EXOTIC CARBON SYSTEMS IN TWO-NEUTRON HALO THREE-BODY MODELS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Repeatability of corrosion parameters for titanium-molybdenum alloys in 0.9% NaCl solution

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The generation and utilization of a cancer-oriented representation of the human transcriptome by using expressed sequence tags

Whereas genome sequencing defines the genetic potential of an organism, transcript sequencing defines the utilization of this potential and links the genome with most areas of biology. To exploit the information within the human genome in the fight against cancer, we have deposited some two million expressed sequence tags (ESTs) from human tumors and their corresponding normal tissues in the public databases. The data currently define approximate to23,500 genes, of which only approximate to1,250 are still represented only by ESTs. Examination of the EST coverage of known cancer-related (CR) genes reveals that <1% do not have corresponding ESTs, indicating that the representation of genes associated with commonly studied tumors is high. The careful recording of the origin of all ESTs we have produced has enabled detailed definition of where the genes they represent are expressed in the human body. More than 100,000 ESTs are available for seven tissues, indicating a surprising variability of gene usage that has led to the discovery of a significant number of genes with restricted expression, and that may thus be therapeutically useful. The ESTs also reveal novel nonsynonymous germline variants (although the one-pass nature of the data necessitates careful validation) and many alternatively spliced transcripts. Although widely exploited by the scientific community, vindicating our totally open source policy, the EST data generated still provide extensive information that remains to be systematically explored, and that may further facilitate progress toward both the understanding and treatment of human cancers.

In vitro analysis with human bone marrow stem cells on Ti-15Mo alloy for dental and orthopedic implants application

Aim: Nowadays, research on orthopedic and dental implants is focused on titanium alloys for their mechanical properties and corrosion resistance in the human body environment. Another important aspect to be investigated is their surface topography, which is very important to osseointegration. With laser beam irradiation for roughening the implants surface an easier control of the microtopography is achieved, and surface contamination is avoided. The aim of this study was to assess human bone marrow stem cells response to a newly developed titanium alloy, Ti-15Mo, with surface topography modified by laser beam irradiation. Materials and methods: A total of 10 Ti machined disks (control), 10 Ti-15Mo machined disks and 10 Ti-15Mo disks treated by laser beam-irradiation were prepared. To study how Ti-15Mo surface topografy can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were analyzed, using real time Reverse Transcription-Polymerase Chain Reaction. Results: In Test 1 (comparison between Ti-15Mo machined disks and Ti-machined disks) quantitative real-time RT-PCR showed a significant induction of ALPL, FOSL1 and SPP1, which increase 20% or more. In Test 2 (comparison between Ti-15Mo laser treated disks and Ti-machined disks) all investigated genes were up-regulated. By comparing Test 1 and Test 2 it was detected that COL1A1, COL3A1, FOSL1 and ENG sensibly increased their expression whereas RUNX2, ALPL and SPP1 expression remained substantially unchanged. Conclusion: The present study demonstrated that laser treated Ti-15Mo alloys are promising materials for implants application.

In Situ Formation of Dendrites in Eumelanin Thin Films between Gold Electrodes

Eumelanin is a ubiquitous pigment in the human body, animals, and plants, with potential for bioelectronic applications because of its unique set of physical and chemical properties, including strong UV-vis absorption, mixed ionic/electronic conduction, free radical scavenging and anti-oxidant properties. Herein, a detailed investigation is reported of eumelanin thin films grown on substrates patterned with gold electrodes as a model system for device integration, using electrical measurements, atomic force microscopy, scanning electron microscopy, fluorescence microscopy, and time-of-flight secondary ion mass spectroscopy. Under prolonged electrical biasing in humid air, one can observe gold dissolution and formation of gold-eumelanin nanoaggregates, the assembly of which leads to the formation of dendrites forming conductive pathways between the electrodes. Based on results collected with eumelanins from different sources, a mechanism is proposed for the formation of the nanoaggregates and dendrites, taking into account the metal binding properties of eumelanin. The surprising interaction between eumelanin and gold points to new opportunities for the fabrication of eumelanin-gold nanostructures and biocompatible memory devices and should be taken into account in the design of devices based on eumelanin thin films. © 2013 WILEY-VCH Verlag GmbH & Co.

Tissue engineering: Using collagen type i matrix for bone healing of bone defects

Among the many tissues in the human body, bone has been considered as a powerful marker for regeneration and its formation serves as a prototype model for tissue engineering based on morphogenesis. Therefore, collagen type I is one of the most useful biomaterials used in tissue engineering as extracellular matrix components capable to promote bone healing. The literature reveals excellent biocompatibility and safety due to its biological characteristics, such as biodegradability and weak antigenicity, making collagen type I the primary resource in medical applications. Thus, it was also used for tissue engineering including skin replacement, bone substitutes, and artificial blood vessels and valves. The authors describe the treatment of an abscessed apical periodontal cyst and show good outcomes of bone healing, using tissue engineering, as collagen type I matrix. © 2013 by Mutaz B. Habal, MD.

