The isolation, characterization, and application of WRKY genes as useful molecular markers in tropical trees

The improvement of tropical tree crops using conventional breeding methods faces challenges due to the length of time involved. Thus, like most crops, there is an effort to utilize molecular genetic markers in breeding programs to select for desirable agronomic traits. Known as marker assisted breeding or marker assisted selection, genetic markers associated with a phenotype of interest are used to screen and select material reducing the time necessary to evaluate candidates. As the focus of this research was improving disease resistance in tropical trees, the usefulness of the WRKY gene superfamily was investigated as candidates for generating useful molecular genetic markers. WRKY genes encode plant-specific transcriptional factors associated with regulating plants' responses to both biotic and abiotic stress. ^ One pair of degenerate primers amplified 48 WRKY gene fragments from three taxonomically distinct, economically important, tropical tree crop species: 18 from Theobroma cacao L., 21 from Cocos nucifera L. and 9 from Persea americana Mill. Several loci from each species were polymorphic because of single nucleotide substitutions present within a putative non-coding region of the loci. Capillary array electrophoresis-single strand conformational polymorphism (CAE-SSCP) mapped four WRKY loci onto a genetic linkage map of a T. cacao F2 population segregating for resistance to witches' broom disease. Additionally, PCR primers specific for four T. cacao loci successfully amplified WRKY loci from 15 members of the Byttneriae tribe. A method was devised to allow the reliable discrimination of alleles by CAE-SSCP using only the mobility assigned to the sample peaks. Once this method was validated, the diversity of three WRKY loci was evaluated in a germplasm collection of T. cacao . One locus displayed high diversity in the collection, with at least 18 alleles detected from mobility differences of the product peaks. The number of WRKY loci available within the genome, ease of isolation by degenerate PCR, codominant segregation demonstrated in the F2 population, and usefulness for screening germplasm collections and closely related wild species demonstrates that the WRKY superfamily of genes are excellent candidates for developing a number of genetic molecular markers for breeding purposes in tropical trees. ^

Water temperature reconstructions based on paired Uk'37 and Tex86 records, and paired surface and subsurface Mg/Ca records

This dataset contains the collection of available published paired Uk'37 and Tex86 records spanning multi-millennial to multi-million year time scales, as well as a collection of Mg/Ca-derived temperatures measured in parallel on surface and subsurface dwelling foraminifera, both used in the analyses of Ho and Laepple, Nature Geoscience 2016. As the signal-to-noise ratios of proxy-derived Holocene temperatures are relatively low, we selected records that contain at least the last deglaciation (oldest sample >18kyr BP).

The Proceedings of the South Carolina Historical Association

Annually, the association publishes a journal, The Proceedings, which consists of papers presented at the annual meeting. Rev. Philip Mulkey, Pioneer Baptist Preacher in Upper South Carolina by Floyd Mulkey –Consolidated Book Publishers, Chicago, Ill. The South Carolina Ordnance Board, 1860-1861 by Frank E. Vandiver Some Events of the American Revolution in South Carolina as Recorded by the Rev. James Jenkins by George F. Scheer – The University of North Carolina Press

Letter from James Winthrop to William Bentley, 1788 February 27

Two leaves containing a one-page handwritten letter from Winthrop to Bentley that mentions Rev. John Prince (1751-1836) and briefly discussing the state of Winthrop's microscope and five volumes presented to Harvard that were printed in Calcutta, India.

The autobiography of the Rev. William Jay : with reminiscences of some distinguished contemporaries, selections from his correspondence, etc. /

Includes index.

William James, Sciences of Mind, and Anti-imperial Discourse

In the past few decades, the humanities and social sciences have developed new methods of reorienting their conceptual frameworks in a “world without frontiers.” In this book, Bernadette M. Baker offers an innovative approach to rethinking sciences of mind as they formed at the turn of the twentieth century, via the concerns that have emerged at the turn of the twenty-first. The less-visited texts of Harvard philosopher and psychologist William James provide a window into contemporary debates over principles of toleration, anti-imperial discourse, and the nature of ethics. Baker revisits Jamesian approaches to the formation of scientific objects including the child mind, exceptional mental states, and the ghost to explore the possibilities and limits of social scientific thought dedicated to mind development and discipline formation around the construct of the West.

Early promoted : a memoir of the Rev. William Spiller Cox by Edward W Cox

http://www.archive.org/details/earlypromotedame00coxwuoft

An American missionary : a record of the work of Rev. William H. Judge, S.J. / by Charles J. Judge ; introduction by His Eminence, Cardinal Gibbons.

http://www.archive.org/details/anamericanmissio00judguoft

Letter - William Young to James Woodruff

Letter to James D. Woodruff at the Welland Canal, St. Catharines. The letter is postmarked Philadelphia [date illegible] and Queenston, Feb. 7, 1847. In this letter William Young of Philadelphia describes some of the prices and features of his wares including compasses and levels, Feb. 3, 1847.