Conditional switching of vascular endothelial growth factor (VEGF) expression in tumors: Induction of endothelial cell shedding and regression of hemangioblastoma-like vessels by VEGF withdrawal

We have recently shown that VEGF functions as a survival factor for newly formed vessels during developmental neovascularization, but is not required for maintenance of mature vessels. Reasoning that expanding tumors contain a significant fraction of newly formed and remodeling vessels, we examined whether abrupt withdrawal of VEGF will result in regression of preformed tumor vessels. Using a tetracycline-regulated VEGF expression system in xenografted C6 glioma cells, we showed that shutting off VEGF production leads to detachment of endothelial cells from the walls of preformed vessels and their subsequent death by apoptosis. Vascular collapse then leads to hemorrhages and extensive tumor necrosis. These results suggest that enforced withdrawal of vascular survival factors can be applied to target preformed tumor vasculature in established tumors. The system was also used to examine phenotypes resulting from over-expression of VEGF. When expression of the transfected VEGF cDNA was continuously “on,” tumors became hyper-vascularized with abnormally large vessels, presumably arising from excessive fusions. Tumors were significantly less necrotic, suggesting that necrosis in these tumors is the result of insufficient angiogenesis.

Life-span, T-cell responses, and incidence of lymphomas in congenic mice.

Survival, T-cell functions, and postmortem histopathology were studied in H-2 congenic strains of mice bearing H-2b, H-2k, and H-2d haplotypes. Males lived longer than females in all homozygous and heterozygous combinations except for H-2d homozygotes, which showed no differences between males and females. Association of heterozygosity with longer survival was observed only with H-2b/H-2b and H-2b/H-2d mice. Analysis using classification and regression trees (CART) showed that both males and females of H-2b homozygous and H-2k/H-2b mice had the shortest life-span of the strains studied. In histopathological analyses, lymphomas were noted to be more frequent in females, while hemangiosarcomas and hepatomas were more frequent in males. Lymphomas appeared earlier than hepatomas or hemangiosarcomas. The incidence of lymphomas was associated with the H-2 haplotype--e.g., H-2b homozygous mice had more lymphomas than did mice of the H-2d haplotype. More vigorous T-cell function was maintained with age (27 months) in H-2d, H-2b/H-2d, and H-2d/H-2k mice as compared with H-2b, H-2k, and H-2b/H-2k mice, which showed a decline of T-cell responses with age.

Way of scoring by Spanish First Division volleyball teams in relation to winning/losing, home/away, final classification, and type of confrontation

The aim of this study was to assess the way volleyball teams score with regard to: whether or not they won the game, whether they were the home or away team, the level of the opposing teams, and the type of confrontation. The sample was composed of 118,083 plays from 794 men’s volleyball matches and 125,751 plays from 719 women’s matches of Spain’s first division clubs (from the 2002-2003 season to the 2006-2007 season). The variables studied were: the way points were obtained in each play, being the home or away team, the level of the teams, the result of the match, and the type of confrontation between the teams with regard to their level. The results demonstrate that for both men’s and women’s teams, the majority of the points were obtained in attack and by opponent errors. Differences were found with regard to the way points were obtained when winning or losing the match was taken into account as well as when considering the level of the teams. This paper discusses the differences found with regard to whether the team is home or visiting and the type of confrontation.

Petrographical classification and mineral assemblage of basement clasts from the Quaternary and Miocene sections of sediment core CRP-1 (Table 1)

Petrographical and mineral chemistry data are described for the mist representative basement lithologies occurring as clasts (pebble grain-size class) from the CRP-1 drillhole. Most pebbles consits of either undeformed or foliated biotite with or without hornblende monzogranites. Other rock types include biotite with or without garnet syenogranitr, biotite-hornblende granodiorite, tonalite, monzogranitic porphyries, haplogranite, quartz-monzonite (restricted to the Quaternary section), Ca-silicate rocks and biotite amphibolite (restricted to the Miocene strata). The common and ubiquitous occurence of biotite with or without hornblende monzogranite pebbles, in both the Quaternary and Miocene sections, apparently mirrors the dominance of these rock types in the granitoid assemblages which are presently exposed in the upper Precambrian-lower Paleozoic basement of the south Victoria Land. The other CRP-1 pebble lithologies show petrographical features which consitently support a dominant supply from areas of the Transantarctic Mountains located to the west and south-west of the CRP-1 site, and they thus furthercorroborate a model of local provenance for the supply of basement clasts to the CRP-1 sedimentary strata.

OPTREG - an interactive computer program for optimization and regression. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

OPM's classification and qualification systems : a renewed emphasis, a changing perspective : a report to the President and the Congress of the United States /

Mode of access: Internet.

Landscape with Cottage and Big Tree

Rembrandt van Rijn; 5 3/64 in.x 1 ft. 19/32 in.; etching and pen on paper

Reconnaissance of Red Rock District, Cove Mesa, and Kinusta (tree) Mesa, Arizona /

Red Rock district, Cove mesa, and Kinusta mesa lie in southern Carrizo Mountain region of Apache County, Arizona, 25 miles south of the Utah State line. The Red Rock district lies along the Arizona-New Mexico line, and the two mesas are approximately 15 miles to the west (Plate I). All of these areas are within the boundaries of Navajo reservations in Arizona and New Mexico.

Farm appraisal : classification and valuation of farm land and buildings /

Includes bibliographies.

Farm appraisal : classification and valuation of farm land and buildings /

Includes bibliographies.