Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization.

Virus-like particles (VLPs) are non-infectious self-assembling nanoparticles, useful in medicine and nanotechnology. Their repetitive molecularly-defined architecture is attractive for engineering multivalency, notably for vaccination. However, decorating VLPs with target-antigens by genetic fusion or chemical modification is time-consuming and often leads to capsid misassembly or antigen misfolding, hindering generation of protective immunity. Here we establish a platform for irreversibly decorating VLPs simply by mixing with protein antigen. SpyCatcher is a genetically-encoded protein designed to spontaneously form a covalent bond to its peptide-partner SpyTag. We expressed in E. coli VLPs from the bacteriophage AP205 genetically fused to SpyCatcher. We demonstrated quantitative covalent coupling to SpyCatcher-VLPs after mixing with SpyTag-linked to malaria antigens, including CIDR and Pfs25. In addition, we showed coupling to the VLPs for peptides relevant to cancer from epidermal growth factor receptor and telomerase. Injecting SpyCatcher-VLPs decorated with a malarial antigen efficiently induced antibody responses after only a single immunization. This simple, efficient and modular decoration of nanoparticles should accelerate vaccine development, as well as other applications of nanoparticle devices.

The laws of contrast and colour and their application to the arts of painting, decoration of buildings, mosaic work ... /

Mode of access: Internet.

House decoration : comprising whitewashing, paperhanging, painting, etc. ; with numerous engravings and diagrams /

First edition ... 1894. Reprinted ... 1908.

Modern bookbinding, their design and decoration.

Mode of access: Internet.

Art industry, furniture, upholstery, and house decoration.

Mode of access: Internet.

Bowl with Green, Yellow, and Brown Splashed Decoration

Pottery, Iran, Samanid; 2 7/8 in.x 10 15/64 in.; earthenware; white slip, incised and splashed with polychrome glazes under transparent glaze (sgraffito ware)

Painting and decorating : a journal treating of house, sign, fresco, car, and carriage painting and of wall paper and decoration.

Editors: Oct. 1885--Dec. 1886, A. A. Kelly.--Jan. 1887-Dec. 1888, Frederick Maire (with A. A. Kelly, Jan.-May, 1887)--Jan. 1889-Jan.? 1893, A. S. Jennings.

Holiday selections for readings and recitations, specially adapted to Christmas, New Year, Valentine's day, Washington's birthday, Easter, Arbor day, Decoration day, Fourth of July, and Thanksgiving,

Mode of access: Internet.

Patriotic recitations and readings, containing a large number of the most effective, eloquent, instructive and brilliant selections for Fourth of July, Decoration, Arbor and Labor Day, Washington and Lincoln's birthdays, and all other holiday celebrations.

Mode of access: Internet.

Furniture and interior decoration of the Italian Renaissance /

Includes bibliographical references.

The apartments of the house : their arrangement, furnishing and decoration /

Mode of access: Internet.

Decoration of the school and home /

Mode of access: Internet.