Underway water measurements of halocarbons during POSEIDON cruise POS399 in June 2010

Methyl iodide (CH3I), bromoform (CHBr3) and dibromomethane (CH2Br2), which are produced naturally in the oceans, take part in ozone chemistry both in the troposphere and the stratosphere. The significance of oceanic upwelling regions for emissions of these trace gases in the global context is still uncertain although they have been identified as important source regions. To better quantify the role of upwelling areas in current and future climate, this paper analyzes major factors that influenced halocarbon emissions from the tropical North East Atlantic including the Mauritanian upwelling during the DRIVE expedition. Diel and regional variability of oceanic and atmospheric CH3I, CHBr3 and CH2Br2 was determined along with biological and meteorological parameters at six 24 h-stations. Low oceanic concentrations of CH3I from 0.1-5.4 pmol/L were equally distributed throughout the investigation area. CHBr3 of 1.0-42.4 pmol/L and CH2Br2 of 1.0-9.4 pmol/L were measured with maximum concentrations close to the Mauritanian coast. Atmospheric mixing rations of CH3I of up to 3.3, CHBr3 to 8.9 and CH2Br2 to 3.1 ppt above the upwelling and 1.8, 12.8, respectively 2.2 ppt at a Cape Verdean coast were detected during the campaign. While diel variability in CH3I emissions could be mainly ascribed to oceanic non-biological production, no main driver was identified for its emissions in the entire study region. In contrast, oceanic bromocarbons resulted from biogenic sources which were identified as regional drivers of their sea-to-air fluxes. The diel impact of wind speed on bromocarbon emissions increased with decreasing distance to the coast. The height of the marine atmospheric boundary layer (MABL) was determined as an additional factor influencing halocarbon emissions. Oceanic and atmospheric halocarbons correlated well in the study region and in combination with high oceanic CH3I, CHBr3 and CH2Br2 concentrations, local hot spots of atmospheric halocarbons could solely be explained by marine sources. This conclusion is in contrast with previous studies that hypothesized the occurrence of elevated atmospheric halocarbons over the eastern tropical Atlantic mainly originating from the West-African continent.

Rare-earth elements and isotope ratios at DSDP Leg 82 Holes

We report 48 analyses of rare-earth elements (REE) and 15 143Nd/144Nd and 87Sr/86Sr analyses for basalts from the eight holes drilled during Leg 82. Discrete and distinct REE patterns and 143Nd/144Nd ratios characterize the eight holes, with little variation observed downhole except in Holes 561 and 558, thus suggesting dominantly long-term temporal and large-scale spatial variations in the mantle source of these basalts beneath the Mid-Atlantic Ridge over the last 35 Ma of its spreading activity. There is a good inverse correlation between 143Nd/144Nd and (La/Sm)EF with one exception in Hole 558 (approximately 35 Ma), the latter suggesting a recent (35 Ma) light REE depletion event, perhaps caused by dynamic or fractional melting. Short-term temporal and small-scale spatial mantle source variability is also evident in Hole 561 (approximately 18 Ma), which has rapid fluctuations in REE patterns and 143Nd/144Nd ratios (suggesting rapid transfer of magma from the time of melting) and is evidence contrary to the presence of a well-mixed magma chamber at this particular site and time. The mantle source variations noted can be interpreted within two extreme models. The first model invokes a convecting mantle depleted in large ion lithophile elements (LILE) and containing lumps (or veins) of LILE-enriched material of various shapes and sizes, passively and randomly distributed throughout. A second more restrictive model considers the interaction of fixed mantle plumes and the LILE-depleted asthenosphere flowing towards a migrating Mid- Atlantic Ridge (MAR) axis. With the exception of Hole 558 and the uncertainties of reconstructions of absolute plate movements in the region, the observed variations can be explained by two hot spots; the nearly ridge-centered Azores hot spot (plume) and another hot spot located beneath the African plate that may be affecting the source of basalts currently erupting at the MAR axis at 35°N and which, in the past, would have produced the New England chain of seamounts on the North American plate and (later) the Atlantis-Great Meteor chain on the African plate. Basalts erupted south of the Hayes Fracture Zone have not been affected by either of these two hot spots over the last 35 Ma and appear to have been continuously derived from the LILE-depleted source. Subaxial flow downridge from the Azores plume appears to have started 9 Ma, on the basis of the southward converging V-shaped time-transgressive ridges branching from the Pico and Corves Island, or not earlier than 16 Ma, on the basis of the geochemical results. Variations within Hole 558 remains unexplained by the latter model, unless we hypothesize a third hot spot.

