Spatial and temporal patterns in the demersal fish community on the shelf and upper slope regions of the Gulf of Alaska

We analyzed data from National Marine Fisheries Service bottom trawl surveys carried out triennially from 1984 to 1996 in the Gulf of Alaska (GOA). The continental shelf and upper slope (0–500 m) of the GOA support a rich demersal fish fauna dominated by arrowtooth flounder (Atheresthes stomias), walleye pollock (Theragra chalcogramma), Pacific cod (Gadus macrocephalus), Pacific halibut (Hippoglossus stenolepis), and Pacific Ocean perch (Sebastes alutus). Average catch per unit of effort (CPUE) of all groundfish species combined increased with depth and had a significant peak near the shelf break at 150–200 m. Species richness and diversity had significant peaks at 200–300 m. The western GOA was characterized by higher CPUEs and lower species richness and diversity than the eastern GOA. Highest CPUEs were observed in Shelikof Strait, along the shelf break and upper slope south of Kodiak Island, and on the banks and in the gullies northeast of Kodiak Island. Significant differences in total CPUE among surveys suggest a 40% increase in total groundfish biomass between 1984 and 1996. A multivariate analysis of the CPUE of 72 groundfish taxa revealed strong gradients in species composition with depth and from east to west, and a weak but significant trend in species composition over time. The trend over time was associated with increases in the frequency of occurrence and CPUE of at least eight taxa, including skates (Rajidae), capelin (Mallotus villosus), three flatfish species, and Pacific Ocean perch, and decreases in frequency of occurrence and CPUE of several sculpin (Myoxocephalus spp.) species. Results are discussed in terms of spatial and temporal patterns in productivity and in the context of their ecological and management implications.

Distribution and co-occurrence of rockfishes (family: Sebastidae) over trawlable shelf and slope habitats of California and southern Oregon

The rockfishes of the sebastid genus Sebastes are a very important fishery resource off the coasts of California and southern Oregon. How-ever, many of the 54 managed stocks of west coast rockfish have recently reached historically low population levels, leading fishery managers to re-examine current management practices. Management of rockfish stocks as multispecies aggregates, as opposed to independent stocks within the ground-fish fishery, can be more desirable when nontargeted bycatch, discard, and management complexity are considered. Rockfish assemblage structure and species co-occurrences were determined by using data from the Alaska Fisheries Science Center triennial continental shelf bottom trawl survey. The weight of rockfish species in trawl catches was expressed as a catch-per-unit-of-effort (CPUE) statistic, from which species spatial distributions, overlaps, diversity, and richness were analyzed. Multidimensional scaling of transformed CPUE data was employed in indirect gradient and multivariate partitioning analyses to quantify assemblage relationships. Results indicated that rockfish distributions closely match the bathymetry of coastal waters. Indirect gradient analysis suggested that depth and latitude are the principal factors in structuring the spatial distributions of rockfish on trawlable habitat. In addition, four assemblages were identified through the joint evaluation of species’ distributions and multivariate partitioning analyses: 1) deep-water slope; 2) northern shelf; 3) southern shelf; and 4) nearshore. The slope, shelf, and near-shore groups are found in depth ranges of 200–500 m, 100–250 m, and 50–150 m, respectively. The division of northern and southern shelf assemblages occurs over a broad area between Cape Mendocino and Monterey Canyon. The results of this analysis are likely to have direct application in the management of rockfish stocks off the coasts of southern Oregon and California.

Seismic performance of geomembrane placed on a slope in a MSW landfill cell -A centrifuge study

Geomembranes are one of the most commonly used geosynthetics in landfill liner systems. They retain the leachate produced by the waste and prevent leakage. Geomembranes may experience harsh environmental conditions such as extreme temperatures or earthquake loading. Earthquake loading can be an extreme loading case for landfills located in seismic regions. This study, based on dynamic centrifuge testing, investigates the effects of simulated earthquake loading on the tension experienced bythe geomembrane on a landfill slope. The landfill modeled in the dynamic centrifuge test was a municipal solid waste (MSW) landfill cell with a single geomembrane-clay liner system (45° side slope and 10 m slope length). The paper shows that moderate earthquake loading (base acceleration between 0.1g to 0.2g) can result in transient increases of around 20% in geomembrane tension, with permanent tension increases of around 5%.

Defining the orientation of the tandem fusions that occurred during the evolution of Indian muntjac chromosomes by BAC mapping

The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n=6 in the female and 7 in the male, the karyotypic evolution of which through extensive tandem fusions and several centric fusions has been well-documented by recent molecular cytogenetic studies. In an attempt to define the fusion orientations of conserved chromosomal segments and the molecular mechanisms underlying the tandem fusions, we have constructed a highly redundant (more than six times of whole genome coverage) bacterial artificial chromosome (BAC) library of Indian muntjac. The BAC library contains 124,800 clones with no chromosome bias and has an average insert DNA size of 120 kb. A total of 223 clones have been mapped by fluorescent in situ hybridization onto the chromosomes of both Indian muntjac and Chinese muntjac and a high-resolution comparative map has been established. Our mapping results demonstrate that all tandem fusions that occurred during the evolution of Indian muntjac karyotype from the acrocentric 2n=70 hypothetical ancestral karyotype are centromere-telomere (head-tail) fusions.