Hierarchical self-assembly of the DNA-grafted supramolecular polymers

Conjugation of functional entities with a specific set of optical, mechanical or biological properties to DNA strands allows engineering of sophisticated DNA-containing architectures. Among various hybrid systems, DNA-grafted polymers occupy an important place in modern materials science. In this contribution we present the non-covalent synthesis and properties of DNA-grafted linear supramolecular polymers (SPs), which are assembled in a controllable manner from short chimeric DNA-pyrene oligomers. The synthetic oligomers consist of two parts: a 10 nucleotides long DNA chain and a covalently attached segment of variable number of phosphodiester-linked pyrenes. The temperature-dependent formation of DNA-grafted SPs is described by a nucleation-elongation mechanism. The high tendency of pyrenes to aggregate in water, leads to the rapid formation of SPs. The core of the assemblies consists of stacked pyrenes. They form a 1D platform, to which the DNA chains are attached. Combined spectroscopic and microscopic studies reveal that the major driving forces of the polymerization are π-stacking of pyrenes and hydrophobic interactions, and DNA pairing contributes to a lesser extent. AFM and TEM experiments demonstrate that the 1D SPs appear as elongated ribbons with a length of several hundred nanometers. They exhibit an apparent helical structure with a pitch-to-pitch distance of 50±15 nm. Since DNA pairing is a highly selective process, the ongoing studies are aimed to utilize DNA-grafted SPs for the programmable arrangement of functional entities. For example, the addition of non-modified complementary DNA strands to the DNA-grafted SPs leads to the cooperative formation of higher-order assemblies. Also, our experiments suggest that the fluorescent pyrene core of 1D ribbons serves as an efficient donor platform for energy transfer applications.

Hierarchical self-assembly of the DNA-grafted supramolecular polymers

Conjugation of functional entities with a specific set of optical, mechanical or biological properties to DNA strands allows engineering of sophisticated DNA-containing architectures. Among various hybrid systems, DNA-grafted polymers occupy an important place in modern materials science. In this contribution we present the non-covalent synthesis and properties of DNA-grafted linear supramolecular polymers (SPs), which are assembled in a controllable manner from short chimeric DNA-pyrene oligomers. The synthetic oligomers consist of two parts: a 10 nucleotides long DNA chain and a covalently attached segment of variable number of phosphodiester-linked pyrenes. The temperature-dependent formation of DNA-grafted SPs is described by a nucleation-elongation mechanism. The high tendency of pyrenes to aggregate in water, leads to the rapid formation of SPs. The core of the assemblies consists of stacked pyrenes. They form a 1D platform, to which the DNA chains are attached. Combined spectroscopic and microscopic studies reveal that the major driving forces of the polymerization are π-stacking of pyrenes and hydrophobic interactions, and DNA pairing contributes to a lesser extent. AFM and TEM experiments demonstrate that the 1D SPs appear as elongated ribbons with a length of several hundred nanometers. They exhibit an apparent helical structure with a pitch-to-pitch distance of 50±15 nm. Since DNA pairing is a highly selective process, the ongoing studies are aimed to utilize DNA-grafted SPs for the programmable arrangement of functional entities. For example, the addition of non-modified complementary DNA strands to the DNA-grafted SPs leads to the cooperative formation of higher-order assemblies. Also, our experiments suggest that the fluorescent pyrene core of 1D ribbons serves as an efficient donor platform for energy transfer applications.

