(Table 1) Concentrations and carbon isotopic compositions of CH4 and CO2 at DSDP Site 76-533

The principal gaseous carbon-containing components identified in the first 400 m of sediment at Deep Sea Drilling Project Site 533, Leg 76, are methane (CH4) and carbon dioxide (CO2). Below a sub-bottom depth of about 25 m, sediment cores commonly contained pockets caused by the expansion of gas upon core recovery. The carbon isotopic composition (d13C per mil relative to PDB standard) of CH4 and CO2 in these gas pockets has been measured, resulting in the following observations: (1) d13C-CH4 values increase with depth from approximately -94 per mil in the uppermost sediment to about -66 per mil in the deepest sediment, reflecting a systematic but nonlinear depletion of 12C with depth. (2) d13C-CO2 values also increase with depth of sediment from about -25 per mil to about -4 per mil, snowing a depletion of 12C that closely parallels the trend of the isotopic composition of CH4. The magnitude and parallel distribution of d13C values for both CH4 and CO2 are consistent with the concept that the formation of the CH4 resulted from the microbiological reduction of CO2 from organic substances. These results imply that CH4 and CO2 incorporated in gas hydrates at this site are biogenic.

(Table 1) Compositional variation with depth in a nodule of sediment sample RVG-10D

A detailed study of a nodule from the Somali Basin dated by 230Thexcess was correlated with the paleoceanographic events recorded in Site 236 (Leg 24) Deep Sea Drilling Project (DSDP) cores. Tentative indications are that the phase of nodule accretion starting with the development of pillar structure at a depth of 20 mm in the nodule around 13 Ma coincides with increased Antarctic Bottom Water (AABW) flow and an elevated calciumcarbonate compensation depth (CCD). The Late Miocene lowering of the CCD is represented by the mottled zones between 8 and 18 mm in the nodule is characterised by an abundant silicate component (>20%) of aeolian origin. The Miocene/Pliocene boundary (5 Ma) occurs at a depth of about 8 mm and is represented by the development of pillar structure and a minimum of aeolian dust (10.3%). The increased biological productivity of the Somali surface water since the Middle Miocene is demonstrated by the increasing Corg content of the nodule (from 0.11 to 0.19%) towards its surface.

Sample information and classification of lower Miocene gravity-flow types of sediment core CRP-1 (Table 1)

Nineteen samples of the Cape Roberts-1 drillcore were taken from Miocene- age deposits, from 90.25 - 146.50 metres below seafloor (mbsf) for thin section and laser grain-size analysis. Using the grain-size distribution, detailed core logging, X-radiography and thin-section analysis of microstructures, coupled with a statistical grouping of the grain-size data, three main styles of gravity-flow sedimentation were revealed. Thin (centimetre-scale) muddy debris-flow deposits are the most common and are possibly tirggered by debris rain-out from sea-ice These deposits are characterised by very poorly sorted, faintly laminated muddy sandstones with coarse granules toward their base. Contacts are gradational to sharp. Variations on this style of mass-wasting deposit are rhythmically stacked sequences of pebbly-coarse sandstones representing successive thin debris-flow events. These suggest very high sedimentation rates on an unstable slope in a shallow-water proximal glacimarine environment. Sandy-silty turbidites appear more common in the lower sections of the core, below approximately 141.00 mbsf, although they occur occasionally with the debris flow deposits The turbidites are characterised by inversely to normally graded, well-laminated siltstones with occasional lonestones, and represent a more distal shallow-water glacimarine environment.

Operator's manual : radiosonde recorders, AN/TMQ-5 (NSN 6660-00-324-9426), AN/TMQ-5A (NSN 6660-00-393-2234), AN/TMQ-5B (NSN 6660-00-393-2234), AN/TMQ-5C (NSN 6660-00-682-4500).

Includes index.

Miscellaneous parks and public land measures : hearing before the Subcommittee on Public Lands, National Parks, and Forests of the Committee on Energy and Natural Resources, United States Senate, One Hundred First Congress, first session, on S. 319, S. 374, S. 393, S. 620, S. 1230, H.R. 2783, September 12, 1989.

Shipping list no.: 90-134-P (pt. 1).

