Evaluation of four molecular typing Methodologies as tools for determining taxonomy relations and for identifying. species among Yersinia isolates

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Amniotic Fluid Interleukin-1 Beta and Interleukin-6, but not Interleukin-8 Correlate with Microbial Invasion of the Amniotic Cavity in Preterm Labor

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Uncommon mandibular osteomyelitis in a cat caused by Nocardia africana

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evolutionary dynamics of heterochromatin in the genome of Dichotomius beetles based on chromosomal analysis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

GENETIC INTERRELATIONSHIPS OF PROTEOLYTIC CLOSTRIDIUM-BOTULINUM TYPE-A, TYPE-B, AND TYPE-F AND OTHER MEMBERS OF THE CLOSTRIDIUM-BOTULINUM COMPLEX AS REVEALED BY SMALL-SUBUNIT RIBOSOMAL-RNA GENE-SEQUENCES

The phylogenetic interrelationships of members of the Clostridium botulinum complex of species was investigated by direct sequencing of their 16S rRNA genes. Comparative analysis of the 16S rRNA sequences demonstrated the presence of four phylogenetically distinct lineages corresponding to: i) proteolytic C. botulinum types Al B, and F, and C. sporogenes, ii) saccharolytic types B, E and F, iii) types C and D and C. novyi type A, and iv) type G and C. subterminale. The phylogenetic groupings obtained from the 16S rRNA were in complete agreement with the four divisions recognised within the 'species complex' on the basis of phenotypic criteria.

Phylogenetic analysis of flatfish (order pleuronectiformes) based on mitochondrial 16s rDNA sequences

The phylogenetic relationships of the order Pleuronectiformes are controversial and at some crucial points remain unresolved. To date most phylogenetic studies on this order have been based on morpho-anatomical criteria, whereas only a few sequence comparisons based studies have been reported. In the present study, the phylogenetic relationships of 30 flatfish species pertaining to seven different families were examined by sequence analysis of the first half of the 16S mitochondrial DNA gene. The results obtained did not support percoids as the sister group of pleuronectiforms. The monophyletic origin of most families analyzed, Soleidae, Scophthalmidae, Achiridae, Pleuronectidae and Bothidae, was strongly supported, except for Paralichthyidae which was clearly subdivided into two groups, one of them associated with high confidence to Pleuronectidae. The analysis of the 16S rRNA gene also suggested the monophyly of Pleuronectiforms as the most probable hypothesis and consistently supported some major interfamily groupings.

Comunidades bacterianas em solos de mata nativa e cultivados com cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Isolamento e identificação de bactérias solubilizadoras de fósforo e produtoras de auxinas em solo com cana-de-açúcar

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Isolamento, identificação e inoculação de bactérias produtoras de auxinas associadas às raízes de orquídeas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Isolamento de bactérias produtoras de enzimas de interesse em processos biotecnológicos

Pós-graduação em Microbiologia Agropecuária - FCAV

Diversidade bacteriana em piscicultura neotropical

Pós-graduação em Microbiologia Agropecuária - FCAV

Indução da expressão in vivo e caracterização cinética da fosfatase ácida de Enterobacter sp. isolada de raízes de orquidáceas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Prospecção de rizobactérias promotoras de crescimento em quatro espécies arbóreas nativas do Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Prospecção gênica e diversidade bacteriana de um consórcio degradador de óleo diesel

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Simbioses de rizóbios com leguminosas arbóreas na pré-amazônia maranhense

The tree leguminous gliricídia (Gliricidia sepium), acácia (Acacia mangium), leucena (Leucaena leucocephala) and sombreiro (Clitoria fairchildiana) are indicated for agroforestry systems, reclamation of degraded lands, reforestation and other purposes in the wet tropic. Despite the importance of legumes the preamazon region it is lacking in information about the symbiotic capacity and diversity of indigenous rhizobia of these legumes. The aim of this work was to evaluate the phenotypic and genetic diversity of rhizobia species nodulating gliricidia sombreiro, leucena and acacia in the Maranhão pre-Amazon region and authenticate isolates of these species in siratro (Macroptilium atropurpureum). For this they were installed two experiments. Sampling was carried out on a alley cropping system, was sampled 20 plants of each species by collecting 10 nodules per plant. It was made isolation, cultural characterization, partial 16S rRNA gene sequencing and analysis of the symbiotic ability of bacterial strains with siratro. The authentication experiment was done in two steps for each legume (gliricidia, acácia, sombreiro and leucena), in the greenhouse and in a completely randomized design with three replications with sterile Hoagland nutrient solution as substrate. Gliricídia, Sombreiro, leucena and acacia are colonized by distinct groups of rhizobia. Gliricidia nodulate preferably with Rhizobium, sombreiro and acacia nodulate preferably with Bradyrhizobium and leucena has Mesorhizobium main symbiote. Endophytic strains of ten genera were found colonizing the gliricidia, sombrero, leucena and acacia nodules and a strain of Arthrobacter sp. had a positive nodulation with siratro. This is the first report on isolation of Methylobacterium sp. in gliricidia nodules and endophytic ability of Terriglobus sp. strains. Indigenous strains of pre-Amazon region of Bradyrhizobium, Mesorhizobium and Rhizobium genus nodulate with siratro, but are ineficiente or had low efficiency to promote their growth.