Of missing fathers and Big Fish: reconstructive tendencies after postmodernism

Many things have been said about literature after postmodernism, but one point there seems to be some agreement on is that it does not turn its back radically on its postmodernist forerunner, but rather generally continues to heed and value their insights. There seems to be something strikingly non-oedipal about the recent aesthetic shift. It is a project of reconstruction that remains deeply rooted in postmodernist tenets. Such an essentially non-oedipal attitude, I would argue, is central to the nature of the reconstructive shift. This, however, also raises questions about the wider cultural context from which such an aesthetic stance arises. If postmodernism was nurtured by the revolutionary spirits of the late 1960s, reconstruction faces a different world with different strategies. Instead of the postmodernist urge to subvert, expose and undermine, reconstruction yearns towards tentative and fragile intersubjective understanding, towards responsibility and community. Instead of revolt and rebellion it explores reconciliation and compromise. One instance in which this becomes visible in reconstructive narratives is the recurring figure of the lost father. Missing father figures abound in recent novels by authors like Mark Z. Danielewski, Dave Eggers, Yann Mantel, David Mitchell etc. It almost seems like a younger generation is yearning for the fathers which postmodernism has struggled hard to do away with. My paper will focus on one particularly striking example to explore the implications of this development: Daniel Wallace’s novel Big Fish and Tim Burton’s well-known film adaptation of the same. In their negotiation of fact and fiction, of doubt and belief, of freedom and responsibility, all of which converge in a father-son relationship, they serve well to illustrate central characteristics and concerns of recent attempts to leave postmodernism behind.

Denying the foreseeability of an event as a means of self-protection. The Impact of Self-Threatening Outcome Information on the Strength of the Hindsight Bias

The hindsight bias represents the tendency of people to falsely believe that they would have predicted the outcome of an event, once the outcome is known. Two experiments will be presented that show a reduction or even reversal of the hindsight bias when the outcome information is self-threatening for the participants. Participants read a report of an interaction between a man and a woman that ended with different outcomes: The woman was raped vs. the woman was not raped vs. no outcome information was given. Results of the first experiment indicated that especially female participants, who did not accept rape myths, showed a reversed hindsight bias, when they received the rape outcome information. The more threatening the rape outcome had been, the lower was their estimated likelihood of rape. Results of the second experiment confirmed those of the first. Female participants, who did not accept rape myths and perceived themselves highly similar to the victim, showed a strong reversed hindsight bias, when threatened by the rape outcome, whereas female participants, who did believe in rape myth and were not similar to the victim, showed a classical hindsight bias. These effects were interpreted in terms of self-serving or in-group serving functions of the hindsight bias: Participants deny the foreseeability of a self-threatening outcome as a means of self-protection even if they are not personally affected by the negative information, but a member of their group.

Exculpating victims and the reversal of hindsight bias

This experiment examines the role of the hindsight bias and of motivational forces such as the motive to believe in a just world as possible causes of the derogation of victims effect in the context of rape. The hindsight bias is the tendency of people to falsely believe that they would have predicted the outcome of an event once the outcome is known. Participants read descriptions of an interaction between a man and a woman that ended with one of four possible outcomes: The woman was raped with very severe consequences for her future life vs. rape with only minor consequences for her future life vs. no rape (assailant was forced to retreat by the strong defense of the victim) vs. no outcome information. To test motivational predictions the hindsight bias and the derogation effect were analyzed as a consequence of the sex of participants, the seriousness of the consequences of the rape, the belief in a just world and the acceptance of rape myths. Results supported the assumption that derogation effects are at least partly driven by hindsight bias and that motivational processes work via the hindsight bias. However, in this study we did not find a classical hindsight bias but a reversed hindsight bias: Especially female participants in the severe consequences of rape condition and those participants who did not accept rape myths rated the likelihood of rape in the rape outcome condition as smaller than participants in the no outcome information control group. They also derogated the victim less than participants in the no information control group. These effects were interpreted in terms of self-serving or in-group serving functions of the hindsight bias. Finally no support was found for the assumption that derogation effects are driven by the motive to believe in a just world.

