Adubação fosfatada, produção e desempenho em campo de sementes de feijoeiro cv. Carioca Precoce e IAC Carioca Tybatã

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Uma experiência de plantão psicológico no CTI: semera e acolher

Este estudo avalia a implantação e desenvolvimento do Serviço de Plantão Psicológico em um Centro de Terapia Intensiva - CTI de um hospital universitário vinculado a rede pública de saúde, na capital paraense. O serviço foi disponibilizado aos familiares de pacientes internados e demais membros da equipe de saúde intensivista, funcionando na antessala do referido setor, duas vezes por semana, durante quatro meses. Alicerçado sob os pilares da Abordagem Centrada na Pessoa (ACP) buscou-se compreender os pressupostos teórico-metodológicos que fundamentam essa modalidade de cuidado, as especificidades do setting no que se referem aos objetivos, ações e funções do plantonista, assim como, as urgências reveladas neste contexto. Para tanto, elegeu-se como método do estudo a pesquisa qualitativa de base fenomenológica, sendo avaliadas as trajetórias do semear e germinar do Plantão Psicológico. São analisados seis casos clínicos, os quais lançam luz sobre essa modalidade de atenção psicológica no CTI. Quanto ao perfil da clientela atendida se observou que essa foi composta predominantemente por familiares, mulheres entre 20 a 75 anos, em média com o Ensino Fundamental e renda de um salário mínimo mensal. Os resultados indicam a necessidade e viabilidade da oferta do Plantão Psicológico no CTI, as demandas urgentes por auxílio psicológico, desveladas nos sentidos que os clientes atribuíram as suas experiências, tais como, medo de que o familiar faleça, sensação de abandono do familiar, culpa por não poder permanecer ao seu lado, tristeza intensa em razão do estado de saúde ou quando do óbito, entre outros. Dois tipos de atendimentos foram, naturalmente, criados: o individual e o grupal, sendo consideradas as especificidades das demandas. Ressalta-se também quanto a esta modalidade a disponibilização do pronto atendimento as urgências, acolhimento e estímulo a comunicação. Portanto, considera-se que a oferta do Plantão Psicológico no CTI revelou-se necessária como um espaço de cuidado psíquico aceito, utilizado e legitimado pelos clientes, além de se configurar em dois momentos distintos, antes e após ás visitas, sendo que no primeiro destes, destacam-se as intervenções voltadas ao acolhimento e fortalecimento da organização do self, enquanto no segundo, aquelas voltadas a ajudar os clientes na ressignificação de suas experiências ameaçadoras e a reorganização do self.

Instrumento cortador e removedor de material edodôntico: desenvolvimento e aplicabilidade

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Políticas públicas municipais de saúde: fortalecendo a democracia em pequenos municípios brasileiros

This article presents the endeavors of health municipal teams in building services that meet citizens’ demands, in a small municipality from a less developed area of the state of São Paulo. Beyond health services practice characterization and organization, our study assesses the challenges and advances in the implementation of public, health policies in Brazil. In this way it is possible to perceive how the family health strategy needs to overcome impasses in the service work processes, as well as more dynamic local and regional relations to provide comprehensive care to citizens. Health care exemplifies how, in democracy building, municipal action is indispensable to strengthen the effectiveness of public actions.

Talent Management in Higher Education: Developing Emerging Leaders Within the Administration at Private Colleges and Universities

This research focused on identifying a series of successful practices relating to administrative talent management within the higher education setting. The field study included a thorough examination of seven small to mid-size private colleges and universities that have incorporated employee development strategies. These strategies were aimed at growing future leaders from within the organization in order to achieve continuity and support institutional priorities. Specifically, several focus areas were investigated including presidential vision, leadership commitment, talent management’s place among institutional priorities, program characteristics, and program evaluation. Among the commonalities that were gathered included support at the senior officer level who serve as advocates, mentors, and program facilitators, a strong connection between talent management and the institutions’ strategic plans, and a holistic approach to developing talent at all levels of the organizations. In addition, both coaching and opportunities for growth in the work environment were evident within several of the institutions. Also, academic leadership development was considered to be a part of the talent management strategy within three of the colleges and universities. The key differentiators included the incorporation of organizational and leadership competencies to provide focus toward the performance development process at two institutions, the implementation of a succession planning model at another institution, and the location of human resource generalists in departments across two of the institutions to identify learning opportunities for both individuals and work teams. Based on both the findings from the field study and the literature review, a comprehensive procedural model is introduced that serves to support human resource departments and higher education professionals, in general, who are looking to either begin or broaden their own talent management approach. However, despite the progress that has been made across several institutions noted throughout the research study, much more must be learned in terms of how the time and resources invested in talent management translates to institutional success. Advisor: James O‘Hanlon

