Reaction pathways in the solid state synthesis of multiferroic BiFeO 3

The obtaining of multiferroicBiFeO3 as a pure single-phase product is particularly complex since the formation of secondary phases seems to be unavoidable. The process by which these secondary impurities are formed is studied by analyzing the diffusion and solidstate reactivity of the Bi2O3–Fe2O3 system. Experimental evidence is reported which indicates that the progressive diffusion of Bi3+ ions into the Fe2O3 particles governs the solidstatesynthesis of the perovskite BiFeO3 phase. However a competition is established between the diffusion process which tends to complete the formation of BiFeO3, and the crystallization of stable Bi2Fe4O9 mullite crystals, which tend to block that formation reaction.

Aplicaciones estructurales de la guadua (Guadua angustifolia Kunth): Proyecto de estructura modular multifuncional en Colombia

La Guadua (Guadua angustifolia Kunth) es una de las especies forestales más características de Colombia. Perteneciente a la familia del bambú, ésta planta nativa de Colombia usada en la construcción permite una reducción de costos, garantizados, entre otras ventajas, por sus propiedades físico-mecánicas de flexibilidad, resistencia, dureza, efecto climatizado y sismo resistencia. En el proyecto se plantea inicialmente un estudio de la Guadua como material estructural. Se estudiará su anatomía, sus propiedades físicas y mecánicas así como la normativa existente que rige su uso en construcción y por último se darán algunos ejemplos en los que se puede comprobar su uso en la actualidad. Posteriormente se procederá al desarrollo del proyecto cuya finalidad es el diseño de una estructura modular multifuncional que se necesita para llevarla a cabo en un proyecto de cooperación localizado en Neiva (Colombia). El proyecto incluirá todos los documentos necesarios para un trabajo de estas características: memoria, pliego de condiciones técnicas, presupuesto, planos, así como el Estudio de Seguridad y Salud Laboral. Como solución a las necesidades planteadas en el proyecto de cooperación, se realizará el diseño de dos estructuras modulares cuya finalidad sea las de uso como oficina y como almacén. Se aplicará para su cálculo el CTE cómo normativa de construcción, pero apoyándose en algunos parámetros en la NSR-10, estableciendo una comparativa posterior entre ambas normativas. Debido a la importancia de la acción sísmica en Colombia se comprobará la resistencia sísmica del módulo diseñado según el “Manual de Construcción, evaluación y rehabilitación sismo resistente de viviendas de mampostería” creado por la Asociación Colombiana de Ingeniería Sísmica. Basándose en este manual se definirán los tipos de uniones más favorables sísmicamente de los módulos con el diseño de una cercha auxiliar necesaria según el tipo de unión. ABSTRACT PROJECT: Structural applications of the guadua (Guadua angustifolia Kunth). Project of modular multifunctional structure in Colombia Guadua (Guadua angustifolia Kunth) is one of the forest species more characteristics of Colombia. Pertaining to the family of the bamboo, this one native plant of Colombia used in the construction allows a reduction of costs, guaranteed, among others advantages, by its physic mechanical properties of flexibility, resistance, hardness, climatic effect and earthquake resistance. The project initially considers a study of the Guadua as structural material. We will study its anatomy, its physical and mechanical properties as well as the existing norm that governs its use in construction and finally some examples will be provided in which its actual use can be verified. Later on, we will proceed with the development of the project which purpose is the design of a multifunctional modular structure required to in a project of cooperation located in Neiva (Colombia). The project will include all the necessary documents for a work of these characteristics: memory, technical specifications, budget, drawings, as well as the Security and Labor Health Study. In order to satisfy the needs raised in the cooperation project, we will undertake the design of two modular structures with the purpose to be used as office and warehouse. The construction normative CTE will be applied for its calculation, but some parameters will be based in the NSR-10, establishing a comparison between both norms. Due to the importance of the seismic action in Colombia, the seismic resistance of the module will be verified according to the “Manual of Construction, evaluation and rehabilitation resistant earthquake of rubblework houses” created by the Colombian Association of Seismic Engineering,. We will also use this Manual to define the types of unions more favorable from seismic point of view, designing the required auxiliary segments according to the type of union.