Overcoming Liability of Newness through Socialization : How Legitimation Strategies of a New Venture evolve

Liability of newness, the tendency of new ventures to die early after market entry, results from lacking legitimacy in their new cultural context and according failure to acquire resources. Based on a longitudinal case study on repeated resource acquisition attempts of a new venture, we found that overcoming liability of newness depended on the socialization of the new venture to the normative environment on which it depended on for resources. Over time and across repeated resource acquisition attempts, socialization - the process of learning the use of legitimate symbols and their culturally contingent meanings - enabled the new venture to become the skillful cultural operator on which legitimation and resource acquisition was contingent. From our data, 'Accumulating a repertoire of legitimate symbols' and 'Assimilating the evaluations of resource-holders' emerged as the two primary mechanisms for new venture socialization. The study's contributions to related literature and its broader theoretical implications are discussed

Social views of distant university

"La sociedad tiende a fundarse cada vez más en el conocimiento", expresaba la Conferencia Mundial sobre Educación Superior (París, 1998). "Por consiguiente -concluía- la educación superior ha de emprender la renovación más radical que jamás haya tenido por delante...". Hoy sólo el 1% de la humanidad accede a la Universidad, durante un período de su vida. El desafío que enfrentamos es que pueda acceder el 99% restante, durante toda su vida. Sostenemos que la plataforma de comunicación y las tecnologías para el perfil que asumirá la universidad en el nuevo siglo ya existen y se desenvolverán aceleradamente en la próxima década...

Tab. 1: Number and percentage of glacier movements in the Austrian Alps between 1960 and 1975

The statistical record of the length of Austrian glaciers is continued with an improved classification scheme. The tendency of increasing glacier advance is maintained since 1965. 54 glaciers, or 58 % of the 93 observed, were advancing in 1975. A relation that is noticed between the behavior of the terminus and mean air temperature of the ablation period is discussed in qualitative terms.

Las Fuerzas Armadas Revolucionarias : Orígenes y desarrollo de una particular conjunción entre marxismo, peronismo y lucha armada [1960-1973]

Desde mediados de la década del cincuenta hasta la última dictadura militar, la Argentina vivió un período de gran conflictividad social y política. Particularmente desde el Cordobazo de 1969, amplios sectores de la clase trabajadora, el campo cultural, la iglesia y el movimiento estudiantil protagonizaron un intenso proceso de politización, dando lugar a un conjunto de movimientos de oposición de diverso orden. Las organizaciones armadas, al desafiar el monopolio estatal de la violencia legítima y establecer diversos lazos con el movimiento de protesta social más amplio, fueron uno de los actores destacados de ese proceso. Entre ellas, las 'Fuerzas Armadas Revolucionarias' [FAR] condensan varias problemáticas de relevancia en el período: el proceso de identificación con el peronismo de numerosos sectores de izquierda, la reivindicación de la violencia como forma de intervención política y la opción por la lucha armada como modalidad específica de ponerla en práctica. Pese a su importancia, hasta el momento no se había realizado ningún trabajo académico específico sobre esta organización. La presente tesis analiza los orígenes y el desarrollo de las FAR considerando el período que va desde los primeros sesenta, en que comenzaron a perfilarse sus grupos fundadores, hasta las elecciones presidenciales del 11 de marzo de 1973. Desde entonces no sólo cambia notablemente la dinámica política nacional, sino que la realidad de la organización ya está signada por el proceso de fusión con Montoneros, que fue anunciada de modo público en octubre de ese año. El problema de investigación articula dos ejes analíticos. Por un lado, el proceso de identificación de las FAR con el peronismo, cuyos antecedentes se remontan a las sucesivas reinterpretaciones realizadas por sus grupos fundadores sobre el fenómeno. El segundo, con su dinámica de funcionamiento como organización político-militar de actuación nacional y urbana, gestada al calor de los cambios de estrategias que se plantearon aquellos grupos fundadores para lograr el proceso de liberación nacional y social que impulsaban. Ambas líneas de análisis implican, además, adentrarse en los modos en que la organización concibió sus vínculos con sectores más amplios de la sociedad, particularmente con aquellos que buscaba movilizar. Para realizar la tesis se apeló a una estrategia metodológica cualitativa. Se relevaron fuentes escritas [diarios y revistas de alcance nacional, documentos de las FAR y de otras organizaciones con que se vincularon] y se realizaron entrevistas semiestructuradas a ex-miembros de la organización

