224 resultados para DUPIN HYPERSURFACES


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Cover-title: L'épée de Napoléon. Mémoire à consulter, par Me. Patorni, suivi des consultations de Mes. Odilon-Barrot, Pailliet, Ph. Dupin ... [e.a.] Avec la réponse de Joseph-Napoléon Bonaparte.

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Mode of access: Internet.

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Ascribed also to M.J. Pain. cf. Wicks, C.B., The Parisian stage, I.

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I. De la stabilité des corps flottants -- II. Du tracé des routes Isolées -- III. Sur le tracé des routes, dans les débiais et les rembials -- IV. Sur les routes sulvies par la lumiére et par les corps élastiques, en général, dans les phénoménes de la réflexion et de la réfraction -- V. Examen théorique de la structure des vaisseaux angials.

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Mode of access: Internet.

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The efficient transport of micron-sized beads into cells, via a non-endocytosis mediated mechanism, has only recently been described. As such there is considerable scope for optimization and exploitation of this procedure to enable imaging and sensing applications to be realized. Herein, we report the design, synthesis and characterization of fluorescent microsphere-based cellular delivery agents that can also carry biological cargoes. These core-shell polymer microspheres possess two distinct chemical environments; the core is hydrophobic and can be labeled with fluorescent dye, to permit visual tracking of the microsphere during and after cellular delivery, whilst the outer shell renders the external surfaces of the microspheres hydrophilic, thus facilitating both bioconjugation and cellular compatibility. Cross-linked core particles were prepared in a dispersion polymerization reaction employing styrene, divinylbenzene and a thiol-functionalized co-monomer. These core particles were then shelled in a seeded emulsion polymerization reaction, employing styrene, divinylbenzene and methacrylic acid, to generate orthogonally functionalized core-shell microspheres which were internally labeled via the core thiol moieties through reaction with a thiol reactive dye (DY630-maleimide). Following internal labeling, bioconjugation of green fluorescent protein (GFP) to their carboxyl-functionalized surfaces was successfully accomplished using standard coupling protocols. The resultant dual-labeled microspheres were visualized by both of the fully resolvable fluorescence emissions of their cores (DY630) and shells (GFP). In vitro cellular uptake of these microspheres by HeLa cells was demonstrated conventionally by fluorescence-based flow cytometry, whilst MTT assays demonstrated that 92% of HeLa cells remained viable after uptake. Due to their size and surface functionalities, these far-red-labeled microspheres are ideal candidates for in vitro, cellular delivery of proteins, as described in the accompanying paper.

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DUE TO COPYRIGHT RESTRICTIONS ONLY AVAILABLE FOR CONSULTATION AT ASTON UNIVERSITY LIBRARY AND INFORMATION SERVICES WITH PRIOR ARRANGEMENT

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A short paper giving some examples of smooth hypersurfaces M of degree n+1 in complex projective n-space that are defined by real polynomial equations and whose real slice contains a component diffeomorphic to an n-1 torus, which is then special Lagrangian with respect to the Calabi-Yau metric on M.

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Having recently celebrated the bicentenary of Edgar Allan Poe’s birth, his tales still remain an invaluable source of ongoing interpretation. The first tale that conforms his detective trilogy, “The Murders in the Rue Morgue”, has been analysed from multiple perpectives, especially taking into consideration the discourse of race as a main focus of attention. Nonetheless, detective Auguste Dupin’s references to Georges Cuvier, an important preDarwinian French naturalist and zoologist, have often been unnoticed. This article aims at identifying pre-Darwinian concepts about selection and evolution in Poe’s tale so as to facilitate readings of the tale in relation to Darwinism and evolutionary theories