Localization patterns of steroid and luteinizing hormone receptors in the corpus luteum of Nelore (Bos taurus indicus) cows throughout the estrous cycle

The aim of the present study was to detect progesterone receptors (A and B isoforms), α and β estrogen receptors, luteinizing hormone receptors and aromatase cytochrome P450 enzymes in the corpus luteum of Nelore (Bos taurus indicus) cows using immunohistochemistry. The estrous cycles of 16 Nelore cows were synchronized, and luteal samples were collected via an incision into the vaginal vault. Samples were collected during specific days of the estrous cycle (days 6, 10, 15 and 18) and 24. h after circulating progesterone dropped, after luteolysis had occurred. After each biopsy was taken, all animals were resynchronized so that each biopsy was performed during a different estrous cycle. Our results showed that the concentration of studied proteins vary throughout the bovine estrous cycle. The highest concentration of α and β estrogen receptors and the highest concentration of plasma progesterone were both observed on days 10 and 15 of the estrous cycle. The highest concentration of progesterone receptors was observed on days 6 and 10 of the estrous cycle, and the most intense immunostaining for cytochrome P450 aromatase enzymes was observed on day 10 of the estrous cycle. The highest score of cells with plasma membrane immunostaining for LH receptors was observed on day 15 of the estrous cycle. In conclusion, this study demonstrates the varying concentrations of specific proteins within the corpus luteum of Nelore cows during the estrous cycle. This finding suggests that these receptors and enzymes, and their interactions, are important in regulating luteal viability. © 2013 Elsevier B.V.

Corpus Luteum Development and Function after Supplementation of Long-Acting Progesterone During the Early Luteal Phase in Beef Cattle

Contents: Strategic supplementation of P4 may be used to increase conception rates in cattle, but timing of supplementation in relation to ovulation, mass of supplementary P4 and formulation of the P4-containing supplement has not been determined for beef cattle. Effects of supplementation of long-acting progesterone (P4) on Days 2 or 3 post-ovulation on development, function and regression of corpus luteum (CL) were studied in beef cattle. Cows were synchronized with an oestradiol/P4-based protocol and treated with 150 or 300 mg of long-acting P4 on Day 2 or 3 post-ovulation (6-7 cows/group). Colour-doppler ultrasound scanning and blood sample collection were performed from Day 2-21.5. Plasma P4 concentrations were greater (p < 0.05) from Day 2.5-5.5 in the Day 2-treated groups and from Day 3.5-5.5 in the Day 3-treated cows than in the control group. CL area and blood flow during Day 2-8.5 did not differ (p > 0.05) among groups, suggesting no effect of P4 treatment on luteal development. The frequency of cows that began luteolysis before Day 15 was greater (p < 0.04) in cows treated with 300 mg than in the controls, but there were no differences between non-treated and 150 mg-treated cows. The interval from pre-treatment ovulation to functional and structural luteolysis was shorter (p < 0.01) in the combined P4-treated groups than in the control cows. In conclusion, was showed for the first time that long-acting P4 supplementation on Day 2 or 3 post-ovulation increases P4 concentrations for ≥3 day, has no effect on luteal development, but anticipates the beginning of luteolysis in beef cattle. © 2013 Blackwell Verlag GmbH.

Uma análise da tradução de marcadores culturais em Sergeant Getulio e The Lizard's smile, à luz da linguística de corpus

Pós-graduação em Estudos Linguísticos - IBILCE

Tipologia e uso da voz média em Apolodoro: estudo semântico baseado em corpus

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Uma investigação de traduções de textos da área médica sob a luz dos estudos da tradução baseados em corpus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A tradução e os prazeres vivos de descobrir o mundo de Clarice Lispector: uma análise comparativa de três obras de Clarice Lispector, traduzidas para o inglês, à luz dos Estudos da Tradução Baseados em Corpus

Pós-graduação em Estudos Linguísticos - IBILCE

Análise de similaridades e diferenças no uso de marcadores de reformulação e frases lexicais, em um corpus paralelo, constituído de contos e romances de Claice Lispector, e as respectivas traduções para o inglês e italiano

Pós-graduação em Estudos Linguísticos - IBILCE

Literatura e leitura: o corpus de trabalho ou o que ensinar em literatura?

Com base nas considerações de alguns críticos literários reconhecidamente empenhados com o problema do ensino da literatura, notadamente Antonio Candido, o presente texto propõe uma breve reflexão sobre o corpus literário a ser adotado em sala-de-aula pelo professor do ensino médio. Em outros termos, trata-se de uma busca, não propriamente de respostas mas de subsídios teóricos, capazes de embasar as reflexões daqueles que se perguntam sobre o que ler, ou o que ensinar em literatura.Palavras-chave: Literatura; ensino; leitura; teoria literária.

O corpus tibullianum: uma análise quantitativa

A partir dos dados extraídos do Corpus Tibullianum, e submetidos a tratamento estatístico, os autores pretendem reforçar a opinião geralmente aceita de que a obra não foi composta por um único poeta, admitindo a pluralidade de autores. Para tanto, utilizam, a distribuição de Waring e os índices mais recentes de riqueza lexical, apontando, entre as duas partes em que dividiram o Corpus, diferenças que corroboram a hipótese inicial.

Morphometric and Functional Characteristics of Buffaloes and Cows Corpus Luteum at Different Stages of the Estrous Cycle

The aims of the present study were to evaluate the morphometry of corpus luteum (CL) and progesterone (P-4) plasma concentration of 86 buffaloes (33 pregnant and 53 non-pregnant) and 95 cows (36 pregnant and 59 non-pregnant) at the moment of slaughter. Seventy CLs of buffaloes and 110 CL of cattle were analyzed. The CL classified as II and III were more common in both species (35.7 and 41.4% for buffaloes and 43.6 and 35.5% for cows). The 29 non-pregnant buffaloes had a total of 36 CL, being 19.4% CLI; 33.3% CL II; 27.8% CL III and 19.4% CL IV. The 51 nonpregnant cows had a total of 71 CL, being 26.8% CL I; 47.9% CL II; 21.1% CL III and 4.2% CL IV. The average diameters of bubaline and bovine CL were 5.2 +/- 0.9 and 6.4 +/- 1.8 mm (CL I); 17.6 +/- 2.6 and 19.8 +/- 3.2 mm (CL II); 17.2 +/- 2.1 and 20.0 +/- 3.2 mm (CL III); 7.8 +/- 1.8 and 8.7 +/- 2.7 mm (CL IV), respectively. The mean plasma concentrations of P4 were 5.6 (CL I); 5.4 (CL II); 4.7 (CL III) and 0.5 (CL IV) ng/mL for buffaloes and 0.02 (CL I); 6.3 (CL II) and 6.4 (CL III) ng/mL for cows. In both species, P4 concentration was similar between stages II and III. The results indicated that the characterization of the CL provides important information about the status of estrous cycle.

O aspecto verbal em Apolodoro: um estudo baseado em corpus sobre os padrões de usos aspectuais em Biblioteca

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A tradução de termos simples, expressões fixas e semifixas em um corpus paralelo da área de sensoriamento remoto: uma proposta de glossário bilíngue

Pós-graduação em Estudos Linguísticos - IBILCE

Estudo da tradução de colocações especializadas na área de exportação e agronegócios baseado em um corpus paralelo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo sobre a tradução de marcadores culturais em três obras de Milton Hatoum com base em corpus paralelo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)