On-Chip Thermal Monitoring: Design, Placement and Interconnection of Temperature Sensors

La temperatura es una preocupación que juega un papel protagonista en el diseño de circuitos integrados modernos. El importante aumento de las densidades de potencia que conllevan las últimas generaciones tecnológicas ha producido la aparición de gradientes térmicos y puntos calientes durante el funcionamiento normal de los chips. La temperatura tiene un impacto negativo en varios parámetros del circuito integrado como el retardo de las puertas, los gastos de disipación de calor, la fiabilidad, el consumo de energía, etc. Con el fin de luchar contra estos efectos nocivos, la técnicas de gestión dinámica de la temperatura (DTM) adaptan el comportamiento del chip en función en la información que proporciona un sistema de monitorización que mide en tiempo de ejecución la información térmica de la superficie del dado. El campo de la monitorización de la temperatura en el chip ha llamado la atención de la comunidad científica en los últimos años y es el objeto de estudio de esta tesis. Esta tesis aborda la temática de control de la temperatura en el chip desde diferentes perspectivas y niveles, ofreciendo soluciones a algunos de los temas más importantes. Los niveles físico y circuital se cubren con el diseño y la caracterización de dos nuevos sensores de temperatura especialmente diseñados para los propósitos de las técnicas DTM. El primer sensor está basado en un mecanismo que obtiene un pulso de anchura variable dependiente de la relación de las corrientes de fuga con la temperatura. De manera resumida, se carga un nodo del circuito y posteriormente se deja flotando de tal manera que se descarga a través de las corrientes de fugas de un transistor; el tiempo de descarga del nodo es la anchura del pulso. Dado que la anchura del pulso muestra una dependencia exponencial con la temperatura, la conversión a una palabra digital se realiza por medio de un contador logarítmico que realiza tanto la conversión tiempo a digital como la linealización de la salida. La estructura resultante de esta combinación de elementos se implementa en una tecnología de 0,35 _m. El sensor ocupa un área muy reducida, 10.250 nm2, y consume muy poca energía, 1.05-65.5nW a 5 muestras/s, estas cifras superaron todos los trabajos previos en el momento en que se publicó por primera vez y en el momento de la publicación de esta tesis, superan a todas las implementaciones anteriores fabricadas en el mismo nodo tecnológico. En cuanto a la precisión, el sensor ofrece una buena linealidad, incluso sin calibrar; se obtiene un error 3_ de 1,97oC, adecuado para tratar con las aplicaciones de DTM. Como se ha explicado, el sensor es completamente compatible con los procesos de fabricación CMOS, este hecho, junto con sus valores reducidos de área y consumo, lo hacen especialmente adecuado para la integración en un sistema de monitorización de DTM con un conjunto de monitores empotrados distribuidos a través del chip. Las crecientes incertidumbres de proceso asociadas a los últimos nodos tecnológicos comprometen las características de linealidad de nuestra primera propuesta de sensor. Con el objetivo de superar estos problemas, proponemos una nueva técnica para obtener la temperatura. La nueva técnica también está basada en las dependencias térmicas de las corrientes de fuga que se utilizan para descargar un nodo flotante. La novedad es que ahora la medida viene dada por el cociente de dos medidas diferentes, en una de las cuales se altera una característica del transistor de descarga |la tensión de puerta. Este cociente resulta ser muy robusto frente a variaciones de proceso y, además, la linealidad obtenida cumple ampliamente los requisitos impuestos por las políticas DTM |error 3_ de 1,17oC considerando variaciones del proceso y calibrando en dos puntos. La implementación de la parte sensora de esta nueva técnica implica varias consideraciones de diseño, tales como la generación de una referencia de tensión independiente de variaciones de proceso, que se analizan en profundidad en la tesis. Para la conversión tiempo-a-digital, se emplea la misma estructura de digitalización que en el primer sensor. Para la implementación física de la parte de digitalización, se ha construido una biblioteca de células estándar completamente nueva orientada a la reducción de área y consumo. El sensor resultante de la unión de todos los bloques se caracteriza por una energía por muestra ultra baja (48-640 pJ) y un área diminuta de 0,0016 mm2, esta cifra mejora todos los trabajos previos. Para probar esta afirmación, se realiza una comparación exhaustiva con más de 40 propuestas de sensores en la literatura científica. Subiendo el nivel de abstracción al sistema, la tercera contribución se centra en el modelado de un sistema de monitorización que consiste de un conjunto de sensores distribuidos por la superficie del chip. Todos los trabajos anteriores de la literatura tienen como objetivo maximizar la precisión del sistema con el mínimo número de monitores. Como novedad, en nuestra propuesta se introducen nuevos parámetros de calidad aparte del número de sensores, también se considera el consumo de energía, la frecuencia de muestreo, los costes de interconexión y la posibilidad de elegir diferentes tipos de monitores. El modelo se introduce en un algoritmo de recocido simulado que recibe la información térmica de un sistema, sus propiedades físicas, limitaciones de área, potencia e interconexión y una colección de tipos de monitor; el algoritmo proporciona el tipo seleccionado de monitor, el número de monitores, su posición y la velocidad de muestreo _optima. Para probar la validez del algoritmo, se presentan varios casos de estudio para el procesador Alpha 21364 considerando distintas restricciones. En comparación con otros trabajos previos en la literatura, el modelo que aquí se presenta es el más completo. Finalmente, la última contribución se dirige al nivel de red, partiendo de un conjunto de monitores de temperatura de posiciones conocidas, nos concentramos en resolver el problema de la conexión de los sensores de una forma eficiente en área y consumo. Nuestra primera propuesta en este campo es la introducción de un nuevo nivel en la jerarquía de interconexión, el nivel de trillado (o threshing en inglés), entre los monitores y los buses tradicionales de periféricos. En este nuevo nivel se aplica selectividad de datos para reducir la cantidad de información que se envía al controlador central. La idea detrás de este nuevo nivel es que en este tipo de redes la mayoría de los datos es inútil, porque desde el punto de vista del controlador sólo una pequeña cantidad de datos |normalmente sólo los valores extremos| es de interés. Para cubrir el nuevo nivel, proponemos una red de monitorización mono-conexión que se basa en un esquema de señalización en el dominio de tiempo. Este esquema reduce significativamente tanto la actividad de conmutación sobre la conexión como el consumo de energía de la red. Otra ventaja de este esquema