Manejo da fertirrigação para melancia em função da condutividade elétrica da solução do solo

Pós-graduação em Agronomia (Horticultura) - FCA

Microencapsulação por gelificação iônica e interação eletrostática do corante de buriti (Mauritia flexuosa L. f.)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização de Filmes a-C:H:Cl e a-C:H:Si:Cl produzidos por deposição à vapor químico assistido por plasma (PECVD) e deposição e implantação iônica por imersão em plasma (PIIID)

Pós-graduação em Ciência e Tecnologia de Materiais - FC

Emprego de carvão ativado e resinas de troca ionica na remoção de substâncias orgânicas contaminantes do alcool etílico

This work had with objective to characterize and evaluate the performance of the combined system, involving activated charcoal and ionic exchange resins in the removal of substances organic contaminating of the ethyl alcohol from the fermentation of the cassava starch. To testing was used ethyl alcohol PA 96 oGL, conductivity of 0.90 μS/cm at 25 oC, acidity of 60 mg/L and Barbet test of 43 minutes at 15 oC. The contaminated alcohol was composed of ethyl alcohol additive of higher alcohols, organic acids, ester, diol, aldehydes, ketone and ether. Contaminated alcohol was added 2% activated charcoal and after the adsorption isotherm in ionic exchange resins was tested. The adsorption with activated charcoal was performed in a Water Bath at 30 oC for one hour and a half and shaking. Already adsorption ionic exchange columns occurred at room temperature on columns of 93,4 cm in filling height and diameter of 2.29 cm, for flows of 180 mL/min and 90 mL/min. Samples were collected in the tests with charcoal and with each one of the resins and the following analyses were performed: conductivity, acidity and Barbet test. The medium values for conductivity, acidity and Barbet test after the adsorption in charcoal and cation and anion resins were respectively: conductivity was 240; 354 and 465 μS/cm to 25 oC; acidity of 1.081; 1.103 and 1.062 mg/L and the Barbet test was 21; 20 and 9 minutes to 15 oC. It was observed that the addition of 2% of activated charcoal and the permanence in the columns of adsorption was not sufficient to remove the organic substances contaminating of the ethyl alcohol.

Investigação da condutividade hidráulica dos solos de uma área de aterro de resíduos sólidos urbanos por meio de ensaios de campo e laboratório

A contaminant plume due to leachate infiltration was found in the solid waste landfill from Bauru, SP. The soil hydraulic conductivity (K) is an important parameter to understand the moving of this plume. This paper intends to show representative K values for the soils that occur in the vicinity of this landfill determined by field and laboratory tests. There are four soil types in the area: colluvium, alluvium, sandstone residual soil and the sandstone. Laboratory tests were performed using constant and variable head in rigid and flexible wall permeameters. Slug tests and hydraulic conductivity tests with the Guelph permeameter were also carried out in situ in the area. Representative K values of 3.7x10-7 m/s for the saturated zone and 2.4x10-8 m/s for the unsaturated zone were determined for the residual soil. A K value of 5.3x10-5 m/s was obtained for the colluvium soil. A great variation of K with depth was observed for the alluvium and the assumed average values for the saturated zone were 7.9x10-7 m/s and 1.1x10-4 m/s for the unsaturated surficial layer. An average K value of 3.3x10-8 m/s was determined in laboratory for the sandstone and it was assumed representative for this material.

Rainwater chemical composition and annual atmosphere depositon in Sorocaba (SP)

This work evaluated the rainwater chemical composition and the annual atmospheric deposition in Sorocaba, (Sao Paulo State), Brazil. One sampling ˜ point was chosen and forty samples were collected between January and December 2006. The analyses were performed for pH, electrical conductivity, sodium, calcium, potassium, magnesium, sulphate, nitrate, phosphate, alkalinity and chloride. The rainwater pH varied from 5.20 to 6.40, being Ca2+ the main ion responsible for controlling the rainwater pH. The ionic concentration decreases in the following order: Ca2+>Na+> K+>Mg2+ for cations and SO2− 4 >HCO− 3 >NO− 3 >Cl−>PO3− 4 for anions. The annual atmospheric deposition appears to be controlled by the following sources: mining activities and cement factories (Ca2+ and HCO− 3 ), agricultural activities (K+, NO− 3 and PO3− 4 ), soil dust (Na+, Mg2+ and HCO− 3 ) and fossil fuel burning (SO2− 4 )

Influências naturais e antrópicas na qualidade da água subterrânea de poços de abastecimento público na área urbana de Marília (SP)

