The unidirectional emptying box

A theoretical description of the turbulent mixing within and the draining of a dense fluid layer from a box connected to a uniform density, quiescent environment through openings in the top and the base of the box is presented in this paper. This is an extension of the draining model developed by Linden et al. (Annu. Rev. Fluid Mech. vol. 31, 1990, pp. 201-238) and includes terms that describe localized mixing within the emptying box at the density interface. Mixing is induced by a turbulent flow of replacement fluid into the box and as a consequence we predict, and observe in complementary experiments, the development of a three-layer stratification. Based on the data collated from previous researchers, three distinct formulations for entrainment fluxes across density interfaces are used to account for this localized mixing. The model was then solved numerically for the three mixing formulations. Analytical solutions were developed for one formulation directly and for a second on assuming that localized mixing is relatively weak though still significant in redistributing buoyancy on the timescale of the draining process. Comparisons between our theoretical predictions and the experimental data, which we have collected on the developing layer depths and their densities show good agreement. The differences in predictions between the three mixing formulations suggest that the normalized flux turbulently entrained across a density interface tends to a constant value for large values of a Froude number FrT, based on conditions of the inflow through the top of the box, and scales as the cube of FrT for small values of FrT. The upper limit on the rate of entrainment into the mixed layer results in a minimum time (tD) to remove the original dense layer. Using our analytical solutions, we bound this time and show that 0.2tE ≈tD tE, i.e. the original dense layer may be depleted up to five times more rapidly than when there is no internal mixing and the box empties in a time tE. © 2010 Cambridge University Press.

金鱼DEAD-box家族基因p68和p110在配子发生中的表达特征

利用组织原位杂交技术,以地高辛标记的反义RNA为探针,检测了金鱼(Carassius auratus)DEAD-box家族基因p68和p110在卵子发生及精子发生中的表达分布特点。结果表明:在金鱼配子发生中,p68基因在各个时期的卵母细胞均有表达,Ⅰ、Ⅱ期卵母细胞中的表达水平较Ⅲ、Ⅳ期卵母细胞中更高。p68基因的表达只限于精原细胞和初级精母细胞中。p110基因在金鱼精子发生各阶段持续表达,其中精原细胞和初级精母细胞中表达水平较高;在卵子发生中,Ⅰ期卵母细胞中没有p110 RNA阳性信号,Ⅱ期卵母细胞中信号

黄鳝DEAD-box家族PL10基因的克隆与序列分析

通过PCR克隆的方法,从黄鳝(Monopterus albus)中得到两个PL10 基因的cDNA片段Mo PL10A和Mo PL10B,长度均为1.127 kb,推测其编码375个氨基酸的蛋白片段.结合其他PL10类同源物序列,对这两条cDNA进行了分析和初步的功能推测.根据此片段的氨基酸序列构建的系统发育树与形态分类结果一致.在不同组织中的RT PCR结果表明Mo PL10A和Mo PL10B的mRNA在各组织中的分布有差异.

Parallels between wind and crowd loading of bridges

Parallels between the dynamic response of flexible bridges under the action of wind and under the forces induced by crowds allow each field to inform the other.Wind-induced behaviour has been traditionally classified into categories such as flutter, galloping, vortex-induced vibration and buffeting. However, computational advances such as the vortex particle method have led to a more general picture where effects may occur simultaneously and interact, such that the simple semantic demarcations break down. Similarly, the modelling of individual pedestrians has progressed the understanding of human–structure interaction, particularly for large amplitude lateral oscillations under crowd loading. In this paper, guided by the interaction of flutter and vortexinduced vibration in wind engineering, a framework is presented, which allows various human–structure interaction effects to coexist and interact, thereby providing a possible synthesis of previously disparate experimental and theoretical results.

Predictive entropy search for efficient global optimization of black-box functions

We propose a novel information-theoretic approach for Bayesian optimization called Predictive Entropy Search (PES). At each iteration, PES selects the next evaluation point that maximizes the expected information gained with respect to the global maximum. PES codifies this intractable acquisition function in terms of the expected reduction in the differential entropy of the predictive distribution. This reformulation allows PES to obtain approximations that are both more accurate and efficient than other alternatives such as Entropy Search (ES). Furthermore, PES can easily perform a fully Bayesian treatment of the model hyperparameters while ES cannot. We evaluate PES in both synthetic and real-world applications, including optimization problems in machine learning, finance, biotechnology, and robotics. We show that the increased accuracy of PES leads to significant gains in optimization performance.