es que los datos de los monitores llegan directamente ordenados al controlador. Si este tipo de señalización se aplica a sensores que realizan conversión tiempo-a-digital, se puede obtener compartición de recursos de digitalización tanto en tiempo como en espacio, lo que supone un importante ahorro de área y consumo. Finalmente, se presentan dos prototipos de sistemas de monitorización completos que de manera significativa superan la características de trabajos anteriores en términos de área y, especialmente, consumo de energía. Abstract Temperature is a first class design concern in modern integrated circuits. The important increase in power densities associated to recent technology evolutions has lead to the apparition of thermal gradients and hot spots during run time operation. Temperature impacts several circuit parameters such as speed, cooling budgets, reliability, power consumption, etc. In order to fight against these negative effects, dynamic thermal management (DTM) techniques adapt the behavior of the chip relying on the information of a monitoring system that provides run-time thermal information of the die surface. The field of on-chip temperature monitoring has drawn the attention of the scientific community in the recent years and is the object of study of this thesis. This thesis approaches the matter of on-chip temperature monitoring from different perspectives and levels, providing solutions to some of the most important issues. The physical and circuital levels are covered with the design and characterization of two novel temperature sensors specially tailored for DTM purposes. The first sensor is based upon a mechanism that obtains a pulse with a varying width based on the variations of the leakage currents on the temperature. In a nutshell, a circuit node is charged and subsequently left floating so that it discharges away through the subthreshold currents of a transistor; the time the node takes to discharge is the width of the pulse. Since the width of the pulse displays an exponential dependence on the temperature, the conversion into a digital word is realized by means of a logarithmic counter that performs both the timeto- digital conversion and the linearization of the output. The structure resulting from this combination of elements is implemented in a 0.35_m technology and is characterized by very reduced area, 10250 nm2, and power consumption, 1.05-65.5 nW at 5 samples/s, these figures outperformed all previous works by the time it was first published and still, by the time of the publication of this thesis, they outnumber all previous implementations in the same technology node. Concerning the accuracy, the sensor exhibits good linearity, even without calibration it displays a 3_ error of 1.97oC, appropriate to deal with DTM applications. As explained, the sensor is completely compatible with standard CMOS processes, this fact, along with its tiny area and power overhead, makes it specially suitable for the integration in a DTM monitoring system with a collection of on-chip monitors distributed across the chip. The exacerbated process fluctuations carried along with recent technology nodes jeop-ardize the linearity characteristics of the first sensor. In order to overcome these problems, a new temperature inferring technique is proposed. In this case, we also rely on the thermal dependencies of leakage currents that are used to discharge a floating node, but now, the result comes from the ratio of two different measures, in one of which we alter a characteristic of the discharging transistor |the gate voltage. This ratio proves to be very robust against process variations and displays a more than suficient linearity on the temperature |1.17oC 3_ error considering process variations and performing two-point calibration. The implementation of the sensing part based on this new technique implies several issues, such as the generation of process variations independent voltage reference, that are analyzed in depth in the thesis. In order to perform the time-to-digital conversion, we employ the same digitization structure the former sensor used. A completely new standard cell library targeting low area and power overhead is built from scratch to implement the digitization part. Putting all the pieces together, we achieve a complete sensor system that is characterized by ultra low energy per conversion of 48-640pJ and area of 0.0016mm2, this figure outperforms all previous works. To prove this statement, we perform a thorough comparison with over 40 works from the scientific literature. Moving up to the system level, the third contribution is centered on the modeling of a monitoring system consisting of set of thermal sensors distributed across the chip. All previous works from the literature target maximizing the accuracy of the system with the minimum number of monitors. In contrast, we introduce new metrics of quality apart form just the number of sensors; we consider the power consumption, the sampling frequency, the possibility to consider different types of monitors and the interconnection costs. The model is introduced in a simulated annealing algorithm that receives the thermal information of a system, its physical properties, area, power and interconnection constraints and a collection of monitor types; the algorithm yields the selected type of monitor, the number of monitors, their position and the optimum sampling rate. We test the algorithm with the Alpha 21364 processor under several constraint configurations to prove its validity. When compared to other previous works in the literature, the modeling presented here is the most complete. Finally, the last contribution targets the networking level, given an allocated set of temperature monitors, we focused on solving the problem of connecting them in an efficient way from the area and power perspectives. Our first proposal in this area is the introduction of a new interconnection hierarchy level, the threshing level, in between the monitors and the traditional peripheral buses that applies data selectivity to reduce the amount of information that is sent to the central controller. The idea behind this new level is that in this kind of networks most data are useless because from the controller viewpoint just a small amount of data |normally extreme values| is of interest. To cover the new interconnection level, we propose a single-wire monitoring network based on a time-domain signaling scheme that significantly reduces both the switching activity over the wire and the power consumption of the network. This scheme codes the information in the time domain and allows a straightforward obtention of an ordered list of values from the maximum to the minimum. If the scheme is applied to monitors that employ TDC, digitization resource sharing is achieved, producing an important saving in area and power consumption. Two prototypes of complete monitoring systems are presented, they significantly overcome previous works in terms of area and, specially, power consumption.