Antinuclear antibodies testing as a routine screening for systemic lupus erythematosus in patients presenting first-episode psychosis

Aims: This report discusses the use of antinuclear antibody (ANA) detection as a screening test for neuropsychiatry systemic lupus erythematosus (NPSLE) in patients presenting a first-episode psychosis. Methods: We reviewed the medical records of 85 patients admitted to an emergency service due to first-episode psychosis, during a 1-year period, for whom ANA detection was performed through an IFI HEp2 cell assay. ANA-positive patients were subsequently evaluated for autoantibodies and neuroimaging exams. Results: Three patients presented as ANA positive in the initial screening and further investigation confirmed NPSLE in two patients. The patients were treated with antipsychotics and cyclophosphamide pulses with satisfactory outcomes. Conclusion: Even though ANA detection is not specific, it is a low-cost procedure and could be an important screening test for NPSLE in the early-onset psychosis.

O processo comunicativo no serviço de atendimento móvel de urgência (SAMU-192)

Este trabalho objetiva caracterizar o processo comunicativo entre os auxiliares de enfermagem das viaturas de Suporte Básico de Vida do Serviço de Atendimento Móvel de Urgência, a coordenação deste serviço e a Central Única de Regulação Médica de um município do Estado de São Paulo. Trata-se de uma pesquisa descritivo-qualitativa que utilizou a análise temática de conteúdo para a análise dos dados. Usou-se entrevista semi-estruturada para a coleta de dados que foi realizada em janeiro de 2010. Os resultados obtidos mostram dificuldades de comunicação tanto com a Central de Regulação Médica quanto com a coordenação. Os aspectos que mais se destacaram foram as falhas durante a transmissão via rádio, falta de capacitação dos operadores de rádio, acesso à coordenação dificultado e ausência da supervisão dos enfermeiros. No entanto, foi possível detectar soluções que visam à melhoria da comunicação e, consequentemente, do atendimento ofertado pelo Serviço de Atendimento Móvel de Urgência.

Aerobic biodegradation of chlorinated solvents and plastics in batch and continuous-flow bioreactors: process development, and identification of suitable chemical pre-Treatments

The purpose of the first part of the research activity was to develop an aerobic cometabolic process in packed bed reactors (PBR) to treat real groundwater contaminated by trichloroethylene (TCE) and 1,1,2,2-tetrachloroethane (TeCA). In an initial screening conducted in batch bioreactors, different groundwater samples from 5 wells of the contaminated site were fed with 5 growth substrates. The work led to the selection of butane as the best growth substrate, and to the development and characterization from the site’s indigenous biomass of a suspended-cell consortium capable to degrade TCE with a 90 % mineralization of the organic chlorine. A kinetic study conducted in batch and continuous flow PBRs and led to the identification of the best carrier. A kinetic study of butane and TCE biodegradation indicated that the attached-cell consortium is characterized by a lower TCE specific degredation rates and by a lower level of mutual butane-TCE inhibition. A 31 L bioreactor was designed and set up for upscaling the experiment. The second part of the research focused on the biodegradation of 4 polymers, with and with-out chemical pre-treatments: linear low density polyethylene (LLDPE), polyethylene (PP), polystyrene (PS) and polyvinyl chloride (PVC). Initially, the 4 polymers were subjected to different chemical pre-treatments: ozonation and UV/ozonation, in gaseous and aqueous phase. It was found that, for LLDPE and PP, the coupling UV and ozone in gas phase is the most effective way to oxidize the polymers and to generate carbonyl groups on the polymer surface. In further tests, the effect of chemical pretreatment on polyner biodegrability was studied. Gas-phase ozonated and virgin polymers were incubated aerobically with: (a) a pure strain, (b) a mixed culture of bacteria; and (c) a fungal culture, together with saccharose as a co-substrate.

