Colonization pattern of Cecropia by Azteca ants: Influence of plant ontogeny, environment and host plant choice by queens

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Nicotiana tabacum as an experimental host for the study of plant-Xylella fastidiosa interactions

Xylella fastidiosa causes citrus variegated chlorosis (CVC). Information generated from the X. fastidiosa genome project is being used to study the underlying mechanisms responsible for pathogenicity. However, the lack of an experimental host other than citrus to study plant-X. fastidiosa interaction has been an obstacle to accelerated progress in this area. We present here results of three experiments that demonstrated that tobacco could be an important experimental host for X. fastidiosa. All tobacco plants inoculated with a citrus strain of X. fastidiosa expressed unequivocal symptoms, consisting of orange leaf lesions, approximately 2 months after injection of the pathogen. CVC symptoms were observed in citrus 3 to 6 months after inoculation. The pathogen was readily detected in symptomatic tobacco plants by polymerase chain reaction (PCR) and phase contrast microscopy. In addition, X. fastidiosa was reisolated on agar plates in 4 of 10 plants. Scanning electron microscopy analysis of cross sections of stems and petioles revealed the presence of rod shaped bacteria restricted to the xylem of inoculated plants. The cell size was within the limit typical of X. fastidiosa.

Chromosome analysis of two species of sugar cane pests of the genus Mahanarva (Homoptera, Cercopidae)

Studies on the cytogenetics of Homoptera are scarce. Some references in the literature have reported a chromosome number for the genus ranging from n=5 to 19 and 2n=10 to 2n=39 chromosomes. The genus Mahanarva includes two species of agricultural importance as pests of sugar cane culture in Brazil. We report here the first data concerning the chromosome number and morphology of the species Mahanarva fimbriolata and M. posticata. The chromosome number observed for the two species was 2n=19 for males and 2n=20 for females. The sex determining mechanism of these species was of the XX/X0 type (for males and females, respectively), with chromosome X being the smallest in the complement.

The effect of plant diversity on fungus garden development and foraging behavior of leaf-cutting ants (Hymenoptera: Formicidae)

The leaf-cutting ants forage a wide variety of plant species, used for symbiotic fungus cultivation. To better understand this tripartite complex interaction, 24 colonies of Acromyrmex subterraneus brunneus were conditioned for 4 months to 6 different plants (Citrus spp., Ligustrum spp., Acalypha spp., Eucalyptus spp., Alchornea triplinervia, Melia spp.), to verify the influence of conditioning on foraging behavior of workers. The effect of plants on symbiotic fungus development was studied separately, through macerated plants in Agar and culture medium A as the control. During foraging, workers presented polyphagic foraging behavior, refusing the plants to which they were conditioned. The selection of plants is not correlated with the plant substrate that promotes good development of symbiotic fungus. Such results demonstrate the importance of plant diversity for fungus garden maintenance.

Occurrence of plant-parasitic nematodes in ornamental and flowering plants at UNESP/FCAV, campus of jaboticabal, são paulo state, brazil

The plant-parasitic nematodes are responsible for serious injuries in roots and shoots of ornamental plants, reducing its beauty and consequently its economic value. This study aimed to ascertain the occurrence and distribution of plantparasitic nematodes through the analysis of the roots of ornamental and flowering plants at UNESP FCAV's landscape. The roots were collected from fifteen different species as follows: Anthurium andreannum, Rhododendron simsii, Impatiens walleriana, Calathea stromata, Cordyline terminalis, Dieffenbachia picta, Dracaena marginata, Ficus benjamina, Spathiphyllum ortgiesii 'Sensation', Spathiphyllum wallisi 'American Beauty' and 'Mini', Odontonema strictum, Portulaca grandiflora, Strelitzia reginae, Tradescantia zebrina and Tradescantia pallida. Samples of roots were processed. The plant-parasitic nematodes identified in the samples were: Meloidogyne sp. (Anthurium andreannum, Calathea stromata, Dieffenbachia picta, Ficus benjamina, Impatiens walleriana, Odontonema strictum, Portulaca grandiflora, Spathiphyllum ortgiesii 'Sensation'), Helicotylenchus dihystera (Calathea stromata, Dracaena marginata, Portulaca grandiflora, Spathiphyllum ortgiessi 'Sensation', Tradescantia pallida, Tradescantia zebrina), Tylenchus sp. (Anthurium andreannum, Calathea stromata, Cordyline terminalis, Dieffenbachia picta, Ficus benjamina, Rhododendron simsii), Aphelenchoides sp. (Dieffenbachia picta, Spathiphyllum ortgiesii 'Sensation', S. wallisi 'American Beauty'), Rotylenchulus reniformis (Cordyline terminalis, Dracaena marginata, Odontonema strictum), Pratylenchus sp. (Spathiphyllum ortgiesii 'Sensation', Spathiphyllum wallisi 'Mini'), Ditylenchus sp. (Spathiphyllum wallisi 'Mini'), Pratylenchus brachyurus (Tradescantia zebrina). The plant-parasitic nematodes weren't found in the roots of Strelitzia reginae.

