Efeitos do ultrassom de alta intensidade sob pressão na redução de Listeria monocytogenes em extrato de carne bovina

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Avaliação dos controles microbiológicos e do programa de redução de patógenos no abate de bovinos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Produção de lipase pelo fungo Myceliophthora sp. F. 2.1.4, caracterização e imobilização da solução enzimática bruta

Pós-graduação em Microbiologia - IBILCE

Aproveitamento de subprodutos agroindustriais para a produção de amilases fúngicas: estudo de parâmetros fermentativos e caracterização das enzimas

Pós-graduação em Microbiologia Agropecuária - FCAV

Desenvolvimento de novas tecnologias para produção de xarope de glicose a partir do amido

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Utilização de enzimas produzidas por Trichoderma reesei E Aspergillus niger na extração de óleos essenciais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização da lama vermelha e sua aplicabilidade na adsorção do corante têxtil Reativo Azul 19

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Correlação da coalescência de microcavidades com as propriedades mecânicas e elétricas de fios para Tx e Dx de energia elétrica da liga base Al-0,5%Cu-[0,24-0,28]%Fe modificada com teores de silício

Neste trabalho, é feita uma tentativa para estabelecer a influência dos teores [0,5; 0,7 e 0,9]%Si, como agentes modificadores da liga Al-0,05%Cu-[0,24-0,28]%Fe, avaliada através de aspectos que levaram em conta parâmetros operacionais de vazamentos unidirecionais horizontais tais como velocidades (Vs) e taxas de solidificação (Tx). Após operações de corte e usinagem foram obtidos perfis cilíndricos, com diâmetros de 9,5mm e comprimento de 120mm, a partir dos quais, após operações de trabalho à frio, chegou-se a fios com diâmetros de 2,7; 3,0; 3,8 e 4,0mm. Estes perfis foram submetidos à caracterização elétrica, com base na condutividade elétrica, à caracterização mecânica, com base em ensaios tensão/deformação enfatizando o alongamento, e a caracterização estrutural em seções longitudinais, com ênfase na distribuição das partículas de segunda fase e no aspecto da fratura, na qual a metodologia de avaliação das dimensões das microcavidades se utiliza da razão do cumprimento (L) pela largura (W).

Production and Characterization of a Milk-clotting Protease Produced in Submerged Fermentation by the Thermophilic Fungus Thermomucor indicae-seudaticae N31

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Construção de um equipamento de ensaio de desgaste micro abrasivo por esfera rotativa fixa para análise do desgaste em revestimento duro aplicado por soldagem

Pós-graduação em Engenharia Mecânica - FEIS

Purificação e estudos bioquímicos de exo-poligalacturonase (pectinase) produzida pelo fungo termofílico Rhizomucor pusillus, em cultivo submerso e aplicação na extração de sucos de frutas e hidrólise enzimática do bagaço de cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fixation of mandibular fractures with 2.0-mm miniplates: review of 191 cases

Our goal was to study the use of 2.0-mm miniplates for the fixation of mandibular fractures. Records of 191 patients who experienced a total of 280 mandibular fractures that were treated with 2.0-mm miniplates were reviewed. One hundred twelve of those patients, presenting 160 fractures, who attended a late follow-up were also clinically evaluated. Miniplates were used in the same positions described by AO/ASIF. No intermaxillary fixation was used. All patients included had a minimum follow-up of 6 months. Demographic data, procedures, postoperative results, and complications were analyzed. Mandibular fractures occurred mainly in males (mean age, 30.3 years). Mean follow-up was 21.92 months. The main etiology was motor vehicle accident. The most common fracture was the angle fracture (28.21%). Twenty-two fractures developed infection, for an overall incidence of 7.85%. When only angle fractures are considered, that incidence is increased to 18.98%. Although only 1 patient (0.89%) described inferior alveolar nerve paresthesia, objective testing revealed sensitivity alterations in 31.52% of the patients who had fractures in regions related to the inferior alveolar nerve. Temporary mild deficit of the marginal mandibular branch was observed in 2.56% of the extraoral approaches performed and 2.48% presented with hypertrophic scars. Incidence of occlusal alterations was 4.0%. Facial asymmetry was observed in 2.67% of the patients, whereas malunion incidence was 1.78%. Fibrous union, mostly partial, occurred in 2.38% of the fractures, but only 1 of those presented with mobility (0.59%). Condylar resorption developed in 6.25% of the fixated condylar fractures. Mean mouth opening was 42.08 mm. The overall incidence of complications, including infections, was similar to those described for more rigid methods of fixation.