Distributed control strategies for photovoltaic systems in urban environments

Este trabajo es una contribución a los sistemas fotovoltaicos (FV) con seguimiento distribuido del punto de máxima potencia (DMPPT), una topología que se caracteriza porque lleva a cabo el MPPT a nivel de módulo, al contrario de las topologías más tradicionales que llevan a cabo el MPPT para un número más elevado de módulos, pudiendo ser hasta cientos de módulos. Las dos tecnologías DMPPT que existen en el mercado son conocidos como microinversores y optimizadores de potencia, y ofrecen ciertas ventajas sobre sistemas de MPPT central como: mayor producción en situaciones de mismatch, monitorización individual de cada módulo, flexibilidad de diseño, mayor seguridad del sistema, etc. Aunque los sistemas DMPPT no están limitados a los entornos urbanos, se ha enfatizado en el título ya que es su mercado natural, siendo difícil una justificación de su sobrecoste en grandes huertas solares en suelo. Desde el año 2010 el mercado de estos sistemas ha incrementado notablemente y sigue creciendo de una forma continuada. Sin embargo, todavía falta un conocimiento profundo de cómo funcionan estos sistemas, especialmente en el caso de los optimizadores de potencia, de las ganancias energéticas esperables en condiciones de mismatch y de las posibilidades avanzadas de diagnóstico de fallos. El principal objetivo de esta tesis es presentar un estudio completo de cómo funcionan los sistemas DMPPT, sus límites y sus ventajas, así como experimentos varios que verifican la teoría y el desarrollo de herramientas para valorar las ventajas de utilizar DMPPT en cada instalación. Las ecuaciones que modelan el funcionamiento de los sistemas FVs con optimizadores de potencia se han desarrollado y utilizado para resaltar los límites de los mismos a la hora de resolver ciertas situaciones de mismatch. Se presenta un estudio profundo sobre el efecto de las sombras en los sistemas FVs: en la curva I-V y en los algoritmos MPPT. Se han llevado a cabo experimentos sobre el funcionamiento de los algoritmos MPPT en situaciones de sombreado, señalando su ineficiencia en estas situaciones. Un análisis de la ventaja del uso de DMPPT frente a los puntos calientes es presentado y verificado. También se presenta un análisis sobre las posibles ganancias en potencia y energía con el uso de DMPPT en condiciones de sombreado y este también es verificado experimentalmente, así como un breve estudio de su viabilidad económica. Para ayudar a llevar a cabo todos los análisis y experimentos descritos previamente se han desarrollado una serie de herramientas software. Una siendo un programa en LabView para controlar un simulador solar y almacenar las medidas. También se ha desarrollado un programa que simula curvas I-V de módulos y generador FVs afectados por sombras y este se ha verificado experimentalmente. Este mismo programa se ha utilizado para desarrollar un programa todavía más completo que estima las pérdidas anuales y las ganancias obtenidas con DMPPT en instalaciones FVs afectadas por sombras. Finalmente, se han desarrollado y verificado unos algoritmos para diagnosticar fallos en sistemas FVs con DMPPT. Esta herramienta puede diagnosticar los siguientes fallos: sombras debido a objetos fijos (con estimación de la distancia al objeto), suciedad localizada, suciedad general, posible punto caliente, degradación de módulos y pérdidas en el cableado de DC. Además, alerta al usuario de las pérdidas producidas por cada fallo y no requiere del uso de sensores de irradiancia y temperatura. ABSTRACT This work is a contribution to photovoltaic (PV) systems with distributed maximum power point tracking (DMPPT), a system topology characterized by performing the MPPT at module level, instead of the more traditional topologies which perform MPPT for a larger number of modules. The two DMPPT technologies available at the moment are known as microinverters and power optimizers, also known as module level power electronics (MLPE), and they provide certain advantages over central MPPT systems like: higher energy production in mismatch situations, monitoring of each individual module, system design flexibility, higher system safety, etc. Although DMPPT is not limited to urban environments, it has been emphasized in the title as it is their natural market, since in large ground-mounted PV plants the extra cost is difficult to justify. Since 2010 MLPE have increased their market share steadily and continuing to grow steadily. However, there still lacks a profound understanding of how they work, especially in the case of power optimizers, the achievable energy gains with their use and the possibilities in failure diagnosis. The main objective of this thesis is to provide a complete understanding of DMPPT technologies: how they function, their limitations and their advantages. A series of equations used to model PV arrays with power optimizers have been derived and used to point out limitations in solving certain mismatch situation. Because one of the most emphasized benefits of DMPPT is their ability to mitigate shading losses, an extensive study on the effects of shadows on PV systems is presented; both on the I-V curve and on MPPT algorithms. Experimental tests have been performed on the MPPT algorithms of central inverters and MLPE, highlighting their inefficiency in I-V curves with local maxima. An analysis of the possible mitigation of hot-spots with DMPPT is discussed and experimentally verified. And a theoretical analysis of the possible power and energy gains is presented as well as experiments in real PV systems. A short economic analysis of the benefits of DMPPT has also been performed. In order to aide in the previous task, a program which simulates I-V curves under shaded conditions has been developed and experimentally verified. This same program has been used to develop a software tool especially designed for PV systems affected by shading, which estimates the losses due to shading and the energy gains obtained with DMPPT. Finally, a set of algorithms for diagnosing system faults in PV systems with DMPPT has been developed and experimentally verified. The tool can diagnose the following failures: fixed object shading (with distance estimation), localized dirt, generalized dirt, possible hot-spots, module degradation and excessive losses in DC cables. In addition, it alerts the user of the power losses produced by each failure and classifies the failures by their severity and it does not require the use of irradiance or temperature sensors.