This work evaluated the natural and anthropogenic influences on the quality of groundwater from public supply wells in the urban area of Marília, São Paulo State. Sixteen sampling points were established in the Adamantina Aquifer, Bauru Aquifer System, analyzing the following parameters: electrical conductivity, temperature, pH, total suspended solids, HCO3 - , PO4 3-, SO4 2-, Cl- , F- , N-NO3 - , Ca2+, Na+ , K+ , Mg2+, Si4+, Fe3+ and Al3+. The results indicated that the groundwater in the urban area of Marília has a slightly acid pH and low conductivity, with the ionic composition presenting a low cation and anion concentration and is classified as soft water and calciumbicarbonated water. Natural sources of elements/compounds can be attributed to the dissolution of carbonates during the water/rock interaction, controlling pH, alkalinity and electrical conductivity, and hydrolysis of other mineral constituents of sedimentary rocks from Adamantina Formation, with the exception of quartz. High concentrations of N-NO3 - found in some public supply wells in urban Marilia were due to sewage.

Estudo da distribuição de tamanho e composição iônica de aerossóis e seus efeitos na capacidade de nuclear gotas de nuvens

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Medidas de condutividade A.C. e constante dielétrica na perovskita dupla Ca1.2 La0.8 FeIrO6

This work introduces the results from the performing of impedance spectroscopy on the transition metals oxide Ca1.2La0.8FeIrO6. It was sought to understand the behavior of one sample from its impedance spectra for different AC voltages and temperature values and if an applied external magnetic field at room temperature would cause some change on it. The results revealed that the Ca1.2La0.8FeIrO6 at high temperatures shows conductive and inductive behavior and that the resistance increases with frequency, phenomenon known as Kelvin effect. At 150 K, the spectrum real part no longer consists with the theoretical prediction of Kelvin effect, starting to be influenced by the utilized voltages, condition that inexists on theory. At low temperatures (10, 20, 30 K) it was observed resistive and capacitive behavior, being possible on these conditions, associate to the sample a paralel RC circuit in series with a contact resistance with a fitting from the ZSim software. This fitting allowed the obtaining of capacitance, DC resistance and contact resistance values. The application of a 700G magnetic field at room temperature didn't cause changes on the spectra

Influência do pericarpo no teste de condutividade elétrica em sementes de crambe (Crambe abyssinica HOCHST) submetidas a métodos de secagem.

In this study, we aimed to evaluate the effect of five drying systems on the quality of crambe seed and the influence of the pericarp in its electrical conductivity. The experimental design adopted was randomized blocks with four replications. The following drying methods were applied: drying in the field, on the patio, under shade, with heated air, and non-heated air. To evaluate the electrical conductivity and the fat acidity, the seeds, from the cultivar FMS Brilhante, were analyzed with and without pericarp. . An analysis of variance was performed and the means were compared by Tukey test (p≤0,05). We also performed the Pearson linear correlation between electrical conductivity and fat acidity in order to evaluate the pericarp influence. The 4 drying system using shades cause less damage to the seeds; and the presence of pericarp decreased the sensitivity of the electrical conductivity

Acúmulo de micronutrientes em beterraba submetida a diferentes manejos de fertirrigação e niveis de salinidade

Knowledge of the amount of nutrients accumulated in the plant provides important information that can assist in the fertilization of crops program. To study the salt effect caused by high amounts of fertilizers applied by fertigation, an experiment was conducted in a greenhouse at the FCA/UNESP, Botucatu, SP. The experiment lasted 90 days (01/11/11 to 29/01/12) constituting five levels of electrical conductivity (1.0, 3.0, 6.0, 9.0, and 12.0 dS m-1), fertigation two management (M1: traditional management, M2: management by controlling the ionic concentration of the soil solution) and two varieties of beets (Early Wonder and Itapuã) blocks with 4 repeats forming a 5x2x2 factorial. From the dry weight of shoots and roots was determined the levels of micronutrients (B, Cu, Fe, Mn, Zn mg kg-1), and by multiplying the dry matter accumulation was determined in these plant . The accumulation of micronutrients in different parts of the plant followed the order: Fe> Mn> Zn> B> Cu for the different management fertigation studied. The Cu, Fe, Mn element present responses to increased soil salinity leading to greater absorption.

Teste de condutividade elétrica para avaliação da qualidade fisiológica de sementes de gergelim (Sesanun indicum L.)

