The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms

The aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases.

Geostatistics and remote sensing methods in the classification of images of areas cultivated with citrus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Candidatus Liberibacter asiaticus Titers in Citrus and Acquisition Rates by Diaphorina citri Are Decreased by Higher Temperature

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Healing Actions of Essential Oils from Citrus aurantium and d-Limonene in the Gastric Mucosa: The Roles of VEGF, PCNA, and COX-2 in Cell Proliferation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The effect of a minor constituent of essential oil from Citrus aurantium: The role of beta-myrcene in preventing peptic ulcer disease

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The influence of citrus rootstocks on the relationship between the mite Brevipalpus phoenicis and citrus leprosis disease

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparative proteomic analysis reveals that T3SS, Tfp, and xanthan gum are key factors in initial stages of Citrus sinensis infection by Xanthomonas citri subsp citri

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Occurrence of Helicoverpa armigera (Hübner, 1808) on citrus in the state of Sao Paulo, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Citrus pulp and enzyme complex for growing and finishing pigs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estrutura de populações e inoculações recíprocas de Xylella fastidiosa subsp. pauca com ocorrência em cultivos vizinhos de Citrus sinensis e Coffea arabica sob condições do estado de São Paulo

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Atividade larvicida dos óleos essenciais de Syzygium aromaticum e Citrus sinensis em populações de Aedes aegypti

Pós-graduação em Biociências - FCLAS

Technical and economic evaluation of different types of control of Brevipalpus phoenicis (Acari: Tenuipalpidae) in citrus leprosies management

The objective of this work was to evaluate the effect of acaricide applications and pruning of symptomatic branches in citrus leprosis management in Brazil. It was conducted in an orange plantation of the 'Pera' variety, grafted onto the 'Cleopatra' tangerine, in two seasons (2006-2007 and 2007-2008). The experimental design was randomized blocks in a factorial scheme consisting of the following factors: (A) acaricide, in three levels: spirodiclofen and cyhexatin applied in rotation, lime sulphur; no acaricides; (B) pruning to remove branches that showed symptoms of leprosis, with two levels: with pruning, without pruning. We carried out periodic assessments of Brevipalpus phoenicis (Geijskes) populations (vector of the leprosis virus), leprosis incidence and severity, fruit yield, and the economic feasibility of the applied strategies. Based on the results, we concluded that spirodiclofen and cyhexatin were more effective than lime sulphur in B. phoenicis control. Control with lime sulphur required more applications than spirodiclofen and cyhexatin in rotation, making it more expensive. Pruning of symptomatic branches used in isolation was not sufficiently effective to control leprosis and significantly increased control costs. Profits were higher when the control involved sprayings of spirodiclofen and cyhexatin in alternation, with or without pruning.

Avaliação da atividade ansiolítica e antidepressiva do óleo essencial da Citrus aurantium L. administrado por via inalatória em camundongos

A investigação de plantas medicinais é indispensável na busca de novas substâncias com potencial atividade ansiolítica e antidepressiva, visto que a ansiedade e depressão estão entre os distúrbios do sistema nervoso central (SNC) mais relevantes dentre os problemas de saúde mundial. Tais plantas são fontes de óleos essenciais (OE) que frequentemente são utilizados na aromaterapia, pois fornecem substâncias voláteis capazes de curar e prevenir doenças por meio da inalação. Esta prática já se mostrou efetiva sobre o SNC, trazendo benefícios terapêuticos a pacientes que não respondiam bem aos tratamentos convencionais. Uma espécie vegetal cujo OE possui atividade sobre o SNC é o Citrus aurantium L. (laranja amarga), espécie do gênero que mostrou atividade ansiolítica quando utilizado na aromatização de ambiente na sala de espera de um consultório dentário. Aqui avaliamos o potencial efeito ansiolítico e antidepressivo do OE de Citrus aurantium L por via inalatória em camundongos, por meio da resposta comportamental em modelos experimentais de ansiedade, o Labirinto em Cruz Elevado, e de depressão, o Teste do Nado Forçado. Com esta avaliação, o OE não foi efetivo por via inalatória em nenhuma das concentrações testadas (0,5%, 1,0% e 2,5%), tanto para ansiedade quanto para depressão. No entanto, este resultado não exclui a possibilidade de obtenção de tais efeitos se alterada a concentração de OE

Transcriptoma da interação de tangerina satsuma (Citrus unshiu) e laranja doce Hamlin (Citrus sinensis) infectadas com Xanthomonas citri subsp. citri, agente causal do cancro cítrico

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Citrus greening disease investigation using micro synchrotron radiation X-ray fluorescence in association with soft independent modelling of class analogy (SIMCA)

This study describes the use of micro synchrotron radiation X-ray fluorescence (µSR-XRF) to investigate citrus greening disease in sweet orange (Citrus sinensis) plants. An experiment using healthy plants as control and plants of the same variety infected with Candidatus Liberibacter asiaticus (CLas) was performed to verify variations of the mineral composition of citrus leaves. A µSR-XRF system using the D09B X-ray fluorescence beam line at the Brazilian Synchrotron Light Source (LNLS, Campinas, São Paulo State) was employed for this purpose. The data were analyzed using a chemometric tool called soft independent modelling of class analogy (SIMCA). The promising results from SIMCA models reinforce the evidence that plants infected by citrus greening (both asymptomatic and symptomatic) undergo alterations in their micro- and macronutrient compositions.