Effect of plant density on Stevia rebaudiana Bertoni water consumption

Stevia rebaudiana is a traditional crop of Latin America, now rising to international interest thanks to its specific glycosides that are very sweet and still poor of calories making its potential interesting for dietary recommendations. Its short growing cycle makes this species possible as an annual crop also in non tropical areas, including the Mediterranean Basin and parts of Italy, where rainfall or irrigation is available during late spring and summer. One of the main concerns is the quantity of water necessary for the crop and the ideal number of plants per hectare in order to maximize water use efficiency. The present research was carried out in central Italy to compare yields and water consumption in plots with three plant densities (50, 25, 16 plants per square metre). The results show that the most dense plots can give the best results in the area of the trial.

Desiccation tolerance in seeds of Annona emarginata (Schldtl.) H. Rainer and action of plant growth regulators on germination

Annona emarginata (Schldtl.) H. Rainer (araticum-de-terra-fria) is used as a rootstock for several species of Annonaceae. It is suggested that these seeds should be sown immediately after extraction and, therefore, they could be intolerant to desiccation. There are several mechanisms involved with desiccation tolerance. Soluble sugars, for example, can accumulate and act as osmoprotectants for the membrane system during desiccation. The aim of this study is to assess desiccation tolerance in A. emarginata seeds. In addition, we examined the soluble sugars involved in desiccation tolerance. Finally, we determined the effect of gibberellic acid (GA4+7) and N-(phenylmethyl)-aminopurine in promoting the germination of seeds with different water contents. The experiment consisted of a randomized 4×5 factorial design (desiccation levels × concentration of growth regulators). After drying, seeds containing 31 (control), 19, 12 and 5% water were incubated in different concentrations of GA4+7 N-(phenylmethyl)-aminopurine (0, 250, 500, 750 and 1000 mg L-1) for 60 hours. The experiment was conducted in a germination chamber with alternating temperature and photoperiod of 20oC for 18 hours of darkness and 30oC for 6 hours of light. We analyzed electrical conductivity, germination rate, mean germination time, germination speed, frequency and uniformity of germination, percentage of dormant seeds and soluble sugar profile in intact seeds through high-performance liquid chromatography (HPLC). The data were subjected to analysis of variance, and the means were compared using Tukey's test at a threshold of p<0.05. The results showed that seeds of A. emarginata appears to be desiccation tolerant and, also, that sucrose increases when seed water content is reduced to values as low as 12%, exogenous GA4+7+N-(phenylmethyl)-aminopurine improves its germinability.

Anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity

Although tannin-rich forages are known to increase protein uptake and to reduce gastrointestinal nematode infections in grazing ruminants, most published research involves forages with condensed tannins (CT), while published literature lacks information on the anthelmintic capacity, nutritional benefits, and antioxidant capacity of alternative forages containing hydrolyzable tannins (HT). We evaluated the anthelmintic activity and the antioxidant capacity of plant extracts containing either mostly CT, mostly HT, or both CT and HT. Extracts were prepared with 70% acetone, lyophilized, redissolved to doses ranging from 1.0mg/mL to 25mg/mL, and tested against adult Caenorhabditis elegans as a test model. The extract concentrations that killed 50% (LC50) or 90% (LC90) of the nematodes in 24h were determined and compared to the veterinary anthelmintic levamisole (8mg/mL). Extracts were quantified for CT by the acid butanol assay, for HT (based on gallic acid and ellagic acid) by high-performance liquid chromatography (HPLC) and total phenolics, and for their antioxidant activity by the oxygen radical absorbance capacity (ORAC) assay. Extracts with mostly CT were Lespedeza cuneata, Salix X sepulcralis, and Robinia pseudoacacia. Extracts rich in HT were Acer rubrum, Rosa multiflora, and Quercus alba, while Rhus typhina had both HT and CT. The extracts with the lowest LC50 and LC90 concentrations, respectively, in the C. elegans assay were Q. alba (0.75 and 1.06mg/mL), R. typhina collected in 2007 (0.65 and 2.74mg/mL), A. rubrum (1.03 and 5.54mg/mL), and R. multiflora (2.14 and 8.70mg/mL). At the doses of 20 and 25mg/mL, HT-rich, or both CT- and HT-rich, extracts were significantly more lethal to adult C. elegans than extracts containing only CT. All extracts were high in antioxidant capacity, with ORAC values ranging from 1800μmoles to 4651μmoles of trolox equivalents/g, but ORAC did not correlate with anthelmintic activity. The total phenolics test had a positive and highly significant (r=0.826, p≤0.01) correlation with total hydrolyzable tannins. Plants used in this research are naturalized to the Appalachian edaphoclimatic conditions, but occur in temperate climate areas worldwide. They represent a rich, renewable, and unexplored source of tannins and antioxidants for grazing ruminants, whereas conventional CT-rich forages, such as L. cuneata, may be hard to establish and adapt to areas with temperate climate. Due to their high in vitro anthelmintic activity, antioxidant capacity, and their adaptability to non-arable lands, Q. alba, R. typhina, A. rubrum, and R. multiflora have a high potential to improve the health of grazing animals and must have their anthelmintic effects confirmed in vivo in both sheep and goats. © 2012.