Las Fuerzas Armadas Revolucionarias : Orígenes y desarrollo de una particular conjunción entre marxismo, peronismo y lucha armada [1960-1973]

Desde mediados de la década del cincuenta hasta la última dictadura militar, la Argentina vivió un período de gran conflictividad social y política. Particularmente desde el Cordobazo de 1969, amplios sectores de la clase trabajadora, el campo cultural, la iglesia y el movimiento estudiantil protagonizaron un intenso proceso de politización, dando lugar a un conjunto de movimientos de oposición de diverso orden. Las organizaciones armadas, al desafiar el monopolio estatal de la violencia legítima y establecer diversos lazos con el movimiento de protesta social más amplio, fueron uno de los actores destacados de ese proceso. Entre ellas, las 'Fuerzas Armadas Revolucionarias' [FAR] condensan varias problemáticas de relevancia en el período: el proceso de identificación con el peronismo de numerosos sectores de izquierda, la reivindicación de la violencia como forma de intervención política y la opción por la lucha armada como modalidad específica de ponerla en práctica. Pese a su importancia, hasta el momento no se había realizado ningún trabajo académico específico sobre esta organización. La presente tesis analiza los orígenes y el desarrollo de las FAR considerando el período que va desde los primeros sesenta, en que comenzaron a perfilarse sus grupos fundadores, hasta las elecciones presidenciales del 11 de marzo de 1973. Desde entonces no sólo cambia notablemente la dinámica política nacional, sino que la realidad de la organización ya está signada por el proceso de fusión con Montoneros, que fue anunciada de modo público en octubre de ese año. El problema de investigación articula dos ejes analíticos. Por un lado, el proceso de identificación de las FAR con el peronismo, cuyos antecedentes se remontan a las sucesivas reinterpretaciones realizadas por sus grupos fundadores sobre el fenómeno. El segundo, con su dinámica de funcionamiento como organización político-militar de actuación nacional y urbana, gestada al calor de los cambios de estrategias que se plantearon aquellos grupos fundadores para lograr el proceso de liberación nacional y social que impulsaban. Ambas líneas de análisis implican, además, adentrarse en los modos en que la organización concibió sus vínculos con sectores más amplios de la sociedad, particularmente con aquellos que buscaba movilizar. Para realizar la tesis se apeló a una estrategia metodológica cualitativa. Se relevaron fuentes escritas [diarios y revistas de alcance nacional, documentos de las FAR y de otras organizaciones con que se vincularon] y se realizaron entrevistas semiestructuradas a ex-miembros de la organización

Las Fuerzas Armadas Revolucionarias : Orígenes y desarrollo de una particular conjunción entre marxismo, peronismo y lucha armada [1960-1973]

