Determinação de resíduo do herbicida fluridone em peixe

O objetivo deste trabalho foi desenvolver um método de análise de resíduos do herbicida fluridone em peixes. O herbicida foi extraído do peixe (partes comestíveis), concentrado em colunas de florisil e então analisado em cromatógrafo líquido com detector de UV visível a 313 nm. Os coeficientes de correlação obtidos para linearidade do detector e do método foram de 0,99983 e 0,99987, respectivamente. Com o método descrito foi possível extrair, separar e identificar com eficiência o herbicida fluridone das amostras de peixe, o qual apresentou médias de percentagens de recuperação variando de 60 a 73%. O limite de detecção foi de 20 mg kg-1.

Efeito do resfriamento sobre a textura post-mortem da carne do peixe matrinxã Brycon cephalus

Os mecanismos que causam o amolecimento e a perda na textura post-mortem da carne de matrinxã foram determinados por meio das mudanças na microestrutura do músculo, imediatamente após a morte e depois de 12 horas de estocagem a -3°C. As observações na microestrutura, realizadas com microscópio eletrônico de transmissão, foram semelhantes aos resultados obtidos na força de ruptura do músculo medidos com o texturômetro. Os valores da força da ruptura foram menores para a carne após o resfriamento. Observou-se que as fibras do colágeno do tecido conectivo pericelular se desintegraram e que as do colágeno do tecido conectivo do miocommata conservaram sua arquitetura e integridade. Houve pouca degradação da linha Z. Isso sugere que o amolecimento post-mortem da carne de mantrinxã, durante a estocagem a -3°C, é causado pela degradação do tecido conectivo pericelular.

Consórcio suíno-peixe: aspectos ambientais e qualidade do pescado

Amostras de músculo de carpa comum (Cyprinus carpio) e de água de três viveiros fertilizados com dejetos de suínos e de um viveiro em que os peixes foram alimentados com ração comercial para peixes foram analisadas durante seis meses. O objetivo foi observar a presença de bactérias patogênicas, como Salmonella, Staphylococcus aureus e coliformes totais e fecais, em peixes comercializados in natura no município de Chapecó, Santa Catarina. Foram aferidos quinzenalmente, na água dos quatro viveiros, oxigênio dissolvido, pH, turbidez, alcalinidade total, dureza total, amônia, nitrato e ortofosfato, bem como a quantidade de coliformes totais e fecais. A análise microbiológica músculo dos peixes demonstrou ausência de Salmonella em todas as amostras. A contagem dos demais microrganismos manteve-se inferior ao limite máximo permitido pela legislação vigente, em todas as amostras. Portanto, os peixes analisados estavam aptos ao consumo humano, pois atenderam aos padrões recomendados pela Divisão Nacional de Vigilância Sanitária de Alimentos (DINAL). A comparação entre os viveiros fertilizados com dejetos de suínos e o viveiro fertilizado com ração comercial para peixes não apresentou diferença significativa, porém apresentou efeito significativo sobre oxigênio dissolvido, turbidez, alcalinidade total e amônia. A matéria orgânica foi responsável pelos maiores valores, com exceção da turbidez, que foi menor no viveiro fertilizado com ração comercial para peixes. Entretanto, após análise de todos os parâmetros físico-químicos e microbiológicos da água dos viveiros, pode-se constatar que todos os viveiros se enquadraram nas classes II ou III da classificação de águas interiores estabelecida pelo CONAMA.

Estimativa da concentração espermática do sêmen de peixe pelo método de espermatócrito

Um experimento foi realizado com o objetivo de estimar a concentração espermática das espécies dourado (Salminus brasiliensis), curimba (Prochilodus lineatus), jundiá (Rhamdia quelen), cascudo-preto (Rhinelepis aspera) e tilápia-do-nilo (Oreochromis niloticus) pelo método de espermatócrito. Utilizaram-se 19, 58, 51, 43 e 85 reprodutores de dourado, curimba, jundiá, cascudo-preto e tilápia-do-nilo, respectivamente. Com exceção da tilápia-do-nilo, os reprodutores foram submetidos ao processo de indução hormonal e posteriormente submetidos a coleta de sêmen. Foram comparadas as técnicas de mensuração da concentração espermática do sêmen por contagem em câmara hematimétrica de Neubauer e por espermatócrito. Os resultados obtidos foram submetidos à análise de regressão a 5% de probabilidade. As concentrações espermáticas mensuradas por ambas as técnicas apresentaram relação linear, para curimbas, jundiás e tilápias-do-nilo, com equações y = 6,6624 × 10(9) + 3,68553 × 10(8)x; y = 2,153 × 10(9) + 4,426 × 10(8)x e y = -9,0897 × 10(8) + 6,0167 × 10(8), respectivamente. O método de espermatócrito pode ser utilizado para estimar a concentração espermática do sêmen de curimbas, jundiás e tilápias-do-nilo.

