Fabrication of 25nm wide gold lines using nanometer scale lithography and ionized cluster beam deposition

It becomes increasingly difficult to make continuous metal lines with well defined thickness and edges by the lift-off technique as the line width is decreased. We describe in this paper a technique in which the combination of high resolution electron beam lithography and ionized cluster beam (ICB) deposition has enabled very high quality gold lines ({all equal to}25nm wide) to be obtained on thick single crystal silicon substrates. © 1990.

STREAM Initiative report to the 8th NACA Technical Advisory Committee Meeting. Tehran, Iran 1-3 October 2005

The STREAM Initiative set logframe indicators, at their Technical Advisory Committee meetings and this is a progress report based on given indicators. It further includes two articles: The Kandhkelgaon story: A bold bid by women in Kandhkelgaon Village, Saintala Block, Bolangir District, to break out of their poverty trap, by Graham Haylor, S.D. Tripathi, B.K. Satpathy and Dipti Behera. Networking for rural development: a closer look at the evolution of communications in the STREAM Initiative, by Graham Haylor, Kath Copley and William Savage. (PDF contains 27 Pages)

An examination of strategic opportunities provided by the conference committee procedure in the U.S. Congress

Deference to committees in Congress has been a much studied phenomena for close to 100 years. This deference can be characterized as the unwillingness of a potentially winning coalition on the House floor to impose its will on a small minority, a standing committee. The congressional scholar is then faced with two problems: observing such deference to committees, and explaining it. Shepsle and Weingast have proposed the existence of an ex-post veto for standing committees as an explanation of committee deference. They claim that as conference reports in the House and Senate are considered under a rule that does not allow amendments, the conferees enjoy agenda-setting power. In this paper I describe a test of such a hypothesis (along with competing hypotheses regarding the effects of the conference procedure). A random-utility model is utilized to estimate legislators' ideal points on appropriations bills from 1973 through 1980. I prove two things: 1) that committee deference can not be said to be a result of the conference procedure; and moreover 2) that committee deference does not appear to exist at all.

Design of a Recreational Fishing Survey and Mark-Recapture Study for the Blue Crab, Callinectes sapidus, in Chesapeake Bay

The development of bay wide estimates of recreational harvest has been identified as a high priority by the Chesapeake Bay Scientific Advisory Committee (CBSAC) and by the Chesapeake Bay Program as reflected in the Chesapeake Bay Blue Crab Fishery Management Plan (Chesapeake Bay Program 1996). In addition, the BiState Blue Crab Commission (BBCAC), formed in 1996 by mandate from the legislatures of Maryland and Virginia to advise on crab management, has also recognized the importance of estimating the levels and trends in catches in the recreational fishery. Recently, the BBCAC has adopted limit and target biological reference points. These analyses have been predicated on assumptions regarding the relative magnitude of the recreational and commercial catch. The reference points depend on determination of the total number of crabs removed from the population. In essence, the number removed by the various fishery sectors, represents a minimum estimate of the population size. If a major fishery sector is not represented, the total population will be accordingly underestimated. If the relative contribution of the unrepresented sector is constant over time and harvests the same components of the population as the other sectors, it may be argued that the population estimate derived from the other sectors is biased but still adequately represents trends in population size over time. If either of the two constraints mentioned above is not met, the validity of relative trends over time is suspect. With the recent increases in the human population in the Chesapeake Bay watershed, there is reason to be concerned that the recreational catch may not have been a constant proportion of the total harvest over time. It is important to assess the catch characteristics and the magnitude of the recreational fishery to evaluate this potential bias. (PDF contains 70 pages)

Innovations of wide-field optical-sectioning fluorescence microscopy : toward high-speed volumetric bio-imaging with simplicity

Optical microscopy has become an indispensable tool for biological researches since its invention, mostly owing to its sub-cellular spatial resolutions, non-invasiveness, instrumental simplicity, and the intuitive observations it provides. Nonetheless, obtaining reliable, quantitative spatial information from conventional wide-field optical microscopy is not always intuitive as it appears to be. This is because in the acquired images of optical microscopy the information about out-of-focus regions is spatially blurred and mixed with in-focus information. In other words, conventional wide-field optical microscopy transforms the three-dimensional spatial information, or volumetric information about the objects into a two-dimensional form in each acquired image, and therefore distorts the spatial information about the object. Several fluorescence holography-based methods have demonstrated the ability to obtain three-dimensional information about the objects, but these methods generally rely on decomposing stereoscopic visualizations to extract volumetric information and are unable to resolve complex 3-dimensional structures such as a multi-layer sphere.

