Atividade in vivo de parasporina extraída de B. thuringiensis sobre o câncer colorretal murino (CT‐26)

Bacillus thuringiensis (Bt), is an environmental Gram-positive spore-forming bacterium that produces crystalline parasporal protein (Cry) during sporulation. The inclusions often exhibit strong and specific insecticidal activity, making Bt an agent for agricultural controlling insects pest, mites, protozoa and nematodes. Recent studies reported that some of these Crys do not show cytotoxicity against insects but they are capable to kill some human and animal cancer cells. These proteins were denominated parasporins (PS). However, antitumor activity of Bt parasporin on the development of murine colorectal cancer (CT-26), are not well studies and these are no reports on the in vivo effect of these proteins. Thus, the present study evaluated the in vitro and in vivo anti-tumoral activity of Bt parasporin against the murine colorectal cancer line CT-26. Therefore, Balb/c mice were s.c. inoculated with CT-26 cells and weekly treated with parasporin (i.p.) pre-activated by enzymatic digestion with trypsin or proteinase K. Our results have shown, for the first time, that despite the anti-tumor activity in vitro, parasporin crystals couldn’t combat tumor growth in vivo. Instead, this protein was highly toxic, affecting the liver and spleen, with possible effect on other organs, decreasing the survival of treated animals. The results indicate the need for studies to better detoxification or manipulation of parasporin for therapeutic use and new studies for analysis of toxicological effects of repetitive exposure of farmers to this toxin

Avaliação da qualidade da atenção ao parto nos colegiados de gestão regional Cuesta Botucatu e Vale do Jurumirim

The humanization of childbirth implies the understanding of this event as an important experience, and requires the redefinition of human relationships from the review of the assistance project, the understanding of pregnant women condition and human rights. To evaluate child birth assistance using a score that was developed by Botucatu Cuesta Regional Management and Jurumirim Valley Collegiates. This is an epidemiological, descriptive study that is inserted in the field of evaluation of services, programs or health projects. The data has been obtained by direct observation of deliveries, emphasizing the humane care. Results: Approximately one third of the women gave birth in a tertiary hospital (34.1%) and it was their first babies (33%).24.8% of the women received prenatal care in high-risk services. 67.1% of the births were normal, and 84.7% had no companions in the delivery room. In 47.1% of the cases the delivery was performed by obstetrician who used anesthesia in 44.7% and episiotomy in 48.2% of the deliveries. More than half of the newborns were attended by the pediatrician in the delivery room and had a delivery graph completed. Although the present study shows that 67.1% of the births were normal, caesarean rate can be considered excessive, as the WHO points out that c-sections above 15% are unlikely to be justifiable. It is important to emphasize that the Ministry of Health has to have a commitment with all women to promote safe motherhood, even in cases when the pregnancy involves a risk for both the mother and the fetus. It is noteworthy that the created score allowed us to assess variables related to the humanization of childbirth and only average and quite similar situations among the three services were evidenced. We hope that with this study, managers and professionals that work in this area can be subsidized in order to offer effective humane assistance and quality service in the delivery

Germinação, desenvolvimento e colheita da semente na mulher-terra: orientação de gestantes do SUS de Rio Claro sobre gestão e parto

Ao viver uma gestação, a mulher passa por mudanças biológicas, somáticas, psicológicas e sociais. É de fundamental importância que as gestantes recebam apoio, sejam orientadas sobre tais mudanças e também sobre o parto, momento comumente temido por elas. A assistência pré-natal é oferecida em nível nacional pelo Ministério da Saúde, que propõem a realização de ações educativas e a criação de grupos de apoio, que visam complementar o atendimento realizado nas consultas médicas. Porém, apesar de serem preconizadas, tais ações ainda são insuficientes ou insatisfatórias. O presente estudo visa contribuir com a área de Educação em Saúde buscando, a partir do conhecimento da realidade de gestantes, realizar uma ação de orientação, com a intenção de complementar o pré-natal realizado em Unidades de Saúde da Família (USFs). Para tanto, o objetivo geral da pesquisa foi investigar aspectos do conhecimento e dos sentimentos de gestantes usuárias do SUS de Rio Claro a respeito da gestação e parto, orientando-as sobre tais assuntos. A pesquisa, com abordagem Qualitativa, objetivo Descritivo e que usou como procedimento técnico a Pesquisa-ação, ocorreu no município de Rio Claro e teve como sujeitos 15 gestantes. A coleta de dados se deu através de entrevistas semi-estruturadas, que foram gravadas e de um diário de campo. Foi elaborado um material contendo 16 imagens coloridas, que foram utilizadas nas orientações individuais com o intuito de esclarecer os processos da gestação e parto. Os dados foram analisados através da técnica de Análise de Conteúdo. A maioria das entrevistadas afirmou que não planejava engravidar e, ainda assim, mais da metade delas não fazia uso de nenhum método contraceptivo, mostrando que não houve um planejamento familiar efetivo. As fontes de informação mais citadas foram as pessoas mais velhas e/ou experientes... (Resumo completo, clicar acesso eletrônico abaixo)

Alterações metabólicas e morfométricas induzidas por hipóxia em Gallus gallus

Pós-graduação em Zootecnia - FCAV

Condições de saúde bucal das gestantes atendidas na atenção primária do município de Botucatu-SP e seu conhecimento sobre a inportãncia da saúde bucal na gestação

