AMERICAN TRYPANOSOMIASIS (CHAGAS') DISEASE - A STUDY OF FETO-MATERNAL MORBIDITY AND MORTALITY

The possibility of a relationship between American Trypanosomiasis (Chagas') disease and pregnancy outcome was analyzed measuring feto-maternal morbidity and mortality in a sample of 604 pregnant women and their offspring seen at the Hospital Universitario de Maternidad y Neonatologia in Cordoba, Argentina during 1979.^ A cross-sectional, "case-comparison" investigation was employed to determine the degree of risk between having a reactive chagasic serologic test and a negative pregnancy outcome as determined by abortion, stillbirth, and infant death prior to one week of age. Patients were selected using a dichotomous, 0-1 scale with either the presence or the absence of a reactive Machado-Guerreiro complement fixation serologic blood test result.^ The data obtained were analyzed using appropriate statistical techniques for measuring the comparisons between the case and control groups under various demographic and socioeconomic variables such as, age, marital status, educational attainment, and residence. Similarly, additional biological variables of birth order, maternal and fetal complications, and prematurity were examined.^ From the analysis of the data obtained in this investigation, no definite conclusions can be reached regarding the risk of having an unsuccessful pregnancy outcome in the presence of a reactive serologic finding because the study design was a cross-sectional one and the number of events were too few for an adequate analysis. Notwithstanding these limitations, the results obtained, after statistical adjustments were employed, demonstrated that women with a reactive test result were older, were of a higher parity, and were less educated. Marital status and residence were not significant variables. The risk of pregnancy wastage, however, was almost twice as frequent in the reactive group as in the non-reactive group of women. Statistically significant differences in maternal morbidity involved two complications, polyhydramnios and varicosities of the lower extremities and vulva; while in the newborn, infection was higher in infants whose mothers exhibited a reactive serologic test result.^ In summary, what this research study has shown is the need for engaging in a larger, longitudinal study for an in-depth exploration of feto-maternal morbidity and mortality--an investigation that would corraborate or refute the findings of this study.^

Determinación de la actividad de la óxido nítrico sintetasa endotelial y del factor NF-kB, en la piel del paciente con pseudoangiomatosis eruptiva : informe preliminar

La pseudoangiomatosis eruptiva se caracteriza por la aparición brusca de múltiples pápulas eritematosas, asintomáticas, rodeadas de un halo blanquecino, con remisión espontánea. Histológicamente se observa dilatación vascular con escaso infiltrado inflamatorio. Su etiología permanece incierta a pesar de ser relacionada con virus o picaduras de insectos. Basados en el compromiso vascular, el objetivo del trabajo fue investigar la actividad de la enzima endotelial oxido nítrico sintetasa (eNOS) y la expresión del factor NF-kB por inmunohistoquimica en un intento de esclarecer su patogenia. Material y métodos: Se estudiaron diez pacientes con diagnóstico clínico de pseudoangiomatosis eruptiva (PAE) que presentaron la dermatosis en forma epidémica. Se realizaron biopsias teñidas con Hematoxilina-Eosina y Tricrómico de Masson. Se efectuó estudio virológico de los pacientes Nª 4, 9 y 10 mediante determinaciones serológicas para echovirus, enterovirus, citomegalovirus, parvovirus B19 y hepatitis A, B y C. En cinco pacientes se obtuvo material para determinación de eNOS y NF-kB. Resultados: Todos los pacientes, 5 hombres y 5 mujeres presentaron pápulas eritematosas rodeadas por un halo blanquecino, especialmente en las extremidades, alrededor de las rodillas. Histológicamente mostraron vasos dilatados y células endoteliales prominentes con un infiltrado discreto perivascular. Todos los estudios serológicos fueron negativos. La actividad de eNOS fue significativamente menor comparada con la piel normal (p= 0,002) y la expresión de NF- ĸB fue fuertemente positiva en los vasos de la dermis papilar y reticular. Conclusiones: Todos los pacientes fueron afectados en verano, por lo que la picadura del mosquito debe ser considerada como un factor etiológico. La baja expresión de eNOS está relacionada con la vasodilatación y la expresión aumentada de NF-ĸB confirma que el proceso es de tipo inflamatorio.

