Orographic precipitation in the tropics: experiments in Dominica

The “natural laboratory” of mountainous Dominica (15°N) in the trade wind belt is used to study the physics of tropical orographic precipitation in its purest form, unforced by weather disturbances or by the diurnal cycle of solar heating. A cross-island line of rain gauges and 5-min radar scans from Guadeloupe reveal a large annual precipitation at high elevation (7 m yr^{−1}) and a large orographic enhancement factor (2 to 8) caused primarily by repetitive convective triggering over the windward slope. The triggering is caused by terrain-forced lifting of the conditionally unstable trade wind cloud layer. Ambient humidity fluctuations associated with open-ocean convection may play a key role. The convection transports moisture upward and causes frequent brief showers on the hilltops. The drying ratio of the full air column from precipitation is less than 1% whereas the surface air dries by about 17% from the east coast to the mountain top. On the lee side, a plunging trade wind inversion and reduced instability destroys convective clouds and creates an oceanic rain shadow.

Hydroponic isotope labeling of entire plants (HILEP) for quantitative plant proteomics

Quantitative analysis by mass spectrometry (MS) is a major challenge in proteomics as the correlation between analyte concentration and signal intensity is often poor due to varying ionisation efficiencies in the presence of molecular competitors. However, relative quantitation methods that utilise differential stable isotope labelling and mass spectrometric detection are available. Many drawbacks inherent to chemical labelling methods (ICAT, iTRAQ) can be overcome by metabolic labelling with amino acids containing stable isotopes (e.g. 13C and/or 15N) in methods such as Stable Isotope Labelling with Amino acids in Cell culture (SILAC). SILAC has also been used for labelling of proteins in plant cell cultures (1) but is not suitable for whole plant labelling. Plants are usually autotrophic (fixing carbon from atmospheric CO2) and, thus, labelling with carbon isotopes becomes impractical. In addition, SILAC is expensive. Recently, Arabidopsis cell cultures were labelled with 15N in a medium containing nitrate as sole nitrogen source. This was shown to be suitable for quantifying proteins and nitrogen-containing metabolites from this cell culture (2,3). Labelling whole plants, however, offers the advantage of studying quantitatively the response to stimulation or disease of a whole multicellular organism or multi-organism systems at the molecular level. Furthermore, plant metabolism enables the use of inexpensive labelling media without introducing additional stress to the organism. And finally, hydroponics is ideal to undertake metabolic labelling under extremely well-controlled conditions. We demonstrate the suitability of metabolic 15N hydroponic isotope labelling of entire plants (HILEP) for relative quantitative proteomic analysis by mass spectrometry. To evaluate this methodology, Arabidopsis plants were grown hydroponically in 14N and 15N media and subjected to oxidative stress.

Carbon and nitrogen isotopic ratios of urine and faeces as novel nutritional biomarkers of meat and fish intake

Purpose Meat and fish consumption are associated with changes in the risk of chronic diseases. Intake is mainly assessed using self-reporting, as no true quantitative nutritional biomarker is available. The measurement of plasma fatty acids, often used as an alternative, is expensive and time-consuming. As meat and fish differ in their stable isotope ratios, δ13C and δ15N have been proposed as biomarkers. However, they have never been investigated in controlled human dietary intervention studies. Objective In a short-term feeding study, we investigated the suitability of δ13C and δ15N in blood, urine and faeces as biomarkers of meat and fish intake. Methods The dietary intervention study (n = 14) followed a randomised cross-over design with three eight-day dietary periods (meat, fish and half-meat–half-fish). In addition, 4 participants completed a vegetarian control period. At the end of each period, 24-h urine, fasting venous blood and faeces were collected and their δ13C and δ15N analysed. Results There was a significant difference between diets in isotope ratios in faeces and urine samples, but not in blood samples (Kruskal–Wallis test, p < 0.0001). In pairwise comparisons, δ13C and δ15N were significantly higher in urine and faecal samples following a fish diet when compared with all other diets, and significantly lower following a vegetarian diet. There was no significant difference in isotope ratio between meat and half-meat–half-fish diets for blood, urine or faecal samples. Conclusions The results of this study show that urinary and faecal δ13C and δ15N are suitable candidate biomarkers for short-term meat and fish intake.

