization also activates maternal tissue differentiation, the ovule integuments form the seed coat, and the ovary forms the fruit. To investigate mechanisms that initiate reproductive development, a female-gametophytic mutation termed fie (fertilization-independent endosperm) has been isolated in Arabidopsis. The fie mutation specifically affects the central cell, allowing for replication of the central cell nucleus and endosperm development without fertilization. The fie mutation does not appear to affect the egg cell, suggesting that the processes that control the initiation of embryogenesis and endosperm development are different. FIE/fie seed coat and fruit undergo fertilization-independent differentiation, which shows that the fie female gametophyte is the source of signals that activates sporophytic fruit and seed coat development. The mutant fie allele is not transmitted by the female gametophyte. Inheritance of the mutant fie allele by the female gametophyte results in embryo abortion, even when the pollen bears the wild-type FIE allele. Thus, FIE carries out a novel, essential function for female reproductive development.

Fluorescent light-induced chromatid breaks distinguish Alzheimer disease cells from normal cells in tissue culture.

The neurodegeneration and amyloid deposition of sporadic Alzheimer disease (AD) also occur in familial AD and in all trisomy-21 Down syndrome (DS) patients, suggesting a common pathogenetic mechanism. We investigated whether defective processing of damaged DNA might be that mechanism, as postulated for the neurodegeneration in xeroderma pigmentosum, a disease with defective repair not only of UV radiation-induced, but also of some oxygen free radical-induced, DNA lesions. We irradiated AD and DS skin fibroblasts or blood lymphocytes with fluorescent light, which is known to cause free radical-induced DNA damage. The cells were then treated with either beta-cytosine arabinoside (araC) or caffeine, and chromatid breaks were quantified. At least 28 of 31 normal donors and 10 of 11 donors with nonamyloid neurodegenerations gave normal test results. All 12 DS, 11 sporadic AD, and 16 familial AD patients tested had abnormal araC and caffeine tests, as did XP-A cells. In one of our four AD families, an abnormal caffeine test was found in all 10 afflicted individuals (including 3 asymptomatic when their skin biopsies were obtained) and in 8 of 11 offspring at a 50% risk for AD. Our tests could prove useful in predicting inheritance of familial AD and in supporting, or rendering unlikely, the diagnosis of sporadic AD in patients suspected of having the disease.

GSP-1 genes are linked to the grain hardness locus (Ha) on wheat chromosome 5D.

An important determinant of wheat grain quality is the hardness of the grain. The trait is controlled by a major locus, Ha, on the short arm of chromosome 5D. Purified starch granules from soft-grained wheats have associated with them 15-kDa polypeptides called grain softness proteins (GSPs) or "friabilins." Genes that encode one family of closely related GSP polypeptides - GSP-1 genes - were mapped using chromosome substitution lines to the group 5 chromosomes. An F2 population segregating for hard and soft alleles at the Ha locus on a near-isogenic background was used in a single-seed study of the inheritance of grain softness and of GSP-1 alleles. Grain softness versus grain hardness was inherited in a 3:1 ratio. The presence versus absence of GSPs in single seed starch preparations was coinherited with grain softness versus hardness. This showed that grain softness is primarily determined by seed, and not by maternal, genotype. In addition, no recombination was detected in 44 F2 plants between GSP-1 restriction fragment length polymorphisms and Ha alleles. Differences between hard and soft wheat grains in membrane structure and lipid extractability have been described and, of the three characterized proteins that are part of the mixture of 15-kDa polypeptides called GSPs, at least two, and probably all three, are proteins that bind polar lipids. The data are interpreted to suggest that the Ha locus may encode one or more members of a large family of lipid-binding proteins.

Extending the chemistry that supports genetic information transfer in vivo: phosphorothioate DNA, phosphorothioate RNA, 2'-O-methyl RNA, and methylphosphonate DNA.

DNA and RNA are the polynucleotides known to carry genetic information in life. Chemical variants of DNA and RNA backbones have been used in structure-function and biosynthesis studies in vitro, and in antisense pharmacology, where their properties of nuclease resistance and enhanced cellular uptake are important. This study addressed the question of whether the base(s) attached to artificial backbones encodes genetic information that can be transferred in vivo. Oligonucleotides containing chemical variants of DNA or RNA were used as primers for site-specific mutagenesis of bacteriophage f1. Progeny phage were scored both genetically and physically for the inheritance of information originally encoded by bases attached to the nonstandard backbones. Four artificial backbone chemistries were tested: phosphorothioate DNA, phosphorothioate RNA, 2'-O-methyl RNA and methylphosphonate DNA. All four were found capable of faithful information transfer from their attached bases when one or three artificial positions were flanked by normal DNA. Among oligonucleotides composed entirely of nonstandard backbones, only phosphorothioate DNA supported genetic information transfer in vivo.

A cytoplasmically transmissible hypovirulence phenotype associated with mitochondrial DNA mutations in the chestnut blight fungus Cryphonectria parasitica.

Mutations causing mitochondrial defects were induced in a virulent strain of the chestnut blight fungus Cryphonectria parasitica (Murr.) Barr. Virulence on apples and chestnut trees was reduced in four of six extensively characterized mutants. Relative to the virulent progenitor, the attenuated mutants had reduced growth rates, abnormal colony morphologies, and few asexual spores, and they resembled virus-infected strains. The respiratory defects and attenuated virulence phenotypes (hypovirulence) were transmitted from two mutants to a virulent strain by hyphal contact. The infectious transmission of hypovirulence occurred independently of the transfer of nuclei, did not involve a virus, and dynamically reflects fungal diseases caused by mitochondrial mutations. In these mutants, mitochondrial mutations are further implicated in generation of the attenuated state by (i) uniparental (maternal) inheritance of the trait, (ii) presence of high levels of cyanide-insensitive mitochondrial alternative oxidase activity, (iii) cytochrome deficiencies, and (iv) structural abnormalities in the mtDNA. Hence, cytoplasmically transmissible hypovirulence phenotypes found in virus-free strains of C. parasitica from recovering trees may be caused by mutant forms of mtDNA.