Two experiments were conducted in order to study the effects of seed number, temperature and time of soaking sesame seeds on the results of the electrical conductivity test for physiological quality of seeds. In the experiment I evaluated the electrical conductivity varying the number of seeds (25, 50 and 100) and temperature (20, 25 and 30° C) in experiment II is different soaking periods (2, 4, 6, 8, 12, 16, 20 and 24 h). The tests were performed with three cultivars (cv. Trebol, cv. Cnpa G4 e cv. Comum). The treatments in factorial 3x3x3 and 3x8 in experiments I and II, respectively. The experimental design was completely randomized, with four replications. Means were compared by Tukey test at 5% probability. The characterization of cultivars held through the germination and vigor tests (physical, physiological and biochemical), which rated the Trebol cultivar seeds with better physiological then the Cnpa G4 and Comum. The electrical conductivity test was conducted with four sub-samples of 25 pure seeds soaked in 75 mL of deionized water at 25° C, to separate the lots from 2 hours of soaking, being feasible to evaluate the quality sesame seeds.

Condutividade elétrica de sementes de camomila em resposta ao tempo de embebição e tamanho da amostra

Electrical conductivity has been proposed as a rapid test to evaluate seed vigor; however, few researches have emphasized methodologies to its use in seeds of medicinal plants, such as chamomile. The objective of the research was to evaluate the electrical conductivity of chamomile seeds affected by different imbibition times and sample size. The evaluations consisted of moisture content, germination and vigor (first count of germination) to seed initial characterization. Then, it was evaluated the electrical conductivity, affected by imbibition time (6, 12, 24 e 48 hours) and seed amount per sample (25, 50, 75, 100). The completely randomized design was used with four replications, arranged as a 4 x 4 factorial. Means were compared by the Tukey test at 5% of probability. It was concluded that the electrical conductivity of chamomile seeds is affected by the number of seeds per sample and imbibition time isolately.

Filmes finos depositados pela técnica de implantação iônica por imersão em plasma e deposição (IIIPD), utilizando o monômero HMDSN e os gases argônio, hélio e nitrogênio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estructura de una red esencial de señalización que regula la homeostasis iónica en plantas