Carbon and nitrogen stable isotope ratio analysis of freshwater, brackish and marine fish from Belgian archaeological sites (1st and 2nd millennium AD)

Carbon and nitrogen stable isotope ratios were measured in 157 fish bone collagen samples from 15 different archaeological sites in Belgium which ranged in ages from the 3rd to the 18th c. AD. Due to diagenetic contamination of the burial environment, only 63 specimens produced results with suitable C:N ratios (2.9–3.6). The selected bones encompass a wide spectrum of freshwater, brackish, and marine taxa (N = 18), and this is reflected in the δ13C results (−28.2‰ to −12.9%). The freshwater fish have δ13C values that range from −28.2‰ to −20.2‰, while the marine fish cluster between −15.4‰ and −13.0‰. Eel, a catadromous species (mostly living in freshwater but migrating into the sea to spawn), plots between −24.1‰ and −17.7‰, and the anadromous fish (living in marine environments but migrating into freshwater to spawn) show a mix of freshwater and marine isotopic signatures. The δ15N results also have a large range (7.2‰ to 16.7‰) indicating that these fish were feeding at many different trophic levels in these diverse aquatic environments. The aim of this research is the isotopic characterization of archaeological fish species (ecology, trophic level, migration patterns) and to determine intra-species variation within and between fish populations differing in time and location. Due to the previous lack of archaeological fish isotope data from Northern Europe and Belgium in particular, these results serve as an important ecological backdrop for the future isotopic reconstruction of the diet of human populations dating from the historical period (1st and 2nd millennium AD), where there is zooarchaeological and historical evidence for an increased consumption of marine fish.

The diet-body offset in human nitrogen isotopic values: a controlled dietary study

The ‘trophic level enrichment’ between diet and body results in an overall increase in nitrogen isotopic values as the food chain is ascended. Quantifying the diet–body Δ15N spacing has proved difficult, particularly for humans. The value is usually assumed to be +3-5‰ in the archaeological literature. We report here the first (to our knowledge) data from humans on isotopically known diets, comparing dietary intake and a body tissue sample, that of red blood cells. Samples were taken from 11 subjects on controlled diets for a 30-d period, where the controlled diets were designed to match each individual’s habitual diet, thus reducing problems with short-term changes in diet causing isotopic changes in the body pool. The Δ15Ndiet-RBC was measured as +3.5‰. Using measured offsets from other studies, we estimate the human Δ15Ndiet-keratin as +5.0-5.3‰, which is in good agreement with values derived from the two other studies using individual diet records. We also estimate a value for Δ15Ndiet-collagen of ≈6‰, again in combination with measured offsets from other studies. This value is larger than usually assumed in palaeodietary studies, which suggests that the proportion of animal protein in prehistoric human diet may have often been overestimated in isotopic studies of palaeodiet.

Serum carbon and nitrogen stable isotopes as potential biomarkers of dietary intake and their relation with incident type 2 diabetes: the EPIC-Norfolk study

Background: Stable-isotope ratios of carbon (13C/12C, expressed as δ13C) and nitrogen (15N/14N, or δ15N) have been proposed as potential nutritional biomarkers to distinguish between meat, fish, and plant-based foods. Objective: The objective was to investigate dietary correlates of δ13C and δ15N and examine the association of these biomarkers with incident type 2 diabetes in a prospective study. Design: Serum δ13C and δ15N (‰) were measured by using isotope ratio mass spectrometry in a case-cohort study (n = 476 diabetes cases; n = 718 subcohort) nested within the European Prospective Investigation into Cancer and Nutrition (EPIC)–Norfolk population-based cohort. We examined dietary (food-frequency questionnaire) correlates of δ13C and δ15N in the subcohort. HRs and 95% CIs were estimated by using Prentice-weighted Cox regression. Results: Mean (±SD) δ13C and δ15N were −22.8 ± 0.4‰ and 10.2 ± 0.4‰, respectively, and δ13C (r = 0.22) and δ15N (r = 0.20) were positively correlated (P < 0.001) with fish protein intake. Animal protein was not correlated with δ13C but was significantly correlated with δ15N (dairy protein: r = 0.11; meat protein: r = 0.09; terrestrial animal protein: r = 0.12, P ≤ 0.013). δ13C was inversely associated with diabetes in adjusted analyses (HR per tertile: 0.74; 95% CI: 0.65, 0.83; P-trend < 0.001], whereas δ15N was positively associated (HR: 1.23; 95% CI: 1.09, 1.38; P-trend = 0.001). Conclusions: The isotope ratios δ13C and δ15N may both serve as potential biomarkers of fish protein intake, whereas only δ15N may reflect broader animal-source protein intake in a European population. The inverse association of δ13C but a positive association of δ15N with incident diabetes should be interpreted in the light of knowledge of dietary intake and may assist in identifying dietary components that are associated with health risks and benefits.

