Tissue engineering: technological advances to improve its applications in reconstructive surgery

Background. Tremendous advances in biomaterials science and nanotechnologies, together with thorough research on stem cells, have recently promoted an intriguing development of regenerative medicine/tissue engineering. The nanotechnology represents a wide interdisciplinary field that implies the manipulation of different materials at nanometer level to achieve the creation of constructs that mimic the nanoscale-based architecture of native tissues. Aim. The purpose of this article is to highlight the significant new knowledges regarding this matter. Emerging acquisitions. To widen the range of scaffold materials resort has been carried out to either recombinant DNA technology-generated materials, such as a collagen-like protein, or the incorporation of bioactive molecules, such as RDG (arginine-glycine-aspartic acid), into synthetic products. Both the bottom-up and the top-down fabrication approaches may be properly used to respectively obtain sopramolecular architectures or, instead, micro-/nanostructures to incorporate them within a preexisting complex scaffold construct. Computer-aided design/manufacturing (CAD/CAM) scaffold technique allows to achieve patient-tailored organs. Stem cells, because of their peculiar properties - ability to proliferate, self-renew and specific cell-lineage differentiate under appropriate conditions - represent an attractive source for intriguing tissue engineering/regenerative medicine applications. Future research activities. New developments in the realization of different organs tissue engineering will depend on further progress of both the science of nanoscale-based materials and the knowledge of stem cell biology. Moreover the in vivo tissue engineering appears to be the logical step of the current research.

Conocimientos, actitudes y prácticas en la salud sexual y la salud reproductiva: propuesta de una escala psicométrica

En este artículo se presenta el resultado del análisis de validez y confiabilidad de las escalas propuestas para medir conocimientos, actitudes y prácticas en salud sexual y salud reproductiva de la población estudiantil de la Universidad Nacional. La escala inicial estuvo conformada por 288 ítemes divididos en cuatro dimensiones (salud sexual, salud reproductiva, VIH/ SIDA e infecciones de transmisión sexual y uso del condón) y cada dimensión en tres categorías (conocimientos, actitudes y prácticas). El tratamiento de la escala inició con un análisis de validez aparente a partir de la evaluación de 12 personas expertas en la temática, luego se procedió con un análisis de factores, uno de correlación y se terminó con un análisis de confiabilidad que arrojó un Alfa de Cronbach de 0.9290, para concluir que la escala tiene una excelente consistencia interna. Finalmente, se construyó una nueva escala con 128 ítemes agrupados en las tres categorías y las cuatro dimensiones propuestas. Abstract In this document, the results of the validity and reliability analysis of the scales propose to measure knowledge, attitudes and practices in sexual health and reproductive health of the students of the Universidad Nacional, are presented. The scale was primarily composed of 288 items divided in four dimensions (sexual health, reproductive health, HIV/AIDS and sexual transmitted diseases, and condom use), and each dimension in three categories (knowledge, attitudes and practices). The treatment of the scale began with a face validity analysis from the evaluation of 12 experts and then with a factor analysis, correlation analysis and finished with a reliability analysis that gave a result a Cronbach coefficient of 0.9290, leading to the conclusion that the scale has an excellent internal consistency. Finally, a new scale was constructed with 128 items, gathered in the three categories and four dimensions proposed.

Acompanhamento da construção de uma clínica de hemodiálise

Relatório de Estágio para obtenção do grau de Mestre em Engenharia Civil

Canções de fogo: a apreensão de saberes através da performance da escuta no contexto da União do Vegetal

O ritual onde se utiliza a cocção conhecida por ayahuasca vem sendo ressignificado em contextos urbanos há mais de oito décadas, sobretudo após sua expansão iniciada pelos movimentos organizados. Essa experiência derivante dos povos da floresta amazônica, vem ultrapassando tanto fronteiras geográficas como culturais, sendo constantemente reinventada por diferentes agentes. Contemporaneamente, o fenômeno abarca indivíduos provenientes de várias partes do mundo incorporando legados doutrinários de mestres originários da América Latina. A linha doutrinária conhecida por União do Vegetal - UDV, na qual este trabalho se centra, vem ganhando espaço entre os circuitos europeus neo esotéricos. Atualmente o ritual é vivenciado por mais de 18 mil pessoas espalhadas pelo globo e tem como característica, após a ingestão da cocção, a utilização da música como uma das ferramentas mediadoras para a apreensão dos princípios doutrinários. Usualmente os mestres da UDV têm como característica a utilização de fonogramas de variadas categorias musicais que adquirem uma dimensão simbólica e funcional no momento do ritual. A comunidade estabelecida em Portugal - foco desta tese – e as comunidades digitais Musincante e Músicas do Alto, permitiram-me perceber, através do trabalho de campo e de observação participante, elementos constitutivos que abrangem toda a comunidade da UDV. O enfoque parte da perspectiva de observar essa doutrina como um lugar de conhecimento sistematizado e formal. No sentido de compreender os processos de construção desse saber, foi verificado o protagonismo do ato da escuta como elemento central para a apreensão da doutrina. Neste sentido, esta tese adota o conceito de performance da escuta como meio de explorar as formas como o indivíduo e o coletivo absorvem um saber doutrinário a partir da música e de como esta assume o papel de mediadora dos saberes transmitidos no contexto da UDV e de manutenção deste conhecimento num contexto extra ritual.