Avaliação das Farinhas de Peixe e Pena, no Confinamento de Bezerros Leiteiros Desmamados, Através de Dietas Calculadas em Termos de Proteína Bruta ou de Proteína Metabolizável

The objectives of this work were to evaluate three protein sources - soybean meal, fish meal and feather meal - and two methods of calculation of rations - the calculated in terms of crude protein (CP), according to recommendations of NRC (1988); and the proposed by the AFRC (1993), and calculated in terms of metabolizable protein (MP) - through the performance of calves. The animals were confined and submitted to five diets, that varied only protein sources in its composition, thus discriminated: SM- Soybean Meal, FsM- Fish Meal, FeM- Feather Meal, FsMU- Fish Meal and Urea, and FeMU Feather Meal and Urea. A complete diet was supplied, composed of 40% of sorghum silage and 60% of concentrate. All treatments possessed about of 2.56 Mcal/kg DM of metabolizable energy, being the treatments SM, FsM and FeM calculated by the system of CP, with 18% of CP, and the treatments FsMU and FeMU, calculated by the system of MP, with 16.3% of CP and a same amount of metabolizable protein than the treatment SM, with 112.0 grams of MP/day. Individually, the treatments did not present significant differences (P > 0.05) in the final alive weight, in daily gain weight and in dry matter intake, having differences only in the feed:gain ratio. The best feed:gain ratio happened in the treatment FsM and the worst in the treatment FeMU. It is concluded that the MP method provides similar results to the method of CP. The treatments with fish meal provide larger weight gains, smaller intake and better feed:gain ratio than the treatments with feather meal, staying the treatment soybean meal with intermediary daily gain.

Efeitos tóxicos de surfactantes fitossanitários para o peixe mato grosso (Hyphessobrycon eques)

Toxic effects of phytosanitary surfactants for jewel tetra (Hyphessobrycon eques). Surfactants are amphipatic molecules that reduce the surface tension of water and make up the inert components of pesticide formulations. Thus, the objectives of this study were: to estimate the lethal concentration (LC(I)50;96h); classify and evaluate water quality during testing of the following surfactants: Agral[registered trademark], Aterbane[registered trademark]BR, Ag-bem[registered trademark], Energic[registered trademark], Fixade[registered trademark] and Gotafix[registered trademark] for jewel tetra (Hyphessobrycon eques); and the signs of intoxication in the animals. For this, the fish were acclimated for ten days in the bioassay room. The animals were exposed to the surfactants in an entirely randomized design with three replications. The LC(I)(50;96h) of surfactant Agral[registered trademark] was 3.29 mg L-1; Aterbane[registered trademark] BR 8.21 mg L-1; Energic[registered trademark] 2.34 mg L-1; Gotafix[registered trademark] 4.37 mg L-1; Fixade[registered trademark] 3.38 mg L-1; and Ag-bem[registered trademark] 34.95 mg L-1. The variables of water quality were unchanged. The fish showed an increase in the opercular beating after exposure; 4 and 24 hours, loss of gasping ability; 48 hours, slow opercular beating; and 72 and 96 hours later, recovery. The surfactants Energic[registered trademark], Agral[registered trademark], Gotafix[registered trademark], Aterbane[registered trademark] BR and Fixade[registered trademark] can be classified as moderately toxics, and Ag-Bem[registered trademark] as slightly toxic for H. eques; this organism shows similar intoxication signs for all surfactants.

Efeito da extrusão termoplástica no teor de usina disponível da farinha desengordurada de grão-de-bico (Cicer arietinum, L.)

The aim of this research was to evaluate lysine availability of chickpea (Cicer arietinum, L.) flour submitted to thermoplastic extrusion at three feed moisture levels (13%, 18% and 27%). It was verified that extrusion treatments reduced available lysine by 58% and 55% at 13% and 18% feed moisture levels. The major lysine loss, 71%, was verified at 27% feed moisture level.

