Platelets roll on stimulated endothelium in vivo: an interaction mediated by endothelial P-selectin.

P-selectin, found in storage granules of platelets and endothelial cells, can be rapidly expressed upon stimulation. Mice lacking this membrane receptor exhibit a severe impairment of leukocyte rolling. We observed that, in addition to leukocytes, platelets were rolling in mesenteric venules of wild-type mice. To investigate the role of P-selectin in this process, resting or activated platelets from wild-type or P-selectin-deficient mice were fluorescently labeled and transfused into recipients of either genotype. Platelet-endothelial interactions were monitored by intravital microscopy. We observed rolling of either wild-type or P-selectin-deficient resting platelets on wild-type endothelium. Endothelial stimulation with the calcium ionophore A23187 increased the number of platelets rolling 4-fold. Activated P-selectin-deficient platelets behaved similarly, whereas activated wild-type platelets bound to leukocytes and were seen rolling together. Platelets of either genotype, resting or activated, interacted minimally with mutant endothelium even after A23187 treatment. The velocity of platelet rolling was 6- to 9-fold greater than that of leukocytes. Our results demonstrate that (i) platelets roll on endothelium in vivo, (ii) this interaction requires endothelial but not platelet P-selectin, and (iii) platelet rolling appears to be independent of platelet activation, indicating constitutive expression of a P-selectin ligand(s) on platelets. We have therefore observed an interesting parallel between platelets and leukocytes in that both of these blood cell types roll on stimulated vessel wall and that this process is dependent on the expression of endothelial P-selectin.

Acceleration and Deflection Analysis for Class C Edge Protection Systems in Construction Work

The requirements for edge protection systems on most sloped work surfaces (class C, according to EN 13374-2013 code) in construction works are studied in this paper. Maximum deceleration suffered by a falling body and maximum deflection of the protection system were analyzed through finite-element models and confirmed through full-scale experiments. The aim of this work is to determine which value for deflection system entails a safe deceleration for the human body. This value is compared with the requirements given by the current version of EN 13374-2013. An additional series of experiments were done to determine the acceleration linked to minimum deflection required by code (200 mm) during the retention process. According to the obtained results, a modification of this value is recommended. Additionally, a simple design formula for this falling protection system is proposed as a quick tool for the initial steps of design.

First Lady Hillary Clinton at Commencement, May 1, 1993, roll 11920-5, frame #9

[contact print, slide, and color neg. frame #9, roll 11920-5]