Genomic analysis for managing small and endangered populations: a case study in Tyrol Grey cattle

Analysis of genomic data is increasingly becoming part of the livestock industry. Therefore, the routine collection of genomic information would be an invaluable resource for effective management of breeding programs in small, endangered populations. The objective of the paper was to demonstrate how genomic data could be used to analyse (1) linkage disequlibrium (LD), LD decay and the effective population size (NeLD); (2) Inbreeding level and effective population size (NeROH) based on runs of homozygosity (ROH); (3) Prediction of genomic breeding values (GEBV) using small within-breed and genomic information from other breeds. The Tyrol Grey population was used as an example, with the goal to highlight the potential of genomic analyses for small breeds. In addition to our own results we discuss additional use of genomics to assess relatedness, admixture proportions, and inheritance of harmful variants. The example data set consisted of 218 Tyrol Grey bull genotypes, which were all available AI bulls in the population. After standard quality control restrictions 34,581 SNPs remained for the analysis. A separate quality control was applied to determine ROH levels based on Illumina GenCall and Illumina GenTrain scores, resulting into 211 bulls and 33,604 SNPs. LD was computed as the squared correlation coefficient between SNPs within a 10 mega base pair (Mb) region. ROHs were derived based on regions covering at least 4, 8, and 16 Mb, suggesting that animals had common ancestors approximately 12, 6, and 3 generations ago, respectively. The corresponding mean inbreeding coefficients (F ROH) were 4.0% for 4 Mb, 2.9% for 8 Mb and 1.6% for 16 Mb runs. With an average generation interval of 5.66 years, estimated NeROH was 125 (NeROH>16 Mb), 186 (NeROH>8 Mb) and 370 (NeROH>4 Mb) indicating strict avoidance of close inbreeding in the population. The LD was used as an alternative method to infer the population history and the Ne. The results show a continuous decrease in NeLD, to 780, 120, and 80 for 100, 10, and 5 generations ago, respectively. Genomic selection was developed for and is working well in large breeds. The same methodology was applied in Tyrol Grey cattle, using different reference populations. Contrary to the expectations, the accuracy of GEBVs with very small within breed reference populations were very high, between 0.13-0.91 and 0.12-0.63, when estimated breeding values and deregressed breeding values were used as pseudo-phenotypes, respectively. Subsequent analyses confirmed the high accuracies being a consequence of low reliabilities of pseudo-phenotypes in the validation set, thus being heavily influenced by parent averages. Multi-breed and across breed reference sets gave inconsistent and lower accuracies. Genomic information may have a crucial role in management of small breeds, even if its primary usage differs from that of large breeds. It allows to assess relatedness between individuals, trends in inbreeding and to take decisions accordingly. These decisions would be based on the real genome architecture, rather than conventional pedigree information, which can be missing or incomplete. We strongly suggest the routine genotyping of all individuals that belong to a small breed in order to facilitate the effective management of endangered livestock populations.

Desenvolvimento de um bioprocesso utilizando-se resíduos para produção de amilases por Rhizopus oligosporus e etanol por Saccharomyces cerevisiae

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise dos riscos físicos e ergonômicos em roçadora transversal motorizada

According to Statistical Yearbook of Social Security (2011), the number of accidents at work recorded in the agricultural sector is quite high, in the year 2010 there are records of thirty-four thousand nine hundred and ninetysix accidents, and in the year of 2011 there were thirty-one thousand and ninety-six accidents in the industry. Among so many diseases and accidents at work hearing loss is one of them. According to the Pan American Health Organization (2011) the Occupational hearing loss is then jury to the worker's health, often in the workplace. Mechanical vibrations are also present in many human activities exposing employees to an extremely aggressive agent. The combined action of these two factors can cause damage to the health of the worker, in this way, noise and vibration are the two major occupational agents present in the brush cutter operator activity. In the case there is another risk factor, the open work, which exposes the employee to the Sun’s rays and the high temperature indices. Studies have shown that: noise exceeds 115 dB (A) vibration 4.0 m/s2 and thermal stress of 29.9 o C. Ergonomic evaluations also demonstrated that the activity cause injury to workers.