Fibroma uterino en una hembra canina cruzada de 12 años en Veterinaria Vida, Noviembre Managua, Nicaragua 2013

Se analizó un estudio de caso atendido en la Clínica “VIDA ” el 21 de noviembre del 2013. Inicialmente se procedió a la valoración de su estado físico y de salud considerando la edad de la paciente. A partir de la inspección clínica y anamnesis se procedió a brindar un diagnóstico de la patología que presentaba, para fundamentar las principales causas y consecuencia que gen eran las patologías reproductivas en la especie canina. El estudio de caso es una investigación cualitativa de tipo participativa, cuya base es la descripción de sucesos ocurridos en un momento determinado el cual consistía en el análisis de un estudio de caso que no pueden duplicarse por las condiciones individuales del sujeto y de su entorno en particular . El paciente era una perra de 12 años , llamada princesa que t enía completo su programa de vacunación y desparasitación. Después del último celo tuvo descargas vaginal es hemorrágicas , durante 6 días el cual alarmó a la dueña, ya que no mostró síntomas característicos de postraciones . Era alimentada con concentrado, su estro era normal . Se le brindó atención ambulatoria y s e le realizaron exámenes clínicos , realizando la anamnesis , sin mostrar dolor a la palpación, no se detectaron masas abdominales, no se realizó exploración vagina l debido a que la paciente no habí a sido incorporada para reproducción. Se determinó como piómetra en diagnostico presu ntivo, aplicándole vit. K p or tres días y al cuarto día una dosis de medroxiprogesterona para detener el sangrado. A l no haber efecto en el sangrado, se optó por la extirpación quirúrgica del útero el cual se real izó sin problemas, encontrándose: cuernos uterinos y útero hiperplásicos , endometrio con fibromas y pequeñas neoplasias. Se identificó neoplasia uterina causada por quistes ováricos, estrés, trastorno psicológico de adaptabilidad a la ausencia de la dueña, creando un desorde n en las estructuras de cascada del sistema endocrino, viéndose afectado por la relació n de la edad de la paciente. Terminada la cirugía se le entregó la mascota a su dueña junto con las indicaciones postoperatoria pero ya en su casa la paciente murió por complicaciones post quirúrgica no identificadas .

Estudio epidemiológico retrospectivo de neoplasias diagnosticadas por histopatología en caninos en una clínica veterinaria de Bogotá D. C.

Tesis (Médico Veterinario). -- Universidad de La Salle. Facultad de Ciencias Agropecuarias. Programa de Medicina Veterinaria, 2014

Estudio retrospectivo de los hallazgos clínicos diagnosticados con ayuda de radiología digital según las historias clínicas de los años 2010 a 2012 en la clínica veterinaria Dover de Bogotá

Tesis (Médico Veterinario). -- Universidad de La Salle. Facultad de Ciencias Agropecuarias. Programa de Medicina Veterinaria, 2015

Determinación de la prevalencia serológica de brucella abortus, toxoplasma gondii, sarcocystis spp y los factores de riesgo asociados en estudiantes de medicina veterinaria de la Universidad de la Salle

Tesis (Médico Veterinario). -- Universidad de La Salle. Facultad de Ciencias Agropecuarias. Programa de Medicina Veterinaria, 2013

Hallazgos radiográficos torácicos, en caninos de la clínica veterinaria ULS desde 2002 al 2011

Tesis (Médico Veterinario). -- Universidad de La Salle. Facultad de Ciencias Agropecuarias. Programa de Medicina Veterinaria, 2014

Estudio retrospectivo de caballos con síndrome de abdomen agudo diagnosticados con desplazamiento de colon dorsal derecho en el centro de veterinaria y zootecnia de La Universidad Ces durante el período 2005-2013

Tesis (Médico Veterinario). -- Universidad de La Salle. Facultad de Ciencias Agropecuarias. Programa de Medicina Veterinaria, 2014

Characterising the spatial dynamics of sympatric Aedes aegypti and Aedes albopictus populations in the Philippines