Evaluation of respiratory parameters at presentation as clinical indicators of the respiratory localization in dogs and cats with respiratory distress

OBJECTIVE: To describe clinical respiratory parameters in cats and dogs with respiratory distress and identify associations between respiratory signs at presentation and localization of the disease with particular evaluation between the synchrony of abdominal and chest wall movements as a clinical indicators for pleural space disease. Design - Prospective observational clinical study. SETTING: Emergency service in a university veterinary teaching hospital. ANIMALS: Cats and dogs with respiratory distress presented to the emergency service between April 2008 and July 2009. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: The following parameters were systematically determined at time of admission: respiratory rate, heart rate, temperature, type of breathing, movement of the thoracic and abdominal wall during inspiration, presence of stridor, presence and type of dyspnea, and results of thoracic auscultation. Abdominal and chest wall movement was categorized as synchronous, asynchronous, or inverse. Diagnostic test results, diagnosis, and outcome were subsequently recorded. Based on the final diagnoses, animals were assigned to 1 or more of the following groups regarding the anatomical localization of the respiratory distress: upper airways, lower airways, lung parenchyma, pleural space, thoracic wall, nonrespiratory causes, and normal animals. One hundred and seventy-six animals (103 cats and 73 dogs) were evaluated. Inspiratory dyspnea was associated with upper airway disease in dogs and expiratory dyspnea with lower airway disease in cats. Respiratory noises were significantly associated and highly sensitive and specific for upper airway disease. An asynchronous or inverse breathing pattern and decreased lung auscultation results were significantly associated with pleural space disease in both dogs and cats (P<0.001). The combination is highly sensitive (99%) but not very specific (45%). Fast and shallow breathing was not associated with pleural space disease. Increased or moist pulmonary auscultation findings were associated with parenchymal lung disease. CONCLUSIONS: Cats and dogs with pleural space disease can be identified by an asynchronous or inverse breathing pattern in combination with decreased lung sounds on auscultation.

Isolated vertebral fractures give elevated serum protein S-100B levels

ABSTRACT: BACKGROUND: Serum protein S-100B determinations have been widely proposed in the past as markers of traumatic brain injury and used as a predictor of injury severity and outcome. The purpose of this prospective observational case series was therefore to determine S-100B serum levels in patients with isolated injuries to the back. METHODS: Between 1 February and 1 May 2008, serum samples for S-100B analysis were obtained within 1 hour of injury from 285 trauma patients. All patients with a head injury, polytrauma, and intoxicated patients were excluded to select isolated injuries to the spine. 19 patients with isolated injury of the back were included. Serum samples for S-100B analysis and CT spine were obtained within 1 hours of injury. RESULTS: CT scans showed vertebral fractures in 12 of the 19 patients (63%). All patients with fractures had elevated S-100B levels. Amongst the remaining 7 patients without a fracture, only one patient with a severe spinal contusion had an S-100B concentration above the reference limit. The mean S-100B value of the group with fractures was more than 4 times higher than in the group without fractures (0.385 vs 0.087 mug/L, p = 0.0097). CONCLUSION: Our data, although limited due to a very small sample size, suggest that S-100B serum levels might be useful for the diagnosis of acute vertebral body and spinal cord injury with a high negative predictive power. According to the literature, the highest levels of serum S-100B are found when large bones are fractured. If a large prospective study confirms our findings, determining the S-100B level may contribute to more selective use of CT and MRI in spinal trauma.

Clinical presentation of a traumatic cervical spine disc rupture in alpine sports: a case report

ABSTRACT: Isolated non-skeletal injuries of the cervical spine are rare and frequently missed. Different evaluation algorithms for C-spine injuries, such as the Canadian C-spine Rule have been proposed, however with strong emphasis on excluding osseous lesions. Discoligamentary injuries may be masked by unique clinical situations presenting to the emergency physician. We report on the case of a 28-year-old patient being admitted to our emergency department after a snowboarding accident, with an assumed hyperflexion injury of the cervical spine. During the initial clinical encounter the only clinical finding the patient demonstrated, was a burning sensation in the palms bilaterally. No neck pain could be elicited and the patient was not intoxicated and did not have distracting injuries. Since the patient described a fall prevention attempt with both arms, a peripheral nerve contusion was considered as a differential diagnosis. However, a high level of suspicion and the use of sophisticated imaging (MRI and CT) of the cervical spine, ultimately led to the diagnosis of a traumatic disc rupture at the C5/6 level. The patient was subsequently treated with a ventral microdiscectomy with cage interposition and ventral plate stabilization at the C5/C6 level and could be discharged home with clearly improving symptoms and without further complications.This case underlines how clinical presentation and extent of injury can differ and it furthermore points out, that injuries contracted during alpine snow sports need to be considered high velocity injuries, thus putting the patient at risk for cervical spine trauma. In these patients, especially when presenting with an unclear neurologic pattern, the emergency doctor needs to be alert and may have to interpret rigid guidelines according to the situation. The importance of correctly using CT and MRI according to both - standardized protocols and the patient's clinical presentation - is crucial for exclusion of C-spine trauma.