Desde mediados de la década del cincuenta hasta la última dictadura militar, la Argentina vivió un período de gran conflictividad social y política. Particularmente desde el Cordobazo de 1969, amplios sectores de la clase trabajadora, el campo cultural, la iglesia y el movimiento estudiantil protagonizaron un intenso proceso de politización, dando lugar a un conjunto de movimientos de oposición de diverso orden. Las organizaciones armadas, al desafiar el monopolio estatal de la violencia legítima y establecer diversos lazos con el movimiento de protesta social más amplio, fueron uno de los actores destacados de ese proceso. Entre ellas, las 'Fuerzas Armadas Revolucionarias' [FAR] condensan varias problemáticas de relevancia en el período: el proceso de identificación con el peronismo de numerosos sectores de izquierda, la reivindicación de la violencia como forma de intervención política y la opción por la lucha armada como modalidad específica de ponerla en práctica. Pese a su importancia, hasta el momento no se había realizado ningún trabajo académico específico sobre esta organización. La presente tesis analiza los orígenes y el desarrollo de las FAR considerando el período que va desde los primeros sesenta, en que comenzaron a perfilarse sus grupos fundadores, hasta las elecciones presidenciales del 11 de marzo de 1973. Desde entonces no sólo cambia notablemente la dinámica política nacional, sino que la realidad de la organización ya está signada por el proceso de fusión con Montoneros, que fue anunciada de modo público en octubre de ese año. El problema de investigación articula dos ejes analíticos. Por un lado, el proceso de identificación de las FAR con el peronismo, cuyos antecedentes se remontan a las sucesivas reinterpretaciones realizadas por sus grupos fundadores sobre el fenómeno. El segundo, con su dinámica de funcionamiento como organización político-militar de actuación nacional y urbana, gestada al calor de los cambios de estrategias que se plantearon aquellos grupos fundadores para lograr el proceso de liberación nacional y social que impulsaban. Ambas líneas de análisis implican, además, adentrarse en los modos en que la organización concibió sus vínculos con sectores más amplios de la sociedad, particularmente con aquellos que buscaba movilizar. Para realizar la tesis se apeló a una estrategia metodológica cualitativa. Se relevaron fuentes escritas [diarios y revistas de alcance nacional, documentos de las FAR y de otras organizaciones con que se vincularon] y se realizaron entrevistas semiestructuradas a ex-miembros de la organización

Geochemistry and mineralogy of ODP Leg 107 basalts

During ODP Leg 107, the basement of the Tyrrhenian Sea was drilled at Site 650, located in the Marsili basin, and at Sites 651 and 655, both located in the Vavilov basin. In addition, a lava flow was drilled at Site 654 on the Sardinia rifted margin. Mineral and whole rock major and trace element chemistry, including rare earth element (REE) and Sr and Nd isotopic ratios, were determined in samples of these rocks. Site 654 lava was sampled within uppermost Pliocene postrift sediments. This lava is a basaltic andesite of intraplate affinity, and is analogous to some Plio-Pleistocene tholeiitic lavas from Sardinia. Site 650 basalts, drilled beneath 1.7-1.9-Ma-old basal sediment, are strongly altered and vesicular suggesting a rapid subsidence of the Marsili basin. Based on incompatible trace elements, these basalts show calc-alkaline affinity like some products of the Marsili Seamount and the Eolian arc. The basement of the two sites drilled within Vavilov basin shows contrasting petrologies. Site 655, located along the Gortani ridge in the western part of the basin, drilled a 116-m-thick sequence of basalt flows beneath 3.4-3.6-Ma-old basal sediments. These basalts are chemically relatively homogeneous and show affinity to transitional MORB. Four units consisting of slightly differentiated basaltic lavas, have been identified. Site 655 basalts are geochemically similar to the high Ti lavas from DSDP Leg 42, Site 373 (Vavilov Basin). The basement at Site 651, overlain by 40 m of metalliferous dolostone covered by fossiliferous sediments with an age of 2 Ma, consists of two basalt units separated by a dolerite-albitite intrusive body; serpentinized harzburgites were drilled for 30 m at the base of the hole. The two basalt units of Site 651 are distinct petrochemically, though both show incompatible elements affinity with high-K calc-alkaline/calc-alkaline magmas from Eolian arc. The cpx chemistry and high K/Na ratio of the lower unit lavas suggest a weak alkaline tendency of potassic lineage. Leg 107 basement rock data, together with data from DSDP Site 373 and from dredged samples, indicate that the deepest basins of the central Tyrrhenian Sea are underlain by a complex back-arc basin crust produced by magmas with incompatible element affinities to transitional MORB (Site 655 and DSDP Site 373), and to calc-alkaline and high-K calc-alkaline converging plate margin basalts (Sites 650 and 651). This petrogenetic complexity is in accordance with the back-arc setting of the Vavilov and Marsili basins. Other back-arc basin basalts, particularly those from ensialic basins such as the Bransfield Strait (Antarctica), show a comparable petrogenetic complexity (cf., Sounders and Tarney, 1984).