Isotopic examination of links between diet, social differentiation and DISH at the post-medieval Carmelite Friary of Aalst, Belgium

Stable isotope ratios (δ13C and δ15N) were measured in human burials from the post-medieval (16th–18th c. AD) Carmelite friary burial grounds at Aalst, a town in Flanders, Belgium. Dietary patterns of 39 adult individuals were analyzed, from a mixed monastic and lay population buried in three different locations, reflecting groups with differing social status. The data show significant variation in the consumption of perhaps meat, but certainly also marine protein between females and males. This result represents a remarkable continuity with medieval dietary patterns, suggesting that the social and economic changes of the early modern period had a limited effect on everyday life. When both sexes were examined together, individuals buried in the cloister garth consumed significantly less marine protein compared to people buried in the church, likely reflecting social stratification. No statistical differences were observed between isotopic values from the church and the cloister alley, suggesting a similarly diverse diet of the monastic part of the buried population and that of the richer lay population. Finally, the hypothesis that diffuse idiopathic skeletal hyperostosis (DISH) is linked to a diet rich in animal protein was tested. No systematic or statistically significant differences between pathological and non-pathological bones from the same individuals affected with DISH were observed, and no statistical differences were found between individuals with DISH and individuals without DISH

Mercury and methylmercury bioaccumulation by polychaete worms is governed by both feeding ecology and mercury bioavailability in coastal mudflats

Polychaete worms are abundant in many mudflats but their importance to coastal food web Hg biomagnification is not known. We sampled sediments and polychaete worms from mudflats in the Bay of Fundy to investigate the bioaccumulation of mercury (Hg) and methylmercury (MeHg) in the coastal invertebrate food web. Hg concentrations in the sediments were low (<20 μg kg−1). Labile Hg (methanol/KOH sediment extraction) in surface sediments (0–1 cm) was positively correlated with Hg bioaccumulation by surface sediment-ingesting polychaetes but, surprisingly, there was a negative correlation between δ15N (i.e. trophic level) and THg bioaccumulation factors in polychaete worms. Worms feeding on deeper sediments contained the greatest MeHg concentrations (69.6 μg kg−1). Polychaetes are an important vector for Hg biomagnification to the coastal avian food web. This research demonstrates that feeding depth and method of feeding are more important than trophic position or sediment Hg concentrations for predicting Hg bioaccumulation.

Coordinated weather balloon solar radiation measurements during a solar eclipse

Solar eclipses provide a rapidly changing solar radiation environment. These changes can be studied using simple photodiode sensors, if the radiation reaching the sensors is unaffected by cloud.Transporting the sensors aloft using standard meteorological instrument packages modified to carry extra sensors, provides one promising but hitherto unexploited possibility for making solar eclipse radiation measurements. For the 20th March 2015 solar eclipse, a coordinated campaign of balloon-carried solar radiation measurements was undertaken from Reading (51.44N, 0.94W), Lerwick (60.15N, 1.13W) and Reykjavik (64.13N, 21.90W), straddling the path of the eclipse.The balloons reached sufficient altitude at the eclipse time for eclipse-induced variations in solar radiation and solar limb darkening to be measured above cloud. Because the sensor platforms were free to swing, techniques have been evaluated to correct the measurements for their changing orientation. In the swing-averaged technique, the mean value across a set of swings was used to approximate the radiation falling on a horizontal surface; in the swing-maximum technique, the direct beam was estimated by assuming the sensing surface becomes normal to the solar beam direction at a maximum swing. Both approaches, essentially independent,give values that agree with theoretical expectations for the eclipse-induced radiation changes.