Aplicação do Índice Relaçáo Declividade-Extensão - RDE Na Bacia do Rio do Peixe (SP) para Detecção de Deformações Neotectô nicas

This paper presents the identification of tectonic deformation through the application of the stream-gradient index (slope x length) method in the Rio do Peixe hydrographic basin, located in western State of Sã o Paulo. The main principle used is that river valleys are the best tools for this type of analysis because of their fast adjustment to even the most gentle crustal deformations. This type of analysis for structural studies is extremely useful in the western region of the State of São Paulo, where deep chemical weathering results in a thick regolith and scarce outcrops. The stream length index is the ratio of the altimetric amplitude of each drainage and the natural logarithm of its length. The value obtained is plotted on maps at the median point of each drainage, allowing the drawings of lines with the same values (isodefs). Three anomalies (A, B and C) were identified along the Rio do Peixe valley representing uplift sectors indicated by the formation and distribution of Upper Quaternary deposits in terraces and modern fluvial plains and alluvial fans. In correlation with known tectonic data for this region it is possible to attribute the A and B anomalies to the Ribeirão Preto and Presidente Prudente crustal sutures, respectively. The smaller C anomaly needs further field investigation, but it could be associated with the Três Lagoas crustal suture. It must be emphasised that these sutures are inherited from the Precambrian Basement which had a strong influence on the Phanerozoic evolution in the whole sedimentary and volcanic stratigraphic pile of the Paraná Basin and on the dominant structural lineaments directions developed therein .The A, Band C anomalies are suggestive of renewed uplift as a result of neotectonic action in modern times.

O impacto da pesca do camarão-rosa Farfantepenaeus paulensis (Perez-Farfante) (Decapoda, Penaeidae) nas assembléias de peixes e siris do Parque Nacional da Lagoa do Peixe, Rio Grande do Sul, Brasil

This work evaluates the impact of the pink shrimp Farfantepenaeus paulensis (Perez-Farfante, 1967) fishery on fish and crab assemblages of Lagoa do Peixe National Park, Brazil. We observed that composition of catches is similar to shrimp fisheries using fyke-net at others estuaries of Rio Grande do Sul State: pink shrimp (53%), accessory catches (24%) and bycatch (23%). However, fishery composition showed distinct differences along the saline gradient of the main park's lagoon. Regarding only fish species, the most impacted species in this fishery were Brevoortia pectinata (Jenyns, 1842), Micropogonias furnieri (Desmarest, 1823), and Jenynsia multidentata (Jenyns, 1842). In others estuaries from Rio Grande do Sul, in contrast, the most impacted species were M. furnieri, Genidens barbus (Lacepède, 1803) and Genidens genidens (Cuvier, 1829). The potential impact of the pink shrimp fishery at Lagoa do Peixe National Park seemed to be weaker when compared to shrimp fisheries elsewhere. We believe that the decision to prohibit this fishery at the Lagoa do Peixe National Park should not be based on its potential damage to the fish and crab assemblages but based on the simple fact that Brazilian laws do not allow fisheries inside National Parks.

Detecção de prováveis deformações neotectônicas no Vale do Rio do Peixe, Região Ocidental Paulista, mediante aplicaçao de Índices RDE (Relac̈ão Declividade-Extensão) em segmentos de drenagem

Running water is one of the most important of all the physical processes which fashion the landscape, allowing gravity to operate along the valley floors. Besides this, the streams show a fast adjustment to the crustal deformations, even to the most gentle ones. This geologic behavior turns them a potential tool for neotectonic studies, specially the analysis of morphotnetric parameters associated with hydraulic gradient and discharge, this second factor being directly proportional to the extension of the streams. Both elements, gradient and stream length, can be combined in the SL index. The purpose of this paper is to show the RDE index application in the neotectonics analysis of the Rio do Peixe hydrographic basin and to compare the obtained values with the geologic basement incised by the streams. This basement encompasses Cretaceous sedimentary rocks of post-Serra Oeral Formation magmatism (Caiuá and Bauru groups) and Quaternary deposits that include chiefly recent alluvial plains and some Pleistocene terrace deposits. In the final part of this paper, an attempt is made in order to correlate the RDE results and the neotectonic framework admitted to this portion of the São Paulo State territory, as well as with field geologic, seismologic and paleoseismologic known elements. The results indicate the presence of two groups of anomalies: The first set corresponds to the Marília-Exaporã Plateau border, and the second one, located in the central portion of the hydrographic basin, is correlated to the Presidente Prudente seimogenic zone.