Entomological surveillance and control are essential to the management of dengue fever (DF). Hence, understanding the spatial and temporal patterns of DF vectors, Aedes (Stegomyia) aegypti (L.) and Ae. (Stegomyia) albopictus (Skuse), is paramount. In the Philippines, resources are limited and entomological surveillance and control are generally commenced during epidemics, when transmission is difficult to control. Recent improvements in spatial epidemiological tools and methods offer opportunities to explore more efficient DF surveillance and control solutions: however, there are few examples in the literature from resource-poor settings. The objectives of this study were to: (i) explore spatial patterns of Aedes populations and (ii) predict areas of high and low vector density to inform DF control in San Jose village, Muntinlupa city, Philippines. Fortnightly, adult female Aedes mosquitoes were collected from 50 double-sticky ovitraps (SOs) located in San Jose village for the period June-November 2011. Spatial clustering analysis was performed to identify high and low density clusters of Ae. aegypti and Ae. albopictus mosquitoes. Spatial autocorrelation was assessed by examination of semivariograms, and ordinary kriging was undertaken to create a smoothed surface of predicted vector density in the study area. Our results show that both Ae. aegypti and Ae. albopictus were present in San Jose village during the study period. However, one Aedes species was dominant in a given geographic area at a time, suggesting differing habitat preferences and interspecies competition between vectors. Density maps provide information to direct entomological control activities and advocate the development of geographically enhanced surveillance and control systems to improve DF management in the Philippines.

Construction of microsatellite linkage maps for Corymbia

The genus Corymbia is closely related to the genus Eucalyptus, and like Eucalyptus contains tree species that are important for sub-tropical forestry. Corymbia's close relationship with Eucalyptus suggests genetic studies in Corymbia should benefit from transfer of genetic information from its more intensively studied relatives. Here we report a genetic map for Corymbia spp. based on microsatellite markers identified de novo in Corymbia sp or transferred from Eucalyptus. A framework consensus map was generated from an outbred F 2 population (n = 90) created by crossing two unrelated Corymbia torelliana x C. citriodora subsp. variegata F1 trees. The map had a total length of 367 cM (Kosambi) and was composed of 46 microsatellite markers distributed across 13 linkage groups (LOD 3). A high proportion of Eucalyptus microsatellites (90%) transferred to Corymbia. Comparative analysis between the Corymbia map and a published Eucalyptus map identified eight homeologous linkage groups in Corymbia with 13 markers mapping on one or both maps. Further comparative analysis was limited by low power to detect linkage due to low genome coverage in Corymbia, however, there was no convincing evidence for chromosomal structural differences because instances of non-synteny were associated with large distances on the Eucalyptus map. Segregation distortion was primarily restricted to a single linkage group and due to a deficit of hybrid genotypes, suggesting that hybrid inviability was one factor shaping the genetic composition of the F2 population in this inter-subgeneric hybrid. The conservation of microsatellite loci and synteny between Corymbia and Eucalyptus suggests there will be substantial value in exchanging information between the two groups.

Genetic variation within two sympatric spotted gum eucalypts exceeds between taxa variation

Population substructure and hybridization, among other factors, have the potential to cause erroneous associations in linkage disequilibrium (LD) mapping. Two closely related spotted gum eucalypts, Corymbia variegata and C. henryi (Myrtaceae) occur in sympatry in the east coast of Australia and potentially interbreed. They are morphologically similar but are distinguished as separate species based on capsule and foliage size. To determine whether they hybridize in nature and its implications for LD mapping, we investigated the level of molecular divergence between the two species at two sympatric locations separated by 300 kilometres. Very few individuals of intermediate morphology were identified, despite the two species occurring only metres apart. Analysis of genetic structure using 12 microsatellite loci showed that genetic differentiation between populations of the same species at different locations (FST = 0.07 for both species; p = 0.0001) was significantly higher than that observed between species at each location (mean FST = 0.02 and 0.04 for Cherry tree and Bunyaville respectively; p = 0.0001; all Mann-Whitney U-test p ≤ 0.01). No species-specific alleles or significant allele frequency differences were detected within a site, suggesting recurrent local gene flow between the two species. The lack of significant allele frequency differences implies no population stratification along taxonomic lines. This suggested that there is little concern for cryptic hybridization when sampling from sites of sympatry for LD mapping.