Hipertricosis adquirida y localizada en fascitis eosinofílica

La fascitis eosinofílica, se caracteriza por cambios cutáneos símil- esclerodermia y eosinofília periférica. Describimos un paciente de 54 años, de sexo masculino con diagnóstico de fascitis eosinofílica y seguimiento de 44 meses. Presentaba tumefacción, engrosamiento de la piel de antebrazos, piernas y flancos de abdomen, signo del surco y pérdida del pelo de antebrazos y piernas. El paciente fue tratado con glucocorticoides e hidroxicloroquina con evolución favorable. Concomitante a la remisión de los signos cutáneos observamos una hipertricosis de las zonas previamente afectadas. Comunicamos esta observación inusual en fascitis eosinofílica, secundaria a la mejoría del proceso inflamatorio cutáneo.

Palynology on sediment profile Tso Kar in Ladakh

Palynological investigation of a 410 cm long core section from Tso Kar (33°10'N, 78°E, 4527 m a.s.l.), an alpine lake situated in the arid Ladakh area of NW India at the limit of the present-day Indian summer monsoon, was performed in order to reconstruct post-glacial regional vegetation and climate dynamics. The area was covered with alpine desert vegetation from ca. 15.2 to 14 kyr BP (1 kyr=1000 cal. years), reflecting dry and cold conditions. High influx values of long-distance transported Pinus sylvestris type pollen suggest prevailing air flow from the west and northwest. The spread of alpine meadow communities and local aquatic vegetation is a weak sign of climate amelioration after ca. 14 kyr BP. Pollen data (e.g. influx values of Pinus roxburghii type and Quercus) suggest that this was due to a strengthening of the summer monsoon and the reduced activity of westerly winds. The further spread of Artemisia and species-rich meadows occurred in response to improved moisture conditions between ca. 12.9 and 12.5 kyr BP. The subsequent change towards drier desert-steppe vegetation likely indicates more frequent westerly disturbances and associated snowfalls, which favoured the persistence of alpine meadows on edaphically moist sites. The spread of Chenopodiaceae-dominated vegetation associated with an extremely weak monsoon occurred at ca. 12.2-11.8 kyr BP during the Younger Dryas interstadial. A major increase in humidity is inferred from the development of Artemisia-dominated steppe and wet alpine meadows with Gentianaceae after the late glacial/early Holocene transition in response to the strengthening of the summer monsoon. Monsoonal influence reached maximum activity in the Tso Kar region between ca. 10.9 and 9.2 kyr BP. The subsequent development of the alpine meadow, steppe and desert-steppe vegetation points to a moderate reduction in the moisture supply, which can be linked to the weaker summer monsoon and the accompanying enhancement of the winter westerly flow from ca. 9.2 to 4.8 kyr BP. The highest water levels of Tso Kar around 8 kyr BP probably reflect combined effect of both monsoonal and westerly influence in the region. An abrupt shift towards aridity in the Tso Kar region occurred after ca. 4.8 kyr BP, as evidenced by an expansion of Chenopodiaceae-dominated desert-steppe. Low pollen influx values registered ca. 2.8-1.3 kyr BP suggest scarce vegetation cover and unfavourable growing conditions likely associated with a further weakening of the Indian Monsoon.

Production fragmentation, upstreamness, and value-added : evidence from factory Asia 1990-2005

We exploit the recent release of the 2005 Asian Input-Output Matrix to dress a picture of the geographic fragmentation of value added in Factory Asia from 1990 to 2005. We document 3 stylized facts. The first is that the average share of foreign value added embedded in production rose by about 7 percentage points between 1990 and 2005, from 9% to 16%. The second is that, contrary to popular belief, China's production embeds a smaller share of foreign value added than other Factory Asia countries'. Between 1990 and 2005 among Factory Asia countries China grew most after Japan as a source of value added to other countries' production. Third, country-industries at the upstream and downstream extremities of the supply chain embed a smaller share of foreign value added than those with intermediate levels of upstreamness.