SSONDE: Semantic Similarity On liNked Data Entities

Linked data offers a promising setting to encode, publish and share metadata of resources. As the matter of fact, it is already adopted by data producers such as European Environment Agency, US and some EU Governs, whose first ambition is to share (meta)data making their processes more effective and transparent. Such as an increasing interest and involvement of data providers surely represents a genuine witness of the web of data success, but in a longer perspective, frameworks supporting linked data consumers in their decision making processes will be a compelling need. In this respect, the talk is introducing SSONDE, a framework enabling in detailed comparison, ranking and selection of linked data resources through the analysis of their RDF ontology driven metadata. SSONDE implements an instance similarity especially designed to support in resource selection, namely the process stakeholders engage to choose a set of resources suitable for a given analysis purpose: (i) it deploys an asymmetric similarity assessment to emphasize information about gains and losses the stakeholders get adopting a resource in place of another; (ii) it relies on an explicit formalization of contexts to tailor the similarity assessment with respect to specific user-defined selection goals. The talk aims at providing an insight on SSONDE instance similarity and it will briefly describe some examples of SSONDE deployment in the context of linked data consumption.

Reaction pathways in the solid state synthesis of multiferroic BiFeO3

The obtaining of multiferroic BiFeO3 as a pure single-phase product is particularly complex since the formation of secondary phases seems to be unavoidable. The process by which these secondary impurities are formed is studied by analyzing the diffusion and solid state reactivity of the Bi2O3?Fe2O3 system. Experimental evidence is reported which indicates that the progressive diffusion of Bi3+ ions into the Fe2O3 particles governs the solid state synthesis of the perovskite BiFeO3 phase. However a competition is established between the diffusion process which tends to complete the formation of BiFeO3, and the crystallization of stable Bi2Fe4O9 mullite crystals, which tend to block that formation reaction.

Some framework ideas for software engineering education

It is difficult, if not impossible, to find something that is not changing in computer technology: circuits, architectures, languages, methods, fields of application ... The "central object" itself of this brand of engineering, software, represents such a diverse reality (many objects) that the fact that it has only one name gives rise to considerable confusion. This issue, among others, was taken up by Fox (1) and, at this point, I would like to underline that it is more of a pragmatic issue than an academic one. Thus, Software Engineering Education moves in an unstable, undefined'world. This axiom governs and limits the. validity of all educational proposals in the area of Software Engineering and, thereforer all the ideas presented in this paper.

Potential theory

In this chapter we will introduce the reader to the techniques of the Boundary Element Method applied to simple Laplacian problems. Most classical applications refer to electrostatic and magnetic fields, but the Laplacian operator also governs problems such as Saint-Venant torsion, irrotational flow, fluid flow through porous media and the added fluid mass in fluidstructure interaction problems. This short list, to which it would be possible to add many other physical problems governed by the same equation, is an indication of the importance of the numerical treatment of the Laplacian operator. Potential theory has pioneered the use of BEM since the papers of Jaswon and Hess. An interesting introduction to the topic is given by Cruse. In the last five years a renaissance of integral methods has been detected. This can be followed in the books by Jaswon and Symm and by Brebbia or Brebbia and Walker.In this chapter we shall maintain an elementary level and follow a classical scheme in order to make the content accessible to the reader who has just started to study the technique. The whole emphasis has been put on the socalled "direct" method because it is the one which appears to offer more advantages. In this section we recall the classical concepts of potential theory and establish the basic equations of the method. Later on we discuss the discretization philosophy, the implementation of different kinds of elements and the advantages of substructuring which is unavoidable when dealing with heterogeneous materials.

Micromechanical basis for shear strength of rock discontinuities

The theoretical basis for evaluating shear strength in rock joints is presented and used to derive an equation that governs the relationship between tangential and normal stress on the joint during situations of slippage between the joint faces. The dependent variables include geometric dilatancy, the instantaneous friction angle, and a parameter that considers joint surface roughness. The effect roughness is studied, and the aforementioned formula is used to analyse joints under different conditions. A mathematical expression is deduced that explains Barton's value for the joint roughness coefficient (JRC) according to the roughness geometry. In particular, when the Hoek and Brown failure criterion is used for a rock in the contact with the surface roughness plane, it is possible to determine the shear strength of the joint as a function of the relationship between the uniaxial compressive strength of the wall with the normal stress acting on the wall. Finally, theoretical results obtained for the geometry of a three-dimensional joint are compared with those of the Barton's formulation