Las plantas son organismos sésiles que han desarrollado la capacidad para detectar variaciones sutiles en su ambiente y producir respuestas adaptativas mediante rutas de señalización. Los estímulos causados por el estrés biótico y abiótico son numerosos y dependiendo del tiempo de exposición y su intensidad, pueden reducir la tasa de crecimiento de las plantas y la producción. Los cambios en la concentración del calcio citosólico libre constituyen una de las primeras reacciones intracelulares a las situaciones de estrés abiótico. En esta situación, el calcio actúa como segundo mensajero y las variaciones en su concentración son descodificadas por proteínas de unión a calcio. Las más conocidas son las manos-EF y los dominios C2. Los dominios C2 han sido descritos como dominios de unión a lípidos dependientes de calcio. Estos dominios se consideran proteínas periféricas solubles en agua que se asocian de manera reversible a los lípidos de la membrana mediante una o dos regiones funcionales: el sitio de unión a calcio y el sitio polibásico. A pesar de que se conoce la estructura molecular de algunos dominios C2, se desconocen aspectos relacionados como las reglas que dirigen su forma de interaccionar con los diferentes fosfolípidos y proteínas, la posición que ocupan en la bicapa lipídica y su papel en la transmisión de señales. En esta tesis se ha estudiado una proteína de Arabidopsis thaliana (At3g17980) representativa de una nueva familia de proteínas con dominios C2, que consiste únicamente de un dominio C2. Esta proteína, llamada AtC2.1, ha sido clonada en el vector pETM11, expresada en E. coli y purificada a homogeneidad en dos pasos cromatográficos. Se obtuvieron cristales de AtC2.1 de buena calidad mediante técnicas de difusión de vapor. La proteína fue co-cristalizada con calcio, fosfocolina (POC) y el fosfolípido 1,2-dihexanoil-sn-glicero-3-fosfo-L-serina (PSF). Se recogieron ocho conjuntos de datos de difracción de rayos X empleando radiación sincrotrón. Los cristales difractaron hasta 1.6 Å de resolución. Siete de ellos pertenecían al grupo ortorrómbico P212121, con las dimensiones de la celdilla unidad a = 35.3, b = 88.9, c = 110.6 Å, y un cristal pertenecía al grupo espacial monoclínico C2, con a = 124.84, b = 35.27, c = 92.32 Å y = 121.70º. La estructura se resolvió mediante la técnica MR-SAD utilizando el cinc como dispersor anómalo. La estructura cristalina mostró que la molécula forma un dímero en el que cada protómero se pliega como un dominio C2 típico, con la topología tipo II y presenta una inserción de 43 aminoácidos que la diferencia de los dominios C2 conocidos. El mapa de densidad electrónica mostró dos átomos de calcio por protómero. Se resolvieron las estructuras de AtC2.1 en complejo con POC o PSF. En ambos complejos, el análisis cristalográfico detectó máximos de densidad electrónica en la región correspondiente al sitio polibásico formado por las hebras 2, 3 5 y el lazo 3. Éstos se interpretaron correctamente como dos moléculas de POC y un átomo de cinc, en un complejo, y como la cabeza polar del PSF en el otro. AtC2.1 define un sitio de interacción con lípidos dependiente de cinc. En conclusión, en este trabajo se presenta la estructura tridimensional de AtC2.1, miembro representativo de una familia de proteínas de Arabidopsis thaliana, identificadas como proteínas que interaccionan con los receptores de ABA. Estas proteínas están constituidas únicamente por un dominio C2. El análisis conjunto de los datos biofísicos y cristalográficos muestra que AtC2.1 es un sensor de calcio que une lípidos usando dos sitios funcionales. Estos datos sugieren un mecanismo de inserción en membrana dependiente de calcio que trae consigo la disociación de la estructura dimérica y, por consiguiente, un cambio en las propiedades de superficie de la molécula. Este mecanismo proporciona las bases del reconocimiento y transporte de los receptores de ABA y/o otras moléculas a la membrana celular. Plants are sessile organisms that have developed the capacity to detect slight variations of their environment. They are able to perceive biotic and abiotic stress signals and to transduce them by signaling pathways in order to trigger adaptative responses. Stress factors are numerous and, depending on their exposition time and their concentration, can reduce plant growth rate, limiting the productivity of crop plants. Changes in the cytosolic free calcium concentration are observed as one of the earliest intracellular reactions to abiotic stress signals. Calcium plays a key role as a second messenger, and calcium concentration signatures, called calcium signals, are decodified by calcium binding proteins. The main calcium binding structures are the EF-hand motif and the C2 domains. C2 domain is a calcium dependent lipid-binding domain of approximately 130 amino acids. C2 domain displays two functional regions: the Ca-binding region and the polybasic cluster. Both of them can interact with the membrane phospholipids. Despite the number of C2 domain 3D structures currently available, questions about how they interact with the different target phospholipids, their precise spatial position in the lipid bilayer, interactions with other proteins and their role in transmitting signals downstream, have not yet been explored. In this work we have studied an uncharacterized protein from Arabidopsis thaliana (At3g17980) consisting of only a single C2 domain, as member of a new protein C2-domain family. This protein called AtC2.1 was cloned into the pETM11 vector and expressed in E. coli, allowing the purification to homogeneity in two chromatographic steps. Good quality diffracting crystals were obtained using vapor-diffusion techniques. Crystals were co-crystalized with calcium; phosphocholine (POC) and/or the phospholipid 1,2-dihexanoyl-sn-glycero-3-phospho-L-serine (PSF). Eight data set were collected with synchrotron radiation. Crystals diffracted up to 1.6 Å resolution and seven of them belong to the orthorhombic space group P212121, with unit-cell parameters a = 35.3, b = 88.9, c = 110.6 Å. Another crystal was monoclinic, space group C2, with a = 124.84, b = 35.27, c = 92.32 Å and = 121.70º. The structural model was solved by MR-SAD using Zn2+ as anomalous scatterer. The crystal structure shows that the molecule is a dimer. Each monomer was folded as a canonical C2 domain with the topology II with a 43 residues insertion. The electron density map reveals two calcium ions per molecule. Structures of AtC2.1, complexed with POC and PSF, have been solved. Well-defined extra electron densities were found, in both complexes, within the concave surface formed by strands 2, 3, 5 and loop 3 of AtC2.1. These densities were clearly explained by the presence of the two POC molecules, one zinc atom and head groups of PSF, occupying the cavity of the polybasic site. AtC2.1 defines a new metal dependent lipid-binding site into the polybasic site. In conclusion, in this thesis it is presented the molecular structure of AtC2.1, a representative member of a family of Arabidopsis thaliana C2 domain proteins, of unknown function, but identified as a molecular interacting unit of the ABA receptors. The joint analyses of the biophysical and crystallographic data show that AtC2.1 is a calcium sensor that binds lipids in two sites and suggest a model of calcium-dependent membrane insertion mechanism that will involve either dimer dissociation or a strong rearrangement of the dimeric structure. This mechanism may be the basis for the recognition and delivery of ABA receptors or other protein molecules to cell membranes.