(Table 1) Lake elevation change derived from 4 or more years of measurements

In this study, ICESat altimetry data are used to provide precise lake elevations of the Tibetan Plateau (TP) during the period of 2003-2009. Among the 261 lakes examined ICESat data are available on 111 lakes: 74 lakes with ICESat footprints for 4-7 years and 37 lakes with footprints for 1 -3 years. This is the first time that precise lake elevation data are provided for the 111 lakes. Those ICESat elevation data can be used as baselines for future changes in lake levels as well as for changes during the 2003-2009 period. It is found that in the 74 lakes (56 salt lakes) examined, 62 (i.e. 84%) of all lakes and 50 (i.e. 89%) of the salt lakes show tendency of lake level increase. The mean lake water level increase rate is 0.23 m/year for the 56 salt lakes and 0.27 m/year for the 50 salt lakes of water level increase. The largest lake level increase rate (0.80 m/year) found in this study is the lake Cedo Caka. The 74 lakes are grouped into four subareas based on geographical locations and change tendencies in lake levels. Three of the four subareas show increased lake levels. The mean lake level change rates for subareas I, II, III, IV, and the entire TP are 0.12, 0.26, 0.19, -0.11, and 0.2 m/year, respectively. These recent increases in lake level, particularly for a high percentage of salt lakes, supports accelerated glacier melting due to global warming as the most likely cause.

Communication peculiarities of early-aged children from speech risk group with close grownups

More children with different versions of speech disorders appear in Russia last decades. This situation reflects general tendency of national health deterioration. Our practical experience shows that close grownups can?t communicate to children with limited health capacity. As a result there arise social disabilities in child development. Speech communication is one of the forms of global communicative interaction process between close grownups and young child in the course of which there is a redistribution of knowledge and ignorance (Nikas Luman,2005). Within a framework of sociocultiral theory of mental growth we consider the appearance of speech communication under any cases of physical illness is possible only under conditions of correctly- organized communication between grownups and young children. (L.S. Vigotski ,2000). The special value in this aspect acquires the study of communication between grownups and young children. For five years we have been conducting the surveys on the problem of communicative contacts between parents and non-verbal children. Analysis of received data gave us the opportunity to systematize peculiar communicative interaction of adults and children who have some lapses in acquiring speech form communication. We have revealed four versions of situational- business communication between close grownups and young children with disabilities in acquiring speech. We have assumed that four versions of situational- business communication negatively affect speech form communication formation.

Development of an Elevated Temperature Loop Heat Pipe for Space Applications and Investigation of Non Condensable Gas Impact on its Performance