O contributo da utilização do EdiLim, como recurso educativo na prática de ensino supervisionada, através da conceção de atividades no 1º ciclo do ensino básico

Relatório de Estágio apresentado à Escola Superior de Educação do Instituto Politécnico de Castelo Branco para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Educação Pré-Escolar e Ensino do 1º Ciclo do Ensino Básico.

Prática de ensino supervisionada em educação pré-escolar e ensino do 1º ciclo do ensino básico: a articulação integrada do saber

O presente relatório desenvolve-se no âmbito da unidade curricular de Prática de Ensino Supervisionada para obter o grau mestre em Educação Pré-escolar e Ensino do 1º Ciclo do Ensino Básico apresentando o trabalho de investigação-ação desenvolvido em contexto de Jardim de Infância e 1º Ciclo do Ensino Básico focalizado na articulação integrada do saber. Elegeram-se como objetivos deste trabalho compreender a natureza das aprendizagens nas primeiras idades, promovendo a construção articulada do saber evitando a sua disciplinarização, utilizando diversas linguagens e implementar a metodologia de projeto no trabalho curricular. O enquadramento teórico procura aprofundar conceitos de interdisciplinaridade e construção articulada do saber nos processos de aprendizagem e o trabalho de projeto enquanto matriz de construção de conhecimento. Sustentado na metodologia de investigaçãoação utilizaram-se diversos instrumentos de recolha e análise de dados como a observação, registo de notas de campo e entrevistas às crianças permitindo responder aos objetivos delineados. Este trabalho permitiu compreender a necessidade da construção articulada das aprendizagens para promover aprendizagens significativas; Supervised teaching Practice in Pre-school and Primary Education: Integrated Knowledge Construction Abstract: This is the report of the action-research project developed in the context of a Pre-school and a Primary School focused on the integration of knowledge, part of the curricular unit Supervised Teaching Practice of the Master in Pre-school Education and Teaching in Basic Education (1st cycle). The objectives of this work are: to understand the nature of learning in the early ages through not only promoting the construction of an articulated knowledge while avoiding its disciplinarization, but also using several languages and implement the project methodology in curricular work. The theoretical framework seeks to explore the concepts of interdisciplinarity and the integration of knowledge’s construction in the processes of learning as the project’s work as a basic framework of knowledge construction. Adopting an action-research methodology, several instruments were used for data collection and analysis, such as observation, field notes and interviews to children, allowing to reach the stipulated objectives. This study provided the understanding of the need for the learning’s articulated creation to promote relevant learning.

Determination of thresholds in marine molluscs as an alternative to the Environmental Quality Standards in marine water defined in the Water Framework Directive

The Water Framework Directive (WFD) establishes Environmental Quality Standards (EQS) in marine water for 34 priority substances. Among these substances, 25 are hydrophobic and bioaccumulable (2 metals and 23 organic compounds). For these 25 substances, monitoring in water matrix is not appropriate and an alternative matrix should be developed. Bivalve mollusks, particularly mussels (Mytilus edulis, Mytilus galloprovincialis), are used by Ifremer as a quantitative biological indicator since 1979 in France, to assess the marine water quality. This study has been carried out in order to determine thresholds in mussels at least as protective as EQS in marine water laid down by the WFD. Three steps are defined: - Provide an overview of knowledges about the relations between the concentrations of contaminants in the marine water and mussels through bioaccumulation factor (BAF) and bioconcentration factor (BCF). This allows to examine how a BCF or a BAF can be determined: BCF can be determined experimentally (according to US EPA or ASTM standards), or by Quantitative Activity-Structure Relationship models (QSAR): four equations can be used for mussels. BAF can be determined by field experiment; but none standards exists. It could be determined by using QSAR but this method is considered as invalid for mussels, or by using existing model: Dynamic Budget Model, but this is complex to use. - Collect concentrations data in marine water (Cwater) in bibliography for those 25 substances; and compare them with concentration in mussels (Cmussels) obtained through French monitoring network of chemicals contaminants (ROCCH) and biological integrator network RINBIO. According to available data, this leads to determine the BAF or the BCF (Cmussels /Cwater) with field data. - Compare BAF and BCF values (when available) obtained with various methods for these substances: BCF (stemming from the bibliography, using experimental process), BCF calculated by QSAR and BAF determined using field data. This study points out that experimental BCF data are available for 3 substances (Chlorpyrifos, HCH, Pentachlorobenzene). BCF by QSAR can be calculated for 20 substances. The use of field data allows to evaluate 4 BAF for organic compounds and 2 BAF for metals. Using these BAF or BCF value, thresholds in shellfish can be determined as an alternative to EQS in marine water.

