899 resultados para Image recognition and processing
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Mode of access: Internet.
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Includes bibliographical references (p. 658-666).
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Description based on: Crop of 1959 (Aug. 3, 1959).
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February 1994.
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Mode of access: Internet.
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Microfiche sheets have title: Clinical diagnosis of selected diseases exotic to most of the Americas.
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Includes bibliographical references.
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Mode of access: Internet.
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Includes bibliographical references.
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Mode of access: Internet.
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"Application deadline: December 19, 2007"
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Includes bibliographical references.
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Cover title.
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Modifications at the N-terminus of the rabbit CYP4B1 gene resulted in expression levels in Escherichia coli of up to 660 nmol/L. Solubilization of the enzyme from bacterial membranes led to substantial conversion to cytochrome P420 unless alpha-naphthoflavone was added as a stabilizing ligand. Mass spectrometry analysis and Edman sequencing of purified enzyme preparations revealed differential N-terminal post-translational processing of the various constructs expressed. Notably, bacterial expression of CYP4B1 produced a holoenzyme with >98.5% of its heme prosthetic group covalently linked to the protein backbone. The near fully covalently linked hernoproteins exhibited similar rates and regioselectivities of lauric acid hydroxylation to that observed previously for the partially heme processed enzyme expressed in insect cells. These studies shed new light on the consequences of covalent heme processing in CYP4B1 and provide a facile system for future mechanistic and structural studies with the enzyme. (C) 2003 Elsevier Science (USA). All rights reserved.