Distributed control strategies for photovoltaic systems in urban environments : Versión definitiva

Este trabajo es una contribución a los sistemas fotovoltaicos (FV) con seguimiento distribuido del punto de máxima potencia (DMPPT), una topología que se caracteriza porque lleva a cabo el MPPT a nivel de módulo, al contrario de las topologías más tradicionales que llevan a cabo el MPPT para un número más elevado de módulos, pudiendo ser hasta cientos de módulos. Las dos tecnologías DMPPT que existen en el mercado son conocidos como microinversores y optimizadores de potencia, y ofrecen ciertas ventajas sobre sistemas de MPPT central como: mayor producción en situaciones de mismatch, monitorización individual de cada módulo, flexibilidad de diseño, mayor seguridad del sistema, etc. Aunque los sistemas DMPPT no están limitados a los entornos urbanos, se ha enfatizado en el título ya que es su mercado natural, siendo difícil una justificación de su sobrecoste en grandes huertas solares en suelo. Desde el año 2010 el mercado de estos sistemas ha incrementado notablemente y sigue creciendo de una forma continuada. Sin embargo, todavía falta un conocimiento profundo de cómo funcionan estos sistemas, especialmente en el caso de los optimizadores de potencia, de las ganancias energéticas esperables en condiciones de mismatch y de las posibilidades avanzadas de diagnóstico de fallos. El principal objetivo de esta tesis es presentar un estudio completo de cómo funcionan los sistemas DMPPT, sus límites y sus ventajas, así como experimentos varios que verifican la teoría y el desarrollo de herramientas para valorar las ventajas de utilizar DMPPT en cada instalación. Las ecuaciones que modelan el funcionamiento de los sistemas FVs con optimizadores de potencia se han desarrollado y utilizado para resaltar los límites de los mismos a la hora de resolver ciertas situaciones de mismatch. Se presenta un estudio profundo sobre el efecto de las sombras en los sistemas FVs: en la curva I-V y en los algoritmos MPPT. Se han llevado a cabo experimentos sobre el funcionamiento de los algoritmos MPPT en situaciones de sombreado, señalando su ineficiencia en estas situaciones. Un análisis de la ventaja del uso de DMPPT frente a los puntos calientes es presentado y verificado. También se presenta un análisis sobre las posibles ganancias en potencia y energía con el uso de DMPPT en condiciones de sombreado y este también es verificado experimentalmente, así como un breve estudio de su viabilidad económica. Para ayudar a llevar a cabo todos los análisis y experimentos descritos previamente se han desarrollado una serie de herramientas software. Una siendo un programa en LabView para controlar un simulador solar y almacenar las medidas. También se ha desarrollado un programa que simula curvas I-V de módulos y generador FVs afectados por sombras y este se ha verificado experimentalmente. Este mismo programa se ha utilizado para desarrollar un programa todavía más completo que estima las pérdidas anuales y las ganancias obtenidas con DMPPT en instalaciones FVs afectadas por sombras. Finalmente, se han desarrollado y verificado unos algoritmos para diagnosticar fallos en sistemas FVs con DMPPT. Esta herramienta puede diagnosticar los siguientes fallos: sombras debido a objetos fijos (con estimación de la distancia al objeto), suciedad localizada, suciedad general, posible punto caliente, degradación de módulos y pérdidas en el cableado de DC. Además, alerta al usuario de las pérdidas producidas por cada fallo y no requiere del uso de sensores de irradiancia y temperatura. ABSTRACT This work is a contribution to photovoltaic (PV) systems with distributed maximum power point tracking (DMPPT), a system topology characterized by performing the MPPT at module level, instead of the more traditional topologies which perform MPPT for a larger number of modules. The two DMPPT technologies available at the moment are known as microinverters and power optimizers, also known as module level power electronics (MLPE), and they provide certain advantages over central MPPT systems like: higher energy production in mismatch situations, monitoring of each individual module, system design flexibility, higher system safety, etc. Although DMPPT is not limited to urban environments, it has been emphasized in the title as it is their natural market, since in large ground-mounted PV plants the extra cost is difficult to justify. Since 2010 MLPE have increased their market share steadily and continuing to grow steadily. However, there still lacks a profound understanding of how they work, especially in the case of power optimizers, the achievable energy gains with their use and the possibilities in failure diagnosis. The main objective of this thesis is to provide a complete understanding of DMPPT technologies: how they function, their limitations and their advantages. A series of equations used to model PV arrays with power optimizers have been derived and used to point out limitations in solving certain mismatch situation. Because one of the most emphasized benefits of DMPPT is their ability to mitigate shading losses, an extensive study on the effects of shadows on PV systems is presented; both on the I-V curve and on MPPT algorithms. Experimental tests have been performed on the MPPT algorithms of central inverters and MLPE, highlighting their inefficiency in I-V curves with local maxima. An analysis of the possible mitigation of hot-spots with DMPPT is discussed and experimentally verified. And a theoretical analysis of the possible power and energy gains is presented as well as experiments in real PV systems. A short economic analysis of the benefits of DMPPT has also been performed. In order to aide in the previous task, a program which simulates I-V curves under shaded conditions has been developed and experimentally verified. This same program has been used to develop a software tool especially designed for PV systems affected by shading, which estimates the losses due to shading and the energy gains obtained with DMPPT. Finally, a set of algorithms for diagnosing system faults in PV systems with DMPPT has been developed and experimentally verified. The tool can diagnose the following failures: fixed object shading (with distance estimation), localized dirt, generalized dirt, possible hot-spots, module degradation and excessive losses in DC cables. In addition, it alerts the user of the power losses produced by each failure and classifies the failures by their severity and it does not require the use of irradiance or temperature sensors.