Forensic Injustice: Human Rights, Archival Science and Racialized Feminicide in Guatemala

The call to access and preserve the state records that document crimes committed by the state during Guatemala’s civil war has become an archival imperative entangled with neoliberal human rights discourses of “truth, justice, and memory.” 200,000 people were killed and disappeared in Guatemala’s civil war including acts of genocide in which 85% of massacres involved sexual violence committed against Mayan women. This dissertation argues that in an attempt to tell the official story of the civil war, American Human Rights organizations and academic institutions have constructed a normative identity whose humanity is attached to a scientific and evidentiary value as well as an archival status representing the materiality and institutionality of the record. Consequently, Human Rights discourses grounded in Western knowledges, in particular archival science and law, which prioritize the appearance of truth erase the material and epistemological experience of indigenous women during wartimes. As a result, the subjectivity that has surfaced on the record as most legible has mostly pertained to non-indigenous, middle class, urban, leftist men who were victims of enforced disappearance not genocide. This dissertation investigates this conflicting narrative that remembers a non-indigenous revolutionary masculine hero and grants him justice in human rights courtrooms simply because of a document attesting to his death. A main research question addressed in this project is why the promise of "truth and justice" under the name of human rights becomes a contentious site for gendered indigenous bodies? I conduct a discursive and rhetorical analysis of documentary film, declassified Guatemalan police and military records such as Operation Sofia, a military log known for “documenting the genocide” during rural counterinsurgencies executed by the military. I interrogate the ways in which racialized feminicides or the hyper-sexualized racial violence that has historically dehumanized indigenous women falls outside of discourses of vision constructed by Western positivist knowledges to reinscribe the ideal human right subject. I argue for alternative epistemological frames that recognize genocide as sexualized and gendered structures that have simultaneously produced racialized feminicides in order to disrupt the colonial structures of capitalism, patriarchy and heterosexuality. Ironically, these structures of power remain untouched by the dominant human rights discourse and its academic, NGO, and state collaborators that seek "truth and justice" in post-conflict Guatemala.

A Feminist Affective Turn for Public Relations: Mothers, Passionate Publics, and the Childhood Vaccine Debate

This project proposes a feminist intervention in how affect and publics are theorized in public relations research. Drawing from extant literature, I argue that public relations theories of affect and publics have been apolitical and lack depth and context (Leitch & Motion, 2010a). Using the context of the online childhood vaccine debate, I illustrate several theories and concepts of the new feminist affective turn, as well as postmodern theories of affect, relevant to public relations research: (a) Public Feelings, “ugly” feelings, agency, and community (Cvetkovich, 2012; Ngai, 2007); (b) passionate politics (Mouffe, 2014); (c) postmodern assemblages, biopower, and body politics (Deleuze & Guattari, 1988; Foucault, 1984); (d) affective facts and logics of future threats (Massumi, 2010); and (e) affective ethics (Bertleson & Murphie, 2010). Scholarship in the areas of public relations, risk, feminist and postmodern affect theory, and the vaccine debate provided theoretical grounding for this project. My research questions asked: How is feminist affect theory embodied by mothers in the vaccine debate? How do mothers understand risks as affective facts in the vaccine debate (if at all)? What affective logics are used by mothers in the vaccine debate (if any)? And, What are sources of knowledge for mothers in the vaccine debate? Multi-sited online ethnographic methods were used to explore how feminist affect theory contributes to public relations research, including 29 one-on-one in-depth interviews with mothers of young children and participant observation of 15 online discussions about vaccines on parenting websites BabyCenter.com, TheBump.com, and WhatToExpect.com. I used snowball sampling to recruit interview participants and grounded theory (Glaser & Strauss, 1967) to analyze interview and online data. Results show that feminist affect theory contributes to theoretical and practical knowledge in public relations by politicizing and contextualizing understandings of publics and elucidating how affective facts and logics inform publics’ knowledge and choices, specifically in the context of risk. I also found evidence of suppression of dissent (Martin, 2015) and academic bias in vaccine debate research, which resulted in cultures of silence. Further areas of study included how specific contexts such as motherhood and issues of privilege and access affect publics’ experiences, knowledges, and choices.