Estoque e qualidade da matéria orgânica do solo em função de sistemas de culturas e adubação nitrogenada no sistema plantio direto

A matéria orgânica determina os principais atributos que definem a qualidade do solo, sendo diretamente afetada pelas práticas de manejo. Esta pesquisa objetivou ampliar o conhecimento referente às influências do sistema de culturas e da adubação nitrogenada no estoque e na qualidade da matéria orgânica do solo no sistema plantio direto. O estudo foi desenvolvido a partir de um experimento de longa duração (17 anos), constituído por dez sistemas de culturas em plantio direto e estabelecido num Argissolo inicialmente degradado pelo cultivo. Utilizou-se os sistemas aveia/milho, lablab+milho e guandu+milho, com e sem adubação nitrogenada (N). O tratamento solo descoberto, da área experimental, e o campo nativo também foram incluídos no estudo. Dez camadas de solo foram amostradas até a profundidade de 107,5 cm e três destas (0-2,5; 2,5-7,5 e 37,5-47,5 cm) foram fracionadas fisicamente nas frações matéria orgânica particulada (MOP), silte e argila. Os estoques de C e N foram avaliados em cada camada e também nas frações físicas. A qualidade da matéria orgânica foi investigada por espectroscopia de RMN-13C e de RMN- 15N (estado sólido), hidrólise ácida e pirólise analítica O estoque de C da camada de 0-107,5 cm reduziu, durante o período experimental, em média 5,8 Mg C ha-1 nos sistemas solo descoberto e aveia/milho (sem N), permaneceu constante no sistema aveia/milho (com N) e aumentou em média 20,6 Mg C ha-1 nos sistemas lablab+milho e guandu+milho (sem N e com N). O mesmo ocorreu nos estoques de C e N das frações físicas, sendo que as alterações foram relativamente maiores na MOP. A fração argila apresentou uma capacidade finita de estocar C (47,7 g C kg-1 de argila). Os sistemas de culturas afetaram a qualidade da MOP, porém não da matéria orgânica das frações silte e argila. A adubação nitrogenada tendeu a aumentar a proporção de C alquil na fração argila. A principal forma de carbono da matéria orgânica do solo foi C O-alquil. Material carbonizado (carvão) foi encontrado na fração silte da camada de 37,5-47,5 cm. O manejo do solo atua principalmente sobre os estoques e não tanto sobre a qualidade da matéria orgânica do solo.

Influência das espécies ativas na absorção de intersticiais durante a carbonitretação a plasma do TI

Physical-chemical properties of Ti are sensible to the presence of interstitial elements. In the case of thermochemical treatments plasma assisted, the influence of different active species is not still understood. In order to contribute for such knowledge, this work purposes a study of the role played by the active species atmosphere into the Ar N2 CH4 carbonitriding plasma. It was carried out a plasma diagnostic by OES (Optical Emission Spectroscopy) in the z Ar y N2 x CH4 plasma mixture, in which z, y and x indexes represent gas flow variable from 0 to 4 sccm (cm3/min). The diagnostic presents abrupt variations of emission intensities associated to the species in determined conditions. Therefore, they were selected in order to carry out the chemical treatment and then to investigate their influences. Commercial pure Ti disks were submitted to plasma carbonitriding process using pre-established conditions from the OES measurements while some parameters such as pressure and temperature were maintained constant. The concentration profiles of interstitial elements (C and N atoms) were determined by Resonant Nuclear Reaction Analysis (NRA) resulting in a depth profile plots. The reactions used were 15N(ρ,αγ)12C and 12C(α,α)12C. GIXRD (Grazing Incidence X-Ray Diffraction) analysis was used in order to identify the presence of phases on the surface. Micro-Raman spectroscopy was used in order to qualitatively study the carbon into the TiCxN1 structure. It has been verified which the density species effectively influences more the diffusion of particles into the Ti lattice and characteristics of the layer formed than the gas concentration. High intensity of N2 + (391,4 nm) and CH (387,1 nm) species promotes more diffusion of C and N. It was observed that Hα (656,3 nm) species acts like a catalyzer allowing a deeper diffusion of nitrogen and carbon into the titanium lattice.

Efeitos de fontes de nitrogênio e de modos de aplicação do adubo fosfatado (32P) sobre a porcentagem de utilização do fósforo do superfosfato simples pelo trigo (Triticum aestivum L.)

