Mycotoxins and abnormal embryonic development

This chapter reviews studies on the effects of mycotoxins on embryonic and fetal development, especially those toxins that are global food and feed contaminants. The toxins discussed include aflatoxin produced by Aspergillus flavus and A. parasiticus, ochratoxin which is produced by Aspergillus species particularly A. ochraceus as well as Penicillium verrucosum, ergot alkaloids produced by Claviceps spp., and the Fusarium toxins (fumonisins, deoxynivalenol [vomitoxin], and zearalenone). These toxins have been shown to be teratogenic and/or embryotoxic in different animal bioassays. The implications of toxicity on embryogenesis, and the progress of research on these mycotoxins, are also examined.

Nutrition of females during the peri-conceptional period and effects on foetal programming and health of offspring

The period around the time of conception is one characterised by considerable cytological and molecular restructuring as ovulation occurs, the oocyte is fertilised and the embryonic developmental programme begins. The intrinsic processes regulating peri-conceptional progression are supplemented by environmental factors, which contribute important metabolic information that influences several aspects of the developmental programme. Indeed, there is growing evidence from different mammalian animal models, reviewed here, that the peri-conceptional environment mediated through maternal nutrition can modify development throughout gestation and affect the physiological and metabolic health of adult offspring. The concept that adult disease risk may owe its origin to the quality of peri-conceptional maternal nutrition is one, which merits further research for mechanistic understanding and devising preventive strategies. © 2012 Elsevier B.V.

Una reflexión prospectiva sobre técnicas sostenibles para controlar la reproducción en mamíferos domésticos

El control de la reproducción de los animales de granja fue una de las herramientas esenciales en la domesticación. Esto es aún de gran interés para la mejora genética, el ajuste de la producción a la disponibilidad de alimentos y del mercado y finalmente, para la reducción de los períodos improductivos. La detección del comportamiento del estro, la sincronización de las concepciones y el incremento de la potencialidad de difusión de los padres de alta genética son tres objetivos comunes a la producción de todas las especies. Los variados sistemas reproductivos entre los diversos sistemas de producción difieren a causa de las propiedades intrínsecas de las especies y de los diferentes grados de intensificación de estos sistemas. Tres tendencias claras son el continuo incremento de la productividad por medio de las mejoras de la eficiencia reproductiva, el desarrollo de técnicas nuevas y sostenibles sin el uso de hormonas y el continuo desarrollo de la inseminación artificial y de las biotécnicas reproductivas. Las futuras áreas de inversión en investigación podrían ser: las bases fisiológicas y etológicas de las interrelaciones socio-sexuales entre animales, el control genético de parámetros reproductivos, el incremento en la eficiencia de biotecnologías clásicas y nuevas y la ingeniería de técnicas reproductivas nuevas e innovativas para ser utilizadas a nivel de granja. Estas técnicas reproductivas deberían ser desarrolladas respetando los tres pilares de la sostenibilidad: el ambiente, la economía y la sociedad. Por ello deberían estar incluidas dentro de los sistemas de producción en los cuales se supone que serán aplicadas y donde deberían ser evaluadas en su sostenibilidad.

Proposal to minimize the difficulty of recording abortion data in sheep

Background: The most frequent viral diseases which can cause abortion in sheep are Blue tongue, Border disease virus, Cache Valley fever and Schmallenberg virus. The diagnosis of abortion, namely virus-induced represents a challenge to field clinicians, since clinical signs presented by the dam are discrete, non-specific and variable (Agerhom et al., 2015). On the other hand, while some foetuses reveal characteristic and visible malformations, others do not reveal any lesions. In face of it, definitive diagnosis requires an appropriate history collection, as well as sending fresh samples, namely abortion material, foetus, placenta and umbilical cord, to a specialty laboratory, to obtain a precise diagnosis. Objectives: The authors suggest a registration method of all mandatory data, in order to further assist the diagnosis of viral diseases at the laboratories, including the most frequent congenital malformations reported in sheep abortions. Methods: Abortion samples of suspected viral origin were collected and all data were registered, in worktables optimized for this purpose. Results: The authors document, using macroscopic figures lesions of malformations in abortions, emphasizing the frequency and the importance of documenting each case, proposing practical and effective worktables to assist the fieldwork. Conclusions: Field clinician’s awareness of the importance of early detection of viral diseases causing abortion outbreaks stimulates a proper data collection for each case of abortion, in order to contribute to a precise diagnosis and posterior consistent epidemiological studies, which may allow diminishing of economic losses.

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Due to economical and scientific limitations, sheep embryo reproductive technologies are less commercially applied than in other animal species. However, it is very clear that, in the near future, those techniques are expected to have a central role in animal production as a consequence of genetic and reproductive demands. One drawback is that results obtained after sheep embryo cryopreservation are unattractive for commercial purposes. It is expected that a successful cryopreservation of sheep embryos can push forward all other reproductive biotechnologies in this species, such as multiple ovulation and embryo transfer (MOET), artificial insemination, or in vitro production of embryos. This paper tries to discuss the current and future perspectives of cryopreservation of in vivo- and in vitro-produced sheep embryos concerning advantages and limitations for its practical use and possible solutions for improving methods to allow a higher survival rate of cryopreserved embryos.

Doppler ultrasound in the diagnosis of early pregnancy in sheep.

2016