Metodologia para quantificação de leucóciotos totais em peixe Oreochromis Niloticus

Haematological investigation is an important part of disease diagnosis. The techniques used for mammals are generally applicable for fishes with slight modification. The presence of nucleated erythrocytes and thrombocytes in fish may cause some confusion in the identification of blood cells, mainly in total leukocytes count. This work evaluated two different methods (direct and indirect) of leukocytes counting. Specimens of tilapia were divided randomly into four groups of 48 fish each. Each group was further randomized into three replicate experiments of 16 fish per replicate in 40 L aquarium and maintained for 10 days. Counting of leukocytes in peripheral blood was determined in six fishes per group at the times: 0, 3, 7 and 10 days. The direct method in Neubauer chamber showed a large dispersion of data in regard to the average, the opposite was observed in indirect method determined in blood smears that showed more homogeneity among groups. Due to this factor the indirect method can be considered more accurate than the direct method for counting leukocytes in fishes.

O uso da silagem de subprodutos da filetagem de peixe na alimentação de suínos em crescimento - parâmetros séricos

The study was carried out to evaluate the inclusion of increasing levels (0, 3,0 and 6.0%) of fish silage (SSFP), in the ration. The parameters studied were urea, uric acid, total protein, triglycerides, total cholesterol and lipoproteins (LDL, HDL and VLDL). Eighteen female piglets Moura weighting and crossbred Duroc x Moura, were used with 24,0± 3,0 kg in average weight and 90 days of age and eighteen male pigs Moura and crossbred Duroc x Moura, with 31,5±5,0 kg in average weight and 70 days of age in a randomized design with 3 treatments. The results showed no effect to the addition up to 6,0% of SSPF in the swine diet neither for in growth, nor to the serum parameters, even if had been found alteratum on the urea plasmatic concentrations experiments. All the parameters was closed to the normal value recommended in the literature.

Ocorrência do peixe-pescador-branco, Lophiodes beroe Caruso, 1981 (Lophiiformes: Lophiidae), em águas brasileiras

One specimen of the lophiid white anglerfish, Lophiodes beroe was collected for the second time in Brazilian waters, which far extends the limit distribution of the species to southeastern region (25°00'08).

Efeito de hidrolisado de peixe sobre o crescimento micelial e controle de Cylindrocladium spathiphylli em espatifilo

Fish hydrolyzed (HP), poultry manure (CF), shrimp skin (CC), cattle manure (EB), sewage sludge (LE) and castorbean presscake (TM) were evaluated for their effect of aqueous extracts with and without autoclaving, on mycelial growth and conidial germination of Cylindrocladium spathiphylli. The effect of mixtures of residues with potting mixes and their volatile compounds were also evaluated on the mycelial growth of the pathogen. To evaluate the effect of HP in the suppressiveness to Cylindrocladium spathiphylli, HP was added in potting mix artificially infested, at concentrations of 0, 10, 20, 30, 40 and 50% of the volume of water required to reach the water retention capacity of the potting mix. The mixtures were incubated for 10 days and transferred to pots containing one plug of Spathiphyllum Opal per pot. In the experiments in vitro, aqueous extracts and mixtures containing HP showed the highest suppressiveness against the pathogen. In the Spathiphyllum growing, the suppressiveness occurred at concentrations higher than 20% of fish hidrolyzed.

Histologia e histoquímica do intestino anterior de tilápia do Nilo (Oreochromis niloticus) alimentadas com dietas contendo silagem de peixe

This work was carried out to evaluate the action of the fermented fish silage and fish meal in the histochemical of the middle intestine of O. niloticus. A great epithelium intestinal was present in fishes fed with fish silage, independent of the animal origin protein proportions in diets. It was observed that the variation of the goblet cells secretion glicoproteic intensity is linked directly to the diet supplied to animals. The middle intestine of Oreochromis niloticus showed differences in the neutral mucus glicoproteic, acid glicoproteic and glicoconjugated secretion pattern, depending on the protein origin and the percentage used in the diet, demonstrating that this species can adapt its secretion system for the protection of the digesting apparatus during the absorption of different protein sources.