Comparative performance of Corymbia hybrids and parental species in subtropical Queensland and implications for breeding and deployment.

Eighty six full-sib Corymbia F1 hybrid families (crosses between C. torelliana and four spotted gum taxa: C. citriodora subsp. variegata, C. citriodora subsp. citriodora, C. henryi and C. maculata), were planted in six trials across six disparate sites in south-eastern Queensland to evaluate their productivity and determine their potential utility for plantation forestry. In each trial, the best-growing 20% of hybrid families grew significantly faster (P=0.05) than open-pollinated seedlots of the parent species Corymbia citriodora subsp. variegata, ranging from 107% to 181% and 127% to 287% of the height and diameter respectively. Relative performance of hybrid families growing on more than one site displayed consistency in ranking for growth across sites and analysis showed low genotype-by-environment interaction. Heritability estimates based on female and male parents across two sites at age six years for height and diameter at breast height, were high (0.62±0.28 to 0.64±0.35 and 0.31±0.21 to 0.69±0.37 respectively), and low to moderate (0.03±0.04 to 0.33±0.22) for stem straightness, branch size, incidence of ramicorns, and frost and disease resistance traits at ages one to three years. The proportion of dominance variance for height and diameter had reduced to zero by age six years. Based on these promising results, further breeding and pilot-scale family forestry and clonal forestry deployment is being undertaken. These results have also provided insights regarding the choice of a future hybrid breeding strategy.

Controlled pollination methods for creating Corymbia hybrids.

Inter-specific Corymbia hybrids are of increasing interest to plantation forestry, yet there is little knowledge of the most suitable controlled pollination methods for this genus. Inter-specific crosses were made between C. torelliana [CT(maternal parent)] and C. citriodora subsp. variegata (CCV), C. henryi (CH) and C. citriodora subsp. citriodora (CCC) using conventional pollination, one-stop pollination (OSP) and artificially-induced protogyny on yellow buds (AIP Y) pollination methods. Additional treatments included AIP on green buds (AIP G) and the use of exclusion bags for the OSP and AIP methods. Inter-specific hybrids (CT x CCV, CT x CH and CT x CCC) were successfully created using all three pollination methods. The AIP Y treatment provided the highest seed yields and achieved time savings of >41% over the conventional and OSP methods, resulting in up to five-fold increases in operator productivity. However, the AIP Y treatment also had the highest C. torelliana contamination levels (9.3–13.2%). The use of exclusion bags with the AIP method had minimal effect on contamination rates, indicating a high proportion of selfpollen contamination. Contamination rates varied between maternal parents, suggesting variation in selfcompatibility for C. torelliana individuals. AIP using semi-ripe green buds was not effective at reducing selfing and had low operator productivity. The AIP method is suitable for use in a large-scale hybrid breeding program for C. torelliana. When self-pollination effects are managed, it could greatly reduce the costs associated with the production of seed of elite family crosses for commercial forestry deployment.

Pollination methods, stigma receptivity and pollen tube growth in Eucalyptus argophloia

Eucalyptus argophloia Blakely (Western white gum) has shown potential as a commercial forestry timber species in marginal environments of north-eastern Australia. We measured early pollination success in Eucalyptus argophloia to compare pollination methods, determine the timing of stigma receptivity and compare fresh and stored pollen. Early pollination success was measured by counting pollen tubes in the style of E. argophloia 12 days after pollination. We compared the early pollination success of 1) Artificially Induced Protogyny (AIP), one-stop and three-stop methods of pollination; 2) flowers pollinated at 2 day intervals between 2 days before and 6 days after anthesis and 3) fresh pollen and pollen that had been stored for 9 months. Our results show significantly more pollen tubes from unpollinated AIP and AIP treatments than either the one-stop pollination or three-stop pollination treatments. This indicates that self-pollination occurs in the unpollinated AIP treatment. There was very little pollen tube growth in the one-stop method indicating that the three-stop method is the most suitable for this species. Stigma receptivity in E. argophloia commenced six days after anthesis and no pollen tube growth was observed prior to this. Fresh pollen resulted in pollen tube growth in the style whereas the stored pollen resulted in a total absence of pollen tube growth. We recommend that breeding programs incorporating E. argophloia as a female parent use the three-stop pollination method, and controlled pollination be carried out at least six days after anthesis using fresh pollen.