Un caloducto en bucle cerrado o Loop Heat Pipe (LHP) es un dispositivo de transferencia de calor cuyo principio de operación se basa en la evaporación/condensación de un fluido de trabajo, que es bombeado a través de un circuito cerrado gracias a fuerzas de capilaridad. Gracias a su flexibilidad, su baja masa y su mínimo (incluso nulo) consumo de potencia, su principal aplicación ha sido identificada como parte del subsistema de control térmico de vehículos espaciales. En el presente trabajo se ha desarrollado un LHP capaz de funcionar eficientemente a temperaturas de hasta 125 oC, siguiendo la actual tendencia de los equipos a bordo de satélites de incrementar su temperatura de operación. En la selección del diseño optimo para dicho LHP, la compatibilidad entre materiales y fluido de trabajo se identificó como uno de los puntos clave. Para seleccionar la mejor combinación, se llevó a cabo una exhaustiva revisión del estado del arte, además de un estudio especifico que incluía el desarrollo de un banco de ensayos de compatibilidad. Como conclusión, la combinación seleccionada como la candidata idónea para ser integrada en el LHP capaz de operar hasta 125 oC fue un evaporador de acero inoxidable, líneas de titanio y amoniaco como fluido de trabajo. En esa línea se diseñó y fabricó un prototipo para ensayos y se desarrolló un modelo de simulación con EcosimPro para evaluar sus prestaciones. Se concluyó que el diseño era adecuado para el rango de operación definido. La incompatibilidad entre el fluido de trabajo y los materiales del LHP está ligada a la generación de gases no condensables. Para un estudio más detallado de los efectos de dichos gases en el funcionamiento del LHP se analizó su comportamiento con diferentes cantidades de nitrógeno inyectadas en su cámara de compensación, simulando un gas no condensable formado en el interior del dispositivo. El estudio se basó en el análisis de las temperaturas medidas experimentalmente a distintos niveles de potencia y temperatura de sumidero o fuente fría. Adicionalmente, dichos resultados se compararon con las predicciones obtenidas por medio del modelo en EcosimPro. Las principales conclusiones obtenidas fueron dos. La primera indica que una cantidad de gas no condensable más de dos veces mayor que la cantidad generada al final de la vida de un satélite típico de telecomunicaciones (15 años) tiene efectos casi despreciables en el funcionamiento del LHP. La segunda es que el principal efecto del gas no condensable es una disminución de la conductancia térmica, especialmente a bajas potencias y temperaturas de sumidero. El efecto es más significativo cuanto mayor es la cantidad de gas añadida. Asimismo, durante la campaña de ensayos se observó un fenómeno no esperado para grandes cantidades de gas no condensable. Dicho fenómeno consiste en un comportamiento oscilatorio, detectado tanto en los ensayos como en la simulación. Este efecto es susceptible de una investigación más profunda y los resultados obtenidos pueden constituir la base para dicha tarea. ABSTRACT Loop Heat Pipes (LHPs) are heat transfer devices whose operating principle is based on the evaporation/condensation of a working fluid, and which use capillary pumping forces to ensure the fluid circulation. Thanks to their flexibility, low mass and minimum (even null) power consumption, their main application has been identified as part of the thermal control subsystem in spacecraft. In the present work, an LHP able to operate efficiently up to 125 oC has been developed, which is in line with the current tendency of satellite on-board equipment to increase their operating temperatures. In selecting the optimal LHP design for the elevated temperature application, the compatibility between the materials and working fluid has been identified as one of the main drivers. An extensive literature review and a dedicated trade-off were performed, in order to select the optimal combination of fluids and materials for the LHP. The trade-off included the development of a dedicated compatibility test stand. In conclusion, the combination of stainless steel evaporator, titanium piping and ammonia as working fluid was selected as the best candidate to operate up to 125 oC. An LHP prototype was designed and manufactured and a simulation model in EcosimPro was developed to evaluate its performance. The first conclusion was that the defined LHP was suitable for the defined operational range. Incompatibility between the working fluid and LHP materials is linked to Non Condensable Gas (NCG) generation. Therefore, the behaviour of the LHP developed with different amounts of nitrogen injected in its compensation chamber to simulate NCG generation, was analyzed. The LHP performance was studied by analysis of the test results at different temperatures and power levels. The test results were also compared to simulations in EcosimPro. Two additional conclusions can be drawn: (i) the effects of an amount of more than two times the expected NCG at the end of life of a typical telecommunications satellite (15 years) is almost negligible on the LHP operation, and (ii) the main effect of the NCG is a decrease in the LHP thermal conductance, especially at low temperatures and low power levels. This decrease is more significant with the progressive addition of NCG. An unexpected phenomenon was observed in the LHP operation with large NCG amounts. Namely, an oscillatory behaviour, which was observed both in the tests and the simulation. This effect provides the basis for further studies concerning oscillations in LHPs.