The concept of optical-sectioning techniques, on the other hand, is to detect only two-dimensional information about an object at each acquisition. Specifically, each image obtained by optical-sectioning techniques contains mainly the information about an optically thin layer inside the object, as if only a thin histological section is being observed at a time. Using such a methodology, obtaining undistorted volumetric information about the object simply requires taking images of the object at sequential depths.

Among existing methods of obtaining volumetric information, the practicability of optical sectioning has made it the most commonly used and most powerful one in biological science. However, when applied to imaging living biological systems, conventional single-point-scanning optical-sectioning techniques often result in certain degrees of photo-damages because of the high focal intensity at the scanning point. In order to overcome such an issue, several wide-field optical-sectioning techniques have been proposed and demonstrated, although not without introducing new limitations and compromises such as low signal-to-background ratios and reduced axial resolutions. As a result, single-point-scanning optical-sectioning techniques remain the most widely used instrumentations for volumetric imaging of living biological systems to date.

In order to develop wide-field optical-sectioning techniques that has equivalent optical performance as single-point-scanning ones, this thesis first introduces the mechanisms and limitations of existing wide-field optical-sectioning techniques, and then brings in our innovations that aim to overcome these limitations. We demonstrate, theoretically and experimentally, that our proposed wide-field optical-sectioning techniques can achieve diffraction-limited optical sectioning, low out-of-focus excitation and high-frame-rate imaging in living biological systems. In addition to such imaging capabilities, our proposed techniques can be instrumentally simple and economic, and are straightforward for implementation on conventional wide-field microscopes. These advantages together show the potential of our innovations to be widely used for high-speed, volumetric fluorescence imaging of living biological systems.

Wide Field-of-view Microscopes and Endoscopes for Time-lapse Imaging and High-throughput Screening

Wide field-of-view (FOV) microscopy is of high importance to biological research and clinical diagnosis where a high-throughput screening of samples is needed. This thesis presents the development of several novel wide FOV imaging technologies and demonstrates their capabilities in longitudinal imaging of living organisms, on the scale of viral plaques to live cells and tissues.

The ePetri Dish is a wide FOV on-chip bright-field microscope. Here we applied an ePetri platform for plaque analysis of murine norovirus 1 (MNV-1). The ePetri offers the ability to dynamically track plaques at the individual cell death event level over a wide FOV of 6 mm × 4 mm at 30 min intervals. A density-based clustering algorithm is used to analyze the spatial-temporal distribution of cell death events to identify plaques at their earliest stages. We also demonstrate the capabilities of the ePetri in viral titer count and dynamically monitoring plaque formation, growth, and the influence of antiviral drugs.

We developed another wide FOV imaging technique, the Talbot microscope, for the fluorescence imaging of live cells. The Talbot microscope takes advantage of the Talbot effect and can generate a focal spot array to scan the fluorescence samples directly on-chip. It has a resolution of 1.2 μm and a FOV of ~13 mm². We further upgraded the Talbot microscope for the long-term time-lapse fluorescence imaging of live cell cultures, and analyzed the cells’ dynamic response to an anticancer drug.

We present two wide FOV endoscopes for tissue imaging, named the AnCam and the PanCam. The AnCam is based on the contact image sensor (CIS) technology, and can scan the whole anal canal within 10 seconds with a resolution of 89 μm, a maximum FOV of 100 mm × 120 mm, and a depth-of-field (DOF) of 0.65 mm. We also demonstrate the performance of the AnCam in whole anal canal imaging in both animal models and real patients. In addition to this, the PanCam is based on a smartphone platform integrated with a panoramic annular lens (PAL), and can capture a FOV of 18 mm × 120 mm in a single shot with a resolution of 100─140 μm. In this work we demonstrate the PanCam’s performance in imaging a stained tissue sample.

Distortion of beam shape by a rotating double-prism wide-angle laser beam scanner

We describe the rigorous results of a wide-angle laser beam scanner, obtained with the help of the vector refraction theory. Using the rigorous results, the distortion of the beam shape is discussed. The distortion to the beam varies with the different relative angles of double prisms. The scanner expands the beam in some directions while it contracts the beam in other directions. According to the conservation of energy, the distribution of the laser intensity is changed as well. (c) 2006 Society of Photo-Optical Instrumentation Engineers.

Distortion of beam shape by a rotating double-prism wide-angle laser beam scanner

We describe the rigorous results of a wide-angle laser beam scanner, obtained with the help of the vector refraction theory. Using the rigorous results, the distortion of the beam shape is discussed. The distortion to the beam varies with the different relative angles of double prisms. The scanner expands the beam in some directions while it contracts the beam in other directions. According to the conservation of energy, the distribution of the laser intensity is changed as well. (c) 2006 Society of Photo-Optical Instrumentation Engineers.