Determinacion de frecuencia de politraumatismo oseo (ptos) en mascotas caninas en el hospital animal El Dorado

Ante la falta de información acerca de la frecuencia, diagnóstico, tratamiento y primeros auxilios de la mascota canina politraumatizada en nuestro país, y la falta de medios para la praxis ortopédica, se propuso este estudio de tesis; cuyo titulo es: Determinación de frecuencia de politraumatismo óseo con diagnostico radiológico en mascotas caninas en el Hospital Animal El Dorado. Para lo cual se procedió a establecer los siguientes objetivos: la evaluación de la frecuencia de politraumatismos óseos (PTO) atendidos en la clínica veterinaria El Dorado con diagnostico radiológico, para esto se realizó dicho estudio en un periodo de 3 meses, en el cual se estudiaron expedientes y recopilación de datos, con este estudio de tesis se logró comprobar que la mayor afectación por fracturas la sufren los canes de raza criolla, seguidos por los Pastores Alemanes, así mismo se determinó que el manejo de las mascotas es determinante y predisponente a que una mascota sufra una fractura. Se identificó que los animales menores de 2 años sufren mayormente fracturas. Se concluyó que debido a los altos costos de la praxis, muchos veterinarios no atienden a sus pacientes y también debido a la misma razón muchos dueños de mascotas optan por la eutanasia o simplemente dejan que el tiempo repare las fracturas. Este estudio pretende ser una herramienta que permita al estudiante de Medicina Veterinaria tener un mayor entendimiento acerca de los politraumatismos. Para el profesional una referencia. Se concluyó que la raza y edad de las mascotas, así mismo, como la época del año (invierno) son factores determinantes en el surgimiento de Politraumatismo óseo, los cuales se localizan con mayor frecuencia en mascotas a nivel cubito/radio, fémur, tibia y peroné, la terapia mayormente utilizada fue la conservadora mediante el uso de férulas seguida de la quirúrgica con el uso de cerclaje.

Induccion del celo en vacas en anestro post parto mediante la administracion de sales minerales, vitaminas y masajes ovaricos. Realizada en el municipio de Rio Blanco, departamento de Matagalpa

El presente estudio se realizó con el objetivo de establecer la efectividad del tratamiento de Inducción al celo de vacas en anestro post parto por medio de la administración de sales minerales, vitaminas y masajes ovarios; dicho tratamiento fue llevado a cabo en el municipio de Río Blanco departamento de Matagalpa. Caracterizándose por una buena distribución de las lluvias la mayor parte del año. El dicho municipio se da una predominancia de una cultura de gran alta productividad ganadera, el tamaño requerido de la muestra de este trabajo de 20 hembras en periodo de anestro post parto mayor de 50 días, las cuales fueron seleccionadas al azar y evalua das clínicamente por palpación rectal por un profesional de la medicina veterinaria. Los datos fueron analizados mediante la prueba de chi –cuadrado habiendo obtenido un resultado significativo a un nivel de confianza del 95% entre los tratamientos. Con base en los resultados obtenidos posterior al tratamiento de la administración de sales minerales, vitamina y masaje ovárico se alcan zó una respuesta positiva del 80 % al celo, el 80% en tasa de preñez y en relación a la aparición del celo posteri or al tratamiento se alcanzo una media de 6.13 días que al comparar la efectividad del tratamiento de solo la aplicación de masaje ovárico se observo un buen resultado positivo, superando las expectativas. En comparación con las hembras tratadas con solo masaje ovárico se obtuvo una respuesta positiva al celo de un 60%, con un 30% de tasa de preñez y 7.33 días promedio en anestro posterior al tratamiento, por lo tanto elaboramos la hipótesis basándonos en el estadígrafo de Chi-cuadrado obteniendo un resultado significativo empleando un nivel de significancia del 0.05% por lo que atribuimos las diferencias de ambos tratamientos.