Epidemiology of injuries in First Division Spanish football

Abstract The aim was to examine the injuries sustained by Spanish football players in the First Division and to compare injury-related variables in the context of both competition and training. The injury data were prospectively collected from 16 teams (427 players) using a specific web-based survey during the 2008/2009 season. A total of 1293 injuries were identified (145 were recurring injuries). The overall injury incidence was 5.65 injuries per 1000 h of exposure. Injuries were much more common during competition than during training (43.53 vs. 3.55 injuries per 1000 h of exposure, P menor que 0.05). Most of the injuries (89.6%) involved the lower extremities, and overuse (65.7%) was the main cause. Muscle and tendon injuries were the most common types of injury (53.8%) among the players. The incidence of training injuries was greater during the pre-season and tended to decrease throughout the season, while the incidence of competition injuries increased throughout the season (all P menor que 0.05). In conclusion, the results of this study suggest the need for injury prevention protocols in the First Division of the Spanish Football League to reduce the number of overuse injuries in the muscles and tendons in the lower extremities. In addition, special attention should be paid during the pre-season and the competitive phase II (the last four months of the season) in order to prevent training and competition injuries, respectively.

Use of the energy-release rate in a 2 scale damage analysis of a bonded joint

Stress singularities appear at the extremities of an adhesive bond. They can produce a damage mechanism that we assimilate in this Note to a crack. The energy release rate permits to characterize its evolution. But a very refined mesh would be necessary for a real structure. Using an asymptotic method based on the small thickness of the bond a limit model with a different local behaviour is suggested. It leads to an approximation of the energy release rate

Análisis de la incidencia lesional en el fútbol profesional español en la temporada 2008-2009