No programa de pesquisa a respeito da adubação da cultura de trigo, utilizando isotopos (32P e 15N), o plano do ano 1971/1972 tinha, entre outros objetivos, a finalidade de determinar a porcentagem de aproveitamento do fósforo pelo trigo, em função do modo de aplicação e interação com fontes de nitrogênio. Oexperimento foi instalado em solo pertencente a ordem Ultisol e grande grupo Podzólico Vermelho-Amarelo (Pelotas-RS), contendo 9 ppm de P. Os adubos nitrogenados: sulfato de amônio, uréia e nitrato de amônio, na dose de 120 kg/ha, foram aplicados metade no plantio (ao lado e abaixo das sementes) e metade em cobertura, na fase do perfilhamento. O fósforo radioativo, na forma de superfosfato simples, foi aplicado na dose de 30 kg P2O5/ha de duas maneiras: junto com as sementes ou junto com o adubo nitrogenado aplicado no plantio. Amostras de plantas de trigo foram coletadas entre perfilhamento e espigamento e determinaram-se os pesos de matéria verde e seca, as porcentagens de P total e de P proveniente do fertilizante contidos na matéria seca e a porcentagem de utilização do fósforo aplicado (% UP). Pode-se concluir que a aplicação de nitrogênio é necessário para se obter maio res % UP, mas não há diferenças entre as fontes. Os modos de aplicação do fósforo não diferiram entre si quanto a % UP.

Recuperação do nitrogênio da mistura de uréia e sulfato de amônio por plantas do milho

A mistura de uréia com fertilizantes de características ácidas aplicada ao solo pode aumentar a concentração de íons H+ próximos do grânulo e promover a redução da perda de N por volatilização. O experimento foi desenvolvido em vasos com 15 kg de Latossolo Vermelho textura média, sob túnel plástico, em Botucatu (SP), nos quais foram crescidas plantas de milho (duas plantas por vaso) até o pendoamento (66 dias após a emergência - DAE). Como tratamentos foi realizada a adubação (100 mg dm-3 N), no estádio de cinco folhas (30 DAE) utilizando os seguintes fertilizantes ou misturas físicas: (1) uréia (UR), enriquecida com 15N; (2) sulfato de amônio (SA), enriquecido com 15N; (3) sulfnitro (80% de N-UR e 20% de N-SA no mesmo grânulo); (4) mistura de UR (80% N) e SA (20% N e enriquecido com 15N); (5) mistura de UR (50% N) e SA (50% N), enriquecidos com 15N; (6) mistura de UR (50% N) e SA (50% N e enriquecido com 15N), (7) mistura de UR (50% N) e SA (50% N), enriquecido com 15N, diluídos em água (solução contendo 3% de N) e mais um tratamento que não recebeu N. A mistura de UR e SA não promoveu aumento na recuperação do N da uréia na planta de milho. do total de 15N-fertilizante aplicado, aproximadamente, 67% foram recuperados pela planta de milho (29% nas folhas, 25% no caule e 13% nas raízes) e 6% no solo, com uma perda estimada de 27%. O 15N da uréia foi recuperado em menor quantidade no caule em relação ao N do sulfato de amônio.

Analysis of the molecular association of rifampicin with hydroxypropyl-²-cyclodextrin

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Absorção de nitrogênio nativo do solo pelo milho sob plantio direto em sucessão a plantas de cobertura

O não revolvimento do solo com cultivo de adubos verdes na entressafra e o manejo da adubação nitrogenada alteram, geralmente, a dinâmica e a recuperação do N no sistema solo-planta. O objetivo deste trabalho foi avaliar a quantidade de N nativo do solo absorvido pelo milho em plantio direto, sob diferentes doses de N, em sucessão à crotalária (Crotalaria juncea), ao milheto (Pennisetum americanum) e à vegetação espontânea (pousio) em um Latossolo Vermelho no Cerrado. O estudo foi desenvolvido na fazenda experimental da Faculdade de Engenharia de Ilha Solteira-UNESP, Selvíria-MS, nos anos agrícolas 2001/02 e 2002/03. O delineamento experimental foi o de blocos casualizados com 15 tratamentos e quatro repetições, dispostos em esquema fatorial, 5 x 3, sendo cinco doses de N, na forma de uréia (0, 30, 80, 130 e 180 kg ha-1) e três sistemas de cobertura do solo (crotalária, milheto e o solo em pousio na entressafra). A quantidade de N nativo do solo absorvida pelo milho foi calculada pela diferença entre total de N acumulado na planta de milho, na época da maturação fisiológica, e o somatório do N proveniente da uréia, do milheto ou da crotalária determinados pelo método isotópico com 15N. O solo forneceu a maior quantidade de N para o milho, comparada à do fertilizante inorgânico conjuntamente à dos adubos verdes. As doses de N e os sistemas de cobertura do solo influenciaram significativamente a absorção de N nativo do solo pelo milho.