Entre: de repente, sin fin, lo innombrable

Etiquetamos para reducir nuestra incertidumbre; clasificamos en base a términos opuestos para poder entender. El universo y el espacio construido en particular, se explican desde una arquitectónica dual: dentro – fuera, arriba – abajo, derecha – izquierda, día – noche, hombre – mujer. Lo que nos diferencia como grupo, raza o género, distinguiendo los modos de vivir, las ideologías y las teorías, es la relación que establecemos entre los términos opuestos: entre lo individual y lo colectivo, lo privado y lo público, la imaginación y la realidad, la identidad y la otredad, el orden y el caos, el deseo y la saciedad. El afán clasificatorio dicotómico de la realidad que la modernidad lleva a su extremo, hiere la vida: la mutua exclusión de los opuestos elimina la distancia, el espacio-tiempo entre ambos, que es donde la vida se sitúa. La concepción dual que rige nuestro conocimiento contamina también la relación entre los arquitectos y la sociedad, siendo la causa del ambiente tóxico que envuelve al habitante que ya no se identifica con los lugares que habita. Puede que sea oportuno pasar del pensamiento binario a una lógica de lo intersticial que abandone la dualidad para instalarse en el Entre; los arquitectos serían mediadores entre el poder y la vida, sintiendo el Entre como el transcurso de la vida y la potencia de interacción: los lugares ambiguos e intermedios, es donde sucede el encuentro entre los términos, entre arquitectos y habitantes, entre la imaginación y la realidad, entre tú y yo. ABSTRACT We label to reduce our uncertainty; we classify based on opposites to understand. The universe and all constructed space in particular, are explained from a dual architecture: inside - out, up - down, right - left, day - night, male - female, black - white. What differentiate us as a group, race, gender, distinguishing the lifestyles, ideologies and theories, is how we draw the relation between the opposites: between the individual and the collective, private and public, imagination and reality , identity and otherness, order and disorder, desire and satiety. The dichotomous classification of reality that modernity carried to its extreme, hurts life: the mutual exclusion of opposites eliminates the distance, the space-time between the two, which is where life is located. The dual conception that governs our knowledge has also contaminated the relationship between architect and society, being the cause of the toxic environment that surrounds the inhabitant who no longer identify himself with the places he inhabit. It may be appropriate pass from binary thinking to a logic of the interstitial which abandon duality to settle in Between; architect then will be the mediator between authorities and life, sensing the Between as a flowing of life a potency of interaction rather than a separation between the extremes; ambiguous, in –between, places are where the encounter between opposites happens, between the architect and the inhabitant, between imagination and reality, between you and me.