Modelling agro-forestry scenarios for ammonia abatement in the landscape

Ammonia emissions from livestock production can have negative impacts on nearby protected sites and ecosystems that are sensitive to eutrophication and acidification. Trees are effective scavengers of both gaseous and particulate pollutants from the atmosphere making tree belts potentially effective landscape features to support strategies aiming to reduce ammonia impacts. This research used the MODDAS-THETIS a coupled turbulence and deposition turbulence model, to examine the relationships between tree canopy structure and ammonia capture for three source types?animal housing, slurry lagoon, and livestock under a tree canopy. By altering the canopy length, leaf area index, leaf area density, and height of the canopy in the model the capture efficiencies varied substantially. A maximum of 27% of the emitted ammonia was captured by tree canopy for the animal housing source, for the slurry lagoon the maximum was 19%, while the livestock under trees attained a maximum of 60% recapture. Using agro-forestry systems of differing tree structures near ?hot spots? of ammonia in the landscape could provide an effective abatement option for the livestock industry that complements existing source reduction measures.

Contributions to uncertainty reduction in the estimation of PV plants performance

Esta tesis doctoral presenta un procedimiento integral de control de calidad en centrales fotovoltaicas, que comprende desde la fase inicial de estimación de las expectativas de producción hasta la vigilancia del funcionamiento de la instalación una vez en operación, y que permite reducir la incertidumbre asociada su comportamiento y aumentar su fiabilidad a largo plazo, optimizando su funcionamiento. La coyuntura de la tecnología fotovoltaica ha evolucionado enormemente en los últimos años, haciendo que las centrales fotovoltaicas sean capaces de producir energía a unos precios totalmente competitivos en relación con otras fuentes de energía. Esto hace que aumente la exigencia sobre el funcionamiento y la fiabilidad de estas instalaciones. Para cumplir con dicha exigencia, es necesaria la adecuación de los procedimientos de control de calidad aplicados, así como el desarrollo de nuevos métodos que deriven en un conocimiento más completo del estado de las centrales, y que permitan mantener la vigilancia sobre las mismas a lo largo del tiempo. Además, los ajustados márgenes de explotación actuales requieren que durante la fase de diseño se disponga de métodos de estimación de la producción que comporten la menor incertidumbre posible. La propuesta de control de calidad presentada en este trabajo parte de protocolos anteriores orientados a la fase de puesta en marcha de una instalación fotovoltaica, y las complementa con métodos aplicables a la fase de operación, prestando especial atención a los principales problemas que aparecen en las centrales a lo largo de su vida útil (puntos calientes, impacto de la suciedad, envejecimiento…). Además, incorpora un protocolo de vigilancia y análisis del funcionamiento de las instalaciones a partir de sus datos de monitorización, que incluye desde la comprobación de la validez de los propios datos registrados hasta la detección y el diagnóstico de fallos, y que permite un conocimiento automatizado y detallado de las plantas. Dicho procedimiento está orientado a facilitar las tareas de operación y mantenimiento, de manera que se garantice una alta disponibilidad de funcionamiento de la instalación. De vuelta a la fase inicial de cálculo de las expectativas de producción, se utilizan los datos registrados en las centrales para llevar a cabo una mejora de los métodos de estimación de la radiación, que es la componente que más incertidumbre añade al proceso de modelado. El desarrollo y la aplicación de este procedimiento de control de calidad se han llevado a cabo en 39 grandes centrales fotovoltaicas, que totalizan una potencia de 250 MW, distribuidas por varios países de Europa y América Latina. ABSTRACT This thesis presents a comprehensive quality control procedure to be applied in photovoltaic plants, which covers from the initial phase of energy production estimation to the monitoring of the installation performance, once it is in operation. This protocol allows reducing the uncertainty associated to the photovoltaic plants behaviour and increases their long term reliability, therefore