(Table 1) Ion concentrations in the haemolymph of Apherusa glacialis after incubation at different medium salinities

Amphipods living at the underside of Arctic sea ice are exposed to varying salinities due to freezing and melting, and have to cope with the resulting osmotic stress. Extracellular osmotic and ionic regulation at different salinities, thermal hysteresis, and supercooling points (SCPs) were studied in the under-ice amphipod Apherusa glacialis. The species is euryhaline, capable to regulate hyperosmotically at salinities S(R) < 30 g/kg, and osmoconforms at salinities S(R) >= 30 g/kg. Hyperosmotic regulation is an adaptation to thrive in low-salinity meltwater below the ice. Conforming to the ambient salinity during freezing reduces the risk of internal ice formation. Thermal hysteresis was not observed in the haemolymph of A. glacialis. The SCP of the species was -7.8 ± 1.9°C. Several ions were specifically downregulated ([Mg2+], [SO4]2-), or upregulated ([K+], [Ca2+]) in comparison to the medium. Strong downregulation of [Mg2+], is probably necessary to avoid an anaesthetic effect at low temperatures.

Downwelling spectral irradiance as measured in different depths, remote sensing reflectance and phytoplankton pigment concentration during POLARSTERN cruises ANT-XXIV/1, ANT-XXIV/4, ANT-XXVI/4 and Maria S. Merian cruise MSM18/3

The composition and abundance of algal pigments provide information on phytoplankton community characteristics such as photoacclimation, overall biomass and taxonomic composition. In particular, pigments play a major role in photoprotection and in the light-driven part of photosynthesis. Most phytoplankton pigments can be measured by high-performance liquid chromatography (HPLC) techniques applied to filtered water samples. This method, as well as other laboratory analyses, is time consuming and therefore limits the number of samples that can be processed in a given time. In order to receive information on phytoplankton pigment composition with a higher temporal and spatial resolution, we have developed a method to assess pigment concentrations from continuous optical measurements. The method applies an empirical orthogonal function (EOF) analysis to remote-sensing reflectance data derived from ship-based hyperspectral underwater radiometry and from multispectral satellite data (using the Medium Resolution Imaging Spectrometer - MERIS - Polymer product developed by Steinmetz et al., 2011, doi:10.1364/OE.19.009783) measured in the Atlantic Ocean. Subsequently we developed multiple linear regression models with measured (collocated) pigment concentrations as the response variable and EOF loadings as predictor variables. The model results show that surface concentrations of a suite of pigments and pigment groups can be well predicted from the ship-based reflectance measurements, even when only a multispectral resolution is chosen (i.e., eight bands, similar to those used by MERIS). Based on the MERIS reflectance data, concentrations of total and monovinyl chlorophyll a and the groups of photoprotective and photosynthetic carotenoids can be predicted with high quality. As a demonstration of the utility of the approach, the fitted model based on satellite reflectance data as input was applied to 1 month of MERIS Polymer data to predict the concentration of those pigment groups for the whole eastern tropical Atlantic area. Bootstrapping explorations of cross-validation error indicate that the method can produce reliable predictions with relatively small data sets (e.g., < 50 collocated values of reflectance and pigment concentration). The method allows for the derivation of time series from continuous reflectance data of various pigment groups at various regions, which can be used to study variability and change of phytoplankton composition and photophysiology.

Galanin and galanin fragment 1-15 modulate antidepressant responses by targeting 5-HT1AR-GALR heteroreceptor complexes of the ascending midbrain serotonin pathways.

