Amostragem para diagnose do estado nutricional e avaliação da fertilidade do solo em caramboleiras

Para a realização de uma análise confiável, a etapa mais sensível e que merece extremo cuidado é a amostragem do tecido vegetal e do solo. A amostra mais adequada é aquela que representa o melhor possível a área de estudo, exigindo um mínimo de plantas amostradas para atender a esse objetivo e com o menor número possível de amostras simples coletadas. Assim, o presente trabalho procurou dimensionar o número de plantas a serem amostradas para a diagnose do estado nutricional, bem como o número de amostras simples necessárias para formar a amostra composta, para fins de avaliação da fertilidade do solo cultivado com caramboleiras. O estudo foi realizado em um pomar comercial de caramboleiras, no município de Vista Alegre do Alto (SP), empregando-se amostragem aleatória, coletando-se a sexta folha a partir do ápice do ramo da caramboleira, na altura mediana da frutífera, no florescimento da cultura, em 40 plantas. Foram coletadas, também, 30 amostras simples de solo, em zigue-zague, nas linhas da cultura, com o auxílio de um trado tipo holandês, nas camadas de 0 a 0,2 m e 0,2 a 0,4 m. Considerando-se aceitável um erro amostral de 10%, 21 plantas de carambola seriam suficientes para as determinações químicas foliares de macronutrientes. Já para os micronutrientes, seriam necessárias, no mínimo, 52 plantas amostradas. O aumento do número de amostras simples reduziu o erro porcentual na estimativa da média desejada, permitindo a recomendação de 14 e 17 amostras simples nas camadas de 0 a 0,2 m e 0,2 a 0,4 m (erro = 20%), respectivamente.

Aclimatização de mudas micropropagadas de bananeira 'Grand Naine' com aplicação de biofertilizantes em duas estações do ano

A utilização de mudas de bananeira micropropagadas de qualidade é o primeiro passo na implantação de um bom pomar e, para isso, sua aclimatização deve ser realizada de forma adequada. em vista disso, objetivou-se, neste trabalho, avaliar o desempenho dos biofertilizantes HUMITEC® e RUTER AA® no desenvolvimento de mudas de bananeira micropropagadas, sendo instalado um experimento em viveiro comercial de produção de mudas. Foram utilizadas mudas de bananeira cv. Grand Naine, obtidas por micropropagação, sendo, posteriormente, transplantadas em sacos de polietileno preto (1,5 L), contendo, como substrato, terra de subsolo (Latossolo Amarelo), casca de arroz carbonizada e composto orgânico Organifol® (1:1:1). O delineamento experimental foi em blocos ao acaso, fatorial 2 x 4 (produtos e doses), com quatro repetições e quatro plantas úteis por parcela. Os tratamentos foram compostos pelos seguintes biofertilizantes e doses: HUMITEC® (0,0; 4,0; 8,0; 16,0 mL planta-1) e RUTER AA® (0,0; 2,0; 4,0; 8,0 mL planta-1). As doses dos produtos foram divididas em duas aplicações (28 e 56 dias após o transplantio das mudas), aplicadas via fertirrigação. Noventa e oito dias após o transplantio avaliaram-se as seguintes variáveis: altura das plantas (cm), diâmetro do colo rente superfície do solo (mm), área foliar total e por folha, biomassas fresca e seca da parte aérea e das raízes (g). Os dados foram submetidos análise da variância e quando o teste F foi significativo, realizou-se análise de regressão. Pode-se concluir que aplicações de HUMITEC® e RUTER AA® favoreceram o desenvolvimento das mudas e que, no verão, as plantas apresentaram maior desenvolvimento durante a aclimatização.

Adubação nitrogenada e substratos na produção de mudas de maracujazeiro doce

Com o objetivo de avaliar diferentes substratos e doses de nitrogênio na produção de mudas de maracujazeiro doce, conduziu-se um experimento no Setor de Fruticultura do Departamento de Agricultura da Universidade Federal de Lavras (UFLA). Foram estudadas cinco doses de nitrogênio 0; 400; 800; 1600 e 3200 mg de N/dm³ e duas composições de substratos: A (composto orgânico + areia + solo na proporção de 1:1:3 em volume) e B (Plantmax® + areia + solo na proporção de 1:1:3 em volume ). Foi utilizado o delineamento experimental em blocos casualizados em esquema fatorial 5 x 2, com quatro repetições e cinco plantas por parcela. Foi observado que doses elevadas de adubação de cobertura com N podem promover efeitos depressivos nas mudas. E que o melhor substrato foi o B, contendo: Plantmax® + areia + solo na proporção de 1:1:3 em volume.