Volume 11, Number 3: September/October 2005

Page 2 – The University and its Libraries celebrate 125 years of growth and service to the citizens of Connecticut. • UConn joins the Center for Research Libraries, the nation’s oldest and most significant library consortium. Page 3 - A profile of Stanley Israelite recounts his civic contributions and his role as a creator of the Dodd Research Center. Page 4 - The Thomas J. Dodd Research Center celebrates its 10th anniversary with a series of special events. • The Pharmacy Library occupies new quarters in UConn’s new Pharmacy/Biology Building. Page 5 - The 2005 Connecticut Children’s Book Fair features a great lineup of authors and illustrators. Page 6 - Staff News • The Wharton Data Research Service is now available for financial research. Page 7 - Donors to the University Libraries, January 1 – June 30, 2005

The regulation of mTORC2 kinase activity and complex integrity

Growth factor signaling promotes anabolic processes via activation of the PI3K-Akt kinase cascade. Deregulation of the growth factor-dependent PI3K-Akt pathway was implicated in tumorigenesis. Akt is an essential serine/threonine protein kinase that controls multiple physiological functions such as cell growth, proliferation, and survival to maintain cellular homeostasis. Recently, the mammalian Target of Rapamycin Complex 2 (mTORC2) was identified as the main Akt Ser-473 kinase, and Ser-473 phosphorylation is required for Akt hyperactivation. However, the detailed mechanism of mTORC2 regulation in response to growth factor stimulation or cellular stresses is not well understood. In the first project, we studied the regulation of the mTORC2-Akt signaling under ER stress. We identified the inactivation of mTORC2 by glycogen synthase kinase-3β (GSK-3β). Under ER stress, the essential mTORC2 component, rictor, is phosphorylated by GSK-3β at Ser-1235. This phosphorylation event results in the inhibition of mTORC2 kinase activity by interrupting Akt binding to mTORC2. Blocking rictor Ser-1235 phosphorylation can attenuate the negative impacts of GSK-3β on mTORC2/Akt signaling and tumor growth. Thus, our work demonstrated that GSK-3β-mediated rictor Ser-1235 phosphorylation in response to ER stress interferes with Akt signaling by inhibiting mTORC2 kinase activity. In the second project, I investigated the regulation of the mTORC2 integrity. We found that basal mTOR kinase activity depends on ATP level, which is tightly regulated by cell metabolism. The ATP-sensitive mTOR kinase is required for SIN1 protein phosphorylation and stabilization. SIN1 is an indispensable subunit of mTORC2 and is required for the complex assembly and mTORC2 kinase activity. Our findings reveal that mTOR-mediated phosphorylation of SIN1 is critical for maintaining complex integrity by preventing SIN1 from lysosomal degradation. In sum, our findings verify two distinct mTORC2 regulatory mechanisms via its components rictor and SIN1. First, GSK-3β-mediated rictor Ser-1235 phosphorylation results in mTORC2 inactivation by interfering its substrate binding ability. Second, mTOR-mediated Ser-260 phosphorylation of SIN1 preserves its complex integrity. Thus, these two projects provide novel insights into the regulation of mTORC2.

Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the alpha subunit of the receptor.