La construcción del espacio litoral : el caso de la costa de Cartagena

El litoral ha constituido a lo largo de la historia una importante fuente de recursos económicos además de un punto estratégico para la defensa del territorio. El comercio, la pesca o la industria de la sal han propiciado la formación de poblaciones y ciudades al borde del mar. La costa funcionó como un ámbito defensivo, peligroso e insalubre durante siglos donde el mar modulaba el frente costero sin grandes interacciones con un entorno prácticamente deshabitado. A mediados del siglo XIX, las innovaciones técnicas y la pacificación definitiva del Mediterráneo permiten poner en valor sus características naturales y de oportunidad. Sin perder del todo su valor defensivo, el litoral resurge como recurso productivo y como lugar de ocio y disfrute de la población. El valor estratégico de la costa quedará también reflejado en el marco normativo. El derecho civil moderno recuperará el concepto de Dominio público Marítimo Terrestre a través de la Ley de Aguas de 1866 que regulará también los usos y las limitaciones en la propiedad privada litoral. Desde ese momento, las transformaciones económicas, sociales, jurídicas y ambientales van a provocar un cambio significativo en la relación entre la sociedad y la costa que dará paso a la construcción del espacio litoral que hemos heredado. Desde la triple perspectiva del litoral como sistema físico ambiental, sistema económico, productivo y cultural, y sistema administrativo y legal, el principal objetivo de la tesis será reconstruir el proceso de transformación del litoral consecuencia de la evolución en la forma de producción del espacio, de área defensiva a recurso productivo, y desde mediados del siglo XIX hasta principios del siglo XXI. Sin embargo, la construcción del litoral no ha sido homogénea ni constante a lo largo del tiempo. Ha estado sujeta a los distintos vaivenes económicos y sociales pero también a los cambios en el modelo territorial definido por el marco legal vigente, así como a los reajustes del propio sistema físico. Como instrumento sociopolítico, el marco legal regula las presiones del sistema económico sobre el medio, apostando por una visión frente a otra, y posibilitando el grado de transformación final. Así, el conocimiento sobre el territorio en el que se interviene y la definición del modelo de litoral por parte de los poderes públicos irán configurando el espacio físico, económico y social desarrollado en la costa. Para la reconstrucción del proceso de construcción del litoral, la tesis define cuatro fases diferentes y aplica las hipótesis y la metodología a la costa de Cartagena. Se presenta gráficamente la evolución en los tres sistemas en cada una de las fases, y se recompone el relato histórico a través de los hitos más relevantes para el proceso. En cada una de las fases, el nuevo modelo intentará dar respuesta a todo aquello que no funcionó o se quedó a medias en el periodo anterior. Las crisis económicas provocan la ralentización de la actividad productiva y, consecuentemente, de las transformaciones en el territorio. Servirán para establecer, en principio, un cambio de paradigma en la lectura y gestión del litoral que acabará traduciéndose en un nuevo texto legislativo en materia de costas (1969, 1988 y 2013). La reforma de la normativa responde a una nueva forma de entender, ordenar, gestionar e intervenir en el territorio, donde se modulan las pautas pero también la intensidad en la intervención. Pero nace condicionada por el litoral heredado: los derechos generados durante la vigencia del marco legal anterior; el modelo económico y sus presiones y expectativas sobre el litoral; y el medio físico en el que se acumulan los procesos de degradación no resueltos y los nuevos riesgos. Así, las conclusiones de la tesis ponen de manifiesto la necesidad de una visión compleja e integral sobre el litoral, en la que el urbanismo y la ordenación del territorio serán fundamentales para afrontar los nuevos retos en su construcción a futuro. ABSTRACT The littoral has been throughout history an important source of economic resources and a strategic point for the defense of territory. Trade, fishing or salt industry have led to the formation of towns and cities on the edge of the sea. The coast served as a defensive, dangerous and unhealthy place where the sea modulated for centuries the waterfront without major interactions with a virtually uninhabited environment. In the mid-nineteenth century, technical innovations and the final pacification of the Mediterranean allowed to value its natural features and opportunities. Without entirely losing its defensive value, the coast emerges as a productive resource and as a place of leisure and enjoyment of people. The strategic value of the coast will also be reflected in the legal framework. The concept of maritime-terrestrial public domain will be recovered by the modern civil law and the law of waters of 1866 governs the uses and limitations of private ownership of the coast. Since then, the economic, social, legal and environmental changes will to cause a significant change in the relationship between society and the coast that will give way to the construction of littoral space inherited. From the triple perspective of the littoral as a physical environmental system, an economic, productive and cultural system, and an administrative and legal system, the main objective of the thesis is to rebuild its process of transformation, as a result of the evolution in the way that space is produced, from defensive zone to productive resource, and from the middle of the nineteenth century until the beginning of the twenty-first century. However, the construction of the coast has not been uniform nor constant over time. It has been subject to different economic and social fluctuations and also to changes in the territorial model defined by the legal framework in force, as well as to readjustments of the physical system itself. As socio-political instrument, the legal framework regulates the pressure of the economic system on the environment, it bets on a vision over another and facilitates the final degree of transformation. Thus, the knowledge on the territory that is being intervened and the definition of the model of shoreline by public authorities will configure the physical, economic and social space developed on the coast. For the reconstruction of the littoral building process, the thesis defines four different phases and applies the hypothesis and methodology to the coast of Cartagena. It introduces graphically the evolution of the three systems in each of the phases and it recomposes the historical account through of the most important milestones for the process. In each phase, the new model will attempt to answer everything that did not work or was half in the previous period. Economic crises cause a slowdown in productive activity and, consequently, in the changes of territory. They serve to establish, in principle, a paradigm shift for reading and managing the littoral, eventually resulting in new legal texts on coasts matter (1969, 1988 and 2013). The reform of legislation responds to a new way for understanding, arranging, managing and intervening on the territory, where the guidelines and also the intervention intensity are modulated. But it is born conditioned by the inherited coast: the rights generated under previous legal framework; the economic model and its pressures and expectations on the littoral; and the physical environment which accumulates degradation processes unresolved and new risks. Thus, the thesis conclusions highlight the need for a complex and comprehensive view on the littoral, where urban planning and land-use planning will be key to meet the future challenges in its construction.

