AVALIAÇÃO DE DUAS METODOLOGIAS DE EXPANSÃO AO FORNO do POLVILHO AZEDO

Duas metodologias de expansão ao forno foram comparadas em 52 amostras de polvilho azedo de três Estados: Santa Catarina, Paraná e Minas Gerais. Os resultados obtidos permitiram visualizar uma proposta de classificação em nível nacional da expansão ao forno do polvilho azedo. No método instrumental foi usado o Farinógrafo Brabender para estabelecer uma massa de consistência padrão de 60UF e no método prático, a consistência semelhante da massa foi determinada de forma empírica. Com a metodologia instrumental obteve-se biscoitos com expansão superior à metodologia prática, mas maior coeficiente de variação, o que pode ser devido a maior sensibilidade do método. Ambas as metodologias poderão ter aplicação, dependendo da finalidade. A metodologia instrumental, pelo custo do equipamento, é mais adequada para laboratórios, desde que se utilize maior número de repetições, para reduzir o coeficiente de variação. Ambas as metodologias ensaiadas permitiram classificar as amostras com o mesmo perfil de desempenho. As amostras provenientes do Estado do Paraná apresentaram as maiores expansões ao forno quando comparados com os outros Estados. Pela metodologia prática a expansão média das amostras do Estado do Paraná foi de 14,1mL/g. As amostras do Estado de Minas Gerais apresentaram as menores expansões ao forno, em média de 13,3mL/g. Foi proposta neste artigo uma classificação para avaliar a qualidade do polvilho azedo, complementando a legislação brasileira que não estabelece este índice para o produto. Quanto à propriedade de expansão ao forno, as amostras avaliadas podem ser divididas em 3 tipos A, B e C. No tipo A (10%) amostras de polvilhos azedo são classificadas como qualidade extra, no tipo B (80%) os polvilhos são de média qualidade e o tipo C (10%) de qualidade inferior. Os resultados obtidos pela metodologia prática permitiram estabelecer como tipo A o polvilho azedo que apresenta um índice de expansão ao forno superior a 16,0mL/g, o tipo B o polvilho com índice de expansão entre 12,0mL/g a 16,0mL/g e o tipo C o polvilho com índice de expansão menor que 12,0mL/g.

Ocorrência e caracterização molecular de Cryptosporidium em cordeiros

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ultrastructure of spermatogonia and primary spermatocytes of C57BL6J mice

The three types of spermatogonia were confirmed. Type A spermatogonia have a large nucleus and loose chromatin and are poor in endoplasmic reticulum. The second type, B spermatogonia, have rounded and smaller nuclei filled with more electron-dense nucleoplasmic material. The endoplasmic reticulum has the aspect of round or elongated cisterns that are free in the cytoplasm or close to the basement membrane. In contrast, intermediate spermatogonia present chromatin material with intermediate condensation compared with the two previous cell types. Primary spermatocytes are characterized by the presence of intercellular bridges and a synaptonemal complex. In the late pachytene stages, the synaptonemal complex was found to be enveloped by chromatin material.

Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Bioacoustic analysis of advertisement call in Hyla nana and Hyla sanborni (Anura, Hylidae) in Botucatu, São Paulo, Brazil

As características relacionadas à comunicação sonora em duas espécies aparentadas, Hyla nana e H. sanborni, pertencentes ao grupo nana de espécies, foram estudadas, entre agosto de 1997 e junho de 1999, em duas poças permanentes de grande porte em área aberta na região de Botucatu, Estado de São Paulo. Foram obtidas gravações de 131 exemplares, 71 indivíduos de H. nana e 58 de H. sanborni, durante início de atividade de vocalização e atividade de vocalização em coro. Houve diferença nos ritmos de emissão de notas dos cantos entre o início das atividades e durante os coros. O canto de anúncio das espécies consiste na emissão de séries consecutivas de notas simples, pulsadas, com taxa de repetição rápida. Hyla nana e H. sanborni apresentam dois tipos de notas em seu canto de anúncio, denominados aqui como tipos A e B. Notas do tipo A, introdutórias, apresentam maior duração e número de pulsos, e suas emissões foram mais freqüentes durante o início das atividades de vocalização. As notas introdutórias são as primeiras da série emitida em atividade de coro. As notas do tipo B, secundárias, são curtas e com menor número de pulsos, sendo emitidas durante as vocalizações em coro. Os dois tipos de notas encontrados diferem significativamente em sua estrutura temporal. As duas espécies apresentaram segregação acústica tanto na estrutura espectral como na temporal de seus cantos de anúncio.

