STAT3α interacts with nuclear GSK3beta and cytoplasmic RISK pathway and stabilizes rhythm in the anoxic-reoxygenated embryonic heart.

Activation of the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 (JAK2/STAT3) pathway is known to play a key role in cardiogenesis and to afford cardioprotection against ischemia-reperfusion in adult. However, involvement of JAK2/STAT3 pathway and its interaction with other signaling pathways in developing heart transiently submitted to anoxia remains to be explored. Hearts isolated from 4-day-old chick embryos were submitted to anoxia (30 min) and reoxygenation (80 min) with or without the antioxidant MPG, the JAK2/STAT3 inhibitor AG490 or the PhosphoInositide-3-Kinase (PI3K)/Akt inhibitor LY-294002. Time course of phosphorylation of STAT3α(tyrosine705) and Reperfusion Injury Salvage Kinase (RISK) proteins [PI3K, Akt, Glycogen Synthase Kinase 3beta (GSK3beta), Extracellular signal-Regulated Kinase 2 (ERK2)] was determined in homogenate and in enriched nuclear and cytoplasmic fractions of the ventricle. STAT3 DNA-binding was determined. The chrono-, dromo- and inotropic disturbances were also investigated by electrocardiogram and mechanical recordings. Phosphorylation of STAT3α(tyr705) was increased by reoxygenation, reduced (~50%) by MPG or AG490 but not affected by LY-294002. STAT3 and GSK3beta were detected both in nuclear and cytoplasmic fractions while PI3K, Akt and ERK2 were restricted to cytoplasm. Reoxygenation led to nuclear accumulation of STAT3 but unexpectedly without DNA-binding. AG490 decreased the reoxygenation-induced phosphorylation of Akt and ERK2 and phosphorylation/inhibition of GSK3beta in the nucleus, exclusively. Inhibition of JAK2/STAT3 delayed recovery of atrial rate, worsened variability of cardiac cycle length and prolonged arrhythmias as compared to control hearts. Thus, besides its nuclear translocation without transcriptional activity, oxyradicals-activated STAT3α can rapidly interact with RISK proteins present in nucleus and cytoplasm, without dual interaction, and reduce the anoxia-reoxygenation-induced arrhythmias in the embryonic heart.

El crowdfunding: Claves para la financiación colectiva de un álbum musical en la plataforma de internet ‘Verkami’

El estudio tiene por objeto, la comprensión de la forma en que los artistas llevan a cabo sus proyectos, el conocimiento de las razones del uso del crowdfunding y la importancia de dicha práctica en la viabilidad del proyecto. La obtención de una visión amplia del desarrollo del fenómeno en el ámbito musical de España, atendiendo a las tendencias existentes y a qué tipo de artistas recurren a dicha práctica y el entendimiento del modelo de funcionamiento de ‘Verkami’: sus características, requisitos y los motivos de su elección frente a otras plataformas.

L'ensenyament musical: presentació (monografia)

Quan es parla d'estudiar música, sovint ens ve al cap la paraula conservatori; hi estem tan acostumats que ni ens adonem del caracter conservador que anomenem al principal centre on s'estudia la nostra materia. En aquest context, vèncer aquest immobilisme i infondre un esperit més actual en l'ensenyament de la música, no ha estat gens fàcil, ja que les institucions sempre han procurat evitar ficar-se en terrenys incerts. Sia per desconeixement, sia per falta d'infraestructures adequades, de suficients medis o de personal qualificat, hem hagut d'esperar a l'aplicació de la LOGSE per assistir a una veritable normalització de la música en l'ensenyament obligatori, a la vegada que s'aconseguia actualitzar els ensenyaments artisticomusicals impartits en els «conservatoris» esmentats.

Annuaire des artistes et de l'enseignement dramatique et musical

1906 (A20).

Annuaire des artistes et de l'enseignement dramatique et musical

1893 (A7).

Annuaire des artistes et de l'enseignement dramatique et musical

1894 (A8).

Annuaire des artistes et de l'enseignement dramatique et musical

1897 (A11).

Annuaire des artistes et de l'enseignement dramatique et musical

1910 (A24).

Annuaire des artistes et de l'enseignement dramatique et musical

1909 (A23).

Annuaire des artistes et de l'enseignement dramatique et musical

1903 (A17).

Annuaire des artistes et de l'enseignement dramatique et musical

1907 (A21).

Annuaire des artistes et de l'enseignement dramatique et musical

1895 (A9).