optimizing their performance. The situation of photovoltaic technology has drastically evolved in recent years, making photovoltaic plants capable of producing energy at fully competitive prices, in relation to other energy sources. This fact increases the requirements on the performance and reliability of these facilities. To meet this demand, it is necessary to adapt the quality control procedures and to develop new methods able to provide a more complete knowledge of the state of health of the plants, and able to maintain surveillance on them over time. In addition, the current meagre margins in which these installations operate require procedures capable of estimating energy production with the lower possible uncertainty during the design phase. The quality control procedure presented in this work starts from previous protocols oriented to the commissioning phase of a photovoltaic system, and complete them with procedures for the operation phase, paying particular attention to the major problems that arise in photovoltaic plants during their lifetime (hot spots, dust impact, ageing...). It also incorporates a protocol to control and analyse the installation performance directly from its monitoring data, which comprises from checking the validity of the recorded data itself to the detection and diagnosis of failures, and which allows an automated and detailed knowledge of the PV plant performance that can be oriented to facilitate the operation and maintenance of the installation, so as to ensure a high operation availability of the system. Back to the initial stage of calculating production expectations, the data recorded in the photovoltaic plants is used to improved methods for estimating the incident irradiation, which is the component that adds more uncertainty to the modelling process. The development and implementation of the presented quality control procedure has been carried out in 39 large photovoltaic plants, with a total power of 250 MW, located in different European and Latin-American countries.

The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations

The Saccharomyces cerevisiae genome encodes four MutL homologs. Of these, MLH1 and PMS1 are known to act in the MSH2-dependent pathway that repairs DNA mismatches. We have investigated the role of MLH3 in mismatch repair. Mutations in MLH3 increased the rate of reversion of the hom3–10 allele by increasing the rate of deletion of a single T in a run of 7 Ts. Combination of mutations in MLH3 and MSH6 caused a synergistic increase in the hom3–10 reversion rate, whereas the hom3–10 reversion rate in an mlh3 msh3 double mutant was the same as in the respective single mutants. Similar results were observed when the accumulation of mutations at frameshift hot spots in the LYS2 gene was analyzed, although mutation of MLH3 did not cause the same extent of affect at every LYS2 frameshift hot spot. MLH3 interacted with MLH1 in a two-hybrid system. These data are consistent with the idea that a proportion of the repair of specific insertion/deletion mispairs by the MSH3-dependent mismatch repair pathway uses a heterodimeric MLH1-MLH3 complex in place of the MLH1-PMS1 complex.

Vascular endothelial growth factor: Crystal structure and functional mapping of the kinase domain receptor binding site

Vascular endothelial growth factor (VEGF) is a homodimeric member of the cystine knot family of growth factors, with limited sequence homology to platelet-derived growth factor (PDGF) and transforming growth factor β2 (TGF-β). We have determined its crystal structure at a resolution of 2.5 Å, and identified its kinase domain receptor (KDR) binding site using mutational analysis. Overall, the VEGF monomer resembles that of PDGF, but its N-terminal segment is helical rather than extended. The dimerization mode of VEGF is similar to that of PDGF and very different from that of TGF-β. Mutational analysis of VEGF reveals that symmetrical binding sites for KDR are located at each pole of the VEGF homodimer. Each site contains two functional “hot spots” composed of binding determinants presented across the subunit interface. The two most important determinants are located within the largest hot spot on a short, three-stranded sheet that is conserved in PDGF and TGF-β. Functional analysis of the binding epitopes for two receptor-blocking antibodies reveal different binding determinants near each of the KDR binding hot spots.