CARACTERÍSTICAS DE QUALIDADE do CONTRAFILÉ (m. L. dorsi) DE TOUROS JOVENS da RAÇA NELORE

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeito do tamanho dos grânulos nas características estruturais e físico-químicas do amido de trigo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fezes de galinhas poedeiras na alimentação de ruminantes análises microbiológicas

The objectives of the present study were: to evaluate the variation in total number of microorganisms in laying hen fecal samples and to identify the pathogenic microorganisms possibly present such as Staphylococcus aureus, Salmonella sp., Clostridium botulinum, C chauvoei, Campylobacter sp., Escherichia coli, and Corynebacterium sp. The experiment was conducted at the School of Agrarian and Veterinarian Sciences of Jaboticabal, - UNESP. Fecal samples were collected from the aviary. A fully randomized experimental design was used with 7 treatments and 4 replications, for a total of 28 samples. A decrease in total number of microorganisms was detected when treatment T0 (zero days of storage) was compared with treatment T6 (42 days of storage), with oscillations in numbers being observed during this period of time. Pathogenic bacteria such as Clostridium noyvi, C perfringens, C sordelli, C septicum and C noyvi type B were detected, as well as non-pathogenic bacteria such as Bacillus sp. and Staphylococcus epidermidis.

THE FAMILY COMPSOPOGONACEAE (RHODOPHYTA) IN BRAZIL

A taxonomic revision of the family Compsopogonaceae in Brazil was carried out, resulting in the recognition of a single genus (Compsopogon) with two species (C. coeruleus and C. leptoclados). For both species the following information was provided: a) nomenclatural type; b) type locality; c) description (with illustrations); d) diagnostic features; e) world distribution; f) distribution in Brazil (with map); g) habitat, associated species and seasonality; h) remarks, including discussion of taxonomic and/or nomenclatural problems and comparison with previous reports based on Brazilian and foreign material. Currently accepted diagnostic criteria for genera and species within Compsopogonaceae are critically analysed. The circumscription of Compsopogon and Compsopogonopsis are evaluated and the single genus Compsopogon is recognized in the family.

Deletion of Epstein-Barr virus latent membrane protein 1 gene in United States and Brazilian Hodgkin's disease and reactive lymphoid tissue: High frequency of a 30-bp deletion

A 30-basepair (bp) deletion in the Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) gene has been reported in nasopharyngeal carcinoma and EBV-associated malignant lymphomas. Prior studies have found the deletion in about 10% to 28% of cases of Hodgkin's disease (HD), particularly in cases with aggressive histology. We studied the prevalence of 30-bp LMP1 gene deletion in EBV-positive HD in the United States (US) (12 cases) and Brazil (26 cases) with comparison to reactive lymphoid tissues (21 cases) and HD without EBV-positive Reed-Sternberg cells (15 cases). We studied the status of the LMP1 gene by Southern blot hybridization of polymerase chain reaction (PCR) products obtained after amplification with primers spanning the site of the deletion. We also performed EBV typing, EBER1 in situ hybridization, and LMP1 protein immunohistochemistry. EBV was detected in 12/26 (46%) cases of HD from the US and 26/27 (96%) cases of Brazilian HD. The 30-bp LMP1 gene deletion was observed in 4/12 (33%) cases of EBV-positive HD from US, and 12/26 (46%) cases of Brazilian EBV-positive HD, including 3 cases of type B EBV, as compared with 12/21 (57%) reactive lymphoid tissues and 9/15 (60%) cases of EBV-negative HD. US and Brazilian HD showed a higher prevalence of the 30-bp LMP1 gene deletion, compared with studies of others. The unexpected finding of high incidence of 30-bp deletion in LMP1 gene in reactive lymphoid tissue and HD without EBV-positive Reed-Sternberg cells suggests that this deletion may not be relevant to HD pathogenesis in most cases. Copyright (C) 1997 by W.B. Saunders Company.