Se ha descrito en la literatura como el fútbol es un deporte en cuya práctica existe una alta incidencia de lesión. Sin embargo, en España, hasta el momento no se había llevado a cabo ningún estudio que analizara la incidencia de lesión de los jugadores de fútbol profesional. Para poder prevenir lesiones, en primer lugar debemos de identificar la magnitud del problema, así como conocer aquellos factores que pueden influir sobre la producción de lesiones, Éste es principal objetivo de nuestro estudio para, a partir de él, poder diseñar e introducir en la práctica deportiva medidas preventivas con el objetivo de disminuir la incidencia de lesión en el fútbol. Hay que considerar que nos encontramos ante un estudio descriptivo, el cual se caracteriza, por no tener una hipótesis previa. En él nos planteamos como principal objetivo el conocer la influencia de los diferentes factores que influyen en la posibilidad de sufrir una lesión en el fútbol, además de realizar un análisis que describiera las lesiones sufridas, así como sus periodos de recuperación, en el fútbol profesional español, En esta investigación, participaron 27 equipos pertenecientes a la primera y segunda división española (728 jugadores), registrándose durante la temporada 2008- 2009 un total de 2184 lesiones. Para el registro de las lesiones, se utilizó el cuestionario REINLE cumplimentado de forma confidencial por miembros del equipo técnico de los clubes colaboradores, a través de la base de datos LEFUTPRO con acceso vía internet. Como dato general, encontramos que en la práctica del fútbol profesional existe una elevada incidencia de lesión con 8,92 les.*1000h. de exposición, siendo ésta mayor en competición (41,33 les.*1000h.) que en entrenamiento (6,02 les.*1000h.), valores muy acordes a los encontrados en la literatura científica especifica. Se registraron un promedio de 81 lesiones que causaron baja por equipo y temporada, provocando una ausencia media por lesión de 11,3 días. De estas lesiones, el 11,9% de ellas fueron de carácter recidivante, las cuales causaron periodos de baja superiores a las lesiones iníciales, alcanzando un valor promedio de 17,9 días de baja por lesión. El 84,4% las lesiones que se registraron, se localizaron en la extremidad inferior, siendo el muslo, con un 30,5% de todas las lesiones, la región corporal más afectada. A su vez, dentro del muslo, el 53% de las lesiones se localizaron en la región posterior, dentro de la cual, en el 73% de los casos la lesión afectó al bíceps femoral. Además, se encontró una mayor frecuencia de lesión en la pierna dominante (54,4% de las todas las lesiones) frente a la no dominante (36,5% de las todas las lesiones). Desde el punto de vista de la tipología de la lesión, las lesiones de carácter muscular con un 49,1% de los casos, fueron las más frecuentes, seguidas de las lesiones ligamentosas con un 15,1% de los casos. Los mecanismos de producción de lesiones en el jugador sin contacto, fueron más frecuentes (69,1% de todos los casos), que aquellos mecanismos de lesión en los que si hubo contacto (30,9%). En relación al momento de producción de lesión a lo largo de la temporada, se encontró que las lesiones sufridas en entrenamiento fueron progresivamente menos frecuentes con el transcurso de la temporada, mientras que en la competición se fueron incrementando, siendo los últimos meses de la temporada cuando se registraron la mayor parte de las lesiones. A partir de este estudio, proponemos ampliar la investigación en esta línea, concretando algunos de los aspectos que hemos podido identificar a través de nuestros resultados, y que en un futuro puedan tener alguna aplicación práctica en el objetivo común de reducir la incidencia de lesión existente en la práctica deportiva del fútbol. ABSTRACT Football has been described in literature as a sport in which there is a high incidence of injuries. Nevertheless, in Spain, to date, not a single study analyzing the incidence of lesions in professional football players has been carried out. In order to prevent lesions, in the first place we must identify the magnitude of the problem, as well as discover those factors that may influence on the production of lesions, being the latter the main objective of our study, and hence, starting from this point, be able to design and introduce in sports practice preventive measures with the aim of decreasing the incidence of lesions. We must take into consideration that we are before a study characterized by the fact of not having a previous hypothesis. In the present study, our main areas of focus is finding out the different factors that have influence on the possibility of suffering a lesion in this sports practice, as well as carrying out an analysis that describes the lesions suffered in professional football, as well as the necessary recovery periods. 27 teams (728 players), belonging to Spain’s First and Second Football (soccer) Divisions took part in this research. During the 2008-2009 season, a total of 2,184 lesions were registered. To register the lesions, a REINLE questionnaire, of the LEFUTPRO database was used, with access via internet. Each of the participating teams was given a password in a totally confidential manner. In the first place, we found that in the practice of professional football there exists a high incidence of lesions, with 8.92 lesions per 1000 h. of exposure. We found an increase in lesions during competitions (41.33 lesions per 1000 h.) with respect to those occurring during training (6.02 lesions per 1000 h.); these values being in accordance with those found in specific scientific literature. An average of 81 lesions that implied sick leaves were registered per team and season, causing an average of 11.3 days of absence per lesion. Of these, 11.9% of all the lesions studied were recurrent, causing longer sick leave periods than the ones due to the initial lesions and reaching an average of 17.9 days of sick leave per lesion. 84.4% of the lesions registered were located in the inferior extremities, the thigh being the anatomical region, with 30.5% of all the lesions, the most affected body region. In turn, 53% of the lesions to the thigh occurred in the posterior area, and within the latter, in 73% of the cases, the lesion affected the femoral biceps. In addition, we found a greater frequency of lesions in the dominant leg (54.4% of all the lesions), in contrast with the non dominant leg (36.5% of all the lesions). From the point of view of lesion type the most frequent lesions were muscular (49.1% of all cases), followed by ligament lesions (15.1%). Most of the lesions were produced during moments in which the players were not in physical contact at the moment of suffering the injury (69.1%), in comparison with those produced when there was physical contact (30.9%). With relation to the instance in which the injuries were produced along the season, we found that those lesions suffered during training were progressively less frequent as the season wore on, whilst those suffered during competitions gradually incremented, being the last months of the season when most of the injuries were registered. From the research of this study, we propose the need to carry out more research in this same line, focalizing on some more concrete aspects, which, through our results, we have been able to realize that would be interesting to study in greater depth, and that in the future, our results may have a practical application, helping reduce the incidence of injuries that exist in this sport practice.

Retardation of skeletal development and cervical abnormalities in transgenic mice expressing a dominant-negative retinoic acid receptor in chondrogenic cells

Skeletal formation is a fundamental element of body patterning and is strictly regulated both temporally and spatially by a variety of molecules. Among these, retinoic acid (RA) has been shown to be involved in normal skeletal development. However, its pleiotropic effects have caused difficulty in identifying its crucial target cells and molecular mechanisms for each effect. Development of cartilage primordia is an important process in defining the skeletal structures. To address the role of RA in skeletal formation, we have generated mice expressing a dominant-negative retinoic acid receptor (RAR) in chondrogenic cells by using the type II collagen α1 promoter, and we have analyzed their phenotypes. These mice exhibited small cartilage primordia during development and retarded skeletal formation in both embryonic and postnatal periods. They also showed selective degeneration in their cervical vertebrae combined with homeotic transformations, but not in their extremities. The cervical phenotypes are reminiscent of phenotypes involving homeobox genes. We found that the expression of Hoxa-4 was indeed reduced in the cartilage primordia of cervical vertebrae of embryonic day 12.5 embryos. These observations demonstrate that endogenous RA acts directly on chondrogenic cells to promote skeletal growth in both embryonic and growing periods, and it regulates the proper formation of cervical vertebrae. Furthermore, RA apparently specifies the identities of the cervical vertebrae through the regulation of homeobox genes in the chondrogenic cells. Great similarities of the phenotypes between our mice and reported RAR knockout mice revealed that chondrogenic cells are a principal RA target during complex cascades of skeletal development.