Sequence of the FRA3B common fragile region: Implications for the mechanism of FHIT deletion

The hypothesis that chromosomal fragile sites may be “weak links” that result in hot spots for cancer-specific chromosome rearrangements was supported by the discovery that numerous cancer cell homozygous deletions and a familial translocation map within the FHIT gene, which encompasses the common fragile site, FRA3B. Sequence analysis of 276 kb of the FRA3B/FHIT locus and 22 associated cancer cell deletion endpoints shows that this locus is a frequent target of homologous recombination between long interspersed nuclear element sequences resulting in FHIT gene internal deletions, probably as a result of carcinogen-induced damage at FRA3B fragile sites.

Activation-dependent changes in receptor distribution and dendritic morphology in hippocampal neurons expressing P2X2-green fluorescent protein receptors

ATP-gated P2X2 receptors are widely expressed in neurons, but the cellular effects of receptor activation are unclear. We engineered functional green fluorescent protein (GFP)-tagged P2X2 receptors and expressed them in embryonic hippocampal neurons, and report an approach to determining functional and total receptor pool sizes in living cells. ATP application to dendrites caused receptor redistribution and the formation of varicose hot spots of higher P2X2-GFP receptor density. Redistribution in dendrites was accompanied by an activation-dependent enhancement of the ATP-evoked current. Substate-specific mutant T18A P2X2-GFP receptors showed no redistribution or activation-dependent enhancement of the ATP-evoked current. Thus fluorescent P2X2-GFP receptors function normally, can be quantified, and reveal the dynamics of P2X2 receptor distribution on the seconds time scale.

Expression of error-prone polymerases in BL2 cells activated for Ig somatic hypermutation

High affinity antibodies are generated in mice and humans by means of somatic hypermutation (SHM) of variable (V) regions of Ig genes. Mutations with rates of 10−5–10−3 per base pair per generation, about 106-fold above normal, are targeted primarily at V-region hot spots by unknown mechanisms. We have measured mRNA expression of DNA polymerases ι, η, and ζ by using cultured Burkitt's lymphoma (BL)2 cells. These cells exhibit 5–10-fold increases in heavy-chain V-region mutations targeted only predominantly to RGYW (R = A or G, Y = C or T, W = T or A) hot spots if costimulated with T cells and IgM crosslinking, the presumed in vivo requirements for SHM. An ∼4-fold increase pol ι mRNA occurs within 12 h when cocultured with T cells and surface IgM crosslinking. Induction of pols η and ζ occur with T cells, IgM crosslinking, or both stimuli. The fidelity of pol ι was measured at RGYW hot- and non-hot-spot sequences situated at nicks, gaps, and double-strand breaks. Pol ι formed T⋅G mispairs at a frequency of 10−2, consistent with SHM-generated C to T transitions, with a 3-fold increased error rate in hot- vs. non-hot-spot sequences for the single-nucleotide overhang. The T cell and IgM crosslinking-dependent induction of pol ι at 12 h may indicate an SHM “triggering” event has occurred. However, pols ι, η, and ζ are present under all conditions, suggesting that their presence is not sufficient to generate mutations because both T cell and IgM stimuli are required for SHM induction.

On the existence and implications of an inverse folding code in proteins.

The existence of a code relating the set of possible sequences at a given position in a protein backbone to the local structure at that location is investigated. It is shown that only 73% of 4-C alpha structure fragments in a sample of 114 protein structures exhibit a preference for a particular set of sequences. The remaining structures can accommodate essentially any sequence. The structures that encode specific sequence distributions include the classical "secondary" structures, with the notable exception of planar (beta) bends. It is suggested that this has implications as to the mechanism of folding in proteins with extensive sheet/barrel structure. The possible role of structures that do not encode specific sequences as mutation hot spots is noted.

Geochemistry of surface sediment samples from the western Barents Sea region

Here, we present bulk organic geochemical data from a spatial grid of surface samples from the western Barents Sea region. The results show that the distribution of organic carbon in surface sediments is predominantly controlled by input from land-derived terrigenous and in-situ produced marine organic matter. Inferred from various nitrogenous fractions and stable isotopes of bulk organic carbon we show that the spatial distribution of terrigenous organic carbon is independent of water depth, organic carbon mineralization and variable sedimentation rates. Instead, the pattern is predominantly controlled by sea ice-induced lateral transport and subsequent release in the Marginal Ice Zone (MIZ) as well as the distance to shore. Consistent with the observation of high vertical flux of particulate organic material in the MIZ, are amounts of marine organic carbon significantly enhanced in sediments below the winter ice margin. This is in accordance with modern observations suggesting that Arctic shelves with seasonal ice zones can be hot spots of vertical carbon export and thus a potential CO2 sink.