Prevalence of different types of accessory canals in the furcation area of third molars

Background: Although there are several studies that show the prevalence and diameter of accessory root canals in the furcation area, there is a scarceness of studies that observe the trajectory and different types of cavo-interradicular canals. The aim of this study was to verify the prevalence of the different morphologic types of accessory canals in the furcation region in an attempt to show their trajectories.Methods: Forty submerged mandibular third molars were used, which were extracted and decalcified so that the microtomy procedure in the mesio-distal axial plane could be performed, obtaining semiserial sections with thicknesses of 5 mu m. The sections were stained with hematoxylin and eosin and observed under optical microscopy.Results: All of the morphologic types were found, whereas the proper accessory canals, type-A canals, were present in 10% of the specimens. The most prevalent canals were the sealed ones (type D), with a prevalence of 87.5%, followed by the blind ones (type B), with a prevalence of 75%. The loop accessory canals (type C), observed in only 5% of the teeth, were the least prevalent ones.Conclusions: Different morphologic types of accessory canals were found in the furcation area of submerged mandibular molars. The histologic method was effective to show the canal trajectories.

Evaluation of survival of Staphylococcus aureus and Clostridium botulinum in charqui meats

Charqui meats were prepared in laboratory conditions in order to carry out experiments to observe the possibility of development of enterotoxigenic Staphylococcus aureus and Clostridium bottilinum proteolytic type B spores and their toxins. Results demonstrated that the harsh processing conditions, high salt concentration, relative high temperature, a, values, inhibited the growth of both bacteria. Under our experimental conditions, S. aureus would survive throughout the sequence of salting steps i.e. brine followed by rock salting and the sunshine drying step. However, at final a(w) value of 0.70-0.75 would create conditions to inhibit its development. The other experiment revealed that C. botulinum spores germination also was impaired because of these low a(w) values. Under these conditions, charqui meats revealed to be safe products in relation to toxins from both enterotoxigenic S. aureus and C. botulinum. (C) 2003 Elsevier B.V. Ltd. All rights reserved.

Jarosite pseudomorph formation from arsenopyrite oxidation using Acidithiobacillus ferrooxidans

In this work, the oxidizing action of a native strain type A. ferrooxidans on a sulphide containing a predominance of arsenopyrite and pyrite has been evaluated. Incubation of the A. ferrooxidans strain in flasks containing 200 mL of T&K medium with the ore (particle size of 106 mu m) at pulp density 8% (w/v) at 35 degrees C on a rotary shaker at 200 rpm resulted in preferential oxidation of the arsenopyrite and the mobilization of 88% of the arsenic in 25 days. Mineralogical characterization of the residue after biooxidation was carried out with FTIR. XRD and SEM/XEDS techniques. An in situ oxidation of the arsenopyrite is suggested on the basis of the frequent appearance of jarosite pseudomorph replacing arsenopyrite, in which the transformations Fe(2+) -> Fe(3+), S(-2) -> S(+6) and As(-1) -> As(+3) -> As(+5) occur for the most part without formation of soluble intermediates, resulting in a type of jarosite that typically contains high concentrations of arsenic (type A-jarosite). However, during pyrite oxidation, dissolution of the constituent Fe and S predominates, which is evidenced by corrosion of pyrite particles with formation of pits, generating a type of jarosite with high quantities of K (type B-jarosite). Lastly, a third type of jarosite (type C-jarosite) also precipitated forming a thin film that covered the grains of pyrite principally. (C) 2010 Elsevier B.V. All rights reserved.

Morphological Characterization of the Testicular Cells and Seminiferous Epithelium Cycle in Six Species of Neotropical Bats

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Preparação e caracterização inicial de complexo de inclusão entre nitrofurazona e 2-hidroxipropil-β-ciclodextrina

Nitrofurazone (NF), 5-nitro-2-furaldehyde semicarbazone, a broad-spectrum antibiotic, has reported toxic effects and low solubility in water. It would be of great interest to form inclusion complexes between NF and a cyclodextrin, to develop more effective and safer antibiotic formulations. This paper focuses on the preparation of inclusion complexes of NF with 2-hydroxypropyl-β- cyclodextrin (HP-β-CD) and their initial characterization by evaluating rates of complex formation, photostability, solubility isotherms, release rate profiles, stoichiometry of the complexes and their morphology, as revealed by scanning electron microscopy. The kinetic tests of complex formation revealed that 17,3 h is enough for stabilization of the NF-cyclodextrin complex. The solubility isotherm studies showed that the isotherm changes from type A to type B, as a function of temperature. The photostability experiments showed that the insertion of the NF in the HP-β-CD cavity protects the drug from photodecomposition. The release kinetic tests showed that the profile of NF release from the complex is altered by the presence of HP-β-CD in the medium. A Job's plot indicated that the stoichiometry of the complex was 1:1 NF:HP-β-CD. The scanning electron micrographs showed changes in the crystal structure of NF in the complex. This study focused on the physicochemical properties of drug-delivery formulations that could potentially be developed into a novel type of therapy with NF.