Eden & District Fisheries Advisory Committee 14th October, 1975

This is the report from the Eden and District Fisheries Advisory Committee meeting, which was held on the 14th October 1975. The report looks at the planning study for the post 1981 period and fisheries activities on the Eden and Esk. This section includes catches of miragtory, brown trout and coarse fish, the summary of the stocking carried out from Holmewrangle hatchery and Biological work undertaken on the Eden. It also contain sections on fish mortalities, new code of fishery byelaws and fishing licence duties. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Mersey & Weaver Fisheries Advisory Committee 22nd October, 1975.

This is the report from the Mersey and Weaver Fisheries Advisory Committee meeting, which was held on the 22nd October 1975. The report contains information on a report by the divisional scientist on fisheries activities, importation of live freshwater fish, drainage representation on local committees, new byelaws and fishing licence duties. The report by the divisional scientist looks at permissions for stocking and electrofishing, biological work and results of fish toxicity tests carried out in Rivers Bollin, Etherow, Glaze and Tame. It also contains records of fish mortalities. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Eden Fisheries Advisory Committee 3rd December, 1974

This is the report from the Eden Fisheries Advisory Committee meeting, which was held on the 3rd December 1974. The report looks at information on the Water Bailiffs establishment, including the organisation and the duties of both the Fisheries Inspector and the Senior Bailiff. It also includes the comments from the Regional Fisheries Officer on the report on 'Coarse Fisheries'. Also covered is the report by the Unit Fisheries Officer on fisheries activities. This includes biological work carried out including information on an invertebrate bottom sampling programme at 50 sites on the Eden and its tributaries, fish mortalities, information on the fishing of migratory fish, trout and coarse fish, hatchery work and fisheries prosecutions. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Lune, Wyre & Furness Fisheries Advisory Committee 9th December, 1974

This is the report from the Lune, Wyre and Furness Fisheries Advisory Committee meeting, which was held on the 9th December 1974. The report looks at information on the Water Bailiffs establishment, including the organisation and the duties of both the Fisheries Inspector and the Senior Bailiff. It also covers the comments from the Regional Fisheries Officer on the report on 'Coarse Fisheries', and the report by the Unit Fisheries Officer on fisheries activities. This looks at coarse fish salvage and stocking, fisheries management, biological work carried out, which include initial studies of the utilisation of sonic tagging methods in the monitoring of salmon in estuaries, fish mortalities, and the fish monitoring figures for areas on the Rivers Lune and Leven. The last section looks at bank erosion on the River Lune. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Eden & District Fisheries Advisory Committee 13th January, 1976.

This is the report from the Eden and District Fisheries Advisory Committee meeting, which was held on the 13th January 1976. The report contains information on the land drainage representation on local committees, fisheries activities report, salmon stocking on the River Eden, status of Haweswater and Castle Carrock Reservoir, fisheries byelaws, licensing provisions for the River Esk and progress made in implementing the recommendations contained in the 'Taking Stock' publication. The section on fisheries activities reported by area fisheries officers looks at fishing, spawning disease and poaching of migratory fish and coarse fishing. It also contains description of ova obtained at Holmwrange hatchery and provisions of new equipment, biological work carried on Eden and Blackrack Beck and suggestions for new fish counters and traps. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

South Lancashire Fisheries Advisory Committee 11th December, 1974

This is the report from the South Lancashire Fisheries Advisory Committee meeting, which was held on the 11th December 1974. The report looks at information on the Water Bailiffs establishment, including the organisation and the duties of both the Fisheries Inspector and the Senior Bailiff. It also covers the comments from the Regional Fisheries Officer on the report on 'Coarse Fisheries', and information on the discharge to the River Ribble from Clitheroe sewage works. Item number 7 looks at the report by the Unit Fisheries Officer on fisheries activities. This looks at coarse fish salvage and stocking, fisheries management, poaching, fish mortalities, and the fish monitoring figures for areas on the Rivers Ribble and Hodder. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

Mersey & Weaver Fisheries Advisory Committee 16th December, 1974

This is the report from the Mersey and Weaver Fisheries Advisory Committee meeting, which was held on the 16th December 1974. The report looks at information on the Water Bailiffs establishment, including the organisation and the duties of both the Fisheries Inspector and the Senior Bailiff. It also covers the comments from the Regional Fisheries Officer on the report on 'Coarse Fisheries', and information on the structure of licence duties in the Mersey and Weaver River unit area. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.