En un intervalo del límite : una nueva lectura del movimiento moderno desde la realidad virtual de lo ambiguo

Esta tesis doctoral busca estudiar el espacio desde la premisa de que el espacio es, fundamentalmente, lo intermedio. El entre, lo que hay entre las cosas mismas y que les da, precisamente, su definición como cosas en un constante proceso de delimitación. Este entre, lo que hay entre las cosas, no es sin embargo un resto que queda, sino bien al contrario el principio activo que hace que las cosas, desde lo que hay alrededor, se configuren en su ser sensible y puedan percibirse. El entre, lo intermedio, no es así una línea, un corte puro, sino un intervalo, un espacio en sí mismo, un ámbito en que se desarrolla un proceso. Es por tanto un espacio de formación, en el que las cosas aún no son y ya están siendo, un intervalo ambiguo pleno de virtualidades, unas desplegadas otras que permanecen en espera, siempre en movimiento. Es la hipótesis principal de esta tesis que en el llamado Movimiento Moderno en la arquitectura del siglo veinte esta condición ambigua del límite se pone de manifiesto como la raíz articuladora de los cambios espaciales y formales que definen su ser y su estética. Para desarrollar esta hipótesis, se parte de una doble premisa: por una parte, como un entre que es en sí misma, la arquitectura se encuentra delimitada por las disciplinas artísticas contemporáneas, especialmente pintura y escultura y a la vez las delimita. Por tanto, se explorarán una serie de momentos claves en las vanguardias de comienzos del siglo veinte en paralelo a una serie de arquitecturas contemporáneas para estudiar esta doble dialéctica entre límites. Por otra parte, y entendiendo que lo propio de la arquitectura es el espacio, se estudiarán en profundidad las concepciones espaciales que en la física y la estética del finales del siglo diecinueve y principios del veinte se desarrollan, para comprender cómo estas nuevas concepciones, centradas en las ideas de relatividad y de espacio-tiempo como magnitudes interlimitadas, determinan a su vez las concepciones espaciales que se desarrollan simultáneamente en las artes plásticas y en la arquitectura. La tríada espacio/tiempo/luz regirá este recorrido, en un doble camino: a través de la física y la teoría del arte, y a través de la arquitectura y las artes plásticas. Se trata por tanto de incardinar la arquitectura en su contexto artístico y científico, y comprobar cómo la comprensión del espacio como un entre, como un intervalo del límite y no como un absoluto trascendente, se generaliza en dichos ámbitos en una exploración paralela que condiciona los resultados en todos ellos y que define, por tanto, la arquitectura de la modernidad como una arquitectura del entre. El encuadre enmarca como motivo principal el Movimiento Moderno a través de la figura de Le Corbusier. Se muestra el modo en que los recursos y mecanismos empleados provocan el encuentro del espacio indecible a través de la polifonía de las artes. Desde el espaciamiento del límite, su borradura, su ambigüedad, producidos mediante el entrelazamiento de los recursos artísticos, la continuidad de lo lineal, el encabalgamiento, el maridaje, la ambivalencia, la relatividad del color y la luz, el intersticio. Ello se analiza a través de la obra de le Corbusier, destacando la dialéctica entre Le Cheminée y Nature morte aux nombreux objets; Ma maison y el pabellón del Zurichhorn; Unité y L’espace indicible. Aclaradas las premisas fundamentales y las consecuencias inmediatas para la arquitectura del Movimiento Moderno, la cuarta parte y final de la tesis, mucho más breve que las anteriores, expone una primera aproximación a las consecuencias que todo esto ha tenido en un momento liminal también: el comienzo de la postmodernidad. En realidad se trata de una apertura a lo que ha de venir, pues establecida la operatividad de la hipóteisi inicial, se trata aquí solamente de abrir el camino a una interpretación que en realidad escapa del alcance de esta tesis. Así se deja apuntado cómo, a través de la obra de tres arquitectos, teóricos y artistas trabajando en los años setenta y comienzos de los ochenta del pasado siglo, una comprensión más clara de la revolución moderna precipitada por esta nueva comprensión del