El aprendizaje basado en problemas (ABP) para la enseñanza de las ciencias naturales en quinto año de educación general básica

La presente investigación pretende valorar la importancia de enseñar Ciencias Naturales a través de la estrategia del Aprendizaje Basado en Problemas (ABP), con la finalidad de mejorar las prácticas pedagógicas de los docentes, y a partir de la implementación del ABP desarrollar en los estudiantes aprendizajes significativos. Para esta investigación se utilizó una recopilación bibliográfica y se tomó como principal punto de partida el ABP y la Actualización y Fortalecimiento Curricular del 2010 en Ciencias Naturales, con la finalidad de responder a las siguientes preguntas: ¿Es posible planificar las clases de Ciencias Naturales a partir del ABP? ¿Cuáles son los pasos a seguir para implementar el ABP en Ciencias Naturales? Los resultados muestran que es posible implementar el ABP en las clases de Ciencias Naturales porque permite construir el nuevo conocimiento sobre la base de los conocimientos previos. En Ciencias Naturales es imprescindible generar en los estudiantes actitudes de protección y cuidado hacia el medio, aspectos que se consigue mediante la solución de problemas. Finalmente, se hizo una integración de varios autores sobre los pasos que se deben seguir para la implementación del ABP en Ciencias Naturales, el mismo que se organizó en nueve pasos.

A psicologia e o psicólogo do esporte : uma formação necessária

Dissertação (mestrado)—Universidade de Brasília, Instituto de Psicologia, Programa de Pós-graduação em Processos de Desenvolvimento Humano e Saúde, 2016.

A PHENOMENOLOGY OF MIMETIC LEARNING AND MULTIMODAL COGNITION: INTEGRATING EXPERIENTIAL KNOWLEDGE INTO PROGRAMS IN RHETORIC, COMPOSITION, AND TECHNICAL COMMUNICATION

My dissertation emphasizes a cognitive account of multimodality that explicitly integrates experiential knowledge work into the rhetorical pedagogy that informs so many composition and technical communication programs. In these disciplines, multimodality is widely conceived in terms of what Gunther Kress calls “socialsemiotic” modes of communication shaped primarily by culture. In the cognitive and neurolinguistic theories of Vittorio Gallese and George Lakoff, however, multimodality is described as a key characteristic of our bodies’ sensory-motor systems which link perception to action and action to meaning, grounding all communicative acts in knowledge shaped through body-engaged experience. I argue that this “situated” account of cognition – which closely approximates Maurice Merleau-Ponty’s phenomenology of perception, a major framework for my study – has pedagogical precedence in the mimetic pedagogy that informed ancient Sophistic rhetorical training, and I reveal that training’s multimodal dimensions through a phenomenological exegesis of the concept mimesis. Plato’s denigration of the mimetic tradition and his elevation of conceptual contemplation through reason, out of which developed the classic Cartesian separation of mind from body, resulted in a general degradation of experiential knowledge in Western education. But with the recent introduction into college classrooms of digital technologies and multimedia communication tools, renewed emphasis is being placed on the “hands-on” nature of inventive and productive praxis, necessitating a revision of methods of instruction and assessment that have traditionally privileged the acquisition of conceptual over experiential knowledge. The model of multimodality I construct from Merleau-Ponty’s phenomenology, ancient Sophistic rhetorical pedagogy, and current neuroscientific accounts of situated cognition insists on recognizing the significant role knowledges we acquire experientially play in our reading and writing, speaking and listening, discerning and designing practices.

Diferenciação pedagógica nas primeiras idades : visão dos profissionais de educação

Relatório final apresentado para a obtenção do grau de Mestre em Educação Pré-Escolar e em Ensino do 1.º Ciclo do Ensino Básico

Promoção de competências autorregulatórias da aprendizagem das crianças

Relatório final apresentado para a obtenção do grau de mestre em Educação Pré-Escolar e em Ensino do 1.º Ciclo do Ensino Básico

Diferenciação pedagógica nas primeiras idades : visão dos profissionais de educação

Relatório final apresentado para a obtenção do grau de Mestre em Educação Pré-Escolar e em Ensino do 1.º Ciclo do Ensino Básico