Copy-choice recombination mediated by DNA polymerase III holoenzyme from Escherichia coli.

Formation of deletions by recombination between short direct repeats is thought to involve either a break-join or a copy-choice process. The key step of the latter is slippage of the replication machinery between the repeats. We report that the main replicase of Escherichia coli, DNA polymerase III holoenzyme, slips between two direct repeats of 27 bp that flank an inverted repeat of approximately equal 300bp. Slippage was detected in vitro, on a single-stranded DNA template, in a primer extension assay. It requires the presence of a short (8 bp) G+C-rich sequence at the base of a hairpin that can form by annealing of the inverted repeats. It is stimulated by (i) high salt concentration, which might stabilize the hairpin, and (ii) two proteins that ensure the processivity of the DNA polymerase III holoenzyme: the single-stranded DNA binding protein and the beta subunit of the polymerase. Slippage is rather efficient under optimal reaction conditions because it can take place on >50% of template molecules. This observation supports the copy-choice model for recombination between short direct repeats.

Delays in nuclear reactor licensing and construction : the possibilities for reform.

"February 1979."

Latest contemporary portraits,

"Second printing, April, 1928."

Latest contemporary portraits,

David Graham Phillips: the greatest American novelist.--Eleonora Duse.--Henri Barbusse.--Lord Bryce.--George Russell (A.E.)--Mrs. Humphry Ward.--Eugene V. Debs.--Charles Schwab: the master of Bethlehem.--Prince Peter Kropotkin.--Paul Bourget.--Thomas Hardy.--Ehrlich of "606".--Louis Sullivan: a great architect.--Eugene Fromentin: the painter writer.--Sir Herbert Tree.--Flaubert and his letters.--Hatred in art: Leon Bloy.--Three generations of Morgans.--The brothers de Goncourt and realism.--Lord Hartington.--Ambassador Bernstorff.--A talk with A.E. Housman.--Paul Deschanel.--Frederic Harrison: the last of the Victorians.--Annie Besant: rebel and reformer.--John Churton Collins.--Hyndman, the communist.--Horace Traubel.--Joseph Caillaux.

Photographic dosimetry of X- and gamma rays.

Bibliography: p. 28.

Daily studies for the piano /

Mode of access: Internet.

A truck and bus tire use pattern study. Final report.

National Bureau of Standards, Tire Systems Section, Washington, D.C.

Das Königreich Böhmen, statistisch-topographisch dargestellt /

Vols. 9-16 published by F. Ehrlich, Prag.

Metaphysik als rationale Ontologie.

Mode of access: Internet.

A collection of theses, etc., on philology.

1. Bradke, P. Über die arische Alterthumswissenschaft und die Eigenart unseres Sprachstammes.--2. Wijk, N. Der Nominale Gevetiv Singular Im Indogermanischen.--3. Lommel, H. Studien über Indogermanische Femininbildungen.--4. Ehrlich, H. Zur indogermanischen Sprachgeschichte.--5. Heymann, W. Das L der indogermanischen Sprachen gehört der indogermanischen Grundsprache an.--6. Vesely, A. Wie ist die Schrift entstanden?--7. Michaelis, G. Über die Anordnung der Vokale.--8. Meillet, A. De indo-evropaea radice *Men- "Mente Agitare".--9. Velics, A. Ueber die Einheit Der Sprachen.--10. Egger, J. Studien zur Geschichte des indogermanischen Consonantismus.--11. Torbiörnsson, T. Die vergleichende Sprachwissenschaft.--12. Osthoff, H. Vom Suppletivwesen der indogermanischen Sprachen.--13. Schmidt, J. Die Urheimath Der Indogermanen und das Europäische Zahlsystem.--14. Zürich. Universität. Philosophische facultät. Philologische Abhandlungen Heinrich Schweizer-Sidler gewidmet.

Collected studies on immunity /

Translation of his Gesammelte Arbeiten zur Immunitätsforschung.

Die lieben der Sibylle Burg; roman ...

Mode of access: Internet.

Ihre galanten abenteuer ...

Mode of access: Internet.

Sonette an einen Knaben /

"Mit Zeichnungen von Georg Ehrlich in Wien"--T.p. verso.

Frau Mimis vergangenheit; kriminalroman aus schieberkreisen ...

Mode of access: Internet.