Compostagem dos dejetos da bovinocultura de corte: influência do período, do genótipo e da dieta

O objetivo neste estudo foi identificar possíveis alterações na composição e no processo de compostagem dos dejetos produzidos por bovinos Canchim e Nelore em diferentes períodos do confinamento alimentados com diferentes proporções de volumoso e concentrado. O experimento foi conduzido em delineamento inteiramente casualizado em esquema fatorial com parcelas subdivididas no tempo. As parcelas foram compostas por quatro tratamentos: dejetos provenientes de duas dietas (40% de volumoso e 60% de concentrado e 60% de volumoso e 40% de concentrado) e dois genótipos (Canchim e Nelore) e as subparcelas pelos três períodos de coleta dos resíduos (início, meio e final). A eficiência do processo de compostagem foi avaliada pelas reduções de volume, sólidos totais, sólidos voláteis, frações fibrosas, carbono orgânico e temperatura, além do número mais provável de coliformes totais e termotolerantes e teores de nitrogênio e macro e microminerais. Não houve efeito do genótipo nem do período de coleta dos resíduos sobre a composição dos dejetos, no entanto o aumento da proporção de volumoso na dieta levou a menor eficiência do processo de compostagem dos dejetos. Também foram observadas reduções de 100% no número mais provável de coliformes totais e termotolerantes e aumento no teor dos nutrientes no composto final.

PRODUCTION OF MICROBIAL ALKALINE CELLULASE AND STUDIES OF THEIR CHARACTERISTICS

In order to obtain cellulases that improve the detergency of laundry detergent products, two alkalophilic microorganims, Bacillus sp B38-2 and Streptomyces sp S36-2, were isolated from soil and compost by incubating samples in enrichment culture medium containing CMC and Na2CO3 at pH9.6. It was found that they secrete a constitutive extracellular alkaline carboxymethyl cellulase (CMCase) in high quantity. The maximum enzyme activity was observed between 48hr to 72 hr at 30-degrees-C for the Streptomyces and between 72hr to 96hr at 35-degrees-C for the Bacillus. The optimum pH and temperature of the crude enzyme activities ranged from 6.0 to 7.0 at 55-degrees-C for the Streptomyces and 7.0 to 8.0 at 60-degrees-C for the Bacillus. Two crude CMCases activities were termostable at 45-degrees-C for 1hr and the both crude enzyme activities of the Bacillus as of the Streptomyces were stable at pH 5.0 to 9.0 after pH treatments in various buffer solutions at 30-degrees-C for 24hr.

Assessment of maturity degree of composts from domestic solid wastes by fluorescence and fourier transform infrared spectroscopies

Methods of assessment of compost maturity are needed so the application of composted materials to lands will provide optimal benefits. The aim of the present paper is to assess the maturity reached by composts from domestic solid wastes (DSW) prepared under periodic and permanent aeration systems and sampled at different composting time, by means of excitation-emission matrix (EEM) fluorescence spectroscopy and Fourier transform infrared spectroscopy (FT-IR). EEM spectra indicated the presence of two different fluorophores centered, respectively, at Ex/Em wavelength pairs of 330/425 and 280/330 nm. The fluorescence intensities of these peaks were also analyzed, showing trends related to the maturity of composts. The contour density of EEM maps appeared to be strongly reduced with composting days. After 30 and 45 days of composting, FT-IR spectra exhibited a decrease of intensity of peaks assigned to polysaccharides and in the aliphatic region. EEM and FT-IR techniques seem to produce spectra that correlate with the degree of maturity of the compost. Further refinement of these techniques should provide a relatively rapid method of assessing the suitability of the compost to land application.

