Rapid refolding of a proline-rich all-beta-sheet fibronectin type III module.

Fibronectin type III modules contain approximately 90 residues and are an extremely common building block of animal proteins. Despite containing a complex all-beta-sheet topology and eight prolines, the refolding of the 10th type III module of human fibronectin has been found to be very rapid, with native core packing, amide hydrogen bonding, and backbone conformation all recovered within 1 s at 5 degrees C. These observations indicate that this domain can overcome many structural characteristics often thought to slow the folding process.

The SNAP45 subunit of the small nuclear RNA (snRNA) activating protein complex is required for RNA polymerase II and III snRNA gene transcription and interacts with the TATA box binding protein.

The RNA polymerase II and III small nuclear RNA (snRNA) promoters contain a common basal promoter element, the proximal sequence element (PSE). The PSE binds a multisubunit complex we refer to as the snRNA activating protein complex (SNAPc). At least four polypeptides are visible in purified SNAPc preparations, which migrate with apparent molecular masses of 43, 45, 50, and 190 kDa on SDS/polyacrylamide gels. In addition, purified preparations of SNAPc contain variable amounts of TATA box binding protein (TBP). An important question is whether the PSEs of RNA polymerase II and III snRNA promoters recruit the exact same SNAP complex or slightly different versions of SNAPc, differing, for example, by the presence or absence of a subunit. To address this question, we are isolating cDNAs encoding different subunits of SNAPc. We have previously isolated the cDNA encoding the 43-kDa subunit SNAP43. We now report the isolation of the cDNA that encodes the p45 polypeptide. Antibodies directed against p45 retard the mobility of the SNAPc-PSE complex in an electrophoretic mobility shift assay, indicating that p45 is indeed part of SNAPc. We therefore refer to this protein as SNAP45. SNAP45 is exceptionally proline-rich, interacts strongly with TBP, and, like SNAP43, is required for both RNA polymerase II and III transcription of snRNA genes.

Fair Em, the miller's daughter of Manchester : with the love of William the Conqueror /

Imprint fictitious. Printed by the editor for private circulation. Cf. Jaggard, William. Shakespeare bibliography, 1911, page 552.

Letters of William III. and Louis XIV. and their ministers; illustrative of the domestic and foreign politics of England, from the peace of Ryswick to the accession of Philip V. of Spain, 1697 to 1700.

With appendix of treaties and documents.

The correspondence of King George the Third with Lord North from 1768 to 1783.

Running title: Letters to Lord North.

Lives of men of letters & science who flourished in the time of George III.

Vol. 2 has imprint: London, H. Colburn.

An account of the ceremonies observed in the coronations of the kings and queens of England : viz. King James II and his royal consort, King William III and Queen Mary, Queen Anne, King George I, and King George II and Queen Caroline.

In anticipation of the coronation of King George III.

Des hocherleuchteten sel. Johann Arndts, General-Superintendentens des Fürstenthums Lüneburg, Fünff geistreiche Bücher vom wahren Christenthum : welche handeln von heilsamer Busse, hertzlicher Reue und Leid über die Sünde, und wahrem Glauben, auch heiligem Leben und Wandel der Wahren Christen, auch wie ein wahrer Christ Sünde, Tod, Teufel, Hölle, Welt, Creutz und alle Trübsal, durch den Glauben, Gebet, Gedult, Gottes Wort und himmlischen Trost überwinden sol : und dasselbe alles in Christo Jesu : welchen noch beygefüget drey andere kleine Bücher, welche zu des sel. Johann Arndts wahren Christenthum gehörig, als I. Die Wiederholung und Verantwortung der Lehre vom wahren Christenthum, II. Zwey Sendschreiben an gute Freunde, die Bücher des wahren Christenthums betreffend, III. Bedencken über Tauleri Teutsche Theologie, wie hoch solche zu halten ...

Includes index.

Original ancedotes of Frederick the Great, king of Prussia, and of his family, his court, his ministers, his academies, and his literary friends.

Mode of access: Internet.

Das Leben Kaiser Friedrichs III /

Spine title: Kaiser Friedrich.

Charakter-Züge und historische Fragmente aus dem Leben des Königs von Preussen Friedrich Wilhelm III.

Mode of access: Internet.

Wilhelms I. Briefe an seinen Vater König Friedrich Wilhelm III. (1827-1839)

Mode of access: Internet.

Statement of Prince Frederick of Schleswig Holstein Noer. The crown of Denmark disposed of by a religious minister through a fraudulent treaty /

Mode of access: Internet.

König Friedrich Wilhelm III., sein anteil an der konvention von Tauroggen und an der reform von 1807-1812.

"Sonderabzug aus den Forschungen zur brandenburgischen und preussischen geschichte. Bd. XVIII."

The story of the great war ...

Another issue in 8 vols. has title: The story of the great war, the complete historical records of events to date. Illustrated with drawings, maps and photographs by Francis J. Reynolds ... Allen L. Churchill ... Francis Trevelyan Miller ...