espacio como límite-entre queda patente. Chamberworks de Daniel Libeskind, Wexner Center for the Visual Arts de Peter Eisenman y Manhattan Transcripts de Bernard Tschumi servirían de privilegiados ejemplo. Como queda patente que las consecuencias de todo ello aún las estamos sintiendo en nuestra contemporaneidad. ABSTRACT This thesis aims to study the space from the premise that space is mainly the in-between. The in-between, what is in-between the things themselves and gives them precisely its definition as things in a constant process of delimitation. This in-between, what is in between things, is not a remnant that remains, but on the contrary it is the active ingredient that produces things from what is around, that configures them in a sensitive to be perceived. The in-between, the intermediate, is not a line, a pure cut, but an interval, a space in itself, an area in which a process is developed. It is therefore a space in development, where things are still not and already been, delimiting an ambiguous range full of potentialities, some present and others that remain only as virtualities. It is the main hypothesis of this thesis that in the so-called Modern Movement in the architecture of the twentieth century this ambiguous boundary condition is revealed as the result of the articulatation of spatial and formal changes. To develop this hypothesis, I posit this double premise: On the one hand, as an in-between which is in itself, architecture is defined by contemporary artistic disciplines, especially painting and sculpture and simultaneously defines them. Therefore, a number of key moments in the avantgarde of the early twentieth century in parallel with a number of contemporary architecture to study this double dialectic between boundaries will be explored. On the other hand, and considering that the “essence” of the architecture is space, I will study in depth the spatial concepts in physics and aesthetics of late-nineteenth century and early twentieth century to understand how these new concepts centered on the ideas of relativity and space-time as interlimited magnitudes in turn determine the spatial conceptions that take place simultaneously in the visual arts and architecture. The space / time / light triad governs this journey in a double way: through physics and theory of art, and through architecture and the arts. The aim is to anchor architecture in its artistic and scientific context, and to see how the understanding of space as an in-between interval and not as an absolute transcendent determines the results in all of them, defining, therefore, the architecture of modernity as an architecture of the in-between. The frame of the study is centered in the modern movement through the figure of Le Corbusier and how rthe different resources and mechanisms employed provoke an unspeakable space of encounter through the polyphony of the arts. From the spacing of the limit, its erasure, its ambiguity, produced by the intertwining of artistic resources to continuity of the linear, the marriage, the ambivalence, the relativity of color and light, the gap. All this is analyzed through the work of Le Corbusier, highlighting the dialectic between Le Cheminée and Nature morte aux nombreux objets, Ma maison and Zürichhorn pavilion, Unité and L’espace indicible. Once the basic premises and immediate consequences for the architecture of the Modern Movement are set, the fourth and final part of the thesis aims to explore the impact that all this has had in another liminal time: the beginning of postmodernism. Of course, this part is shorter, in fact it is more a prospective survey and an opening of possibilities than a truly articulated body of theory, since the conceptual core of this thesis has been developed in the central part. Hence, through the work of three architects, theorists and artists working in the seventies and early eighties of last century, a clearer understanding of the modern revolution precipitated by this new understanding of space as a boundary- between is proposed. Chamberworks by Daniel Libeskind, Wexner Center for the Visual Arts by Peter Eisenman and Manhattan Transcripts by Bernard Tschumi will exemplify this approach. The consequences of all this are still haunting our contemporaneity.

