Systematic Reverse Genetics of Transfer-DNA-Tagged Lines of Arabidopsis1 : Isolation of Mutations in the Cytochrome P450 Gene Superfamily

We have developed an efficient reverse-genetics protocol that uses expedient pooling and hybridization strategies to identify individual transfer-DNA insertion lines from a collection of 6000 independently transformed lines in as few as 36 polymerase chain reactions. We have used this protocol to systematically isolate Arabidopsis lines containing insertional mutations in individual cytochrome P450 genes. In higher plants P450 genes encode enzymes that perform an exceptionally wide range of functions, including the biosynthesis of primary metabolites necessary for normal growth and development, the biosynthesis of secondary products, and the catabolism of xenobiotics. Despite their importance, progress in assigning enzymatic function to individual P450 gene products has been slow. Here we report the isolation of the first 12 such lines, including one (CYP83B1-1) that displays a runt phenotype (small plants with hooked leaves), and three insertions in abundantly expressed genes. The DNAs used in this study are publicly available and can be used to systematically isolate mutants in Arabidopsis.

Gibbs Energy of Mixing Function: Topological Analysis in Azeotropic Systems

ESAT 2014. 27th European Symposium on Applied Thermodynamics, Eindhoven University of Technology, July 6-9, 2014.

Standard hand-book of household economy for the people : comprising plain directions for the management of a family, servants, lying-in room, nursery, sick-room, flower-garden, and household pets, and also, for the preparation and administration of remedies for disease /

Includes index.

An analysis of transfer and development lengths for pretensioned concrete structures /

"December 1994."

United States of America, petitioner, v. Standard Oil Company of New Jersey et al., defendants.

At head of title: In the Circuit Court of the United States for the Eastern Division of the Eastern Judicial District of Missouri.

State architect's standard construction specifications of November 1, 1955.

Mode of access: Internet.

United States of America, petitioner, v. Standard oil company of New Jersey et al., defendants ...

Vol. 22 and vol. A [v. 23] contain the "Transcript of record" in this case on appeal to the Supreme court of the United States, October term, 1909, no. 725, and are printed by Judd & Detweiler (inc.), printers, Washington, D.C., 1910.

The Standard library atlas of classical geography, completed to the present state of knowledge. With a general index giving the latitude & longitude of every place named in the maps.

WHS shelf-list has note: Inv. 29.

England's Aegis, or, The military energies of the constitution.

Mode of access: Internet.

State Architect's standard mechanical specifications of November 1, 1955.

"Contained herein are general conditions, forms of proposal, agreements and bonds, State Architect's standard specifications for mechanical, electric and elevator works."

Wages (standard time rates). Board of trade (Labour department). Report on standard time rates of wages in the United Kingdom in 1900; with comparative tables.

Continued by another publication, with same title, October, 1906 (Cd. 3245).

The Napoleon anecdotes : illustrating the mental energies of the late Emperor of France, and the character and actions of his contemporary statesmen and warriors /

Mode of access: Internet.

The total and free energies of formation of the oxides of thirty-two metals,

Bibliographical foot-notes.

The Napoleon anecdotes : illustrating the mental energies of the late emperor of France; and the characters and actions of his contemporary statesmen and warriors /

Mode of access: Internet.