Continuum psicofísico: uma abordagem baseada no pensamento de Charles S. Pierce

Pós-graduação em Filosofia - FFC

Sistema de coleta, análise e detecção de código malicioso baseado no sistema imunológico humano

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Uma aproximação didática por meio da história do conceito de circulação sanguínea

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O laboratório de anatomia sob a perspectiva da 'descrição densa': interfaces da cultura e o ensino de Ciências

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo de um modelo dinâmico para avaliação física do corpo humano

Pós-graduação em Engenharia Mecânica - FEG

Representação social de ciência de estudantes do ensino fundamental da rede municipal de Belém

Este trabalho relata uma pesquisa descritiva quanti-qualitativa realizada entre estudantes do ensino fundamental da rede municipal de Belém, com os objetivos de identificar a representação social de Ciência desses alunos, além de compreender os conteúdos e identificar as tendências de núcleo central reveladas nessas representações. A pesquisa teve como sujeitos 124 estudantes de uma escola da rede municipal de Belém, dos quais 65 encontravam-se cursando 3o ciclo e 59 o 4o ciclo do ensino fundamental. Dos 124, 52 são do sexo masculino e 72 são do sexo feminino, distribuídos entre as faixas etárias de 10 a 17 anos. Tais representações são discutidas tendo como referências teórico-metodológicas a teoria das representações sociais de Serge Moscovici, e suas teorias complementares, como a Teoria do Núcleo Central de Jean-Claude Abric. Os dados coletados através de questionários, compostos por uma questão aberta e uma de evocação livre foram analisados utilizando-se a técnica de análise de conteúdo e de Vergès, respectivamente. A análise dos dados nos revelou a vinculação restrita da Ciência à disciplina escolar Ciências e a alguns de seus conteúdos, como corpo humano e meio ambiente, entre outros. Esses resultados nos fazem concluir que tais representações estão fortemente vinculadas à abordagem adotada pelo ensino de Ciências, ainda predominantemente prescritivo e propedêutico. Com essa conclusão evidencia-se a necessidade de incentivo à formação dos professores de Ciências, na expectativa de se viabilizar a implementação de propostas de ensino que pretendam romper, com esse modelo tradicional, como Alfabetização Científica e Ciência, Tecnologia e Sociedade (CTS), dessa forma contribuindo para formação de cidadãos atuantes de maneira crítica e criativa em nossa sociedade.

Atividades investigativas, como estratégia para o ensino-aprendizagem em ciências: propostas e aprendizagens

Partindo da premissa que O ensinar requer saber aprender e saber construir (juntamente com os nossos alunos) as aprendizagens que buscamos, desenvolvi uma proposta de atividades investigativas, para o ensino-aprendizagem em Ciências. Ressalto que ministrar aulas diferenciadas, nem sempre seria ou é fácil, pois há um conteúdo a ser cumprido e terminado, além de um tempo muito curto para realizá-las. Assim, minha dissertação é uma investigação do tipo qualitativa, que se configura como uma pesquisa-ação, sobre as atividades programadas em momentos, com alunos das 7 séries do ensino fundamental de duas escolas da rede particular de ensino do município de Belém (denominadas aqui, Escola A e Escola B), tendo como conteúdo programático o corpo humano. Na escola A, enfatizando o sistema digestório, tivemos os seguintes momentos: o grande lanche; um vídeo sobre o processo de digestão alimentar e mini-gincana (prova). Já na escola B, com o conteúdo sobre o Sistema Locomotor, os momentos: construção hipotética do corpo humano, a partir do contorno de um modelo; a caminhada até uma Academia de Ginástica com a realização de atividades; aulas na quadra da escola, em conjunto com o professor de Ed. Física; e por fim, a confecção de um CD-ROM com todos os assuntos trabalhados, durante o ano letivo. Em ambas as escolas, houve o momento da releitura dos livros didáticos. Concluímos que aprendizagens, a partir de atividades investigativas, e que levam em conta as vivências, os questionamentos e as contribuições dos alunos, são efetivas, independente do assunto que será abordado e da Instituição de Ensino (pública ou particular). Porém, consideramos que essa metodologia de trabalho funcionará se o professor: 1 estiver disposto a continuar seus estudos e 2 compartilhar com seus alunos os sabores e os saberes, assim como os ensinamentos e as aprendizagens.

Dinâmica do aparelho locomotor em sujeitos amputados e não-amputados do membro inferior

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Dynamics of the interaction between body fluid and Ti cp: the influence of surface functionalization in the first stages of osseointegration

INTRODUCTION: Regenerative therapies using biomaterials require accurate information on interactions between the implanted material and the human body. To improve the process of bone regeneration it is necessary to obtain a better understanding of the influence of the surfaces on the early stages of osseointegration. This work aims to investigate the dynamic interaction between simulated body fluid (SBF) and titanium surfaces (Ti cp) immediately after their first contact. METHODS: Ti cp samples were passed through physicochemical treatments after immersion in acid solution, alkaline solution and solutions containing TiO2 and Ca2+, to obtain three different surfaces. These were characterized by electron microscopy and free energy estimates. The evaluation of the interaction with SBF was performed by measuring the dynamic contact angles after contacting the surfaces. RESULTS: The effects of SBF wettability were more significant on surfaces according to high energy estimates. A comparative analysis of the three types of surfaces showed that fluid spreading was greater in samples with greater polar components, indicating that the surface nature influences interactions in the early stages of osseointegration. CONCLUSION: The results indicate the influence of polar interactions in the dynamic wettability of the SBF. It is possible that these interactions can also influence cellular viability on surfaces. Based on these results, new experiments are being designed to improve the presented methodology as a tool for the evaluation of biomaterials without the need for in vivo experiments.