Mood disorders, including depression and anxiety, are among the most prevalent mental illnesses with high socioeconomic impact. Although the underlying mechanisms have not yet been clearly defined in the last decade the importance of the role of neuropeptides, including Galanin (GAL), and/or their receptors in the treatment of stress-related mood disorders is becoming increasingly apparent. GAL is involved in mood regulation, including depression-related and anxiety-like behaviors. Activation of GALR1 and GALR3 receptors results in a depression like behavior while stimulation of GALR2 receptor leads to anti-depressant-like effects. Moreover, GAL modulates 5-HT1A receptors (5-HT1AR), a key receptor in depression at autoreceptor and postsynaptic level in the brain. This interaction can in part be due to the existence of GALR1-5-HT1AR heteroreceptor complexes in discrete brain regions [1]. Not only GAL but also the N-terminal fragments like GAL(1-15) are active in the Central Nervous System [2, 3]. Recently, we described that GAL(1-15) induces strong depression-related and anxiogenic-like effects in rats, and these effects were significantly stronger than the ones induced by GAL [4]. The GALR1-GALR2 heteroreceptor complexes in the dorsal hippocampus and especially in the dorsal raphe (DR), areas rich in GAL(1-15) binding sites [5] were involved in these effects [4, 6] and demonstrated also in cellular models. In the present study, we have analyzed the ability of GAL(1-15) to modulate 5-HT1AR located at postjunctional sites and at the soma-dendritic level in rats. We have analyzed the effect of GAL(1-15) on the 5-HT1AR-mediated response in a behavioral test of depression and the involvement of the GALR2 in these effects. GAL(1-15) enhanced the antidepressant effects induced by the 5-HT1AR agonist 8-OH-DPAT in the forced swimming test [7]. These effects were stronger than the ones induced by GAL. The mechanism of this action involved interactions at the receptor level in the plasma membrane with changes also at the transcriptional level. Thus, GAL(1-15) affected the binding characteristics as well as the mRNA level of 5-HT1AR in the dorsal hippocampus and DR. GALR2 was involved in these effects, since the specific GALR2 antagonist M871 blocked GAL(1-15) mediated actions at the behavioral and receptor level [7]. Furthermore, the results on the proximity ligation assay (PLA) in this work suggest the existence of GALR1-GALR2-5-HT1AR heteroreceptor complexes since positive PLA were obtained for both GALR1-5-HT1AR and GALR2-5-HT1AR complexes in the DR and hippocampus. Moreover the studies on RN33B cells, where GALR1, GALR2 and 5-HT1AR exist [4], also showed PLA-positive clusters indicating the existence of GALR1-5-HT1AR and GALR2-5-HT1AR complexes in these cells [7]. In conclusion, our results indicate that GAL(1–15) enhances the antidepressant effects induced by the 5-HT1AR agonist 8-OH-DPAT probably acting on GALR1-GALR2-5-HT1AR heteroreceptor located at postjunctional sites and at the soma-dendritic level. The development of new drugs specifically targeting these heteroreceptor complexes may offer a novel strategy for treatment of depression. This work has been supported by Junta de Andalucia CVI646 1. Borroto-Escuela, D.O., et al., Galanin receptor-1 modulates 5-hydroxtryptamine-1A signaling via heterodimerization. Biochem Biophys Res Commun, 2010. 393(4): p. 767-72. 2. Hedlund, P.B. and K. Fuxe, Galanin and 5-HT1A receptor interactions as an integrative mechanism in 5-HT neurotransmission in the brain. Ann N Y Acad Sci, 1996. 780: p. 193-212. 3. Diaz-Cabiale, Z., et al., Neurochemical modulation of central cardiovascular control: the integrative role of galanin. EXS, 2010. 102: p. 113-31. 4. Millon, C., et al., A role for galanin N-terminal fragment (1-15) in anxiety- and depression-related behaviors in rats. Int J Neuropsychopharmacol, 2015. 18(3). 5. Hedlund, P.B., N. Yanaihara, and K. Fuxe, Evidence for specific N-terminal galanin fragment binding sites in the rat brain. Eur J Pharmacol, 1992. 224(2-3): p. 203-5. 6. Borroto-Escuela, D.O., et al., Preferential activation by galanin 1-15 fragment of the GalR1 protomer of a GalR1-GalR2 heteroreceptor complex. Biochem Biophys Res Commun, 2014. 452(3): p. 347-53. 7. Millon, C., et al., Galanin (1-15) enhances the antidepressant effects of the 5-HT1A receptor agonist 8-OH-DPAT: involvement of the raphe-hippocampal 5-HT neuron system. Brain Struct Funct, 2016.

Fenton-like degradation of Bisphenol A catalyzed by mesoporous Cu/TUD-1

A family of copper oxide catalysts with loadings spanning 1–5 wt% were dispersed on a three dimensional, mesoporous TUD-1 silica through a hydrothermal, surfactant-free route employing tetraethylene glycol as a structure-directing agent. Their bulk and surface properties were characterized by N2 physisorption, XRD, DRUVS, EPR, TEM and Raman spectroscopy, confirming the expected mesoporous wormhole/foam support morphology and presence of well-dispersed CuO nanoparticles (∼5–20 nm). The catalytic performance of Cu/TUD-1 was evaluated as heterogeneous Fenton-like catalysts for Bisphenol A (BPA) oxidative degradation in the presence of H2O2 as a function of [H2O2], and CuO loading. Up to 90.4% of 100 ppm BPA removal was achieved over 2.5 wt% Cu/TUD-1 within 180 min, with negligible Cu leaching into the treated water.