Effects of plant cover on the macrofauna of Spartina marshes in northern Brazil

Dados sobre a densidade e diversidade da macrofauna em relação à altura e densidade de Spartina brasiliensis foram obtidos em bancos de marismas em um estuário tropical no norte do Brasil. A amostragem foi realizada quatro vezes durante um ano, nas estações chuvosa, seca e nos períodos de transição entre estas. A amostragem foi realizada em marismas de três classes de tamanho: pequeno, médio e grande. As variáveis foram analisadas em relação às estações do ano e das classes de tamanho das marismas. Um total de 46 táxons foram encontrados, com os poliquetos, isopodos e o gastropódo Neritina virginea dominando a fauna, resultados similares a estudos realizados em marismas no sul do Brasil. A densidade e a diversidade da macrofauna foram correlacionadas positivamente com a densidade de colmos da vegetação, indicando um possível papel da vegetação em proteção contra predação. Todas as três variáveis foram maiores durante os períodos transicionais entre as estações chuvosa e seca e mudanças sazonais em precipitação, salinidade e disponibilidade de luz possam influenciar mortalidade, disponibilidade de alimento e assentamento da macrofauna. Não houve um efeito de tamanho da marisma sobre a macrofauna ou a vegetação. O efeito beneficial da vegetação sobre a macrofauna é apoiado por outros estudos de marismas brasileiras.

Intraspecific variability of Holostylis reniformis: concentration of lignans, as determined by maceration and supercritical fluid extraction (SFE-CO2), as a function of plant provenance and plant parts

Maceration and supercritical fluid extraction were used to prepare extracts from parts of plants (Holostylis reniformis) collected in two different regions of Brazil. ¹H NMR, HPLC-DAD-ESI/MS, HPLC-DAD, GC-MS, and chemometric techniques were used to analyse lignans in the extracts and showed that yields of SFE-CO2 were less than or equal to those of hexane maceration extracts. These analyses, in conjunction with the concentrations of aliphatic hydrocarbons, fatty acids and their methyl and ethyl derivatives in the extracts, also allowed the chemical composition of parts and provenance of the plant to be differentiated.

Effect of plant regulators on growth and flowering of 'Meyer' zoysiagrass

This trial aimed to evaluate the effect of sequential applications of different plant regulators over growth and flower rachis emission of 'Meyer' zoysiagrass (Zoysia japonica). The study was conducted on 15-month old green turfgrass under a randomized complete block design with four replications. The following plant regulator and doses were tested: trinexapac-ethyl (113+113, 226+113, 226+226, 452+113, 452+226, 452+452, 678+339 e 904+452 g a.i./ha-1), prohexadione-calcium (100+100 e 200+200 g a.i. ha-1) and bispyribac-sodium (40+40 e 60+60 g a.i. ha-1), as well as an untreated control. The turfgrass was mowed again at 3.0 cm aboveground and the second plant regulator was applied when 'Meyer' zoysiagrass was between 5.0 and 6.0 cm high. The effect of the treatments was visually rated for visual injury, plant height, height and number of flower rachis, and total dry mass production of clippings. Only bispyribac-sodium had visual symptoms of injury on 'Meyer' zoysiagrass, and no intoxication was observed at 28 days after the second application (DAAB). The sequential applications of trinexapac-ethyl, prohexadione-calcium and bispyribac-sodium reduced by more than 80% the total clipping dry mass produced by 'Meyer' zoysiagrass. All the plant regulators tested also showed promising results in reducing the height and emission of rachis, especially when trinexapac-ethyl was applied at the doses 452+452, 678+339 and 904+452 g a.i. ha-1. 'Meyer' zoysiagrass turfgrass can be handled with the sequential application of a plant regulator, which reduces the need for mowing over a period up to 110 days after the application of the second plant regulator, and it also avoids deleterious visual effects over turfgrass.

Antioxidant and intestinal anti-inflammatory effects of plant-derived coumarin derivatives

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Systematic Screening of Plant Extracts from the Brazilian Pantanal with Antimicrobial Activity against Bacteria with Cariogenic Relevance

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Beta Diversity of Plant-Pollinator Networks and the Spatial Turnover of Pairwise Interactions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)