The phosphorylation state of the FIGQY tyrosine of neurofascin determines ankyrin-binding activity and patterns of cell segregation

Cell–cell recognition and patterning of cell contacts have a critical role in mediating reversible assembly of a variety of transcellular complexes in the nervous system. This study provides evidence for regulation of cell interactions through modulation of ankyrin binding to neurofascin, a member of the L1CAM family of nervous system cell adhesion molecules. The phosphorylation state of the conserved FIGQY tyrosine in the cytoplasmic domain of neurofascin regulates ankyrin binding and governs neurofascin-dependent cell aggregation as well as cell sorting when neurofascin is expressed in neuroblastoma cells. These findings suggest a general mechanism for the patterning of cell contact based on external signals that regulate tyrosine phosphorylation of L1CAM members and modulate their binding to ankyrin.

A Late Mitotic Regulatory Network Controlling Cyclin Destruction in Saccharomyces cerevisiae

Exit from mitosis requires the inactivation of mitotic cyclin-dependent kinase–cyclin complexes, primarily by ubiquitin-dependent cyclin proteolysis. Cyclin destruction is regulated by a ubiquitin ligase known as the anaphase-promoting complex (APC). In the budding yeast Saccharomyces cerevisiae, members of a large class of late mitotic mutants, including cdc15, cdc5, cdc14, dbf2, and tem1, arrest in anaphase with a phenotype similar to that of cells expressing nondegradable forms of mitotic cyclins. We addressed the possibility that the products of these genes are components of a regulatory network that governs cyclin proteolysis. We identified a complex array of genetic interactions among these mutants and found that the growth defect in most of the mutants is suppressed by overexpression of SPO12, YAK1, and SIC1 and is exacerbated by overproduction of the mitotic cyclin Clb2. When arrested in late mitosis, the mutants exhibit a defect in cyclin-specific APC activity that is accompanied by high Clb2 levels and low levels of the anaphase inhibitor Pds1. Mutant cells arrested in G1 contain normal APC activity. We conclude that Cdc15, Cdc5, Cdc14, Dbf2, and Tem1 cooperate in the activation of the APC in late mitosis but are not required for maintenance of that activity in G1.

An alternative pathway to β-carotene formation in plant chromoplasts discovered by map-based cloning of Beta and old-gold color mutations in tomato

Carotenoid pigments in plants fulfill indispensable functions in photosynthesis. Carotenoids that accumulate as secondary metabolites in chromoplasts provide distinct coloration to flowers and fruits. In this work we investigated the genetic mechanisms that regulate accumulation of carotenoids as secondary metabolites during ripening of tomato fruits. We analyzed two mutations that affect fruit pigmentation in tomato (Lycopersicon esculentum): Beta (B), a single dominant gene that increases β-carotene in the fruit, and old-gold (og), a recessive mutation that abolishes β-carotene and increases lycopene. Using a map-based cloning approach we cloned the genes B and og. Molecular analysis revealed that B encodes a novel type of lycopene β-cyclase, an enzyme that converts lycopene to β-carotene. The amino acid sequence of B is similar to capsanthin-capsorubin synthase, an enzyme that produces red xanthophylls in fruits of pepper (Capsicum annum). Our results prove that β-carotene is synthesized de novo during tomato fruit development by the B lycopene cyclase. In wild-type tomatoes B is expressed at low levels during the breaker stage of ripening, whereas in the Beta mutant its transcription is dramatically increased. Null mutations in the gene B are responsible for the phenotype in og, indicating that og is an allele of B. These results confirm that developmentally regulated transcription is the major mechanism that governs lycopene accumulation in ripening fruits. The cloned B genes can be used in various genetic manipulations toward altering pigmentation and enhancing nutritional value of plant foods.

Dynamic modeling of gene expression data

We describe the time evolution of gene expression levels by using a time translational matrix to predict future expression levels of genes based on their expression levels at some initial time. We deduce the time translational matrix for previously published DNA microarray gene expression data sets by modeling them within a linear framework by using the characteristic modes obtained by singular value decomposition. The resulting time translation matrix provides a measure of the relationships among the modes and governs their time evolution. We show that a truncated matrix linking just a few modes is a good approximation of the full time translation matrix. This finding suggests that the number of essential connections among the genes is small.

QscR, a modulator of quorum-sensing signal synthesis and virulence in Pseudomonas aeruginosa

The opportunistic pathogenic bacterium Pseudomonas aeruginosa uses quorum-sensing signaling systems as global regulators of virulence genes. There are two quorum-sensing signal receptor and signal generator pairs, LasR–LasI and RhlR–RhlI. The recently completed P. aeruginosa genome-sequencing project revealed a gene coding for a homolog of the signal receptors, LasR and RhlR. Here we describe a role for this gene, which we call qscR. The qscR gene product governs the timing of quorum-sensing-controlled gene expression and it dampens virulence in an insect model. We present evidence that suggests the primary role of QscR is repression of lasI. A qscR mutant produces the LasI-generated signal prematurely, and this results in premature transcription of a number of quorum-sensing-regulated genes. When fed to Drosophila melanogaster, the qscR mutant kills the animals more rapidly than the parental P. aeruginosa. The repression of lasI by QscR could serve to ensure that quorum-sensing-controlled genes are not activated in environments where they are not useful.