Kinetic of thermal decomposition of residues from different kinds of composting

Non-isothermal kinetic parameters regarding to the thermal decomposition of the ligninocellulosic fraction present in compost from urban solid residues (USR) obtained through stack covered (SC) with composted material, comes from the usine in composing of Araraquara city, São Paulo state, Brazil, and from stack containing academic restaurant organic solid residues (SAR). The samples were periodically revolved round 132 days of composting.Results from TG, DTG and DSC curves obtained on inert atmosphere indicated that the lignocellulosic fraction present, despite the slow degradation during the composting process, is thermally less stable than other substances originated during that process. The lignocellulosic fraction decomposition, between 200 and 400degreesC, were kinetically evaluated through non-isothermal methods of analysis.By using the Flynn-Wall and Ozawa isoconversional method, the medium activation energy, E-a, and pre-exponential factor, IgA, were 283.0+/-4.6, 257.6+/-1.3 U mol(-1) and 25.4+/-0.8, 23.2+/-0.2 min(-1),to the SC and SAR, respectively, at 95% confidence level.From E-a, and IgA values and DSC curves, Malek procedure could be applied, Suggesting that the SB (Sestik-Berggren) kinetic model is suitable for the first thermal decomposition step.

Teores de macronutrientes em mudas de aceroleira (Malpighia emarginata DC.) em função da composição do substrato

The composition of substrates with different sources of organic matter is considered as a key factor to obtain seedlings of good quality. Then, this study aimed to evaluate the best source and the best quantity of organic matter that should be used in order to produce barbados cherry (Malpighia emarginata DC.) seedlings of good quality. A factorial arrangement of four (20: 80, 40: 60, 60:40 and 80:20% of organic matter: earth) by four (earthworm compost, burnt rice husk, powdered coconut husk and carnauba straw) levels was used and it was designed in randomized blocks with four replicates. The nitrogen, phosphorus, potassium and calcium content of the leaves and stems were quantified in the dry tissue. The results showed that the proportion 80% of earthworm compost: 20% of earth allowed good development of the plants and sufficient accumulation of N, P, K and Ca. Burnt rice husk, powdered coconut husk and carnauba straw have not generated seedlings of good quality.

Growth, nodulation and nitrogen fixation of cowpea in soils amended with composted tannery sludge

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise físico-química e composição nutricional da matéria prima e de substratos pré e pós cultivo de Pleurotus ostreatus

The aim of the present study was to carry out the physical, chemical and nutritional analyses of the alternative substrates formulated from wood and agroindustrial residues of the Amazon for Pleurotus ostreatus (Jacq.:Fr.) Kummer cultivation. The determination of C, N, pH, humidity, soluble solids, protein, fat, total fiber ash, carbohydrates (total and available) and energy were carried out. The substrates were formulated from sawdust of Simarouba amara Aubl. (marupa), Ochroma piramidale Cav. ex. Lam. (pau de balsa) and from the stein of Bactris gasipaes Kunth (pupunheira palm tree), and from Saccharum officinarum L. (sugar cane bagasse). The results demonstrated that the nutritional composition of the substrate is variable and the improvement of the quality of the spent substrate (energy and protein increment) promoted by the metabolism of the fungus during the cultivation, contributed for a more nutritive substrate than the initial one, which could be used as a compost for Agaricus sp. cultivation, as organic fertilizer and for bioremediation for contaminated soils.

Composto e vermicomposto de lixo urbano na produção de mudas de oiti (Licania tomentosa (Benth)) para arborização

Compost and vermicompost, produced from organic fraction of municipal solid wastes were studied as components of potting media for Licania tomentosa (oiti) seedlings production. The objective was to achieve data to amplify the utilization of these products, in order to utilize them to ornamental and urban arborization species. Increasing rates of organic fertilizers were used until the total soil substitution from the potting media. There were no fitotoxic effects. Compost or vermicompost caused higher germination index of seeds and higher growth of plants. The study demonstrated that it is possible to utilize these products in other activities beyond aplication in agricultural soil, and it can generate new markets to this kind of organic fertilizer.

Maturity degree of composts from municipal solid wastes evaluated by differential scanning calorimetry

Differential scanning calorimetry (DSC) in association with chemical analysis was applied to assess the maturity reached by the organic fraction of Municipal Solid Wastes (MSW) subjected to composting processes with manual and fixed aeration and sampled at different composting times. Thermograms showed that the difference in the treatments, i.e., the manual aeration and the fixed aeration, had no relevant effect on the stabilization and maturation of OM in the substrates. Common thermal effects observed were: a low temperature endotherm assigned to dehydration and/or loss of peripheral polysaccharides chains; a medium temperature exotherm assigned to loss of peptidic structures, and a high temperature exotherm assigned to oxydation and polycondensation of aromatic nuclei of the molecule. Results obtained suggest that in the experimental conditions used, a shorter time of composting (about 30 d) appears adequate, in order to limit the extended mineralization of OM, whereas a prolonged composting time (up to 132 d) would produce a compost of poor quality with high ash content and low OM content.