The experimental manipulation of peptide growth hormones and their cellular receptors is central to understanding the pathways governing cellular signaling and growth control. Previous work has shown that intracellular antibodies targeted to the endoplasmic reticulum (ER) can be used to capture specific proteins as they enter the ER, preventing their transport to the cell surface. Here we have used this technology to inhibit the cell surface expression of the alpha subunit of the high-affinity interleukin 2 receptor (IL-2R alpha). A single-chain variable-region fragment of the anti-Tac monoclonal antibody was constructed with a signal peptide and a C-terminal ER retention signal. Intracellular expression of the single-chain antibody was found to completely abrogate cell surface expression of IL-2R alpha in stimulated Jurkat T cells. IL-2R alpha was detectable within the Jurkat cells as an immature 40-kDa form that was sensitive to endoglycosidase H, consistent with its retention in a pre- or early Golgi compartment. A single-chain antibody lacking the ER retention signal was also able to inhibit cell surface expression of IL-2R alpha although the mechanism appeared to involve rapid degradation of the receptor chain within the ER. These intracellular antibodies will provide a valuable tool for examining the role of IL-2R alpha in T-cell activation, IL-2 signal transduction, and the deregulated growth of leukemic cells which overexpress IL-2R alpha.

Avaliação da contaminação conjunta de Dekkera bruxellensis e Lactobacillus fermentum sobre a fermentação alcoólica: efeito do substrato e formas de controle

As contaminações por leveduras selvagens e por bactérias no processo de produção de etanol combustível no Brasil causam prejuízos ao rendimento fermentativo e aumento de custos pelo uso de biocidas. No entanto, poucos estudos tem focado no efeito das contaminações conjuntas de leveduras selvagens e bactérias e as possíveis interações entre os micro-organismos, especialmente em função dos diferentes substratos de fermentação e das formas de controle. Este trabalho teve por objetivos verificar o efeito do substrato (caldo de cana e melaço) sobre o desenvolvimento das contaminações pela levedura da espécie Dekkera bruxellensis e pela bactéria Lactobacillus fermentum, em co-culturas com Saccharomyces cerevisiae (linhagem industrial PE-2) e possíveis formas de controle do crescimento dos contaminantes (pelo uso de metabissulfito de potássio e adição de etanol ao tratamento ácido) sem afetar a levedura do processo. Os testes foram realizados em condições de crescimento (substrato com 4 °Brix, culturas agitadas) e fermentação com reciclo celular (substrato com 16 °Brix, culturas estáticas). Houve interação entre as leveduras e a bactéria quando crescidas em caldo de cana 4 °Brix. A levedura industrial não foi afetada pela presença dos micro-organismos contaminantes, no entanto, para D. bruxellensis a presença de L. fermentum interferiu positivamente no crescimento, com aumento no número de UFC, e consequentemente inibição do crescimento da bactéria. Em melaço, houve um estímulo ao crescimento de L. fermentum quando em co-cultura com S. cerevisiae. Houve influência das contaminações sobre os parâmetros avaliados no experimento (pH, açúcar redutor total, etanol, glicerol e crescimento das células) e a contaminação conjunta de L. fermentum e D. bruxellensis potencializou o efeito das contaminações pelos micro-organismos isoladamente, tanto em caldo quanto em melaço. A adição de 13% de etanol à solução de ácido sulfúrico pH 2,0 no tratamento celular resultou em uma diminuição significativa no número de UFC de D. bruxellensis (entre 90-99%). A levedura PE-2 foi pouco afetada pelo tratamento proposto. A bactéria L. fermentum teve seu crescimento afetado em todas as combinações testadas. Como os experimentos foram feitos em co-culturas, verificouse que pode haver influência de um micro-organismo sobre a viabilidade do outro, dependendo da reação ao tratamento ácido-etanol. O metabissulfito de potássio (MBP), no intervalo entre 200-400 mg/L, foi eficaz para controlar o crescimento de D. bruxellensis dependendo do meio de cultura e linhagem. Quando adicionado (250 mg/L) à solução ácida (pH 2,0) no tratamento celular, um efeito significativo foi observado nas culturas mistas, pois ocorreu a inativação do SO2 pela S. cerevisiae e uma provável proteção das células de D. bruxellensis, não sendo essa levedura prejudicada pelo MBP. A resposta fisiológica de S. cerevisiae na presença de MBP pode explicar a diminuição significativa na produção de etanol. Quando o MBP foi adicionado ao meio de fermentação, resultou no controle da D. bruxellensis mas não em sua morte, com efeito menos intensivo sobre a eficiência fermentativa. Em cocultura com a adição de MBP, a eficiência fermentativa foi significativamente menor do que na ausência de MBP.