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Frequência e perfil de gestantes com toxoplasmose em uma unidade de saúde de Matão - SP

Congenital toxoplasmosis is a serious public health case, for it causes irreversible damage to the embryo/fetus, which may cause its death. The identification and the care to pregnant women with suspect acute toxoplasmosis (IgM+) is performed in prenatal monitoring. This study aimed to measure the incidence and know the profile of positive pregnant women for toxoplasmosis in a Basic Health Unit (BHU) in Matão - SP, between the years 2011 to 2013. This is a retrospective descriptive study, from the medical records of pregnant women attended. The project was approved by the Municipal Departament of Health and BHU was chosen along the Municipal Epidemiological Surveillance. From 2011 to 2013, 189 women began prenatal care in the unit, an annual median of 71(±26.91), of which 17 (8.99%) were positive for the serological test indicative of acute phase (IgM+). The distribution over the trial period was: four cases in 2011, twelve cases in 2012 and one in 2013. Pregnant women IgM positive for toxoplasmosis attend by BHU were: age 24(±5.47) years; color: equally distributed among white, black and brown; as the number of pregnancies: multiparous (2±0.97), most of them with a cesarean delivery as obstetric history and possessed no other risk factors associated with pregnancy (94.12%); gave entrance at BHU with 13.65(±7.35) weeks of gestation and had a median of 5(±2.36) consultation on their prenatal care. The examination for toxoplasmosis was requested as recommended by the Health Ministry (HM) and the medication prescribed was Roxamicina® - spiramycin, as soon as the test results (IgM+) arrived. Of the 17 pregnant women, only 10 completed the pre-natal at BHU - attendance at the postpartum consult (58.8%). Of these, the deliveries were vaginal (55.55%), made preterm with 36.5 weeks of gestation at the Municipal Hospital. The babies were born alive (100%) with the weight of 2.68(±0.77) Kg and required special care hospital scope. From the ...

Itinerário terapêutico de mães de crianças que foram a óbito no período neonatal em município do interior paulista

Pós-graduação em Enfermagem - FMB

Atividade física na gestação: aplicabilidade de um questionário específico para gestantes (PPAQ) e sua associação com Diabete Melito Gestacional (DMG), sobrepeso/obesidade

Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB

Qualidade de vida e satisfação das gestantes portadoras de diabete em uso de insulina em hospital universitário do interior de São Paulo

Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB

Avaliação eletrocardiográfica em fêmeas prenhes, fetos e neonatos equinos da raça Paint Horse

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Álcool, fumo e problemas periodontais em gestantes atendidas no sistema público de saúde

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Saúde coletiva: promoção de saúde bucal na gravidez

Objective: The aim of this study was to analyze the perception and attitudes about oral health of pregnant women recorded on SIS-Prenatal from Bilac city, São Paulo State. Method: the data collection was through interviews with pregnant women using a semi-structured questionnaire. Pregnant women that were not present on attendance with gynecologist and those whose children were born were excluded of sample. Result: Among the  20 interviewed pregnant women, 80% didn’t received any type of information about oral health during pregnancy, 45% of total didn’t know how to maintain baby’s oral health,  85% didn’t know the mean of carie disease. The majority of pregnant women (95%) interested to make part of projects about promotion of oral health. Conclusion: the future mothers showed little knowledge in relation to preventive proceedings in dentistry and about diseases that could involve their and baby’s oral health. It’s necessary to perform programs about oral health, pointing the cares for mother-baby

Doença periodontal, diabete mellitus e hipertensão em gestantes usuárias do Sistema Único de Saúde (SUS)

Purpose: To evaluate the association among periodontal disease, diabetes mellitus and arterial hypertension in pregnant women users of the Brazilian Health System (SUS). Methods: The sample consisted of 86 women, with pregnant age between the 4th and 7 th month, submitted to pre-natal attendance in Health Basic Unities (HBUs). Periodontal condition was evaluated by 2 calibrated researchers (Kappa = 0.91) through the Community Periodontal Index (CPI). A sphygmomanometer was used to check the arterial pressure, what was accomplished by the professionals from the HBUs; glycemia test was accomplished through a blood sample collected from the middle finger with a disposable lancet adapted to a lance device (Accu-Chek Softclix Pro; Roche, USA), and for checking the glycemia level, a glycemia monitor was used (Accu-Chek Advantage II, Roche, USA). Data were written on a numbered register form, avoiding patient identification. After, the same data were statistically analyzed through the Fisher Exact Test, in order to evaluate the existence of association between the variables blood glucose and periodontal disease. In this test, a significance level of 0,05 was adopted. Results – Most of the pregnant woman presented periodontal health (58,1%); 41,9% of the research subjects showed at least one sign of periodontal disease, with 31,4% presenting gingivitis and 10,5% periodontitis. Hyperglycemia was detected in 51,2% and it was not found a value of arterial pressure higher than what is considered to be normal. Conclusion: it was not verified a significant association among the presence of periodontal disease, glycemia alteration and arterial hypertension in pregnant women who comprised this research.

Binômio mãe-filho: estudo epidemiológico e de base populacional desenvolvido em maternidades de baixo e alto risco

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento infantil: associação com estresse, ansiedade e depressão materna, da gestação ao primeiro ano de vida

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)