Produção de composto orgânico a partir de residuos de plantas medicinais

Extraction of active principles from medicinal plants generates wastes usually discharged. This process does not extract minerals from plant tissues, which may be considered of great value in organic composting. Wastes of 81 medicinal plant species processed at Centroflora/Anidro industry were analyzed for macro and micronutrients, and according to the results, three different ways to elaborate the compost were proposed: a) wastes of medicinal plants with high content of a specific mineral, aiming to fertilize soils where would be cultivated species that need high quantities of that mineral; b) wastes with mineral contents in order to support specific failures on mineral content of a soil; c) wastes of the eleven richest species in each nutrient that should elevate all mineral contents in soil.

Heavy metal nutrients in tomato plants cultivated in soil amended with biosolid composts

The use of biosolids in horticulture could contribute to recycle residues produced by men. This study analyzed concentrations of Cu, Mn and Zn in the compost during fermentation, in the soil amended with the composts and in the tomato plant materials. Five composts were produced using sugar-cane bagasse, biosolid and cattle manure in the proportions: 75-0-25; 75-12.5-12.5; 75-25-0; 50-50- 0 and 0-100-0 (composts with 0; 12.5; 25; 50 and 100% biosolid), respectively. These composts were used in an experiment with 6 treatments (the 5 composts and a control with mineral fertilization) in a design of randomized blocks with a split plot design. The control and the treatment of 0% biosolid received inorganic nitrogen. All the treatments received the same amount of N, P and K. Two tomato plants were cultivated in each 24 L pot, in a greenhouse at the Technology Department of the Faculdade de Ciências Agrárias e Veterinárias of the Universidade Estadual Paulista in Jaboticabal County, São Paulo State, Brazil. The concentrations of Cu, Mn and Zn were evaluated in the compost 7, 27, 57, 97 and 127 days after composting began, in the soil 0 and 164 days after the compost applied, and in the plants. Compost, soil and plant samples were subjected to digestion with HNO3, H 2O2 and HCl and the metals were determined by AAS. There were positive and significant correlations between Mn in the compost and Mn uptake by the plant (0.46 p>0.05), and between Zn in the compost and Zn concentration in the plant (0.78 p>0.05). Cu, Mn and Zn concentrations increased during composting. The biosolid in the compost supplied Cu and Zn to tomato plants, and the cattle manure supplied Mn to the plants.

Farming technology, biochemistry characterization, and protective effects of culinary-medicinal mushrooms Agaricus brasiliensis S. Wasser et al. and Lentinus edodes (Berk.) Singer: Five years of research in Brazil

The biotechnology, biochemical characterization, and protective effects of the himematsutake and shiitake mushrooms were studied for the Thematic Project, from 1998 to 2003. A new species, Agaricus Brasiliens is Wasser et al. (= A. blazei Murrill ss. Heinem.), was proposed for the cultivated lineages in Brazil. Interactions among lineages, substrates, casing layers, and fructification inductions (temperature alternations) significantly increased the productivity of A. brasiliensis in Brazil (from 40 g to 200 g of fresh mushroom kg -1 moist compost). However, pests and diseases (mainly Sciaridae flies and false truffle), drastically reduced the productivity of A. brasiliensis (below 50 g kg -1). Biochemically for each mushroom species, the polar extracts, no polars, and medium polarity presented the same organic substances; however, their concentrations differed as a result of the lineages, maturation of the fruit bodies, and cultivation conditions. In this aspect, concentration of linoleic acid in A. brasiliensis (added to animal food) was related directly to the chemical protection against carcinogenic drugs in mice. Aqueous extracts of Lentinus edodes (= Lentinula edodes (Berk.) Pegler) and A. brasiliensis may be preventive chemical protectors against mutagenic and carcinogenic drugs, depending on the lineage and extraction method (tea or juice). However, immunomodulator effects and tumor reduction were only observed with concentrated fractions (hexanic, methanolic, and ATF extracts). Aqueous extracts of A. brasiliensis and L. edodes have antibiotic-like substances and substances able to act as elicitors of resistance responses in some plants (local and systemic) and show a potential to be used in the alternative control of plant pathogens. © 2005 by Begell House, Inc.