Mechanical separation of the complementary strands of DNA

We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage λ DNA. The 3′ and 5′ extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10–15 pN. The separation force signal is shown to be related to the local GC vs. AT content along the molecule. Variations of this content on a typical scale of 100–500 bases are presently detected.

Quantitative, chemically specific imaging of selenium transformation in plants

Quantitative, chemically specific images of biological systems would be invaluable in unraveling the bioinorganic chemistry of biological tissues. Here we report the spatial distribution and chemical forms of selenium in Astragalus bisulcatus (two-grooved poison or milk vetch), a plant capable of accumulating up to 0.65% of its shoot dry biomass as Se in its natural habitat. By selectively tuning incident x-ray energies close to the Se K-absorption edge, we have collected quantitative, 100-μm-resolution images of the spatial distribution, concentration, and chemical form of Se in intact root and shoot tissues. To our knowledge, this is the first report of quantitative concentration-imaging of specific chemical forms. Plants exposed to 5 μM selenate for 28 days contained predominantly selenate in the mature leaf tissue at a concentration of 0.3–0.6 mM, whereas the young leaves and the roots contained organoselenium almost exclusively, indicating that the ability to biotransform selenate is either inducible or developmentally specific. While the concentration of organoselenium in the majority of the root tissue was much lower than that of the youngest leaves (0.2–0.3 compared with 3–4 mM), isolated areas on the extremities of the roots contained concentrations of organoselenium an order of magnitude greater than the rest of the root. These imaging results were corroborated by spatially resolved x-ray absorption near-edge spectra collected from selected 100 × 100 μm2 regions of the same tissues.

Force and kinetic barriers to unzipping of the DNA double helix

A theory of the unzipping of double-stranded DNA is presented and is compared to recent micromanipulation experiments. It is shown that the interactions that stabilize the double helix and the elastic rigidity of single strands simply determine the sequence-dependent ≈12-pN force threshold for DNA strand separation. Using a semimicroscopic model of the binding between nucleotide strands, we show that the greater rigidity of the strands when formed into double-stranded DNA, relative to that of isolated strands, gives rise to a potential barrier to unzipping. The effects of this barrier are derived analytically. The force to keep the extremities of the molecule at a fixed distance, the kinetic rates for strand unpairing at fixed applied force, and the rupture force as a function of loading rate are calculated. The dependence of the kinetics and of the rupture force on molecule length is also analyzed.

Amplification of the full-length hepatitis A virus genome by long reverse transcription-PCR and transcription of infectious RNA directly from the amplicon.

The genetic study of RNA viruses is greatly facilitated by the availability of infectious cDNA clones. However, their construction has often been difficult. While exploring ways to simplify the construction of infectious clones, we have successfully modified and applied the newly described technique of "long PCR" to the synthesis of a full-length DNA amplicon from the RNA of a cytopathogenic mutant (HM 175/24a) of the hepatitis A virus (HAV). Primers were synthesized to match the two extremities of the HAV genome. The antisense primer, homologous to the 3' end, was used in both the reverse transcription (RT) and the PCR steps. With these primers we reproducibly obtained a full-length amplicon of approximately 7.5 kb. Further, since we engineered a T7 promoter in the sense primer, RNA could be transcribed directly from the amplicon with T7 RNA polymerase. Following transfection of cultured fetal rhesus kidney cells with the transcription mixture containing both the HAV cDNA and the transcribed RNA, replicating HAV was detected by immunofluorescence microscopy and, following passage to other cell cultures, by focus formation. The recovered virus displayed the cytopathic effect and large plaque phenotype typical of the original virus; this result highlights the fidelity of the modified long reverse transcription-PCR procedure and demonstrates the potential of this method for providing cDNAs of viral genomes and simplifying the construction of infectious clones.