Anatomical and ultrastructural analyses of in vitro organogenesis from root explants of commercial passion fruit (Passiflora edulis Sims)

This study aimed to characterize the anatomical events and ultrastructural aspects of direct and indirect in vitro organogenesis in Passiflora edulis. Root explants were cultured on induction medium, supplemented with 4.44 mu M 6-benzyladenine. Roots at different stages of development were collected and processed for observation by light microscopy and scanning and transmission electron microscopy. Patterns of direct and indirect regeneration were observed in the explants. During direct organogenesis, the organogenic buds and nodules, formed from meristemoids, originated from the pericycle regions distant from the cut surface. Completely differentiated buds were observed after 20 days of culture. During indirect organogenesis, bud formation occurred via meristemoids at the periphery of the calli, which differentiated from the cortical region of the initial explant. Regardless of the regeneration pattern, the meristemoids had similar ultrastructural characteristics; however, differences were reported in the nuclear shape of the cells of the meristemoids formed directly and indirectly. This study provides important information for enhancing the understanding and characterization of the organogenic process in non-meristematic explants and provides information on the use of roots as explants in genetic transformation protocols for this important tropical species.

The fruit garden : a treatise intended to explain and illustrate the physiology of fruit trees ... illustrated with upwards of 150 figures /

Includes index.

The fruit garden : a treatise intended to explain and illustrate the physiology of fruit trees, the theory and practice of all operations connected with the propagation, transplanting, pruning and training of orchard and garden trees ... the laying out and arranging different kinds of orchards and gardens ... : illustrated with upwards of 150 figures ... /

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How to make a fruit garden; a practical and suggestive manual for the home garden,

Mode of access: Internet.

Trees, fruit and ornamental : text : essay /

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Deciduous fruit and tree nut research of the United States Department of Agriculture and cooperating agencies.

At head of title: A summary of current program and preliminary report of progress.

Report of the Royal commission investigating the fruit industry (and interrelated conditions) of the districts territorially known as the Okanagan, Kootenay, and Kettle river of the Province of British Columbia,

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The Fruit grower and farmer.

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Western Michigan : the land of fruit and fortune.

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California fruit and nut crops: acreage, production, utilization and value, 1949-1961; bloom, harvesting and marketing dates and principal producing counties.

Revises and updates California. Dept. of Agriculture. State publication 261.

Amateur fruit growing : A practical guide to the growing of fruit for home use and the market /

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"A survey of fruit and berry farms & farmers" and "Transient labor in the berry fields."

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Fruit tree and grape vine pruning, with an appendix on modern methods for pest and disease control.

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Growing fruit and vegetable crops.

Mode of access: Internet.

The orchard: including the management of wall and standard fruit trees, and the forcing pit; with selected lists and synonymes of the most choice varieties.

"Head gardener to His Grace the Duke of Buccleuch, at Dalkeith."