Fabrication of nanoscale metallic air-bridges by introducing a SiO2 sacrificial layer

A new method to fabricate nanoscale metallic air-bridges has been investigated. The pillar patterns of the air-bridge were defined on a SiO2, sacrificial layer by electron-beam lithography combined with inductively coupled plasma etching. Thereafter, the span (suspended part between the pillars) patterns were defined with a second electron-beam exposure on a PMMA/PMMA-MAA resist system. The fabrication process was completed by subsequent metal electron-beam evaporation, lift-off in acetone, and removal of the sacrificial layer in a buffered hydrofluoric (HF) solution. Air-bridges with two different geometries (line-shaped and cross-shaped) were studied in detail. The narrowest width of the air-bridges was around 200 nm, and the typical length of the air-bridges was 2-5 mu m. The advantages of our method are the simplicity of carrying out electron-beam exposure with good reproducibility and the capability of more accurate control of the pillar sizes and shapes of the air-bridge. (C) 2007 Elsevier Ltd. All rights reserved.

Fabrication of metallic air bridges using multiple-dose electron beam lithography

The techniques of fabricating metallic air bridges using different resists in a one-step electron beam lithography are presented. The exposure process employed a single-layer polymethyl methacrylate (PMMA) or photoresists with either different doses in the span and feet areas or with varying acceleration voltage of the electron beam. The process using photoresists with different doses has produced air bridges more stable than what the PMMA method using various acceleration voltages would achieve. Using this method, air bridges up to 12 mu m long have been fabricated. The length and height of these metallic air bridges vary with the photoresist thickness. (c) 2006 American Institute of Physics.

High-speed photodetector characterization using tunable laser by optical heterodyne technique - art. no. 60200A

An ultra-wide-band frequency response measurement system for optoelectronic devices has been established using the optical heterodyne method utilizing a tunable laser and a wavelenath-fixed distributed feedback laser. By controlling the laser diode cavity length, the beat frequency is swept from DC to hundreds GHz. An outstanding advantage is that this measurement system does not need any high-speed light modulation source and additional calibration. In this measurement, two types of different O/E receivers have been tested. and 3 dB bandwidths measured by this system were 14.4GHz and 40GHz, respectively. The comparisons between experimental data and that from manufacturer show that this method is accurate and easy to carry out.

High-output very small aperture laser and its near-field distribution properties - art. no. 60200L

A Very-Small-Aperture Laser with a 250 X 500 nm(2) aperture has been created on a 650nm edge emitting LD. The highest far-field output power is 1.9mW and the power per unit emission area is about 15 MW/mu m(2). The special fabrication process and high output power mechanism are demonstrated respectively. The near-field distribution properties are also analyzed theoretically and experimentally.

Electroabsorption-modulated DFB laser integrated with dual-waveguide spot-size converter - art. no. 60200N

A 1.55-mu m ridge DFB laser and electroabsorption modulator monolithically integrated with a buried-ridge-stripe dual-waveguide spot-size converter at the output port for low-loss coupling to a cleaved single-mode optical fiber was fabricated by means of selective area growth, quantum well intermixing and dual-core technologies. These devices exhibit threshold current of 28 mA, side mode suppression ratio of 38.0 dB, 3-dB modulation bandwidth of 12.0 GHz, modulator extinction ratios of 25.0 dB dc. The output beam divergence angles of the spot-size converter in the horizontal and vertical directions are as small as 8.0 degrees x 12.6 degrees, respectively, resulting in 3.2 dB coupling loss with a cleaved single-mode optical fiber.

Tandem electroabsorption modulators integrated with DFB laser by ultra-low-pressure selective-area-growth MOCVD for 10 GHz optical short pulse generation - art. no. 60200O

A novel device of tandem multiple quantum wells (MQWs) electroabsorption modulators (EAMs) monolithically integrated with DFB laser is fabricated by ultra-low-pressure (22 mbar) selective area guowth (SAG) MOCVD technique. Experimental results exhibit superior device characteristics with low threshold of 19 mX output light power of 4.5 mW and over 20 dB extinction ratio when coupled into a single mode Fiber. Moreover, over 10 GHz modulation bandwidth is developed with a driving voltage of 2 V. Using I this sinusoidal voltage driven integrated device, 10GHz repetition rate pulse with a width of 13.7 ps without any compression elements is obtained.

Wavelength-selectable DFB laser with wide gain bandwidth realized in nonidentical quantum well - art. no. 60200W

Width varied quantum wells show a more flat and wide gain spectrume (about 115nm) than that of identical miltiple quantum well. A new fabricating method was demonstrated in this paper to realize two different Bragg grating in an selectable DFB laser based on this material grown identical chip using traditional holographic exposure. A wavelength by MOVPE was presented. Two stable distinct single longitudinal mode of 1510nm and 1530nm with SMSR of 45 dB were realized.